Proceeding of the 10th Asian Regional Maize Workshop. Makasar. 20-23 October 2008.
Formation of Maize Dihaploid Plant through Bulbosum Method
(I. Mariska, S. Hutami, M. Kosmiatin, G.S. Budiarti, and S. Utami)
Hybrid variety can improve maize productivity relatively higher than that of open polinated variety. One of alternative technology which used to speed up producing pure line was anther culture, but success of maize anther culture to produce haploid plant was still low. To anticipate the problem, BulBosum method will used in this research. The method was used to be done in Australia, Germany, adn New Zealand. One of parent of crossing in Bulbosum method was known as haploid inducer line which has RI-nj gene and expressed with anthocyanine color in seed from crossing. Haploid seeds produce from crossing identifying mark with purple color. Sexual crossing between non commercial line with anthocyanine color (No. 3059 and No. 3490) with Bisma, Sukmaraga, dan Arjuna varieties were done by BB Biogen in green house. Haploid embryos were isolated from the purple seeds and planted in MS, ½ MS, N6, and Yupei medium combined with GA3 (10, 30, and 50 mg/l). Chromosome analysis was done at tip of the roots. Result of sexual crossing showed that the highest presentage of purple seed formation was 31,58% which came from F1 (Bismax3490) with percentage of purple seed per ear was 1,5% with average of purple seed per ear was 3,82. Purple seeds germinated 100% in all germination medium. Result of chromosome showed that chromosome number of germinated purple seed was a half (20 ± 1) of that of germination yellow seed.