Prosiding Seminar

cov_prosiding2006Prosiding Seminar memuat hasil-hasil penelitian rintisan dan bioteknologi tanaman, serta himpunan makalah hasil-hasil penelitian yang disajikan dalam forum seminar, lokakarya maupun workshop yang diselenggarakan oleh BB Biogen. Berikut adalah daftar artikel yang telah diterbitkan dalam buku Prosiding Seminar.

2015

  1. Iskandar Mirza Darmadi and Ramlan. 2015. Pengelolaan Sumber Daya Genetik Tanaman Pangan di Provinsi Aceh. Prosiding seminar nasional sumber daya genetik pertanian 1-11.
    [BibTeX] [Abstract] [PDF: Pengelolaan Sumber Daya Genetik Tanaman Pangan di Provinsi Aceh ]
    Activities on genetic resources management programmed of Indonesian Agency for Agricultural Research and Development (AIAT) Aceh in 2013 focused on food crops that have long been cultivated by farmers in the provinces of Aceh and have been widely recognized. This study subjected to 1). Provide information of diversity of PGR (rice, soybeans, peanuts and other crops) which are maintained in home gardens, farmers and collection garden of Aceh province and 2). Providing information of status of PGR that can be used as reference to develop policies related to its management in the Aceh province. The inventory and collection of food crops in some districts of Aceh province resulted some local rice varieties i.e. Sigupai rice, Cantik Putih rice, Cantik Lembayung rice, Rom Kuring rice, Rom Putih rice, Rom Kuning rice, and Pulo rice. This activity was also resulted in collection of some legumes i.e. kipas putih soybean, kipas merah soybean, and peanuts. Characterization has been conducted for those the collection. In situ conservation conducted in the garden’s collection of IAIT Aceh.
    @article{andRamlan15p1,
    title = {{Pengelolaan Sumber Daya Genetik Tanaman Pangan di Provinsi Aceh}},
    author = {Iskandar Mirza, Darmadi, and Ramlan},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {1-11},
    year = {2015},
    abstract = {Activities on genetic resources management programmed of Indonesian Agency
    for Agricultural Research and Development (AIAT) Aceh in 2013 focused on food
    crops that have long been cultivated by farmers in the provinces of Aceh and have
    been widely recognized. This study subjected to 1). Provide information of
    diversity of PGR (rice, soybeans, peanuts and other crops) which are maintained in
    home gardens, farmers and collection garden of Aceh province and 2). Providing
    information of status of PGR that can be used as reference to develop policies
    related to its management in the Aceh province. The inventory and collection of
    food crops in some districts of Aceh province resulted some local rice varieties i.e.
    Sigupai rice, Cantik Putih rice, Cantik Lembayung rice, Rom Kuring rice, Rom
    Putih rice, Rom Kuning rice, and Pulo rice. This activity was also resulted in
    collection of some legumes i.e. kipas putih soybean, kipas merah soybean, and
    peanuts. Characterization has been conducted for those the collection. In situ
    conservation conducted in the garden's collection of IAIT Aceh.},
    keywords = {Genetic resources, local rice, local soybean, local peanut, garden
    collection},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/Prosiding Seminar Nasional SDG/1-Mirza - BPTP Aceh.pdf}
    }
  2. Dahono and Yayu Zurriyati. 2015. Inventarisasi Sumber Daya Genetik Tanaman di Pulau Karimun dan Pulau Kundur Kabupaten Karimun Provinsi Kepulauan Riau. Prosiding seminar nasional sumber daya genetik pertanian 12-20.
    [BibTeX] [Abstract] [PDF: Inventarisasi Sumber Daya Genetik Tanaman di Pulau Karimun dan Pulau Kundur Kabupaten Karimun Provinsi Kepulauan Riau ]
    To obtain information on levels of diversity and potential utilization of genetic resources an inventory surveys of plant genetic resources (PGR) has been conducted in Karimun Riau Islands Province (Karimun and Kundur) involving 12 farmers respondents. PGR were inventarized from their land yards and outside land yards. Data collection include, location where the PGR was discovered, name of the village, coordinates (N/S, W/E), crops (food, fruit, plantation, herb/spices), name of plant and main morphological description (fruit shape, color, and utilization). The data obtained were analyzed descriptively. The results showed that most of the PGR activities were on fruit trees. Of the PGR inventarization in Karimun Island, it was found 11 species, while in Kundur about 19 species were found. Several types of fruit trees such as durian, mangosteen, rambutan, mango, banana, jackfruit, and breadfruit had been used commercially by the farmers, while some other types (brown mango, kueni, guava, Rambai, tamarind, and cashew) had not well managed. The local PGR of Karimun Island Kundur district of Karimun belonging to crop plantation that had been used commercially include patchouli and rubber, while pecans, and sugar were not optimally well managed. The PGR belonging to the local forestry plants includes aloes, Sungkai and mahogany. The existence PGR seems to be sparse and there were no written information at garden PGR collection of relevant agencies. Respondents growing PGR still limited to an ornamentals plant yard or just a hobby, although some already sold these plants commercially
    @article{Dahono15p12,
    title = {{Inventarisasi Sumber Daya Genetik Tanaman di Pulau Karimun dan Pulau Kundur Kabupaten Karimun Provinsi Kepulauan Riau}},
    author = {Dahono and Yayu Zurriyati},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {12-20},
    year = {2015},
    abstract = {To obtain information on levels of diversity and potential utilization of genetic resources an inventory surveys of plant genetic resources (PGR) has been conducted in Karimun Riau Islands Province (Karimun and Kundur) involving 12 farmers respondents. PGR were inventarized from their land yards and outside land yards. Data collection include, location where the PGR was discovered, name of the village, coordinates (N/S, W/E), crops (food, fruit, plantation, herb/spices), name of plant and main morphological description (fruit shape, color, and utilization). The data obtained were analyzed descriptively. The results showed that most of the PGR activities were on fruit trees. Of the PGR inventarization in Karimun Island, it was found 11 species, while in Kundur about 19 species were found. Several types of fruit trees such as durian, mangosteen, rambutan, mango, banana, jackfruit, and breadfruit had been used commercially by the farmers, while some other types (brown mango, kueni, guava, Rambai, tamarind, and cashew) had not well managed. The local PGR of Karimun Island Kundur district of Karimun belonging to crop plantation that had been used commercially include patchouli
    and rubber, while pecans, and sugar were not optimally well managed. The PGR
    belonging to the local forestry plants includes aloes, Sungkai and mahogany. The
    existence PGR seems to be sparse and there were no written information at garden
    PGR collection of relevant agencies. Respondents growing PGR still limited to an
    ornamentals plant yard or just a hobby, although some already sold these plants
    commercially},
    keywords = {Biodiversity, plant genetic resources},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/2-Dahono - BPTP Kepulauan Riau.pdf}
    }
  3. Budi Raharjo Triyandar Arief, Renny Utami Somantri and Wiratno. 2015. Eksplorasi dan Karakterisasi Sumber Daya Genetik Lokal Tanaman Pangan dan Hortikultura Spesifik Lokasi Sumatera Selatan. Prosiding seminar nasional sumber daya genetik pertanian 21-31.
    [BibTeX] [Abstract] [PDF: Eksplorasi dan Karakterisasi Sumber Daya Genetik Lokal Tanaman Pangan dan Hortikultura Spesifik Lokasi Sumatera Selatan ]
    There are five agroecology zones in South Sumatera Province, i.e. lowlands, dry lands, uplands, tidal swamps, and irrigated lands. This condition indicates the presence of biodiversity. Information of biodiversity can be utilized as a basis for government policy formulation and public welfare. This research aimed to do inventory, explore, and characterize the genetic resources of food and horticultural crops in South Sumatera Province. The method used was a survey method by conducting an inventory, exploration, and characterization. Samples were selected by stratification. The selected samples were taken in each agroecology zone, at least 30 farmers. The results showed that 8 accessions of rice, 1 accession of tubers, and 8 accessions of fruit trees were acquired in lowlands; 14 accessions of rice and 4 accessions of fruit trees were obtained in uplands; 6 accessions of rice and 1 accessions of perennial plants were obtained in tidal land agroecosystem.
    @article{andWiratnop21,
    title = {{Eksplorasi dan Karakterisasi Sumber Daya Genetik Lokal Tanaman Pangan dan Hortikultura Spesifik Lokasi Sumatera Selatan}},
    author = {Budi Raharjo, Triyandar Arief, Renny Utami Somantri, and Wiratno},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {21-31},
    year = {2015},
    abstract = {There are five agroecology zones in South Sumatera Province, i.e. lowlands, dry lands, uplands, tidal swamps, and irrigated lands. This condition indicates the presence of biodiversity. Information of biodiversity can be utilized as a basis for government policy formulation and public welfare. This research aimed to do inventory, explore, and characterize the genetic resources of food and horticultural crops in South Sumatera Province. The method used was a survey method by conducting an inventory, exploration, and characterization. Samples were selected by stratification. The selected samples were taken in each agroecology zone, at least 30 farmers. The results showed that 8 accessions of rice, 1 accession of tubers, and 8 accessions of fruit trees were acquired in lowlands; 14 accessions of rice and 4 accessions of fruit trees were obtained in uplands; 6 accessions of rice and 1 accessions of perennial plants were obtained in tidal land agroecosystem.},
    keywords = {biodiversity, south sumatera, food crops, horticultures},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/3-Budi Raharjo - BPTP Sumsel.pdf}
    }
  4. Sri Rustini Tri Sudaryono, Joko Triastono and Intan Gilang Cempaka. 2015. Inventarisasi, Eksplorasi dan Upaya Koleksi Sumber Daya Genetik Tanaman Pangan Jawa Tengah. Prosiding seminar nasional sumber daya genetik pertanian 32-40.
    [BibTeX] [Abstract] [PDF: Inventarisasi, Eksplorasi dan Upaya Koleksi Sumber Daya Genetik Tanaman Pangan Jawa Tengah ]
    Central Java is one of the centers of diversity of plant genetic resources (PGR) in Indonesia. The potential of existing genetic resources should be utilized to meet human food sufficiency. The purpose of this activity is to inventory, explore, and ex situ collect crops in Central Java. Inventory and exploration was conducted from April to July 2003 in collaboration with relevant agencies in four districts i.e. Tegal, Brebes, Banjarnegara, and Karanganyar. This activity is carried out step-by step by relying on direct sources from the key person and from literature data. Respondents selected by taking thirty farmers from each agro ecological/administrative regions. The results of the inventory showed the highest number of accessions is rice (Oryza sativa), followed by cassava and taro (Colocasia esculenta and Xanthosoma sagitifolium). Whereas, minor Tubers as uwi (Dioscorea sp), suweg (Amorphophallus campanulatu) gembili (Dioscorea esculenta L.), ganyong (Canna edulis), and garut (Marantha arundinacea L.) and local Nuts, consisting of mungbeans and minor nuts were found in small cultivation. Of the four regions observed, Tegal and Brebes has the largest value of H ‘(Shannon index) 2,377, which indicates that this region has the highest number of species. Equitability Index Value (EH) of the four regions showed that the value of each region is smaller than one; indicates the dominance of a particular species. This dominance is especially noticeable in the Karanganyar. Meanwhile in Tegal- Brebes the dominance effect is less, indicated by the high value of H and the large value of EH. Possibly, this is because of cultivation in this region were quite diverse and almost evenly. The Sorenson coefficient (SC) between region very small indicating that the sampling in these three regions have different agro ecosystem. A total of 25–100% of the PGR inventoried are then collected for conservation.
    @article{andIntanGilangCempaka15p32,
    title = {{Inventarisasi, Eksplorasi dan Upaya Koleksi Sumber Daya Genetik Tanaman Pangan Jawa Tengah}},
    author = {Sri Rustini, Tri Sudaryono, Joko Triastono, and Intan Gilang Cempaka},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {32-40},
    year = {2015},
    abstract = {Central Java is one of the centers of diversity of plant genetic resources (PGR) in
    Indonesia. The potential of existing genetic resources should be utilized to meet
    human food sufficiency. The purpose of this activity is to inventory, explore, and
    ex situ collect crops in Central Java. Inventory and exploration was conducted from
    April to July 2003 in collaboration with relevant agencies in four districts i.e.
    Tegal, Brebes, Banjarnegara, and Karanganyar. This activity is carried out step-by
    step by relying on direct sources from the key person and from literature data.
    Respondents selected by taking thirty farmers from each agro ecological/administrative regions. The results of the inventory showed the highest number of
    accessions is rice (Oryza sativa), followed by cassava and taro (Colocasia
    esculenta and Xanthosoma sagitifolium). Whereas, minor Tubers as uwi
    (Dioscorea sp), suweg (Amorphophallus campanulatu) gembili (Dioscorea
    esculenta L.), ganyong (Canna edulis), and garut (Marantha arundinacea L.) and
    local Nuts, consisting of mungbeans and minor nuts were found in small
    cultivation. Of the four regions observed, Tegal and Brebes has the largest value of
    H '(Shannon index) 2,377, which indicates that this region has the highest number
    of species. Equitability Index Value (EH) of the four regions showed that the value
    of each region is smaller than one; indicates the dominance of a particular species.
    This dominance is especially noticeable in the Karanganyar. Meanwhile in Tegal-
    Brebes the dominance effect is less, indicated by the high value of H and the large
    value of EH. Possibly, this is because of cultivation in this region were quite
    diverse and almost evenly. The Sorenson coefficient (SC) between region very
    small indicating that the sampling in these three regions have different agro
    ecosystem. A total of 25–100% of the PGR inventoried are then collected for
    conservation.},
    keywords = {Inventory, exploration, food crops, Central Java},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/Prosiding Seminar Nasional SDG/4-Sri Rustini - BPTP Jawa Tengah.pdf}
    }
  5. Sudarmadi Purnomo Handoko, Thohir Zubaidi and Saiful Hosni. 2015. Kekayaan dan Keragaman Sumber Daya Genetik Tanaman Pangan Lokal pada Tiga Wilayah Kabupaten yang Berbeda Kultur Budayanya di Provinsi Jawa Timur. Prosiding seminar nasional sumber daya genetik pertanian 41-49.
    [BibTeX] [Abstract] [PDF: Kekayaan dan Keragaman Sumber Daya Genetik Tanaman Pangan Lokal pada Tiga Wilayah Kabupaten yang Berbeda Kultur Budayanya di Provinsi Jawa Timur ]
    Cultivation practices in a diverse and integrated agriculture, both in the yard or outside the yard, has long been done by farmers, and can be used as status monitor of richness and diversity of plant genetic resources of local food (PGRLF) in the field. This research is to determine the changes of PGRLF richness and diversity values occurring in Sumenep, Blitar and Tuban districts. Communities of these districts have a different culture based on dominant ethnics. The research was conducted by using the survey method targeting 23 households who have land outside the yard and the yard area between 350 m 2-8500 m 2 for each district. PGRLF diversity analysis approach Shannon Index (H ‘), while the degree of similarity in the structure of the species using Sorenson scale coefficient (SC). The study found that the number of species and accession of SDGTPL in and outside house yard were 57 species comprising of 165 accessions in Sumenep district, and 51 species comprising of 113 accessions in Tuban district, and 63 species comprising of 154 accessions in Blitar district. The PGRLF richness in three regions decreased about 22.3–35.8% compared to the conditions of 5–10 years ago. There was no Sorenson coefficient (SC) close to 1, meaning that the richness of SDGTPL of the two regions does not have same structure of species. It’s an indicator that the culture of the local community determines the type of PGRLF richness and diversity. PGRLF diversity in both districts is categorized as medium, except from the industrial group whose diversity is very low. Equitability indexes (EH) of crops in the two districts were close to the value of 1, indicating that the level of species distribution between households in the region is relatively the same.
    @article{andSaifulHosni15p41,
    title = {{Kekayaan dan Keragaman Sumber Daya Genetik Tanaman Pangan Lokal pada Tiga Wilayah Kabupaten yang Berbeda Kultur Budayanya di Provinsi Jawa Timur}},
    author = {Sudarmadi Purnomo, Handoko, Thohir Zubaidi, and Saiful Hosni},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {41-49},
    year = {2015},
    abstract = {Cultivation practices in a diverse and integrated agriculture, both in the yard or outside the yard, has long been done by farmers, and can be used as status monitor of richness and diversity of plant genetic resources of local food (PGRLF) in the field. This research is to determine the changes of PGRLF richness and diversity values occurring in Sumenep, Blitar and Tuban districts. Communities of these districts have a different culture based on dominant ethnics. The research was conducted by using the survey method targeting 23 households who have land outside the yard and the yard area between 350 m 2-8500 m 2 for each district. PGRLF diversity analysis approach Shannon Index (H '), while the degree of similarity in the structure of the species using Sorenson scale coefficient (SC). The study found that the number of species and accession of SDGTPL in and outside house yard were 57 species comprising of 165 accessions in Sumenep district, and 51 species comprising of 113 accessions in Tuban district, and 63 species
    comprising of 154 accessions in Blitar district. The PGRLF richness in three
    regions decreased about 22.3–35.8% compared to the conditions of 5–10 years ago.
    There was no Sorenson coefficient (SC) close to 1, meaning that the richness of
    SDGTPL of the two regions does not have same structure of species. It's an
    indicator that the culture of the local community determines the type of PGRLF
    richness and diversity. PGRLF diversity in both districts is categorized as medium,
    except from the industrial group whose diversity is very low. Equitability indexes
    (EH) of crops in the two districts were close to the value of 1, indicating that the
    level of species distribution between households in the region is relatively the
    same.},
    keywords = {Diversity index, genetic resources, local food crops, backyard,
    community culture},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/5-Sudarmadi Purnomo - BPTP Jawa Timur.pdf}
    }
  6. Sija, Patta and Muh. Asaad. 2015. Diversitas Sumber Daya Genetik Tanaman di Provinsi Gorontalo. Prosiding seminar nasional sumber daya genetik pertanian 50-59.
    [BibTeX] [Abstract] [PDF: Diversitas Sumber Daya Genetik Tanaman di Provinsi Gorontalo ]
    Agricultural genetic resources management program that includes the preservation and utilization requires the status and distribution information of plant genetic resources. Inventory of plant genetic resources needs to be done to get the information level of diversity of plant genetic resources in the province of Gorontalo. The study was conducted from June to September 2013 in the province of Gorontalo (Bolango Bone, North Gorontalo and Pohuwato districts). The research was done using a sample survey of 30 farmers respondents who selected using stratified sampling based on distance from the country site and e type of road. The inventory data was conducted on selected plant genetic resources belonging to farmers/ respondents, derived from the yard, outside yard and garden collection. Data analysis was done using the Shannon index (H), Equitability Index (E) and Sorensen index (IS). The result showed local plant genetic resources as much as 183 plants and 285 accessions was spread throughout Bolango Bone, Gorontalo and North Pohuwato districts. Diversity of plant genetic resources in Pohuwato was classified as high species diversity with Shannon index value of 3.8914, while in the District of North Gorontalo and Bone Bolango were classified as moderate with Shannon Index value of 2.4021 and 2.1553, respectively. Distribution of plant genetic resources types in Pohuwato areas was classified evenly with Equitability index value of 0.7586. Structure types of plant genetic resources among all districts were relatively equal with the value of Sorensen index (IS) ranges from 57.55–63.16%.
    @article{PattaSija15p50,
    title = {{Diversitas Sumber Daya Genetik Tanaman di Provinsi Gorontalo}},
    author = {Patta Sija and Muh. Asaad},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {50-59},
    year = {2015},
    abstract = {Agricultural genetic resources management program that includes the preservation and utilization requires the status and distribution information of plant genetic resources. Inventory of plant genetic resources needs to be done to get the information level of diversity of plant genetic resources in the province of Gorontalo. The study was conducted from June to September 2013 in the province of Gorontalo (Bolango Bone, North Gorontalo and Pohuwato districts). The research was done using a sample survey of 30 farmers respondents who selected using stratified sampling based on distance from the country site and e type of road. The inventory data was conducted on selected plant genetic resources belonging to farmers/ respondents, derived from the yard, outside yard and garden collection. Data analysis was done using the Shannon index (H), Equitability Index (E) and Sorensen index (IS). The result showed local plant genetic resources as much as 183 plants and 285 accessions was spread throughout Bolango Bone, Gorontalo and North Pohuwato districts. Diversity of plant genetic resources in Pohuwato was classified as high species diversity with Shannon index value of 3.8914, while in the District of North Gorontalo and Bone Bolango were classified as moderate with Shannon Index value of 2.4021 and 2.1553, respectively. Distribution of plant genetic resources types in Pohuwato areas was classified evenly with Equitability index value of 0.7586. Structure types of plant genetic resources among all districts were relatively equal with the value of Sorensen index
    (IS) ranges from 57.55–63.16%.},
    keywords = {Diversity, genetic resources, plant, Gorontalo},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/6-Patta Sija - BPTP Gorontalo.pdf}
    }
  7. Moh. Ismail Wahab Yayat Hidayat and Hermawati Cahyaningrum. 2015. Inventarisasi dan Keragaman Sumber Daya Genetik Tanaman Pangan Halmahera Barat dan Kota Tidore Kepulauan di Maluku Utara. Prosiding seminar nasional sumber daya genetik pertanian 60-67.
    [BibTeX] [Abstract] [PDF: Inventarisasi dan Keragaman Sumber Daya Genetik Tanaman Pangan Halmahera Barat dan Kota Tidore Kepulauan di Maluku Utara ]
    North Maluku is one of the Indonesian archipelagos located in the region known to cross the equator and become the potential diversity of genetic resources. The research aimed to conduct the inventory of crop genetic resources in West Halmahera and Kota Tidore Kepulauan. It was held from January to December 2013. The method used was a descriptive exploratory survey, field observations, and interviews. Based on results, the inventory had collected a number of 51 accessions of food crop genetic resources, i.e. 17 local accessions of upland rice, 2 accessions of maize, 12 accessions of cassava, 10 accessions of sweet potato, 3 accessions of peanut, and 3 accessions of taro. In addition, other crop genetic resources were obtained, i.e. 38 accessions of vegetable crops, 57 accessions of fruit crops, 17 accessions of medicinal herbs/spices, and 18 accessions of plantation crops. Level of diversity of food crops in West Halmahera was low, whereas Kota Tidore Kepulauan was classified as moderate with commodity equity level was high in all locations. West Halmahera and Kota Tidore Kepulauan have similar food commodities classified, i.e. maize, cassava, and sweet potato.
    @article{andHermawatiCahyaningrum15p60,
    title = {{Inventarisasi dan Keragaman Sumber Daya Genetik Tanaman Pangan Halmahera Barat dan Kota Tidore Kepulauan di Maluku Utara}},
    author = {Moh. Ismail Wahab, Yayat Hidayat, and Hermawati Cahyaningrum},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {60-67},
    year = {2015},
    abstract = {North Maluku is one of the Indonesian archipelagos located in the region known to cross the equator and become the potential diversity of genetic resources. The research aimed to conduct the inventory of crop genetic resources in West Halmahera and Kota Tidore Kepulauan. It was held from January to December 2013. The method used was a descriptive exploratory survey, field observations, and interviews. Based on results, the inventory had collected a number of 51 accessions of food crop genetic resources, i.e. 17 local accessions of upland rice, 2 accessions of maize, 12 accessions of cassava, 10 accessions of sweet potato, 3 accessions of peanut, and 3 accessions of taro. In addition, other crop genetic resources were obtained, i.e. 38 accessions of vegetable crops, 57 accessions of fruit crops, 17 accessions of medicinal herbs/spices, and 18 accessions of plantation crops. Level of diversity of food crops in West Halmahera was low, whereas Kota Tidore Kepulauan was classified as moderate with commodity equity level was high in all locations. West Halmahera and Kota Tidore Kepulauan have similar food commodities classified, i.e. maize, cassava, and sweet potato.},
    keywords = {Inventory, diversity, genetic resources, food crop, North Maluku},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/7-Ismail Wahab - BPTP Malut.pdf}
    }
  8. Simatupang, S.. 2015. Preferensi Petani terhadap Varietas Baru Padi di Kabupaten Toba Samosir. Prosiding seminar nasional sumber daya genetik pertanian 68-74.
    [BibTeX] [Abstract] [PDF: Preferensi Petani terhadap Varietas Baru Padi di Kabupaten Toba Samosir ]
    Tobasamosir district had planted about 20 thousand hectares of paddy in 2011. The dominant variety grown was Ciherang. In the 2011 Planting Season I, a pilot project consisting of several new AARD-rice varieties namely: Inpari 1, Inpari 3, Inpari 4, Inpari 10, Inpari 13, Mekongga, and Sarinah has been introduced at the village Paindoan covering an area of 1 ha. The demonstration plots were done in farmer’s area. The neighboring plots were farmers’ plots who plant other rice varieties such as Ciherang, si Pandan and Boru Pardede. To know preferences of farmers, a survey study had been conducted at such village against farmer cooperator (21 farmers respondent), who grown themselves of these varieties and the neighboring farmers, who observed the development of new rice varieties. The results showed the preference of new varieties such as Mekongga, Sarinah, and Inpari 1 was higher. More than 90% of farmers were enthusiast to plant cv.Inpari 3, Inpari 4 and Inpari 10 in the next subsequent season. The preference of farmer to Inpari 13 was lower (only 30%).
    @article{Simatupang15p68,
    title = {{Preferensi Petani terhadap Varietas Baru Padi di Kabupaten Toba Samosir}},
    author = {S. Simatupang},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {68-74},
    year = {2015},
    abstract = {Tobasamosir district had planted about 20 thousand hectares of paddy in 2011. The dominant variety grown was Ciherang. In the 2011 Planting Season I, a pilot project consisting of several new AARD-rice varieties namely: Inpari 1, Inpari 3, Inpari 4, Inpari 10, Inpari 13, Mekongga, and Sarinah has been introduced at the village Paindoan covering an area of 1 ha. The demonstration plots were done in farmer's area. The neighboring plots were farmers' plots who plant other rice varieties such as Ciherang, si Pandan and Boru Pardede. To know preferences of farmers, a survey study had been conducted at such village against farmer cooperator (21 farmers respondent), who grown themselves of these varieties and the neighboring farmers, who observed the development of new rice varieties. The results showed the preference of new varieties such as Mekongga, Sarinah, and Inpari 1 was higher. More than 90% of farmers were enthusiast to plant cv.Inpari 3, Inpari 4 and Inpari 10 in the next subsequent season. The preference of farmer to Inpari 13 was lower (only 30%).},
    keywords = {rice, preferences, new varieties of rice},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/8-Sortha Simatupang - BPTP Sumatera Utara.pdf}
    }
  9. Julistia Bobihoe Desi Hernita and Endrizal. 2015. Pengelolaan Sumber Daya Genetik Tanaman Padi Spesifik Jambi. Prosiding seminar nasional sumber daya genetik pertanian 75-79.
    [BibTeX] [Abstract] [PDF: Pengelolaan Sumber Daya Genetik Tanaman Padi Spesifik Jambi ]
    Local rice is one of the plant genetic resources (PGR) which is quite diverse in the lowlands, medium-high, upland, lowland and tidal swamps. Inventory and identification of local rice are not yet done completely, while the existence sources of local rice germplasm is getting rare. Eploration activities was carried out at seven districts/cities i.e; the city of Jambi, Muaro Jambi, Batanghari, Tanjung Jabung Barat, Tanjung Jabung Timur, Merangin and Bungo from April to July 2013. Based on this exploration, it can be inventoried 95 types of local rice and mosty from lowland swamp, which is subsequently collected in the Assesment Institute for Agricultural Technology-Jambi and Indonesian Rice Research Institute Sukamandi. Inventarization of local rice in Jambi Province is also very useful to meet the need of social, economic, environment, science and technology aspects.
    @article{andEndrizal15p75,
    title = {{Pengelolaan Sumber Daya Genetik Tanaman Padi Spesifik Jambi}},
    author = {Julistia Bobihoe, Desi Hernita, and Endrizal},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {75-79},
    year = {2015},
    abstract = {Local rice is one of the plant genetic resources (PGR) which is quite diverse in the
    lowlands, medium-high, upland, lowland and tidal swamps. Inventory and
    identification of local rice are not yet done completely, while the existence sources
    of local rice germplasm is getting rare. Eploration activities was carried out at
    seven districts/cities i.e; the city of Jambi, Muaro Jambi, Batanghari, Tanjung
    Jabung Barat, Tanjung Jabung Timur, Merangin and Bungo from April to July
    2013. Based on this exploration, it can be inventoried 95 types of local rice and
    mosty from lowland swamp, which is subsequently collected in the Assesment
    Institute for Agricultural Technology-Jambi and Indonesian Rice Research Institute
    Sukamandi. Inventarization of local rice in Jambi Province is also very useful to
    meet the need of social, economic, environment, science and technology aspects.},
    keywords = {pgr, pgr inventory, local rice, pgr collection},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/9-Julistia Bobihoe - BPTP Jambi.pdf}
    }
  10. Arif Susila Sri Rustini, Endang Rohman Intan Gilang Cempaka and Vina Eka Prasetya. 2015. Kekerabatan Kultivar Padi Lokal Jawa Tengah Berdasarkan Karakter Agronomi dan Morfologi. Prosiding seminar nasional sumber daya genetik pertanian 80-89.
    [BibTeX] [Abstract] [PDF: Kekerabatan Kultivar Padi Lokal Jawa Tengah Berdasarkan Karakter Agronomi dan Morfologi ]
    An effort to develop superior new improved variety has been done by the government to support national rice self-sufficiency; hence the germplasm collections are one of the important points to support plant breeding program. The Assessment Institute for Agricultural Technology (AIAT) of Central Java had been successfully collected 58 local rice collected from exploration in Central Java regions. These collections are needed to develop high yielding cultivar as well as to determine their agronomic and morphological characteristics. Based on those characters, a research was aimed to assess the phylogenetic relationship among 58 collections of local rice. The research was conducted at Batang Experimental Station, Assessment Institute for Agricultural Technology (AIAT) of Central Java from June to Oct 2013. The experiment was conducted using cluster analysis based on differences on their agronomic and morphological traits. Based on the similarity level at 62.36%, it was formed 4 group clusters. Cluster I consisted of 32 cultivars (L1, L27, L46, L12, L31, L44, L47, L48, L17, L23, L16, L19, L18, L20, L4, L30, L26, L7, L13, L21, L40, L60, L50, L49, L61, L43, L54, L62, L25, L42, L51, L45), Cluster II consists of 24 cultivars (L2, L11, L14, L52, L22, L41, L28, L59, L53, L9, L37, L34, L3, L5, L6, L56, L57, L15, L24, L8, L39, L38, L29, L33), cluster III consists of L58 and cluster IV (cultivar L10).
    @article{andVinaEkaPrasetya15p80,
    title = {{Kekerabatan Kultivar Padi Lokal Jawa Tengah Berdasarkan Karakter Agronomi dan Morfologi}},
    author = {Arif Susila, Sri Rustini, Endang Rohman, Intan Gilang Cempaka, and Vina Eka Prasetya},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {80-89},
    year = {2015},
    abstract = {An effort to develop superior new improved variety has been done by the government to support national rice self-sufficiency; hence the germplasm collections are one of the important points to support plant breeding program. The Assessment Institute for Agricultural Technology (AIAT) of Central Java had been successfully collected 58 local rice collected from exploration in Central Java regions. These collections are needed to develop high yielding cultivar as well as to determine their agronomic and morphological characteristics. Based on those characters, a research was aimed to assess the phylogenetic relationship among 58 collections of local rice. The research was conducted at Batang Experimental Station, Assessment Institute for Agricultural Technology (AIAT) of Central Java from June to Oct 2013. The experiment was conducted using cluster analysis based on differences on their agronomic and morphological traits. Based on the similarity level at 62.36%, it was formed 4 group clusters. Cluster I consisted of 32 cultivars (L1, L27, L46, L12, L31, L44, L47, L48, L17, L23, L16, L19, L18, L20, L4, L30, L26, L7, L13, L21, L40, L60, L50, L49, L61, L43, L54, L62, L25, L42, L51, L45), Cluster II consists of 24 cultivars (L2, L11, L14, L52, L22, L41, L28, L59, L53, L9, L37, L34, L3, L5, L6, L56, L57, L15, L24, L8, L39, L38, L29, L33), cluster III consists of L58 and cluster IV (cultivar L10).},
    keywords = {local rice, genetic relationship, agronomic, morphological traits},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/10-Arif Susila - BPTP Jawa Tengah.pdf}
    }
  11. Kristamtini Setyorini Widyayanti, Sutarno and Sudarmaji. 2015. Keragaman Genetik Lima Kultivar Lokal Padi Beras Hitam Asal Yogyakarta Berdasarkan Karakter Morfologi. Prosiding seminar nasional sumber daya genetik pertanian 90-100.
    [BibTeX] [Abstract] [PDF: Keragaman Genetik Lima Kultivar Lokal Padi Beras Hitam Asal Yogyakarta Berdasarkan Karakter Morfologi ]
    Black rice is one of the local cultivars from Yogyakarta which have the superiority in terms of anthocyanins content causing purple to black pericarp rice-color. The research was carried out to identify genetic diversity of five black rice landraces originated from Yogyakarta based on morphological characteristics on both of vegetative and generative growth stage in accordance with System Characterization and Evaluation of Plant Guide, namely plant height, tillering ability, number of productive tillers, plant age, stem color, leg color, leaf color, leaf length, leaf width, ear leaf color, ligula leaf color, ligula shape, leaf angle, flag leaf angle, grain color, grain shape, brown rice length, brown rice form, panicle type, hair loss, secondary branches panicle, number of filled grains per panicle, pericarp color and aleurone color. Based on agromorphology data, the numerical data (plant height, number of productive tillers, grain length, grain width, plant age and number of filled grain per panicle) were analyzed to determine their genetic variability represented by GCV (Genetic Coefficient Variability) value, and it was further analysed using clustering analysis to known their relationships. The results showed that plant height and number of productive tillers characteristics had a broad genetic variability, whereas characters of grain length, grain width, number of filled grain per panicle and plant age had a narrow genetic variability. Based on morphological characteristics, there were three groups of black rice landraces-Yogyakarta, namely group I (black rice of Melik and Pari ireng); group II (Cempo ireng and Jlitheng rice black rice landraces) and group III of black rice cultivars from Bantul.
    @article{andSudarmaji15p90,
    title = {{Keragaman Genetik Lima Kultivar Lokal Padi Beras Hitam Asal Yogyakarta Berdasarkan Karakter Morfologi}},
    author = {Kristamtini, Setyorini Widyayanti, Sutarno, and Sudarmaji},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {90-100},
    year = {2015},
    abstract = {Black rice is one of the local cultivars from Yogyakarta which have the superiority
    in terms of anthocyanins content causing purple to black pericarp rice-color. The
    research was carried out to identify genetic diversity of five black rice landraces
    originated from Yogyakarta based on morphological characteristics on both of
    vegetative and generative growth stage in accordance with System Characterization
    and Evaluation of Plant Guide, namely plant height, tillering ability, number of
    productive tillers, plant age, stem color, leg color, leaf color, leaf length, leaf width,
    ear leaf color, ligula leaf color, ligula shape, leaf angle, flag leaf angle, grain color,
    grain shape, brown rice length, brown rice form, panicle type, hair loss, secondary
    branches panicle, number of filled grains per panicle, pericarp color and aleurone
    color. Based on agromorphology data, the numerical data (plant height, number of
    productive tillers, grain length, grain width, plant age and number of filled grain
    per panicle) were analyzed to determine their genetic variability represented by
    GCV (Genetic Coefficient Variability) value, and it was further analysed using
    clustering analysis to known their relationships. The results showed that plant height and number of productive tillers characteristics had a broad genetic
    variability, whereas characters of grain length, grain width, number of filled grain
    per panicle and plant age had a narrow genetic variability. Based on morphological
    characteristics, there were three groups of black rice landraces-Yogyakarta, namely
    group I (black rice of Melik and Pari ireng); group II (Cempo ireng and Jlitheng
    rice black rice landraces) and group III of black rice cultivars from Bantul.},
    keywords = {Genetic variability, morphology, black rice, local Yogyakarta},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/11-Kristamtini 1 - BPTP Yogyakarta.pdf}
    }
  12. Kristamtini Setyorini Widyayanti, Sutarno Sudarmaji and Endang Wisnu Wiranti. 2015. Pelestarian Partisipatif Padi Beras Hitam Lokal di Yogyakarta. Prosiding seminar nasional sumber daya genetik pertanian 101-109.
    [BibTeX] [Abstract] [PDF: Pelestarian Partisipatif Padi Beras Hitam Lokal di Yogyakarta ]
    Plant germplasms is one of biodiversity richness that needs to be managed properly for its usefulness and extinction avoidance. In addition to natural events, diversity extinction may occur by competition of new cultivars that could take over local cultivars. The endangered local cultivars which threatened for its existence is local black rice. Many black rice have different names in each region. The types of black rice has the same genetic character yet or just a different name hence to know the cultivar variability in each specific agro-climate and the conservation effort are need to be done. Germplasm preservation cannot be done by the government alone; but need participatory breeding involving continuous and sustainable efforts among government (researcher), farmers, traders and consumers. Farmer participatory breeding study was carried out by selection of grain (rice mixed with black- owned), multiplication of black rice seed, doing the function as a conservationist as well as being a paddy black rice-trader. Through these efforts, demand of black rice product of farmers in Sleman, had increased in January to July 2010, followed by extensification of black rice-land areas. The conservation of black rice cultivars, besides increasing genetic resources, it also increase farmers financial. Based on the research, black rice had a benefit ratio (B/C) of 1.58, so that black rice cultivation is feasible to be developed especially in Yogyakarta.
    @article{andEndangWisnuWiranti15p101,
    title = {{Pelestarian Partisipatif Padi Beras Hitam Lokal di Yogyakarta}},
    author = {Kristamtini, Setyorini Widyayanti, Sutarno, Sudarmaji, and Endang Wisnu Wiranti},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {101-109},
    year = {2015},
    abstract = {Plant germplasms is one of biodiversity richness that needs to be managed properly for its usefulness and extinction avoidance. In addition to natural events, diversity extinction may occur by competition of new cultivars that could take over local cultivars. The endangered local cultivars which threatened for its existence is local black rice. Many black rice have different names in each region. The types of black rice has the same genetic character yet or just a different name hence to know the cultivar variability in each specific agro-climate and the conservation effort are need to be done. Germplasm preservation cannot be done by the government alone; but need participatory breeding involving continuous and sustainable efforts among government (researcher), farmers, traders and consumers. Farmer participatory breeding study was carried out by selection of grain (rice mixed with black- owned), multiplication of black rice seed, doing the function as a conservationist as well as being a paddy black rice-trader. Through these efforts, demand of black rice product of farmers in Sleman, had increased in January to July 2010, followed by extensification of black rice-land areas. The conservation of black rice cultivars, besides increasing genetic resources, it also increase farmers financial. Based on
    the research, black rice had a benefit ratio (B/C) of 1.58, so that black rice
    cultivation is feasible to be developed especially in Yogyakarta.},
    keywords = {Preservation, participatory breeding, black rice, Yogyakarta},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/12-Kristamtini 2 - BPTP Yogyakarta.pdf}
    }
  13. S.A.N. Aryawati A.A.N.B. Kamandalu and Dana I. G. K. Arsana. 2015. Keragaan Genetik Galur Mutan Harapan Padi Sawah di Subak Guama, Desa Selanbawak, Kecamatan Marga, Bali. Prosiding seminar nasional sumber daya genetik pertanian 110-117.
    [BibTeX] [Abstract] [PDF: Keragaan Genetik Galur Mutan Harapan Padi Sawah di Subak Guama, Desa Selanbawak, Kecamatan Marga, Bali ]
    Plants Breeding with aided by mutation is quite popular technique to produce variations of new improved varieties. The role of technological innovation on high yielding varieties are very large in efforts to increase national rice production. Through the activities of BATAN-paddy mutant multilocation trials was done in order to generate candidate varieties that will be recommended as a specific high yielding varieties. This research has been conducted in Subak Guama, Selanbawak Village, Marga District, Bali from November 2012 to March 2013. The study was arranged in a randomized block design with 14 treatments (11 mutants + 3 varieties) with three replications. The treatments were: lines UDS 011, UDS 012, UDS 013, UDS 014, UDS 015, UDS 016, UDS 017, UDS 018, UDS 019, UDS 020, UDS 021, 237 KI, KI 432, and Ciherang (control). Plant parameters measured were age 50% of flowering plants, plant height, number of tillers, number of grains per panicle and empty panicle/hills, 1000 seeds weight and dry milled grain yield per hectare. Data were analyzed by analysis of variance (Annova) and LSD at 5% level. Mutant lines tested showed significant differences in all variables observed, except for 1000 seeds weight. The UDS 016 line treatment showed the highest production of 7.67 tons/ha of grain higher than that of check varieties namely KI 237 (5.10 t/ha), KI 432 (6.04 t/ha), and Ciherang (5.85 t/ha).
    @article{DanaArsana15p110,
    title = {{Keragaan Genetik Galur Mutan Harapan Padi Sawah di Subak Guama, Desa Selanbawak, Kecamatan Marga, Bali}},
    author = {S.A.N. Aryawati, A.A.N.B. Kamandalu, and I.G.K. Dana Arsana},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {110-117},
    year = {2015},
    abstract = {Plants Breeding with aided by mutation is quite popular technique to produce variations of new improved varieties. The role of technological innovation on high yielding varieties are very large in efforts to increase national rice production. Through the activities of BATAN-paddy mutant multilocation trials was done in order to generate candidate varieties that will be recommended as a specific high yielding varieties. This research has been conducted in Subak Guama, Selanbawak Village, Marga District, Bali from November 2012 to March 2013. The study was arranged in a randomized block design with 14 treatments (11 mutants + 3 varieties) with three replications. The treatments were: lines UDS 011, UDS 012, UDS 013, UDS 014, UDS 015, UDS 016, UDS 017, UDS 018, UDS 019, UDS 020, UDS 021, 237 KI, KI 432, and Ciherang (control). Plant parameters measured were age 50% of flowering plants, plant height, number of tillers, number of grains per panicle and empty panicle/hills, 1000 seeds weight and dry milled grain yield per hectare. Data were analyzed by analysis of variance (Annova) and LSD at 5% level. Mutant lines tested showed significant differences in all variables observed, except for 1000 seeds weight. The UDS 016 line treatment showed the highest production of 7.67 tons/ha of grain higher than that of check varieties namely KI 237 (5.10 t/ha), KI 432 (6.04 t/ha), and Ciherang (5.85 t/ha).},
    keywords = {performance, genetic mutant, paddy},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/13-SAN Aryati - BPTP Bali.pdf}
    }
  14. Subekti, A.. 2015. Karakteristik dan Pola Kekerabatan Plasma Nutfah Padi Beras Merah di Kalimantan Barat. Prosiding seminar nasional sumber daya genetik pertanian 118-125.
    [BibTeX] [Abstract] [PDF: Karakteristik dan Pola Kekerabatan Plasma Nutfah Padi Beras Merah di Kalimantan Barat ]
    Brown rice is known to contain a lot of fiber, protein, iron, potassium, vitamin E and a trace of sugar. Brown rice is very good to be consumed because it can reduce the risk of diabetes and other degenerative diseases. Taking into account the health benefits of brown rice, it is necessary to do an inventory of local rice brown rice. This research aimed to inventory, characterize and determine relationships pattern of local rice brown rice germplasm in West Kalimantan. The study was conducted using a survey method in five districts i.e. Bengkayang, Hedgehogs, Pontianak, Sanggau, and Kapuas Hulu. The results study showed that most of local brown rice was found in Sanggau district as many as 3 accessions. Based on morphological characteristics of seeds and grains, there was variation among local brown rice encountered. From the cluster analysis based on 7 morphological characters, it can be noted that there were two groups of local rice germplasm brown rice in West Kalimantan. The first group consisted of Serai, Beras Merah Pontianak, Darah Belut, Kapuas, Uwi, Merah Besar, Beras Merah Landak and C-4. The second group consisted of Dolog, Serendah Merah, Sanik Merah and Dekor.
    @article{Subekti15p118,
    title = {{Karakteristik dan Pola Kekerabatan Plasma Nutfah Padi Beras Merah di Kalimantan Barat}},
    author = {A. Subekti},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {118-125},
    year = {2015},
    abstract = {Brown rice is known to contain a lot of fiber, protein, iron, potassium, vitamin E and a trace of sugar. Brown rice is very good to be consumed because it can reduce the risk of diabetes and other degenerative diseases. Taking into account the health benefits of brown rice, it is necessary to do an inventory of local rice brown rice. This research aimed to inventory, characterize and determine relationships pattern of local rice brown rice germplasm in West Kalimantan. The study was conducted using a survey method in five districts i.e. Bengkayang, Hedgehogs, Pontianak, Sanggau, and Kapuas Hulu. The results study showed that most of local brown rice was found in Sanggau district as many as 3 accessions. Based on morphological characteristics of seeds and grains, there was variation among local brown rice encountered. From the cluster analysis based on 7 morphological characters, it can be noted that there were two groups of local rice germplasm brown rice in West Kalimantan. The first group consisted of Serai, Beras Merah Pontianak, Darah Belut, Kapuas, Uwi, Merah Besar, Beras Merah Landak and C-4. The second group consisted of Dolog, Serendah Merah, Sanik Merah and Dekor.},
    keywords = {characteristics, relationships pattern, germplasm, brown rice},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/14-Agus Subekti - BPTP Kalimantan Barat.pdf}
    }
  15. Boy, Ruslan and Soeharsono. 2015. Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman padi di Kabupaten Banggai. Prosiding seminar nasional sumber daya genetik pertanian 126-133.
    [BibTeX] [Abstract] [PDF: Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman padi di Kabupaten Banggai ]
    The district of Banggai possesses a high diversity of genetic resources, one of which is rice germplasm. This study was aimed to inventory and to identify rice landrace in order to obtain information of agronomic characters and its potential so it can be further used in breeding programs. The study was conducted on June 2013 at seven sub districts in Banggai i.e. Kintom, Batui, South Batui, East Luwuk, Masama, Balantak and South Balantak. The study was conducted using a survey method and sampling conducted by Snow-Ball Sampling technique. The farmer that used as respondent was selected based on the stratification. Plant data recorded including local name, the type of rice, the taste, dan the utilization. Rice accessions were collected from this activity. Based on its agro-ecosystem, these accession were grouped into two types namely wetland rice which is consisted of 4 accessions and upland rice which is consisted of 62 accessions.
    @article{RuslanBoy15p126,
    title = {{Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman padi di Kabupaten Banggai}},
    author = {Ruslan Boy and Soeharsono},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {126-133},
    year = {2015},
    abstract = {The district of Banggai possesses a high diversity of genetic resources, one of which is rice germplasm. This study was aimed to inventory and to identify rice landrace in order to obtain information of agronomic characters and its potential so it can be further used in breeding programs. The study was conducted on June 2013 at seven sub districts in Banggai i.e. Kintom, Batui, South Batui, East Luwuk, Masama, Balantak and South Balantak. The study was conducted using a survey method and sampling conducted by Snow-Ball Sampling technique. The farmer that used as respondent was selected based on the stratification. Plant data recorded including local name, the type of rice, the taste, dan the utilization. Rice accessions were collected from this activity. Based on its agro-ecosystem, these accession were grouped into two types namely wetland rice which is consisted of 4 accessions and upland rice which is consisted of 62 accessions.},
    keywords = {inventory, germplasm, rice, banggai},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/15-Ruslan Boy - BPTP Sulteng-Padi.pdf}
    }
  16. Sahardi Herniwati and Fadjry Djufry. 2015. Keragaman Karakter Morfologis Palsma Nutfah Padi Lokal Dataran Tinggi Tana Toraja, Sulawesi Selatan. Prosiding seminar nasional sumber daya genetik pertanian 134-143.
    [BibTeX] [Abstract] [PDF: Keragaman Karakter Morfologis Palsma Nutfah Padi Lokal Dataran Tinggi Tana Toraja, Sulawesi Selatan ]
    Aim of the research was to know morphological character diversity of local rice germplasm of Tana Toraja highlands of South Sulawesi.The diversity of morphological characteristics of a plant can be used by plant breeders to produce new variety. Material for morphology characterization derived from the results of exploration conducted in in February 2013, in the Tagari village, Balusu sub district, North Tana Toraja dirictst. The region located at an altitude of 880 meters sea level above. These Activities was conducted from April to September 2013. Twenty nine of rice local varieties germplasm as a material for characterization. They were planted each three rows with 45 clumps. Observation of morphological characters were used to complete descripstion of each variety. To determine the phylogenetic relationship of the local varieties was made by Dendrogram Analysis. Data collected included maturity, plant height, number of productive tillers, 1.000 grain weight (g), production (t/ha), plant shape, leg color, stem color, leaf color, the position of the flag leaf, leaf position, grain shape, grain color, loss grain and feathers/tail on the end grain. Results showed morphological diversity of local rice crop germplasm Tana Toraja highlands was very high, such as harvesting varied from 135 days (Pare Ambo) to 180 days (Pare Lea), plant height from 120 cm (Pare Kaloko)-167 cm (Pare Tallang-2), the number of productive tillers from 7.6 (Pare Tallang-2) to 14.6 (Pare Tallang-1), 1000 grain weight from 17.6 grams t (Pare Lotong Tanduk)-45.9 grams (Pare Pulu Tille), and average of productivity varied of 6.53 t/ha (Pare Lalodo-1) to 2.53 (Pare Tallang-2). Relationship based on morphological characters of 29 local rice varetas Tana Toraja were divided into three main clusters. Pare Kaloko variety has the most distant compared to other samples of local varieties
    @article{andFadjryDjufry15p134,
    title = {{Keragaman Karakter Morfologis Palsma Nutfah Padi Lokal Dataran Tinggi Tana Toraja, Sulawesi Selatan}},
    author = {Sahardi, Herniwati, and Fadjry Djufry},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {134-143},
    year = {2015},
    abstract = {Aim of the research was to know morphological character diversity of local rice germplasm of Tana Toraja highlands of South Sulawesi.The diversity of morphological characteristics of a plant can be used by plant breeders to produce new variety. Material for morphology characterization derived from the results of exploration conducted in in February 2013, in the Tagari village, Balusu sub district, North Tana Toraja dirictst. The region located at an altitude of 880 meters sea level above. These Activities was conducted from April to September 2013. Twenty nine of rice local varieties germplasm as a material for characterization. They were planted each three rows with 45 clumps. Observation of morphological characters were used to complete descripstion of each variety. To determine the phylogenetic relationship of the local varieties was made by Dendrogram Analysis. Data collected included maturity, plant height, number of productive tillers, 1.000 grain weight (g), production (t/ha), plant shape, leg color, stem color, leaf color, the position of the flag leaf, leaf position, grain shape, grain color, loss grain and feathers/tail on the end grain. Results showed morphological diversity of local rice
    crop germplasm Tana Toraja highlands was very high, such as harvesting varied
    from 135 days (Pare Ambo) to 180 days (Pare Lea), plant height from 120 cm
    (Pare Kaloko)-167 cm (Pare Tallang-2), the number of productive tillers from 7.6
    (Pare Tallang-2) to 14.6 (Pare Tallang-1), 1000 grain weight from 17.6 grams t
    (Pare Lotong Tanduk)-45.9 grams (Pare Pulu Tille), and average of productivity
    varied of 6.53 t/ha (Pare Lalodo-1) to 2.53 (Pare Tallang-2). Relationship based on
    morphological characters of 29 local rice varetas Tana Toraja were divided into
    three main clusters. Pare Kaloko variety has the most distant compared to other
    samples of local varieties},
    keywords = {Diversity, morphological characters, local rice},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/16-Sahardi - BPTP Sulawesi Selatan.pdf}
    }
  17. Slamet Hartanto Yayat Hidayat and Indra H. Hendaru. 2015. Eksplorasi Plasma Nutfah Padi Lokal di Kabupaten Halmahera Utara, Maluku Utara. Prosiding seminar nasional sumber daya genetik pertanian 144-150.
    [BibTeX] [Abstract] [PDF: Eksplorasi Plasma Nutfah Padi Lokal di Kabupaten Halmahera Utara, Maluku Utara ]
    Potential genetic resources (SDG) in North Halmahera rice is very high, especially rice fields, because nearly 90% of cultivated paddy fields farmers are local varieties. But information about the genetic diversity of local rice North Halmahera has not been widely reported. The aims of this research is to explore, inventory and physical observations of the performance of local rice in North Halmahera district. Exploration conducted in April-May 2014 in North Halmahera district. The method used is purposive survey in 9 of 17 districts as the center of the rice fields. The results found four rice dry land cultivated namely Red, Molulu rice, paddy and rice Taraudu Manyanyi. Red rice productivity and Taraudu local community level reaches 1.5-2 t/ha, while the paddy Manyanyi reach 1 t/ha with harvest age 4-5 months. Molulu rice characterized as adaptive local climate change because it can live in rushing streams and on dry land.
    @article{Hendaru15p144,
    title = {{Eksplorasi Plasma Nutfah Padi Lokal di Kabupaten Halmahera Utara, Maluku Utara}},
    author = {Slamet Hartanto, Yayat Hidayat, and Indra H. Hendaru},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {144-150},
    year = {2015},
    abstract = {Potential genetic resources (SDG) in North Halmahera rice is very high, especially rice fields, because nearly 90% of cultivated paddy fields farmers are local varieties. But information about the genetic diversity of local rice North Halmahera has not been widely reported. The aims of this research is to explore, inventory and physical observations of the performance of local rice in North Halmahera district. Exploration conducted in April-May 2014 in North Halmahera district. The method used is purposive survey in 9 of 17 districts as the center of the rice fields. The results found four rice dry land cultivated namely Red, Molulu rice, paddy and rice Taraudu Manyanyi. Red rice productivity and Taraudu local community level reaches 1.5-2 t/ha, while the paddy Manyanyi reach 1 t/ha with harvest age 4-5 months. Molulu rice characterized as adaptive local climate change because it can live in rushing streams and on dry land.},
    keywords = {exploration, paddy field, north halmahera},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/17-Slamet Hartanto - BPTP Maluku Utara.pdf}
    }
  18. Boy, Ruslan and Soeharsono. 2015. Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman Umbi-umbian di Kabupaten Banggai Kepulauan. Prosiding seminar nasional sumber daya genetik pertanian 151-159.
    [BibTeX] [Abstract] [PDF: Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman Umbi-umbian di Kabupaten Banggai Kepulauan ]
    This study aimed to inventory and identification the accessions of tubers germplasm to obtain information about its advantages and agronomic characteristic, so it can be used in breeding programs. The study was conducted in five sub districts in Banggai Islands; Tinangkung, North Tinangkung, South Tinangkung, Totikum, South Peling and Central Peling, in May 2013. The study was a non-experimental study, data were collected from observations and interviews by visiting farmhouses who decided by sampling method. Number of sample is 30 respondents. Based on this study, there are six tubers germplasm, consisting of 44 types of Ubi Banggai, 11 types of talas, one type of keladi, 16 species of gembili, 8 species of cassava and 13 types of sweet potato.
    @article{RuslanBoy15p151,
    title = {{Inventarisasi dan Identifikasi Sumber Daya Genetik Tanaman Umbi-umbian di Kabupaten Banggai Kepulauan}},
    author = {Ruslan Boy and Soeharsono},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {151-159},
    year = {2015},
    abstract = {This study aimed to inventory and identification the accessions of tubers germplasm to obtain information about its advantages and agronomic characteristic, so it can be used in breeding programs. The study was conducted in five sub districts in Banggai Islands; Tinangkung, North Tinangkung, South Tinangkung, Totikum, South Peling and Central Peling, in May 2013. The study was a non-experimental study, data were collected from observations and interviews by visiting farmhouses who decided by sampling method. Number of sample is 30 respondents. Based on this study, there are six tubers germplasm, consisting of 44 types of Ubi Banggai, 11 types of talas, one type of keladi, 16 species of gembili, 8 species of cassava and 13 types of sweet potato.},
    keywords = {inventory, germplasm, tubers},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/18-Ruslan Boy - BPTP Sulteng-Ubi-ubian.pdf}
    }
  19. Alfons, Janes Berthy and Demas Wamaer. 2015. Keragaman Karakter Morfologis dan Agronomis Ubikayu varietas Lokal Maluku. Prosiding seminar nasional sumber daya genetik pertanian 160-168.
    [BibTeX] [Abstract] [PDF: Keragaman Karakter Morfologis dan Agronomis Ubikayu varietas Lokal Maluku ]
    Cassava is the third source of food after rice and maize for the Indonesian people. In addition, as a source of food, cassava can also be used for industrial purposes, such as the food industry a wide range of materials, glue, glucose, fructose, and others. Utilization of cassava germplasm can be done if available morphological and agronomic characters. Characterization of morphological and agronomic properties was conducted in the germplasm collection at KP Makariki, Central Maluku, on January-December 2013. Every accession was planted as many as 40 plants (two lines) through stem cuttings with a spacing of 100 cm x 50 m, plot size of 2 m x 10 m. Fertilization includes 200 kg of urea, 250 kg and 200 kg SP36 KCl/ha, at the time of planting as many as 1/3 parts of urea and KCl as well as all parts of SP36. After 3.5 months of age are given 2/3 parts of urea and KCl. The results showed that the diversity of the qualitative character of 17 local cassava accessions Maluku vary widely, especially color stem, leaf color, and skin color bulbs (outside and inside), only the color of the leaves 17 characters cassava accession no difference (usually green). Quantitative character tuber diameter, tuber length, and number of total planting bulbs have a strong positive correlation with the weight of fresh tubers, otherwise the character petiole length, leaf width, and the number of leaf lobes have a weak positive correlation with the weight of fresh tubers. Fresh tuber weight per plant ranged from 1.20 kg-12.10 kg/plant, an average of 5.25 kg/plant.
    @article{JanesBerthyAlfons15p160,
    title = {{Keragaman Karakter Morfologis dan Agronomis Ubikayu varietas Lokal Maluku}},
    author = {Janes Berthy Alfons and Demas Wamaer},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {160-168},
    year = {2015},
    abstract = {Cassava is the third source of food after rice and maize for the Indonesian people.
    In addition, as a source of food, cassava can also be used for industrial purposes,
    such as the food industry a wide range of materials, glue, glucose, fructose, and
    others. Utilization of cassava germplasm can be done if available morphological
    and agronomic characters. Characterization of morphological and agronomic
    properties was conducted in the germplasm collection at KP Makariki, Central
    Maluku, on January-December 2013. Every accession was planted as many as 40
    plants (two lines) through stem cuttings with a spacing of 100 cm x 50 m, plot size
    of 2 m x 10 m. Fertilization includes 200 kg of urea, 250 kg and 200 kg SP36
    KCl/ha, at the time of planting as many as 1/3 parts of urea and KCl as well as all
    parts of SP36. After 3.5 months of age are given 2/3 parts of urea and KCl. The
    results showed that the diversity of the qualitative character of 17 local cassava
    accessions Maluku vary widely, especially color stem, leaf color, and skin color
    bulbs (outside and inside), only the color of the leaves 17 characters cassava
    accession no difference (usually green). Quantitative character tuber diameter,
    tuber length, and number of total planting bulbs have a strong positive correlation
    with the weight of fresh tubers, otherwise the character petiole length, leaf width,
    and the number of leaf lobes have a weak positive correlation with the weight of fresh tubers. Fresh tuber weight per plant ranged from 1.20 kg-12.10 kg/plant, an
    average of 5.25 kg/plant.},
    keywords = {Cassava, morphological, agronomic, characterization},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/19-Janes Berthy Alfons - BPTP Maluku.pdf}
    }
  20. Cahyaningrum, Hermawati and Yayat Hidayat. 2015. Eksplorasi dan Karakterisasi Plasma Nutfah Kacang Tanah di Maluku Utara. Prosiding seminar nasional sumber daya genetik pertanian 169-175.
    [BibTeX] [Abstract] [PDF: Eksplorasi dan Karakterisasi Plasma Nutfah Kacang Tanah di Maluku Utara ]
    In an effort to develop a superior varieties, germplasm availability in sufficient number and diversity of its character become main capital. Collection and characterization of groundnut germplasm is an important part that should be considered properly. Identification and exploration of groundnut germplasm accessions locally in North Maluku was conduted at three regions in North Maluku. Groundnut multiplication was conducted at farmers’ fields in Kao Teluk sub distrik, North Halmahera district, North Maluku province on March to November 2013, by using a participatory approach involving farmer cooperators. The aim of this activity was to obtain important agronomist informations about the local groundnut in North Maluku. The study showed there were four groundnut accessions, consist of 3 groundnut accessions were red seed and 1 groundnut accession was white seed. The results of the study showed local red seed groundut has harvest time on 95 after sowing, plant height 62 cm, shoot weight 393 g, 100 seed weight 39-40 grams, the average of pods 18-21 pcs/plant, and the number of seeds 2 seeds/pods, with productivity between 2.8 to 3.2 tons/ha.
    @article{HermawatiCahyaningrum15p169,
    title = {{Eksplorasi dan Karakterisasi Plasma Nutfah Kacang Tanah di Maluku Utara}},
    author = {Hermawati Cahyaningrum and Yayat Hidayat},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {169-175},
    year = {2015},
    abstract = {In an effort to develop a superior varieties, germplasm availability in sufficient number and diversity of its character become main capital. Collection and characterization of groundnut germplasm is an important part that should be considered properly. Identification and exploration of groundnut germplasm accessions locally in North Maluku was conduted at three regions in North Maluku. Groundnut multiplication was conducted at farmers' fields in Kao Teluk sub distrik, North Halmahera district, North Maluku province on March to November 2013, by using a participatory approach involving farmer cooperators. The aim of this activity was to obtain important agronomist informations about the local groundnut in North Maluku. The study showed there were four groundnut accessions, consist of 3 groundnut accessions were red seed and 1 groundnut accession was white seed. The results of the study showed local red seed groundut has harvest time on 95 after sowing, plant height 62 cm, shoot weight 393 g, 100 seed weight 39-40 grams, the average of pods 18-21 pcs/plant, and the number of seeds 2 seeds/pods, with productivity between 2.8 to 3.2 tons/ha.},
    keywords = {germplasm, groundnut, north maluku},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/20-Hermawati Cahyaningrum - BPTP Maluku Utara.pdf}
    }
  21. Ahmad, Aisyah and Nanang Buri. 2015. Karakterisasi Sagu Lokal di Kabupaten Bone Bolango Provinsi Gorontalo. Prosiding seminar nasional sumber daya genetik pertanian 176-183.
    [BibTeX] [Abstract] [PDF: Karakterisasi Sagu Lokal di Kabupaten Bone Bolango Provinsi Gorontalo ]
    The purpose of this study was to obtain information about the type and characteristics of potentialy sago palm in Bone Bolango regency for suatainable management and utilization of sago. Exploration was conducted in Duano village in the subdistrict of Central Suwawa and Huloduatamo village in the sub-district of Suwawa in Bone Bolango district during March 2014. Research was conducted by using descriptive method, using purposive sampling. Plant samples used in this study were productive trees (ready to harvest) which were not attacked by pests and diseases. From this research, three accessions of sago were obtaibed i.e. Tutu Tumba, Tumba Ale and Tumba Duhia. These accessions can be used as a source of clones for propagation of sago plantations to increase productivity.
    @article{AisyahAhmad15p176,
    title = {{Karakterisasi Sagu Lokal di Kabupaten Bone Bolango Provinsi Gorontalo}},
    author = {Aisyah Ahmad and Nanang Buri},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {176-183},
    year = {2015},
    abstract = {The purpose of this study was to obtain information about the type and characteristics of potentialy sago palm in Bone Bolango regency for suatainable management and utilization of sago. Exploration was conducted in Duano village in the subdistrict of Central Suwawa and Huloduatamo village in the sub-district of Suwawa in Bone Bolango district during March 2014. Research was conducted by using descriptive method, using purposive sampling. Plant samples used in this study were productive trees (ready to harvest) which were not attacked by pests and diseases. From this research, three accessions of sago were obtaibed i.e. Tutu Tumba, Tumba Ale and Tumba Duhia. These accessions can be used as a source of clones for propagation of sago plantations to increase productivity.},
    keywords = {sago, accession, characteristics},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/21-Aisyah Ahmad - BPTP Gorontalo.pdf}
    }
  22. Dedi Sugandi Wahyu Wibawa and Afrizon. 2015. Sumber Daya Genetik Tanaman Hortikultura di Provinsi Bengkulu. Prosiding seminar nasional sumber daya genetik pertanian 184-191.
    [BibTeX] [Abstract] [PDF: Sumber Daya Genetik Tanaman Hortikultura di Provinsi Bengkulu ]
    Bengkulu Province has diverse of agro-ecosystem and this indicates richness on genetic resources. Up to now, the data and information related to plant genetic resources (PGR) in Bengkulu has not appropriately managed. This activity aims to: 1). Inventory of horticultural plant genetic resources that possess economic value in Bengkulu and 2). Collect the PGR tor conservation. The activity of inventory carried out by extracting information from various references. This activity resulted in fourteen accessions of horticulture plant that have been and are in the process of release of varieties. A brief description of these accessions presented in this paper.
    @article{andAfrizon15p184,
    title = {{Sumber Daya Genetik Tanaman Hortikultura di Provinsi Bengkulu}},
    author = {Dedi Sugandi, Wahyu Wibawa, and Afrizon},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {184-191},
    year = {2015},
    abstract = {Bengkulu Province has diverse of agro-ecosystem and this indicates richness on genetic resources. Up to now, the data and information related to plant genetic resources (PGR) in Bengkulu has not appropriately managed. This activity aims to: 1). Inventory of horticultural plant genetic resources that possess economic value in Bengkulu and 2). Collect the PGR tor conservation. The activity of inventory carried out by extracting information from various references. This activity resulted in fourteen accessions of horticulture plant that have been and are in the process of release of varieties. A brief description of these accessions presented in this paper.},
    keywords = {bengkulu province, genetic resources, horticulture},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/22-Dedi Sugandi - BPTP Bengkulu.pdf}
    }
  23. Muji Rahayu1 Fitratunnisah and Lestari Ujianto. 2015. Pengelolaan Beberapa Sumber Daya Genetik Tanaman Hortikultura di Pulau Lombok Nusa Tenggara Barat. Prosiding seminar nasional sumber daya genetik pertanian 192-197.
    [BibTeX] [Abstract] [PDF: Pengelolaan Beberapa Sumber Daya Genetik Tanaman Hortikultura di Pulau Lombok Nusa Tenggara Barat ]
    Diversity of plant genetic resources pivotal roles in plant breeding as gene sources to generate new plant type. Lombok Island is reported to have genetic diversity of horticultural crops that have not been much explored, exploited and managed well so feared extinct. The aim of this study is to explore, collection and characterization of some horticultural crops on the island of Lombok West Nusa Tenggara province. The study consisted of several activities, namely exploration on the island of Lombok in three different areas on the plains agroekosistemnya ie low, medium, and high. The results obtain a rare three horticultural crops that is hanging potato plants, bare durian and small chili. These plants are rarely found in other areas.
    @article{andLestariUjianto15p192,
    title = {{Pengelolaan Beberapa Sumber Daya Genetik Tanaman Hortikultura di Pulau Lombok Nusa Tenggara Barat}},
    author = {Muji Rahayu1, Fitratunnisah, and Lestari Ujianto},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {192-197},
    year = {2015},
    abstract = {Diversity of plant genetic resources pivotal roles in plant breeding as gene sources
    to generate new plant type. Lombok Island is reported to have genetic diversity of
    horticultural crops that have not been much explored, exploited and managed well
    so feared extinct. The aim of this study is to explore, collection and
    characterization of some horticultural crops on the island of Lombok West Nusa
    Tenggara province. The study consisted of several activities, namely exploration on
    the island of Lombok in three different areas on the plains agroekosistemnya ie
    low, medium, and high. The results obtain a rare three horticultural crops that is
    hanging potato plants, bare durian and small chili. These plants are rarely found in
    other areas.},
    keywords = {small chili, bare durian, exploration, ex situ, hanging potatoes},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/23-Muji Rahayu - BPTP NTB.pdf}
    }
  24. M. Hidayanto N.R. Ahmadi, Sumarmiyati Fiana Y. and F. R. Abadi. 2015. Karakterisasi Morfologis Durian Lokal Kabupaten Kutai Barat Provinsi Kalimantan Timur. Prosiding seminar nasional sumber daya genetik pertanian 198-207.
    [BibTeX] [Abstract] [PDF: Karakterisasi Morfologis Durian Lokal Kabupaten Kutai Barat Provinsi Kalimantan Timur ]
    Durian is horticulture commodity thatvery popular in Indonesia, with special odor and delicious taste, also has high economic value. Indonesia has high genetic resource, which can be utilized for maintenance material in seeking and constructing new superior variety with better quality and high production level. To understand potency and superiority of genetic resource diversity in East Kalimantan, it was conducted an observation and characterization of morphology of local durian in East Kalimantan starting from March to May 2014. Genetic resources of durian which already characterized were durian Bayan, durian Si Bakul, durian Ketupat, durian Belimbing, durian Buaya, and durian Si Bakul Emas. Durian Bayan has fruit-shape oval, weight of 2.2-2.8 kg, pulp thickness of ± 1.5 cm, sweet taste, production reaches 200-250 for each tree. Durian Si Bakul has fruit shaperounded-oval, pulp thickness of 1.2 cm, nice odor, production around 200-400 of fruits. Durian Ketupat has fruit-shape rounded-oval, weight of 1.7-2.5 kg, pulp thickness of 1.5, with strongest odor, production reaches 200-350 fruits per tree. Durian Belimbing has fruit-shape square rounded like star fruit, golden color, tastes very sweet, odor wasn’t too strong, production 200-400 of fruits per tree. Durian Buaya has fruit-shape round-rectangle, weight of 2.5-3.5 kg, pulp tastes sweet, nice aroma, production 250-450 of each tree. Durian Si Bakul Emas has fruit-shape rounded, weight of 2-2.7 kg, rind thickness of 0.8-1.1 cm, sweet taste, nice aroma of pulp, production of 200-400 fruit per tree.
    @article{Abadi15p198,
    title = {{Karakterisasi Morfologis Durian Lokal Kabupaten Kutai Barat Provinsi Kalimantan Timur}},
    author = {M. Hidayanto, N.R. Ahmadi, Sumarmiyati, Y. Fiana, and F.R. Abadi},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {198-207},
    year = {2015},
    abstract = {Durian is horticulture commodity thatvery popular in Indonesia, with special odor and delicious taste, also has high economic value. Indonesia has high genetic resource, which can be utilized for maintenance material in seeking and constructing new superior variety with better quality and high production level. To understand potency and superiority of genetic resource diversity in East Kalimantan, it was conducted an observation and characterization of morphology of local durian in East Kalimantan starting from March to May 2014. Genetic resources of durian which already characterized were durian Bayan, durian Si Bakul, durian Ketupat, durian Belimbing, durian Buaya, and durian Si Bakul Emas. Durian Bayan has fruit-shape oval, weight of 2.2-2.8 kg, pulp thickness of ± 1.5 cm, sweet taste, production reaches 200-250 for each tree. Durian Si Bakul has fruit shaperounded-oval, pulp thickness of 1.2 cm, nice odor, production around 200-400 of fruits. Durian Ketupat has fruit-shape rounded-oval, weight of 1.7-2.5 kg, pulp thickness of 1.5, with strongest odor, production reaches 200-350 fruits per tree. Durian Belimbing has fruit-shape square rounded like star fruit, golden color, tastes very sweet, odor wasn't too strong, production 200-400 of fruits per tree. Durian Buaya has fruit-shape round-rectangle, weight of 2.5-3.5 kg, pulp tastes sweet, nice aroma, production 250-450 of each tree. Durian Si Bakul Emas
    has fruit-shape rounded, weight of 2-2.7 kg, rind thickness of 0.8-1.1 cm, sweet
    taste, nice aroma of pulp, production of 200-400 fruit per tree.},
    keywords = {Durian, West Kutai, characterization},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/24-M Hidayanto - BPTP Kalimantan Timur.pdf}
    }
  25. Aidi Noor Rina Dirgahayu Ningsih, Agus Hasbianto and Abdul Sabur. 2015. Sebaran dan Keragaman Plasma Nutfah Mangga di Kalimantan Selatan. Prosiding seminar nasional sumber daya genetik pertanian 208-218.
    [BibTeX] [Abstract] [PDF: Sebaran dan Keragaman Plasma Nutfah Mangga di Kalimantan Selatan ]
    The diversity of agroecosystems and the natural resources available in South Kalimantan led to the genetic diversity of plants. Fruits type of South Kalimantan are the most dominant germplasm than the other. Mango is a fruit crop potential for development because it has a high economic value and has a high level of genetic diversity. Several types of germplasm become vulnerable, endangered and even extinct due to the changes in the use of biological resources and land use change. In an effort to preserve and develop local mango germplasm research needs to be conducted to determine the distribution and diversity of mango in several locations in South Kalimantan, also to find out the status of some kind of mango germplasm to be had in South Kalimantan. To determine the distribution and diversity of mango conducted inventory survey in 5 districts in South Kalimantan (Tanah Laut, Tabalong, Hulu Sungai Selatan, Banjar, Barito Kuala). Each district inventoried existing types of mango by taking a sample of 30 respondents locations. The results showed obtained 19 species/varieties of mangoes of 5 districts, the highest in the district Hulu Sungai Tengah found 11 species/varieties, Banjar 10 species/varieties, Tanah Laut 9 species/varieties, Barito Kuala 8 species/varieties, Tabalong 5 species/varieties. The species/varieties local of mango found doiminant are Kueni (Mangifera odorata) as many as 226 plants (41 accessions), Hampalam (Mangifera sp. L.) 218 plants (77 accessions), Kasturi (Mangfera kasturi) as many as 53 plants (26 accessions), Hambawang (Mangifera foetida) 38 plants (23 accessions). Other types of local mangoes as Asam Putaran, Binjai, Hambawang Putaran, Hampalam Putih, Kacapiur, Panjang, Palipisan, Pipih, and Rawa-rawa rarely found at the study site.
    @article{andAbdulSabur15p208,
    title = {{Sebaran dan Keragaman Plasma Nutfah Mangga di Kalimantan Selatan}},
    author = {Aidi Noor, Rina Dirgahayu Ningsih, Agus Hasbianto, and Abdul Sabur},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {208-218},
    year = {2015},
    abstract = {The diversity of agroecosystems and the natural resources available in South Kalimantan led to the genetic diversity of plants. Fruits type of South Kalimantan are the most dominant germplasm than the other. Mango is a fruit crop potential for development because it has a high economic value and has a high level of genetic diversity. Several types of germplasm become vulnerable, endangered and even extinct due to the changes in the use of biological resources and land use change. In an effort to preserve and develop local mango germplasm research needs to be conducted to determine the distribution and diversity of mango in several locations in South Kalimantan, also to find out the status of some kind of mango germplasm to be had in South Kalimantan. To determine the distribution and diversity of mango conducted inventory survey in 5 districts in South Kalimantan (Tanah Laut, Tabalong, Hulu Sungai Selatan, Banjar, Barito Kuala). Each district inventoried existing types of mango by taking a sample of 30 respondents locations. The results showed obtained 19 species/varieties of mangoes of 5 districts, the highest in the district Hulu Sungai Tengah found 11 species/varieties, Banjar 10 species/varieties, Tanah Laut 9 species/varieties, Barito Kuala 8 species/varieties, Tabalong 5 species/varieties. The species/varieties local of mango found doiminant are Kueni (Mangifera odorata) as many as 226 plants (41 accessions), Hampalam (Mangifera
    sp. L.) 218 plants (77 accessions), Kasturi (Mangfera kasturi) as many as 53 plants
    (26 accessions), Hambawang (Mangifera foetida) 38 plants (23 accessions). Other
    types of local mangoes as Asam Putaran, Binjai, Hambawang Putaran, Hampalam
    Putih, Kacapiur, Panjang, Palipisan, Pipih, and Rawa-rawa rarely found at the
    study site.},
    keywords = {Inventory, dDiversity, mango, germplasm},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/25-Aidi Noor - BPTP Kalimantan Selatan.pdf}
    }
  26. Iskandar Ishaq Basuno and Nandang Sunandar. 2015. Pendugaan daya Hasil Berdasarkan Karakter Morfologis Bunga Rambutan (Nephelium lappaceum L.) dan Kapulasan (Nephelium mutabile Blume). Prosiding seminar nasional sumber daya genetik pertanian 219-227.
    [BibTeX] [Abstract] [PDF: Pendugaan daya Hasil Berdasarkan Karakter Morfologis Bunga Rambutan (Nephelium lappaceum L.) dan Kapulasan (Nephelium mutabile Blume) ]
    The contribution of the Indonesian local fruits to increase farmers’s income and foreign exchange is still limited, although the demand for fresh local fruit is very high. One of the potential important tropical fruit is rambutan (Nephelium lappaceum L.) and kapulasan (Nephelium mutabile Blume). This study aimed to characterize the flower morphology, fruit yield components and fruit production estimates based on quantitative characters of rambutan and kapulasan. Research was done at the Experimental Research Station in Cipaku, Bogor during January- May 2014. Material used in this study were 14 accessions of rambutan and 1 accessions of kapulasan. Regression and correlation analysis were conducted to predict yield as the number of fruits per tree. The results showed that the character length and width of flower bunches cluster was significantly influenced by the amount of fruits. Regression equation Y = -6420.345 + 281.867 X1 + 79.183 X2 can be used to estimate the yield of rambutan and/or of kapulasan as the of number of fruits per tree.
    @article{andNandangSunandar15p219,
    title = {{Pendugaan daya Hasil Berdasarkan Karakter Morfologis Bunga Rambutan (Nephelium lappaceum L.) dan Kapulasan (Nephelium mutabile Blume)}},
    author = {Iskandar Ishaq, Basuno, and Nandang Sunandar},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {219-227},
    year = {2015},
    abstract = {The contribution of the Indonesian local fruits to increase farmers's income and foreign exchange is still limited, although the demand for fresh local fruit is very high. One of the potential important tropical fruit is rambutan (Nephelium lappaceum L.) and kapulasan (Nephelium mutabile Blume). This study aimed to characterize the flower morphology, fruit yield components and fruit production estimates based on quantitative characters of rambutan and kapulasan. Research was done at the Experimental Research Station in Cipaku, Bogor during January- May 2014. Material used in this study were 14 accessions of rambutan and 1 accessions of kapulasan. Regression and correlation analysis were conducted to predict yield as the number of fruits per tree. The results showed that the character length and width of flower bunches cluster was significantly influenced by the amount of fruits. Regression equation Y = -6420.345 + 281.867 X1 + 79.183 X2 can be used to estimate the yield of rambutan and/or of kapulasan as the of number of fruits per tree.},
    keywords = {flower bunches, rambutan, kapulasan, yield, prediction},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/26-Iskandar Ishaq - BPTP Jawa Barat.pdf}
    }
  27. Rembang, Janne H. W. and Joula O. M. Sondakh. 2015. Karakterisasi Pisang Lokal Mas Jarum dan Goroho di Kebun Koleksi Sumberdaya genetik Tanaman Sulawesi Utara. Prosiding seminar nasional sumber daya genetik pertanian 228-235.
    [BibTeX] [Abstract] [PDF: Karakterisasi Pisang Lokal Mas Jarum dan Goroho di Kebun Koleksi Sumberdaya genetik Tanaman Sulawesi Utara ]
    Banana varieties that exist in North Sulawesi has a high level of diversity that has a great opportunity to be developed as farmers economic resource and region.The character differences between varieties can be shown by the appearance of plant performance such as stems, leaves, flowers and fruit. These characteristics can be used as source for plant genetic improvement. The activity was aimed to observe specific characteristics of some local banana from North Sulawesi. The research was conducted at the Genetic Resources Collection of North Sulawesi which located at Pandu Experiment Station from October 2013 to April 2014, by observing some local banana varieties collections focusing on the varieties of bananas “pisang mas jarum” and “goroho” as well as interviews with local farmers for their utilization. Each variety was observed from 5 plants. The banana ‘pisang mas jarum” and ‘goroho” showed different characters interms of plant height and trunk. Banana “goroho” were not too high but had sturdy stems. The banana “pisang mas jarum” leaf character are long and narrow, while “goroho” leaf character are short. Fruit character of banana “goroho” are much larger than that of “pisang mas jarum”.
    @article{Rembang15p228,
    title = {{Karakterisasi Pisang Lokal Mas Jarum dan Goroho di Kebun Koleksi Sumberdaya genetik Tanaman Sulawesi Utara}},
    author = {Janne H.W. Rembang and Joula O.M. Sondakh},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {228-235},
    year = {2015},
    abstract = {Banana varieties that exist in North Sulawesi has a high level of diversity that has a
    great opportunity to be developed as farmers economic resource and region.The
    character differences between varieties can be shown by the appearance of plant
    performance such as stems, leaves, flowers and fruit. These characteristics can be
    used as source for plant genetic improvement. The activity was aimed to observe
    specific characteristics of some local banana from North Sulawesi. The research
    was conducted at the Genetic Resources Collection of North Sulawesi which
    located at Pandu Experiment Station from October 2013 to April 2014, by
    observing some local banana varieties collections focusing on the varieties of
    bananas “pisang mas jarum” and “goroho” as well as interviews with local farmers
    for their utilization. Each variety was observed from 5 plants. The banana ‘pisang
    mas jarum” and ‘goroho” showed different characters interms of plant height and
    trunk. Banana “goroho” were not too high but had sturdy stems. The banana
    “pisang mas jarum” leaf character are long and narrow, while “goroho” leaf
    character are short. Fruit character of banana “goroho” are much larger than that of
    “pisang mas jarum”.},
    keywords = {banana, characterization, varieties},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/27-Janne HW - BPTP Sulawesi Utara.pdf}
    }
  28. Setyorini Widyayanti Kristamtini and Sudarmaji. 2015. KAarakterisasi beberapa Jenis Tanaman Buah Langka di Lahan Pekarangan Daerah Istimewa Yogyakarta. Prosiding seminar nasional sumber daya genetik pertanian 236-247.
    [BibTeX] [Abstract] [PDF: KAarakterisasi beberapa Jenis Tanaman Buah Langka di Lahan Pekarangan Daerah Istimewa Yogyakarta ]
    Yogyakarta Special Region (DIY) has 3.185.80 km 2 areas, divided into four districts (Sleman, Bantul, Kulon Progo, and Gunungkidul) and one city (Yogyakarta). Each district has diverse type ofagroecology. The diversity of area, topography, soil type, and climates has formed genetic resources which are only adapt to a specific environment. One of genetic resources is fruit plants. The aims of the effortwereto conserve, inventerize, and characterize extinction-pronefruit plants in DIY and to know their distribution area. The implementation was carried out by survey mechanism with total number of 150 respondents. The exploration showed that there were 95 fruit accessions which were varied in distribution area. Sixteen fruit accessions have been in extinction-prone condition so that characterization/description to find their potential characters and preservation through in situ and ex situ conservation need to be done.
    @article{andSudarmaji15p236,
    title = {{KAarakterisasi beberapa Jenis Tanaman Buah Langka di Lahan Pekarangan Daerah Istimewa Yogyakarta}},
    author = {Setyorini Widyayanti, Kristamtini, and Sudarmaji},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {236-247},
    year = {2015},
    abstract = {Yogyakarta Special Region (DIY) has 3.185.80 km 2 areas, divided into four districts (Sleman, Bantul, Kulon Progo, and Gunungkidul) and one city (Yogyakarta). Each district has diverse type ofagroecology. The diversity of area, topography, soil type, and climates has formed genetic resources which are only adapt to a specific environment. One of genetic resources is fruit plants. The aims of the effortwereto conserve, inventerize, and characterize extinction-pronefruit plants in DIY and to know their distribution area. The implementation was carried out by survey mechanism with total number of 150 respondents. The exploration showed that there were 95 fruit accessions which were varied in distribution area. Sixteen fruit accessions have been in extinction-prone condition so that characterization/description to find their potential characters and preservation through in situ and ex situ conservation need to be done.},
    keywords = {genetic resources, exploration, characterization, fruit plants, prone},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/28-Setyorini Widyayanti - BPTP Yogyakarta.pdf}
    }
  29. Retno Utami Hatmi Setyorini Widyayanti and Sudarmaji. 2015. Potensi Kepel (Stelechocarpus burahol [Blume] Hook.F & Th.) sebagai Sumber Pangan Fungsional. Prosiding seminar nasional sumber daya genetik pertanian 248-257.
    [BibTeX] [Abstract] [PDF: Potensi Kepel (Stelechocarpus burahol [Blume] Hook.F & Th.) sebagai Sumber Pangan Fungsional ]
    The low economic value and the difficulty of cultivating plants cause scarcity of kepel, that kepel is the identity of DaerahIstimewa Yogyakarta. However, the high value of philosophy and the bioactive compound beneficial to health to be important for conservation. The bioactive compound contained in almost all parts of the plant, ie leaves, flowers, fruit pulp, fruit seeds, rind, and bark. Several bioactive compounds that have been found and researched health benefits are antioxidants, flavonoids, cyclooxigenase-2 inhibitors, anti-hyperuricemic, cytotoxic anti-cancer agents, oral deodorants, and phytoestrogen compounds. This compound has the potential to be developed to be a functional food from kepel.
    @article{andSudarmaji15p248,
    title = {{Potensi Kepel (Stelechocarpus burahol [Blume] Hook.F \& Th.)
    sebagai Sumber Pangan Fungsional}},
    author = {Retno Utami Hatmi, Setyorini Widyayanti, and Sudarmaji},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {248-257},
    year = {2015},
    abstract = {The low economic value and the difficulty of cultivating plants cause scarcity of kepel, that kepel is the identity of DaerahIstimewa Yogyakarta. However, the high value of philosophy and the bioactive compound beneficial to health to be important for conservation. The bioactive compound contained in almost all parts of the plant, ie leaves, flowers, fruit pulp, fruit seeds, rind, and bark. Several bioactive compounds that have been found and researched health benefits are antioxidants, flavonoids, cyclooxigenase-2 inhibitors, anti-hyperuricemic, cytotoxic anti-cancer agents, oral deodorants, and phytoestrogen compounds. This compound has the potential to be developed to be a functional food from kepel.},
    keywords = {kepel, bioactive compound, functional food},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/29-Retno Utami Hatmi - BPTP Yogyakarta.pdf}
    }
  30. Malik, Afrizal and Rohimah Handayani Sri Lestari. 2015. Potensi Tanaman Buah Merah dan Prospek Pengembangannya di Provinsi Papua. Prosiding seminar nasional sumber daya genetik pertanian 258-266.
    [BibTeX] [Abstract] [PDF: Potensi Tanaman Buah Merah dan Prospek Pengembangannya di Provinsi Papua ]
    Red fruit (Pandanus conoideus Lamk.) is a member of the family Pandanaceae which has many uses, e.g. as a medicinal source/raw material for degenerative disorders/diseases such as heart, liver, cholesterol, diabetes, gout, osteoporosis, as well as anti-HIV infection. In addition, pulp juice of red fruit oil can be used as animal feed. Genetic potential of red fruit in its natural habitat is unknown. Paper on the potency of red fruit and its development prospect in Papua aimed to determine its status, distribution and development, genetic preservation aspect, benefit and usefulness, prospect and chance in the future. Many ethnic groups living around Papua affected the naming of each accession of red fruit crops. For while, there were six accessions which were much in demand/cultivated by farmers in Papua, i.e. Melar, Barugum, Ibagaya, Kuanggo, Kanen, and Muni. In general, current processing methods are still developing, simple or traditional, therefore the drug industry and red fruit juice seller reprocess it for more qualified and hygienic product.
    @article{AfrizalMalik15p258,
    title = {{Potensi Tanaman Buah Merah dan Prospek Pengembangannya di Provinsi Papua}},
    author = {Afrizal Malik and Rohimah Handayani Sri Lestari},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {258-266},
    year = {2015},
    abstract = {Red fruit (Pandanus conoideus Lamk.) is a member of the family Pandanaceae which has many uses, e.g. as a medicinal source/raw material for degenerative disorders/diseases such as heart, liver, cholesterol, diabetes, gout, osteoporosis, as well as anti-HIV infection. In addition, pulp juice of red fruit oil can be used as animal feed. Genetic potential of red fruit in its natural habitat is unknown. Paper on the potency of red fruit and its development prospect in Papua aimed to determine its status, distribution and development, genetic preservation aspect, benefit and usefulness, prospect and chance in the future. Many ethnic groups living around Papua affected the naming of each accession of red fruit crops. For while, there were six accessions which were much in demand/cultivated by farmers in Papua, i.e. Melar, Barugum, Ibagaya, Kuanggo, Kanen, and Muni. In general, current processing methods are still developing, simple or traditional, therefore the drug industry and red fruit juice seller reprocess it for more qualified and hygienic product.},
    keywords = {Pandanus conoideus Lamk, potential, development prospects},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/30-Afrizal Malik - BPTP Papua.pdf}
    }
  31. Ikrarwati Yudi Sastro, Tezar Ramdhan and Nofi Anisatun Rokhmah. 2015. Karakterisasi Morfologis dan Potensi Ekonomi Sukun (Artocarpus altilis Fobs) Lahan Salin di Kepulauan Seribu. Prosiding seminar nasional sumber daya genetik pertanian 267-273.
    [BibTeX] [Abstract] [PDF: Karakterisasi Morfologis dan Potensi Ekonomi Sukun (Artocarpus altilis Fobs) Lahan Salin di Kepulauan Seribu ]
    Characterization of breadfruit (Artocarpus altilis Fobs.) was conducted using survey method during May-June 2014 in six islands of Kepulauan Seribu, Jakarta, i.e. Tidung Kecil, Tidung Besar, Payung, Pramuka, Panggang, and Karya. The study was aimed to determine the specific characteristics and economic potential of breadfruit in Kepulauan Seribu. As the result, two types of breadfruit had been obtained. There weremorphological differences with the main distinguished characters in the fruit and leave morphology. Breadfruit type 1 hadfruits with some characteristic: oval shape, flat and smooth rind surface (did not have thorns), and yellowish flesh. Whereas, the fruits of breadfruit type 2 had egg-shaped, prickly and rough rind surface, and white flesh. Meanwhile, the leaves of breadfuit type 1 had deep notchs and tapered leaves base with fine hairs on the leaves bone. On the other hand, the leaves of breadfruit type 2 had more shallow notchs without fine hairs on the leaves bone. An economic potential of breadfruit was high enough, due to Kepulauan Seribu have a high tourism potential too. In this case, breadfruits with available continously can be sold to the tourist in the form of processed foods.
    @article{andNofiAnisatunRokhmah15p267,
    title = {{Karakterisasi Morfologis dan Potensi Ekonomi Sukun (Artocarpus altilis Fobs) Lahan Salin di Kepulauan Seribu}},
    author = {Ikrarwati, Yudi Sastro, Tezar Ramdhan, and Nofi Anisatun Rokhmah},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {267-273},
    year = {2015},
    abstract = {Characterization of breadfruit (Artocarpus altilis Fobs.) was conducted using survey method during May-June 2014 in six islands of Kepulauan Seribu, Jakarta, i.e. Tidung Kecil, Tidung Besar, Payung, Pramuka, Panggang, and Karya. The study was aimed to determine the specific characteristics and economic potential of breadfruit in Kepulauan Seribu. As the result, two types of breadfruit had been obtained. There weremorphological differences with the main distinguished characters in the fruit and leave morphology. Breadfruit type 1 hadfruits with some characteristic: oval shape, flat and smooth rind surface (did not have thorns), and yellowish flesh. Whereas, the fruits of breadfruit type 2 had egg-shaped, prickly and rough rind surface, and white flesh. Meanwhile, the leaves of breadfuit type 1 had deep notchs and tapered leaves base with fine hairs on the leaves bone. On the other hand, the leaves of breadfruit type 2 had more shallow notchs without fine hairs on the leaves bone. An economic potential of breadfruit was high enough, due to Kepulauan Seribu have a high tourism potential too. In this case, breadfruits with available continously can be sold to the tourist in the form of processed foods.},
    keywords = {breadfruits, kepulauan seribu, characterization},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/31-Ikrarwati - BPTP DKI Jakarta.pdf}
    }
  32. Susilawati Suparman, Agustini Harmini Sinta Purwandari Adrial S. . . E. and R. Massiaoi. 2015. Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah. Prosiding seminar nasional sumber daya genetik pertanian 274-286.
    [BibTeX] [Abstract] [PDF: Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah ]
    Inventory of Agriculture Genetic Resources of Central Kalimantan. Central Kalimantan has a fairly wide area coverage with high biodiversity that can be used to support food security. Unfortunately, there are less attention for the plant genetic resources. The objectives of this study are to conduct an inventory of genetic resources in yards and gardens and to compile a database of genetic resources in Central Kalimantan. The activities were carried out in few steps from January to December 2013 in all districts/cities with agro-ecosystem of tidal wetlands, swampy, peat, wet climate of dry land and irrigated land. Method of activity is a survey in all districts in Central Kalimantan. Site selection is purposive sampling, by choosing 30 yards and 5 non-yard field. In each district selected three sub- districts and in each sub-district selected three villages. At each village selected 3-4 yards with genetic resources, so total of 30 yards were collected. Data collected in the form of passport data and other supporting data. Results obtained from phase I in six districts showed that the plant genetic resources in the yards are very diverse and the level of diversity is higher than the non-yard land. There are as many as 321 types of genetic resources, comprised of 80 types of fruit crops, 57 types of food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15 estate crops, and 9 livestock.
    @article{Massiaoi15p274,
    title = {{Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah}},
    author = {Susilawati, Suparman, S. Agustini, Harmini, Sinta E. Purwandari, Adrial, and R. Massiaoi},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {274-286},
    year = {2015},
    abstract = {Inventory of Agriculture Genetic Resources of Central Kalimantan. Central Kalimantan has a fairly wide area coverage with high biodiversity that can be used to support food security. Unfortunately, there are less attention for the plant genetic resources. The objectives of this study are to conduct an inventory of genetic resources in yards and gardens and to compile a database of genetic resources in Central Kalimantan. The activities were carried out in few steps from January to December 2013 in all districts/cities with agro-ecosystem of tidal wetlands, swampy, peat, wet climate of dry land and irrigated land. Method of activity is a survey in all districts in Central Kalimantan. Site selection is purposive sampling, by choosing 30 yards and 5 non-yard field. In each district selected three sub- districts and in each sub-district selected three villages. At each village selected 3-4 yards with genetic resources, so total of 30 yards were collected. Data collected in the form of passport data and other supporting data. Results obtained from phase I in six districts showed that the plant genetic resources in the yards are very diverse and the level of diversity is higher than the non-yard land. There are as many as 321 types of genetic resources, comprised of 80 types of fruit crops, 57 types of food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15
    estate crops, and 9 livestock.},
    keywords = {Inventory, diversity, genetic resources, Central Kalimantan},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/32-Susilawati - BPTP Kalimantan Tengah.pdf}
    }
  33. Muji Rahayu Fitrahtunnisah, Sujudi and Gede Marta. 2015. Potensi Sumber Daya genetik Tanaman Lokal Bawang Putih di Kabupaten Lombok Timur, Provinsi Nusa Tenggara Barat. Prosiding seminar nasional sumber daya genetik pertanian 287-292.
    [BibTeX] [Abstract] [PDF: Potensi Sumber Daya genetik Tanaman Lokal Bawang Putih di Kabupaten Lombok Timur, Provinsi Nusa Tenggara Barat ]
    Research on the potential of plant genetic resources of local garlic in East Lombok district had been done in two years period (from 2013 to 2014). The first activities was done to identify plant genetic resources of four types-local garlic i.e.; Sangga, Bagong, accession A and accession B. The four types of garlic were planted in 2014 at farmers’ fields to provide the vegetative and yield characterization. Of these studies showed that the local garlic “Sangga” had the best character and yield preferred by farmers because of its high yield on both normal tubers yields and “Nunggal” tuber. When compared with others type of plant characters, the “Bagong” type of plant garlic seem to have an interesting bulbs character due to its larger size, although the plants harvesting had one month.much longer.
    @article{andGedeMarta15p287,
    title = {{Potensi Sumber Daya genetik Tanaman Lokal Bawang Putih di Kabupaten Lombok Timur, Provinsi Nusa Tenggara Barat}},
    author = {Muji Rahayu, Fitrahtunnisah, Sujudi, and Gede Marta},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {287-292},
    year = {2015},
    abstract = {Research on the potential of plant genetic resources of local garlic in East Lombok
    district had been done in two years period (from 2013 to 2014). The first activities
    was done to identify plant genetic resources of four types-local garlic i.e.; Sangga,
    Bagong, accession A and accession B. The four types of garlic were planted in
    2014 at farmers' fields to provide the vegetative and yield characterization. Of
    these studies showed that the local garlic “Sangga” had the best character and yield
    preferred by farmers because of its high yield on both normal tubers yields and
    “Nunggal” tuber. When compared with others type of plant characters, the
    “Bagong” type of plant garlic seem to have an interesting bulbs character due to its
    larger size, although the plants harvesting had one month.much longer.},
    keywords = {genetic resources, local plants, garlic, character},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/33-Muji Rahayu - BPTP NTB.pdf}
    }
  34. Yayat Hidayat* Andriko Noto Susanto, Wawan S. and Miskat Ramdhani. 2015. Keragaan Fisik dan Morfologis Bawang Merah Topo Maluku Utara. Prosiding seminar nasional sumber daya genetik pertanian 293-298.
    [BibTeX] [Abstract] [PDF: Keragaan Fisik dan Morfologis Bawang Merah Topo Maluku Utara ]
    Topo shallot onion is a local varieties that have specific characteristics. a Topo onion is generally cultivated conventionally plain medium without technology introduction. Planting of Topo onions in lowland with the introduction of technology may be able to change the morphological characteristics. The purpose of this study was to determine Topo onion in physically and morphologically in lowland. The experiment was conducted from January to May 2014 in the centre of Tidore Islands. This research used the survey method which is the location determined by purposive in their hometown Topo onions in the Tidore island and field laboratory in AIAT North Maluku garden collection (1-5 m dpl). Based on the study on Topo onion resulted some characteristics : (1) solid layer of bulbs, (2) oval tuber shape, (3) cloves/bulbs do not overlap, (4) color dull bulbs, (5) small tubers with a mean diameter 1.37 cm, (6) the average tuber weight 2.4 grams, and (7) the sharp aroma. The results of the study showed changing on morphological characters Topo onions, such as the difference in the weight and diameter of the bulb Topo onion. Topo Onion in field laboratory is bigger than the Topo onions originating from the island of Tidore. Topo Onion has average of tuber weight 3.13 grams and a diameter 1.49 cm.
    @article{andMiskatRamdhani15p293,
    title = {{Keragaan Fisik dan Morfologis Bawang Merah Topo Maluku Utara}},
    author = {Yayat Hidayat*, Andriko Noto Susanto, Wawan S., and Miskat Ramdhani},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {293-298},
    year = {2015},
    abstract = {Topo shallot onion is a local varieties that have specific characteristics. a Topo onion is generally cultivated conventionally plain medium without technology introduction. Planting of Topo onions in lowland with the introduction of technology may be able to change the morphological characteristics. The purpose of this study was to determine Topo onion in physically and morphologically in lowland. The experiment was conducted from January to May 2014 in the centre of Tidore Islands. This research used the survey method which is the location determined by purposive in their hometown Topo onions in the Tidore island and field laboratory in AIAT North Maluku garden collection (1-5 m dpl). Based on the study on Topo onion resulted some characteristics : (1) solid layer of bulbs, (2) oval tuber shape, (3) cloves/bulbs do not overlap, (4) color dull bulbs, (5) small tubers with a mean diameter 1.37 cm, (6) the average tuber weight 2.4 grams, and (7) the sharp aroma. The results of the study showed changing on morphological characters Topo onions, such as the difference in the weight and diameter of the bulb Topo onion. Topo Onion in field laboratory is bigger than the Topo onions originating from the island of Tidore. Topo Onion has average of tuber weight 3.13 grams and a diameter 1.49 cm.},
    keywords = {topo onion, morphology},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/34-Yayat Hidayat - BPTP Maluku Utara.pdf}
    }
  35. Rr. Ernawati Firdausil Akhyar Ben, Junita Barus Andarias Danarsih M. M. and Muhamad Rhomdan. 2015. Inventarisasi Sumber Daya genetik di Provinsi Lampung. Prosiding seminar nasional sumber daya genetik pertanian 299-306.
    [BibTeX] [Abstract] [PDF: Inventarisasi Sumber Daya genetik di Provinsi Lampung ]
    Lampung Province is quite wide area with the diversity of genetic resources spread in fourteen districts. This research aimed to do inventory activity of genetic resources in Lampung Province. It was implemented in one district, Pringsewu, consisted of eight sub-districts, 126 villages, and 5 countries. Sub-district capital is Pringsewu. Inventory activity of genetic resources was done by collecting data from thirty home-yard samples. The inventory results showed a variety of genetic resources in home-yard which was similar at all sub-district of Pringsewu, i.e. food, horticultural, estate, and medicinal crops. An effort to conserve the genetic resources has been made by collecting the data from collection station owned by government and individuals.
    @article{andMuhamadRhomdan15p299,
    title = {{Inventarisasi Sumber Daya genetik di Provinsi Lampung}},
    author = {Rr. Ernawati, Firdausil Akhyar Ben, Junita Barus, Andarias M.M., Danarsih, and
    Muhamad Rhomdan},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {299-306},
    year = {2015},
    abstract = {Lampung Province is quite wide area with the diversity of genetic resources spread in fourteen districts. This research aimed to do inventory activity of genetic resources in Lampung Province. It was implemented in one district, Pringsewu, consisted of eight sub-districts, 126 villages, and 5 countries. Sub-district capital is Pringsewu. Inventory activity of genetic resources was done by collecting data from thirty home-yard samples. The inventory results showed a variety of genetic resources in home-yard which was similar at all sub-district of Pringsewu, i.e. food, horticultural, estate, and medicinal crops. An effort to conserve the genetic resources has been made by collecting the data from collection station owned by government and individuals.},
    keywords = {diversity, genetic resources, lampung province},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/35-Ernawati - BPTP Lampung.pdf}
    }
  36. Muji Rahayu Fitrahtunnisa, Achmad Suriadi Baiq Tri Ratna Erawati and Nani Herawati. 2015. Keragaan Inventarisasi dan Pengelolaan Sumber Daya genetik di Pulau Lombok Provinsi Nusa Tenggara Barat. Prosiding seminar nasional sumber daya genetik pertanian 307-316.
    [BibTeX] [Abstract] [PDF: Keragaan Inventarisasi dan Pengelolaan Sumber Daya genetik di Pulau Lombok Provinsi Nusa Tenggara Barat ]
    West Nusa Tenggara Province is an archipelagos province that forming diversity of the land and ocean natural ecosystems, so it has high diversity of genetic resources. Unfortunately, depreciation of local genetic resources keeps happening and over again. In an effort to maintain genetic resources sustainability and food sufficiency, plant genetic resources (PGR) management should be done in a sustainable way. In 2013, West Nusa Tenggara Assessment Institute for Agricultural Technology (AIAT/BPTP) implemented PGR inventory and management in Lombok Island. This research aimed to know the diversity, benefits, and management identification of PGR in Lombok Island. Based on the inventory results, there were several local PGR: 14 local accessions of durian (2 endangered: durian without spines and durian without partition), 14 local accessions of mango (2 endangered: Derakanda and Bapang mangoes), 5 local accessions of a citrus (3 endangered: Kota Raja pomelo, Barejulat citrus, and Sempaga citrus), 2 local accessions of Ipomoea aquatic (Gomong and Aini), 12 accessions of banana (4 endangered), 8 local accessions of rice, various of drought-resistant komak and gude beans, local maize, and others. Among the diversity of PGR, it seemed that some types of bean crops were specifically found at an extreme dry land in Lombok Island that could be used as sources for food and feed. These plant groups have potential to be developed in national food security program. Meanwhile, several endangered horticultural and rice accessions could be used in plant varieties improvement.
    @article{andNaniHerawati15p307,
    title = {{Keragaan Inventarisasi dan Pengelolaan Sumber Daya genetik di Pulau Lombok Provinsi Nusa Tenggara Barat}},
    author = {Muji Rahayu, Fitrahtunnisa, Achmad Suriadi, Baiq Tri Ratna Erawati, and Nani Herawati},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {307-316},
    year = {2015},
    abstract = {West Nusa Tenggara Province is an archipelagos province that forming diversity of the land and ocean natural ecosystems, so it has high diversity of genetic resources. Unfortunately, depreciation of local genetic resources keeps happening and over again. In an effort to maintain genetic resources sustainability and food sufficiency, plant genetic resources (PGR) management should be done in a sustainable way. In 2013, West Nusa Tenggara Assessment Institute for Agricultural Technology (AIAT/BPTP) implemented PGR inventory and management in Lombok Island. This research aimed to know the diversity, benefits, and management identification of PGR in Lombok Island. Based on the inventory results, there were several local PGR: 14 local accessions of durian (2 endangered: durian without spines and durian without partition), 14 local accessions of mango (2 endangered: Derakanda and Bapang mangoes), 5 local accessions of a citrus (3 endangered: Kota Raja pomelo, Barejulat citrus, and Sempaga citrus), 2 local accessions of Ipomoea aquatic (Gomong and Aini), 12 accessions of banana (4 endangered), 8 local accessions of rice, various of drought-resistant komak and gude beans, local maize, and others. Among the diversity of PGR, it seemed that some types of bean crops were specifically found at an extreme dry land in Lombok Island that could be used
    as sources for food and feed. These plant groups have potential to be developed in
    national food security program. Meanwhile, several endangered horticultural and
    rice accessions could be used in plant varieties improvement.},
    keywords = {Local GR of West Nusa Tenggara, collection, ex situ},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/36-Muji Rahayu - BPTP NTB.pdf}
    }
  37. E.Y. Hosang A. Bire, Sendow Doga Menge C. B. . H. L. . D. and C. Hanggongu. 2015. Keragaan Sumber Daya Genetik Tanaman di Timor Barat, Provinsi Nusa Tenggara Timur, serta Strategi Pengelolaannya. Prosiding seminar nasional sumber daya genetik pertanian 317-325.
    [BibTeX] [Abstract] [PDF: Keragaan Sumber Daya Genetik Tanaman di Timor Barat, Provinsi Nusa Tenggara Timur, serta Strategi Pengelolaannya ]
    Local genetic resources are materials those can be used directly for food or as genetic sources in the development of superior varieties. Local genetic resources of West Timor, Nusa Tenggara Timur (NTT) Province, has potential to be developed as extreme drought tolerant varieties or can also be used as genetic sources in the development of specific location superior varieties. The research was aimed to know the genetic diversity of food and horticultural crops of West Timor, NTT Province, and to develop ex situ conservation of food crops those are considered as endangered species. Research was done with the survey and collection methods of local genetic resources. The determination of 30 samples was done by cluster random sampling in five districts of West Timor. Ex situ conservation of endangered commodities was done by planting them in collection garden. Based on inventory in West Timor, horticultural crops had higher variation, while food crop variations were low. It happened because West Timor farmers planted superior varieties whose production yields were higher than local accessions. This change strengthen the need to do the inventory and conservation of local accessions. Variation of accessions planted in the yard was higher than those planted on farm. Distribution of species found mostly in fruit crops and Kabupaten Timor Tengah Selatan (TTS) was the district with the largest number of species in West Timor.
    @article{Hanggongu15p317,
    title = {{Keragaan Sumber Daya Genetik Tanaman di Timor Barat, Provinsi Nusa Tenggara Timur, serta Strategi Pengelolaannya}},
    author = {E.Y. Hosang, A. Bire, C.B. Sendow, H.L. Doga, D. Menge, and C. Hanggongu},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {317-325},
    year = {2015},
    abstract = {Local genetic resources are materials those can be used directly for food or as genetic sources in the development of superior varieties. Local genetic resources of West Timor, Nusa Tenggara Timur (NTT) Province, has potential to be developed as extreme drought tolerant varieties or can also be used as genetic sources in the development of specific location superior varieties. The research was aimed to know the genetic diversity of food and horticultural crops of West Timor, NTT Province, and to develop ex situ conservation of food crops those are considered as endangered species. Research was done with the survey and collection methods of local genetic resources. The determination of 30 samples was done by cluster random sampling in five districts of West Timor. Ex situ conservation of endangered commodities was done by planting them in collection garden. Based on inventory in West Timor, horticultural crops had higher variation, while food crop variations were low. It happened because West Timor farmers planted superior varieties whose production yields were higher than local accessions. This change strengthen the need to do the inventory and conservation of local accessions. Variation of accessions planted in the yard was higher than those planted on farm. Distribution of species found mostly in fruit crops and Kabupaten Timor Tengah
    Selatan (TTS) was the district with the largest number of species in West Timor.},
    keywords = {West Timor, diversity, genetic resources},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/37-Hosang - BPTP NTT.pdf}
    }
  38. Susilawati Suparman, Agustini Harmini Sinta Purwandari Adrial S. . . E. and R. Massiaoi. 2015. Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah. Prosiding seminar nasional sumber daya genetik pertanian 326-338.
    [BibTeX] [Abstract] [PDF: Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah ]
    Inventory of Agriculture Genetic Resources of Central Kalimantan. Central Kalimantan has a fairly wide area coverage with high biodiversity that can be used to support food security. Unfortunately, there are less attention for the plant genetic resources. The objectives of this study are to conduct an inventory of genetic resources in yards and gardens and to compile a database of genetic resources in Central Kalimantan. The activities were carried out in few steps from January to December 2013 in all districts/cities with agro-ecosystem of tidal wetlands, swampy, peat, wet climate of dry land and irrigated land. Method of activity is a survey in all districts in Central Kalimantan. Site selection is purposive sampling, by choosing 30 yards and 5 non-yard field. In each district selected three sub- districts and in each sub-district selected three villages. At each village selected 3-4 yards with genetic resources, so total of 30 yards were collected. Data collected in the form of passport data and other supporting data. Results obtained from phase I in six districts showed that the plant genetic resources in the yards are very diverse and the level of diversity is higher than the non-yard land. There are as many as 321 types of genetic resources, comprised of 80 types of fruit crops, 57 types of food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15 estate crops, and 9 livestock.food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15 estate crops, and 9 livestock.
    @article{Massiaoi15p326,
    title = {{Inventarisasi Sumber Daya Genetik Pertanian Kalimantan Tengah}},
    author = {Susilawati, Suparman, S. Agustini, Harmini, Sinta E Purwandari, Adrial, and R. Massiaoi},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {326-338},
    year = {2015},
    abstract = {Inventory of Agriculture Genetic Resources of Central Kalimantan. Central Kalimantan has a fairly wide area coverage with high biodiversity that can be used to support food security. Unfortunately, there are less attention for the plant genetic resources. The objectives of this study are to conduct an inventory of genetic resources in yards and gardens and to compile a database of genetic resources in Central Kalimantan. The activities were carried out in few steps from January to December 2013 in all districts/cities with agro-ecosystem of tidal wetlands, swampy, peat, wet climate of dry land and irrigated land. Method of activity is a survey in all districts in Central Kalimantan. Site selection is purposive sampling, by choosing 30 yards and 5 non-yard field. In each district selected three sub- districts and in each sub-district selected three villages. At each village selected 3-4 yards with genetic resources, so total of 30 yards were collected. Data collected in the form of passport data and other supporting data. Results obtained from phase I in six districts showed that the plant genetic resources in the yards are very diverse and the level of diversity is higher than the non-yard land. There are as many as 321 types of genetic resources, comprised of 80 types of fruit crops, 57 types of food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15
    estate crops, and 9 livestock.food crops, 72 spices, 33ornamental plants, 55 of horticulture/vegetable crops, 15
    estate crops, and 9 livestock.},
    keywords = {Inventory, diversity, genetic resources, Central Kalimantan},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/38-Susilawati - BPTP Kalimantan Tengah.pdf}
    }
  39. Nirmala Friyanti Devy Hardiyanto and Aryawaita. 2015. Status dan Pola Penyebaran Sumber Daya Genetik Tanaman Pekarangan Rumah di Sumatera Barat. Prosiding seminar nasional sumber daya genetik pertanian 339-351.
    [BibTeX] [Abstract] [PDF: Status dan Pola Penyebaran Sumber Daya Genetik Tanaman Pekarangan Rumah di Sumatera Barat ]
    Recently, many households have developed cultivated several crops in their home gardens that contribute either to food security or as plant genetic conservation. However, inventarization and distribution of plant genetic resources have been not yet documented especially in West Sumatera. Therefore, research on the status and distribution of plant genetic resources in home gardens is required. The aim of this research was to inventory and to evaluate the distribution pattern of plant genetic resources. This research was carried out from January to October 2013 in three districts i.e; Agam, Solok dan Pariaman, West Sumatera with 56 species of ornamental plants, followed by fruits (30 species), spices (27 species), and vegetables (23 species). High distribution pattern of ornamental were found in district of Agam (76%) and Solok (73%). The distribution pattern of fruits, vegetables, and medicinal plant dominantly grown in Pariaman districtwere 90%, 74%, and 81%, respectively. Pariaman district produced the highest diversity index which was catagorized as a medium level, whereas; Agam had the lowest (H < 1). Solok district showed the highest uniformly index (EH = 0.998). The higher similarity value of species structure (0.63–0.64) were dominated by food crops (casava, sweet potato), and biopharmaca (ginger, turmaric, calladium).
    @article{andAryawaita15p339,
    title = {{Status dan Pola Penyebaran Sumber Daya Genetik Tanaman Pekarangan Rumah di Sumatera Barat}},
    author = {Nirmala Friyanti Devy, Hardiyanto, and Aryawaita},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {339-351},
    year = {2015},
    abstract = {Recently, many households have developed cultivated several crops in their home gardens that contribute either to food security or as plant genetic conservation. However, inventarization and distribution of plant genetic resources have been not yet documented especially in West Sumatera. Therefore, research on the status and distribution of plant genetic resources in home gardens is required. The aim of this research was to inventory and to evaluate the distribution pattern of plant genetic resources. This research was carried out from January to October 2013 in three districts i.e; Agam, Solok dan Pariaman, West Sumatera with 56 species of ornamental plants, followed by fruits (30 species), spices (27 species), and vegetables (23 species). High distribution pattern of ornamental were found in district of Agam (76%) and Solok (73%). The distribution pattern of fruits, vegetables, and medicinal plant dominantly grown in Pariaman districtwere 90%, 74%, and 81%, respectively. Pariaman district produced the highest diversity index which was catagorized as a medium level, whereas; Agam had the lowest (H < 1). Solok district showed the highest uniformly index (EH = 0.998). The higher similarity value of species structure (0.63–0.64) were dominated by food crops
    (casava, sweet potato), and biopharmaca (ginger, turmaric, calladium).},
    keywords = {Home graden, index Shannon, distribution pattern, plant genetic
    resources},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/39-Nirmala Friyanti Devy - BPTP Sumatera Barat.pdf}
    }
  40. Afrizon Dedi Sugandi and Siti Rosmanah. 2015. Keanekaragaman Sumber Daya Genetik Tanaman pada Lahan Dataran Rendah di Provinsi Bengkulu. Prosiding seminar nasional sumber daya genetik pertanian 352-362.
    [BibTeX] [Abstract] [PDF: Keanekaragaman Sumber Daya Genetik Tanaman pada Lahan Dataran Rendah di Provinsi Bengkulu ]
    Diversity assessment of plant genetic resources (PGR) in lowland of Bengkulu is implemented in 5 districts namely North Bengkulu, Seluma, South Bengkulu, Bengkulu Kaur and Central. These areas are considered represent the low-lying areas because they are in the height range between 0-400 m above sea level. The purpose of this study was to determine the genetic resources diversity of agricultural crops in the lowlands province of Bengkulu. Survey were conducted from February to May 2014, by survey on 30 points in each district selected by means of an inventory of the type of crops, fruit crops, plantation crops, medicinal plants, ornamental plants and vegetable crops. Selection of yards sample is based on distribution and number of plants owned collection considered as representative of PGR in the district/town. The data was taken i.e. type of plants, number of utilization. The data were then tabulated and analyzed to see diversity using PRG diversity index in a region using the Shannon Index. The results showed that for in varias in lowland Bengkulu was very diverse. The ornamental and estate crops showed high diversity compared to others. These survey have already identified 20 of food crops, 97 of fruit plants, 63 of estate crops, 36 of medicinal plants, 67 of ornamental plants and 34 of vegetables.
    @article{andSitiRosmanah15p352,
    title = {{Keanekaragaman Sumber Daya Genetik Tanaman pada Lahan Dataran Rendah di Provinsi Bengkulu}},
    author = {Afrizon, Dedi Sugandi, and Siti Rosmanah},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {352-362},
    year = {2015},
    abstract = {Diversity assessment of plant genetic resources (PGR) in lowland of Bengkulu is implemented in 5 districts namely North Bengkulu, Seluma, South Bengkulu, Bengkulu Kaur and Central. These areas are considered represent the low-lying areas because they are in the height range between 0-400 m above sea level. The purpose of this study was to determine the genetic resources diversity of agricultural crops in the lowlands province of Bengkulu. Survey were conducted from February to May 2014, by survey on 30 points in each district selected by means of an inventory of the type of crops, fruit crops, plantation crops, medicinal plants, ornamental plants and vegetable crops. Selection of yards sample is based on distribution and number of plants owned collection considered as representative of PGR in the district/town. The data was taken i.e. type of plants, number of utilization. The data were then tabulated and analyzed to see diversity using PRG diversity index in a region using the Shannon Index. The results showed that for in varias in lowland Bengkulu was very diverse. The ornamental and estate crops showed high diversity compared to others. These survey have already identified 20 of food crops, 97 of fruit plants, 63 of estate crops, 36 of medicinal plants, 67 of ornamental plants and 34 of vegetables.},
    keywords = {diversity, prg, lowland},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/40-Afrizon - BPTP Bengkulu.pdf}
    }
  41. Maintang Fadjry Djufry and Sahardi. 2015. Keragaan dan Pemanfaatan Sumber Daya Genetik Lokal di Kabupaten Jeneponto. Prosiding seminar nasional sumber daya genetik pertanian 363-369.
    [BibTeX] [Abstract] [PDF: Keragaan dan Pemanfaatan Sumber Daya Genetik Lokal di Kabupaten Jeneponto ]
    Genetic resources is a collection of diversity (phenotypic and genotypic) in each plant species. The diversity differs between locations and have unique characteristic in accordance with local agroecology, such as the management and utilization of different. The aim of this study is to determine the variabilities of local genetic resources and their utilization by local communities in Jeneponto district. The study was conducted from February to May 2013. Documenting of existing germplasm diversity in their yards and outside grounds (gardens, fields) conducted by direct observation in the field and interviews with respondents using questionnaires. Selected samples consist of 30 farmer households. Data were tabulated and presented descriptively. Inventory results of genetic resources in Jeneponto, a number of 36 species of plants was obtained. Crop group consists of four species, namely rice, maize, cassava and taro. Horticultural crops group consisted of 23 species and 6 species of tree crops. Dominant commodities cultivated by farmers, namely moringa, mango, sugar apple and passionfruit lowlands, black rice (Pare Punu Le’Leng), local maize (God Koasa), Peanut pigeonpea (bintotoeng), as well as lontara tree (palm). These commodities have a high economic value to be developed to support of local food security.
    @article{andSahardi15p363,
    title = {{Keragaan dan Pemanfaatan Sumber Daya Genetik Lokal di Kabupaten Jeneponto}},
    author = {Maintang, Fadjry Djufry, and Sahardi},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {363-369},
    year = {2015},
    abstract = {Genetic resources is a collection of diversity (phenotypic and genotypic) in each plant species. The diversity differs between locations and have unique characteristic in accordance with local agroecology, such as the management and utilization of different. The aim of this study is to determine the variabilities of local genetic resources and their utilization by local communities in Jeneponto district. The study was conducted from February to May 2013. Documenting of existing germplasm diversity in their yards and outside grounds (gardens, fields) conducted by direct observation in the field and interviews with respondents using questionnaires. Selected samples consist of 30 farmer households. Data were tabulated and presented descriptively. Inventory results of genetic resources in Jeneponto, a number of 36 species of plants was obtained. Crop group consists of four species, namely rice, maize, cassava and taro. Horticultural crops group consisted of 23 species and 6 species of tree crops. Dominant commodities cultivated by farmers, namely moringa, mango, sugar apple and passionfruit lowlands, black rice (Pare Punu Le'Leng), local maize (God Koasa), Peanut pigeonpea (bintotoeng), as well as lontara tree (palm). These commodities have a high economic value to be developed to support of local food security.},
    keywords = {local genetic resources, inventory, economic value, utilization},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/41-Maintang - BPTP Sulawese Selatan.pdf}
    }
  42. Asaad, Muh. and Patta Sija. 2015. Inventarisasi Sumber Daya Genetik tanaman Lokal Kabupaten Bone Bolango Provinsi Gorontalo. Prosiding seminar nasional sumber daya genetik pertanian 370-376.
    [BibTeX] [Abstract] [PDF: Inventarisasi Sumber Daya Genetik tanaman Lokal Kabupaten Bone Bolango Provinsi Gorontalo ]
    Agricultural genetic resources management program which includes its preservation and utilization need information of the status and distribution of genetic resources. Inventory of local plant genetic resources in the farmer’s yards, fields and gardens collection needs to be conducted in Gorontalo to obtain information of level of diversity. The study was conducted in June 2013 in three sub district in the Bone Bolango district, i.e. sub-district of Bulango Ulu, East Suwawa and South Suwawa. The survey was conducted to 30 farmers who are selected by using stratification method based on the distance of their county seat and type of road. Data was analyzed to find the Shannon index (H), the Equitability Index (EH) and the Sorensen coefficient (SC). The inventory activity result in the total of 70 PGR including food crops, horticulture, estate crops, ornamental, spices and medicine. Data analysis showed that diversity of PGR was relatively low, indicated by Shannon index value (H) which was ranged from 0.6219 to 0.8972. The PGR was also not evenly distributed in the districts with a level of EH ranged from 0.1831 to 0.2400. The structure of PGR among the three districts shows no- similarity, indicated by the SC value which was ranged from 0.3582 to 0.4516.
    @article{Asaad15p370,
    title = {{Inventarisasi Sumber Daya Genetik tanaman Lokal Kabupaten Bone Bolango Provinsi Gorontalo}},
    author = {Muh. Asaad and Patta Sija},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {370-376},
    year = {2015},
    abstract = {Agricultural genetic resources management program which includes its preservation and utilization need information of the status and distribution of genetic resources. Inventory of local plant genetic resources in the farmer's yards, fields and gardens collection needs to be conducted in Gorontalo to obtain information of level of diversity. The study was conducted in June 2013 in three sub district in the Bone Bolango district, i.e. sub-district of Bulango Ulu, East Suwawa and South Suwawa. The survey was conducted to 30 farmers who are selected by using stratification method based on the distance of their county seat and type of road. Data was analyzed to find the Shannon index (H), the Equitability Index (EH) and the Sorensen coefficient (SC). The inventory activity result in the total of 70 PGR including food crops, horticulture, estate crops, ornamental, spices and medicine. Data analysis showed that diversity of PGR was relatively low, indicated by Shannon index value (H) which was ranged from 0.6219 to 0.8972. The PGR was also not evenly distributed in the districts with a level of EH ranged from 0.1831 to 0.2400. The structure of PGR among the three districts shows no- similarity, indicated by the SC value which was ranged from 0.3582 to 0.4516.},
    keywords = {plant genetic resources, bone bolango district, diversity},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/42-Muh Asaad - BPTP Gorontalo.pdf}
    }
  43. Abdul Wahid Rauf Herman Tata and Muhammad Arif Arbianto. 2015. Keragaman Sumber Daya genetik Tanaman Spesifik Lokal Kabupaten Manokwari Papua Barat. Prosiding seminar nasional sumber daya genetik pertanian 377-387.
    [BibTeX] [Abstract] [PDF: Keragaman Sumber Daya genetik Tanaman Spesifik Lokal Kabupaten Manokwari Papua Barat ]
    Inventarization of local specific genetic resource implemented to obtain information local specific of genetic variability. Such information can be used as basis for policy development and utilization of genetic resources to improve welfare community. The Inventory was held in Manokwari from March to December 2013, using exploration methods. An exploration activity was conducted both in their farmers’ fields yards and garden. Farmer’s samples were determined using stratified sampling based on agro-ecological zones with the number of 30 farmers as a sample. Data collection was conducted through direct interviews with farmers and the identification of specific plant characters chosen among other food crops, vegetable crops, spices/medicinal crops, and fruit crops. The results showed that the highest diversity of plant genetic resources was obtained on the local sweetpotato with nine accessions. Similarly, the highest population obtained on sweet potato was 15850 populations. Utilization of plant genetic resources, especially food crops is generally used as a staple food sources either by baked or boiled. A genetic resource of medicinal plants such as buah merah (red fruit), kayu akuay (akuay wood), rumput kebar (kebar grass), and daun gatal (Itchi leaf) were generally grown naturally and had not been intensively cultivated by farmers.
    @article{andMuhammadArifArbianto15p377,
    title = {{Keragaman Sumber Daya genetik Tanaman Spesifik Lokal Kabupaten Manokwari Papua Barat}},
    author = {Abdul Wahid Rauf, Herman Tata, and Muhammad Arif Arbianto},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {377-387},
    year = {2015},
    abstract = {Inventarization of local specific genetic resource implemented to obtain information local specific of genetic variability. Such information can be used as basis for policy development and utilization of genetic resources to improve welfare community. The Inventory was held in Manokwari from March to December 2013, using exploration methods. An exploration activity was conducted both in their farmers' fields yards and garden. Farmer's samples were determined using stratified sampling based on agro-ecological zones with the number of 30 farmers as a sample. Data collection was conducted through direct interviews with farmers and the identification of specific plant characters chosen among other food crops, vegetable crops, spices/medicinal crops, and fruit crops. The results showed that the highest diversity of plant genetic resources was obtained on the local sweetpotato with nine accessions. Similarly, the highest population obtained on sweet potato was 15850 populations. Utilization of plant genetic resources, especially food crops is generally used as a staple food sources either by baked or boiled. A genetic resource of medicinal plants such as buah merah (red fruit), kayu akuay (akuay wood), rumput kebar (kebar grass), and daun gatal (Itchi leaf) were generally grown naturally and had not been intensively cultivated by farmers.},
    keywords = {inventarization, genetic resources, local specific, west papua},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/43-Abdul Wahid Rauf - BPTP Papua Barat.pdf}
    }
  44. Astarina, Reni and Sri Swastika. 2015. Hubungan Antara Etnik dengan Keragaman Jenis SDG di Lahan Pekarangan di Kabupaten Rokan Hulu Provinsi Riau. Prosiding seminar nasional sumber daya genetik pertanian 388-392.
    [BibTeX] [Abstract] [PDF: Hubungan Antara Etnik dengan Keragaman Jenis SDG di Lahan Pekarangan di Kabupaten Rokan Hulu Provinsi Riau ]
    The objective of this study were to: (1) obtain the information about the collection of each household in the yard, (2) know the correlation of the various ethnic and the plant diversity in the yard,the study was carried out in Rokan Hulu in 4 (four) sub districts : Rambah Samo, Rambah, Ujung batu and Rambah Hilir. This study method survey with stratified random sampling techniques. The sample consist of 30 people which is determined randomly. The information for the primary data consist of the personal information of the farmer, name of plant, number of each type of plant, extensive of the yard and the various ethnic. Data were analyzed using tabulation with correlation coefficient. The results showed that: (1) the collection of each household dominated by food crops, rice and corn, horticulture : snake fruit, sawo, matoa, banana, rambutan, adenium, herbs, sansiviera, anthurium, plantation crops dominated by rubber, (2) The correlation is positive for the various ethnic and the plant diversity in the yard. In the aggregate the Javanese tend to plant the horticulture crops, Malay and Minang plant the herbs, Sundanese plant the food crops and Batak plant the plantation crops, (3). The Javanese plants variety were the highest followed by Malay and Minang, Sundanese and Batak.
    @article{ReniAstarina15p388,
    title = {{Hubungan Antara Etnik dengan Keragaman Jenis SDG di Lahan Pekarangan di Kabupaten Rokan Hulu Provinsi Riau}},
    author = {Reni Astarina and Sri Swastika},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {388-392},
    year = {2015},
    abstract = {The objective of this study were to: (1) obtain the information about the collection
    of each household in the yard, (2) know the correlation of the various ethnic and
    the plant diversity in the yard,the study was carried out in Rokan Hulu in 4 (four)
    sub districts : Rambah Samo, Rambah, Ujung batu and Rambah Hilir. This study
    method survey with stratified random sampling techniques. The sample consist of
    30 people which is determined randomly. The information for the primary data
    consist of the personal information of the farmer, name of plant, number of each
    type of plant, extensive of the yard and the various ethnic. Data were analyzed
    using tabulation with correlation coefficient. The results showed that: (1) the
    collection of each household dominated by food crops, rice and corn, horticulture :
    snake fruit, sawo, matoa, banana, rambutan, adenium, herbs, sansiviera, anthurium,
    plantation crops dominated by rubber, (2) The correlation is positive for the various
    ethnic and the plant diversity in the yard. In the aggregate the Javanese tend to
    plant the horticulture crops, Malay and Minang plant the herbs, Sundanese plant
    the food crops and Batak plant the plantation crops, (3). The Javanese plants
    variety were the highest followed by Malay and Minang, Sundanese and Batak.},
    keywords = {various ethnics, plant diversity, yard},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/44-Reni Astarina - BPTP Riau.pdf}
    }
  45. I Made Rai Yasa A.A.N.B. Kamandalu and I. N. Adijaya. 2015. Potensi Permasalahan Pengembangan Kambing Gembrong di Provinsi Bali Berdasarkan pendekatan Participatory Rural Appraisal (PRA). Prosiding seminar nasional sumber daya genetik pertanian 393-401.
    [BibTeX] [Abstract] [PDF: Potensi Permasalahan Pengembangan Kambing Gembrong di Provinsi Bali Berdasarkan pendekatan Participatory Rural Appraisal (PRA) ]
    Gembrong goat is one of genetic resources of livestock in Bali Province. Currently its population is critical. This activity aims to analyze the potential and problems of development of Gembrong goats. The experiment was conducted in March 2013 in Tumbi village, Sub-district of Karangasem, District of Karangasem, Bali, by using Participatory Rural Appraisal (PRA) involving 35 participants consisting of farmers and related agencies in the scope of District of Karangasem. PRA technique was used in the history of the program, the ranking of the problem, season calendar, and financial analysis. Based on the programmed history, it is known that the conservation efforts of Gembrong goat was began in 1980. Some of these programs were success, but some others were failed and backfire against conservation efforts. Analysis of intuitional techniques recommended that the farmer group of Vishnu Segara need to be empowered, for not merely struggling with conservation efforts but also for business. The membership of the group was reduced from 40 members in 2010 into 20 members in 2013. This group was also not having basic administrative rule, so that this groups have not run properly. Support from several agencies is also still partial in their own ways and not integrated, such that the conservation programmed are not sustainable. In the future, integrated and sustainable programmers needed. Techniques of season calendar showed no differences in the prominent feed, breeding season, season of birth, and disease among the dry rainy season. Financial analysis showed that this business of goat rearing has not been profitable (R/C ratio of 0.9). Assistance is necessary to seek alternative of income to sustain the interest of farmers to preserve Gembrong goat.
    @article{Adijaya15p393,
    title = {{Potensi Permasalahan Pengembangan Kambing Gembrong di Provinsi Bali Berdasarkan pendekatan Participatory Rural Appraisal (PRA)}},
    author = {I Made Rai Yasa, A.A.N.B. Kamandalu, and I N. Adijaya},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {393-401},
    year = {2015},
    abstract = {Gembrong goat is one of genetic resources of livestock in Bali Province. Currently its population is critical. This activity aims to analyze the potential and problems of development of Gembrong goats. The experiment was conducted in March 2013 in Tumbi village, Sub-district of Karangasem, District of Karangasem, Bali, by using Participatory Rural Appraisal (PRA) involving 35 participants consisting of farmers and related agencies in the scope of District of Karangasem. PRA technique was used in the history of the program, the ranking of the problem, season calendar, and financial analysis. Based on the programmed history, it is known that the conservation efforts of Gembrong goat was began in 1980. Some of these programs were success, but some others were failed and backfire against conservation efforts. Analysis of intuitional techniques recommended that the farmer group of Vishnu Segara need to be empowered, for not merely struggling
    with conservation efforts but also for business. The membership of the group was
    reduced from 40 members in 2010 into 20 members in 2013. This group was also
    not having basic administrative rule, so that this groups have not run properly.
    Support from several agencies is also still partial in their own ways and not
    integrated, such that the conservation programmed are not sustainable. In the
    future, integrated and sustainable programmers needed. Techniques of season
    calendar showed no differences in the prominent feed, breeding season, season of
    birth, and disease among the dry rainy season. Financial analysis showed that this
    business of goat rearing has not been profitable (R/C ratio of 0.9). Assistance is
    necessary to seek alternative of income to sustain the interest of farmers to preserve
    Gembrong goat.},
    keywords = {Gembrong goat, Bali, Participatory Rural AppraisalGembrong goat, Bali, Participatory Rural Appraisal},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/45-I Made Rai Yasa - BPTP Bali.pdf}
    }
  46. Guntoro, Suprio and Made I. Londra. 2015. Keragaan Reproduksi dan Produksi Kambing Genbrong. Prosiding seminar nasional sumber daya genetik pertanian 402-407.
    [BibTeX] [Abstract] [PDF: Keragaan Reproduksi dan Produksi Kambing Genbrong ]
    Gembrong is a local goat of Bali which has specific characteristics, now is on the verge of extinction. The appropriate research on it had been conducted to determine the performance of reproduction and production of Gembrong in 2008- 2010 at the Sawe village, in the district of Jembrana. Research was continued in 2010-2012 in Tumbu village, in the district of Karangasem with the same sample sets. The research material consisted of six female and six male Gembrong goats. Goats were kept in stilts cages which consisted of bulkheads, each goat was placed in the bulkhead. Feed consisted of grass forage mixture, Gamal, legume, ad libitum and rice bran concentrate 150 g/goat/day. The observed parameters were estrus cycle, the old lust, pregnant period, litter size, birth weight, sex ratio of child, child mortality, child growth, and weight of adult goats. Observed data were analyzed descriptively. The result of the research showed that female goat estrus cycle was 19-22 days, with the old lust 30-36 hours. Pregnant period was 146 days (142-151 days), litter size was 1.25 with 1.33 kg birth weight and sex ratio of 56% (males) and 44% (females). Child mortality was very high at 62.50% with the highest mortality rates was taken place in lactating (41.67%), give birth (12.50%), and post-weaning phase (8.33%). Weight accretiongained until the age of 4 months (112 days) was 48 g/goat/day in male and 43 g/goat/day in female. In average, weight of adult goats was 31.20 kg in male and 20.67 kg in female.
    @article{SuprioGuntoro15p402,
    title = {{Keragaan Reproduksi dan Produksi Kambing Genbrong}},
    author = {Suprio Guntoro and I Made Londra},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {402-407},
    year = {2015},
    abstract = {Gembrong is a local goat of Bali which has specific characteristics, now is on the verge of extinction. The appropriate research on it had been conducted to determine the performance of reproduction and production of Gembrong in 2008- 2010 at the Sawe village, in the district of Jembrana. Research was continued in 2010-2012 in Tumbu village, in the district of Karangasem with the same sample sets. The research material consisted of six female and six male Gembrong goats. Goats were kept in stilts cages which consisted of bulkheads, each goat was placed in the bulkhead. Feed consisted of grass forage mixture, Gamal, legume, ad libitum and rice bran concentrate 150 g/goat/day. The observed parameters were estrus cycle, the old lust, pregnant period, litter size, birth weight, sex ratio of child, child mortality, child growth, and weight of adult goats. Observed data were analyzed descriptively. The result of the research showed that female goat estrus cycle was 19-22 days, with the old lust 30-36 hours. Pregnant period was 146 days (142-151 days), litter size was 1.25 with 1.33 kg birth weight and sex ratio of 56% (males) and 44% (females). Child mortality was very high at 62.50% with the highest mortality rates was taken place in lactating (41.67%), give birth (12.50%), and post-weaning phase (8.33%). Weight accretiongained until the age of 4 months (112 days) was 48 g/goat/day in male and 43 g/goat/day in female. In average, weight of adult goats was 31.20 kg in male and 20.67 kg in female.},
    keywords = {gembrong, goat, reproduction, production},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/46-Suprio Guntoro-I Made Londra - BPTP Bali.pdf}
    }
  47. Suyasa, Nyoman and Ida Ayu Parwati. 2015. Karakterisasi Kambing Genbrong Bali. Prosiding seminar nasional sumber daya genetik pertanian 408-414.
    [BibTeX] [Abstract] [PDF: Karakterisasi Kambing Genbrong Bali ]
    Goat is one of the mainstays in the provision of livestock animal protein in Indonesia. Gembrong goat is one of the potential genetic resources that began to received attention because of the uniqueness and its populations is in critical. This research was conducted in Sawe village, in the district of Jembrana by using 9 males and 8 females. In this experiment, feed were given as usual forage plus concentrate only in the form of rice bran 200 g/goat/day as a supplement feed and de-worming at the time of study entry. Weight and birth weight were weighed using scales and fur length was measured using a meter. The results of research showed that the average male weight was 29.15 kg while the female was 18.20 kg. Weaning weight of male children was 1.23 and females 1.30 kg, horn length male and female were 13.17 and 4.40 cm, respectively The dominance of white fur reached 94.12%, with the male hair length from 9.14 to 11.07 and 19.79 cm beard, while in females from 5.30 to 8.80 and 7.30 cm beard. From the analysis of blood components between Gembrong, Kacang and PE goats, it showed that there was not much different except for triglyceride content.
    @article{NyomanSuyasa15p408,
    title = {{Karakterisasi Kambing Genbrong Bali}},
    author = {Nyoman Suyasa and Ida Ayu Parwati},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {408-414},
    year = {2015},
    abstract = {Goat is one of the mainstays in the provision of livestock animal protein in Indonesia. Gembrong goat is one of the potential genetic resources that began to received attention because of the uniqueness and its populations is in critical. This research was conducted in Sawe village, in the district of Jembrana by using 9 males and 8 females. In this experiment, feed were given as usual forage plus concentrate only in the form of rice bran 200 g/goat/day as a supplement feed and de-worming at the time of study entry. Weight and birth weight were weighed using scales and fur length was measured using a meter. The results of research showed that the average male weight was 29.15 kg while the female was 18.20 kg. Weaning weight of male children was 1.23 and females 1.30 kg, horn length male and female were 13.17 and 4.40 cm, respectively The dominance of white fur reached 94.12%, with the male hair length from 9.14 to 11.07 and 19.79 cm beard, while in females from 5.30 to 8.80 and 7.30 cm beard. From the analysis of blood components between Gembrong, Kacang and PE goats, it showed that there was not much different except for triglyceride content.},
    keywords = {gembrong goat, critical population, body weight, birth weight},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/47-Nyoman Suyasa - BPTP Bali.pdf}
    }
  48. Sugama, Nyoman I. and I. N. Suyasa. 2015. Karakteristik Morfologis Ayam Buras Bali. Prosiding seminar nasional sumber daya genetik pertanian 415-424.
    [BibTeX] [Abstract] [PDF: Karakteristik Morfologis Ayam Buras Bali ]
    Bali chicken is a local race that has important prospect for social, economic and cultural. However, it generally grows less intensively, so that productivity is low. Therefore, it needs effort to preserve and develop as it is part of Indonesian germplasm. This study was conducted to determine the morphological characteristics of domestic poultry. The study was conducted at the site of Sawe Rangsasa breeding center, Dauh Waru village, subdistrict of Jembrana, Jembrana district. The samples were 50 chickens from F3 derivate. Feed provided included : starter phase in the form of concentrate feed BR.1 10-20 g/head/day (up to 1 month of age chicken); grower phase in the form of concentrate feed mixture BR.1 + grilled corn bran with a composition of 25% : 45% : 30%, with the amount of 40- 50 g/head/day (chicken age 1-4 months); and layer/finisher phase in the form of concentrate feed mixture K.124 + grilled corn bran with a composition of 37% : 45% : 18%, as much as 70-80 g/head/day (chicken age 4-6 months). Parameters measured were the productivity and morphological characteristics. The results showed that the fastest growth of domestic poultry Bali F3 occurred from the first month, with an average weight gained per cow/day of 7,131 grams. Weight of 1kg piece achieved at the age of 20 weeks. Selected egg productivity (F3) is quite high (46.75%), with 78.40% fertility and hatchability of eggs 78%. The chemical composition of eggs was protein (11.26%), fat (16.05%), water content (1.08%), water content (69.20%) and carbohydrates (2.72%). The percentage of carcass weight to live weight of 65 185%. The percentage of carcass weight constituent organs such as chest, back, thighs, calves, and the wings of the carcass weight were 26.10%, 25.45%, 16.96%, 16.23%, and 13.71%, respectively.
    @article{INyomanSugama15p415,
    title = {{Karakteristik Morfologis Ayam Buras Bali}},
    author = {I Nyoman Sugama and I N. Suyasa},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {415-424},
    year = {2015},
    abstract = {Bali chicken is a local race that has important prospect for social, economic and cultural. However, it generally grows less intensively, so that productivity is low. Therefore, it needs effort to preserve and develop as it is part of Indonesian germplasm. This study was conducted to determine the morphological characteristics of domestic poultry. The study was conducted at the site of Sawe Rangsasa breeding center, Dauh Waru village, subdistrict of Jembrana, Jembrana district. The samples were 50 chickens from F3 derivate. Feed provided included : starter phase in the form of concentrate feed BR.1 10-20 g/head/day (up to 1 month of age chicken); grower phase in the form of concentrate feed mixture BR.1 + grilled corn bran with a composition of 25% : 45% : 30%, with the amount of 40- 50 g/head/day (chicken age 1-4 months); and layer/finisher phase in the form of concentrate feed mixture K.124 + grilled corn bran with a composition of 37% : 45% : 18%, as much as 70-80 g/head/day (chicken age 4-6 months). Parameters measured were the productivity and morphological characteristics. The results showed that the fastest growth of domestic poultry Bali F3 occurred from the first month, with an average weight gained per cow/day of 7,131 grams. Weight of 1kg
    piece achieved at the age of 20 weeks. Selected egg productivity (F3) is quite high
    (46.75%), with 78.40% fertility and hatchability of eggs 78%. The chemical
    composition of eggs was protein (11.26%), fat (16.05%), water content (1.08%),
    water content (69.20%) and carbohydrates (2.72%). The percentage of carcass
    weight to live weight of 65 185%. The percentage of carcass weight constituent
    organs such as chest, back, thighs, calves, and the wings of the carcass weight were
    26.10%, 25.45%, 16.96%, 16.23%, and 13.71%, respectively.},
    keywords = {Bali domestic poultry, productivity characteristics, morphological
    characteristics},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/48-I Nyoman Sugama - BPTP Bali.pdf}
    }
  49. I Made Rai Yasa I Nyoman Adijaya and Putu Agus K. Wirawan. 2015. Pptensi dan Permasalahan Pengembangan Sapi Taro(Sapi Bali Putih) di Desa Taro Kabupaten Gianyar Bali. Prosiding seminar nasional sumber daya genetik pertanian 425-431.
    [BibTeX] [Abstract] [PDF: Pptensi dan Permasalahan Pengembangan Sapi Taro(Sapi Bali Putih) di Desa Taro Kabupaten Gianyar Bali ]
    Taro cattle is a synonim name of White Bali cattle in the region of Taro, Taro village, Tegalalang subdistrict, in the district of Gianyar, Bali province. Research was conducted at the beginning of June 2014 to determine the potential and future development of Taro cattle. Data were analyzed by descriptively and through dynamic systems by using Powersim Cconstructor 2,5d. The results showed that Taro cattle has some uniqueness among others, such as having birth on certain days (Purnama, Tilem, or Kajeng Kliwon); it has given names in Balinese high-social class Brahmana, such as Ida Bagus (for male) and Ida Ayu (for female); there will be religion ceremonies for the birth, six months age, and when it was buried. In addition, this cattle is reared by ngayah (traditional mutual aid) in rotation by the local community. This catttle is also forbidden to be employed, sale, consumption for its meat and milk, and if violated there would be a disastes. These special treatments actually have the potential support for the success of conservation of Taro cattle. Although Taro cattle population at this time only 34 individuals (16 males, 12 females and 6 child) with critical status of population, the population could reach 306, including 104 females in 2025. The increasing of population would require more feed, labor for ngayah, cages, and lands. Therefore, it is necessary to formulate a good plan for its future development.
    @article{Wirawan15p425,
    title = {{Pptensi dan Permasalahan Pengembangan Sapi Taro(Sapi Bali Putih) di Desa Taro Kabupaten Gianyar Bali}},
    author = {I Made Rai Yasa, I Nyoman Adijaya, and Putu Agus K. Wirawan},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {425-431},
    year = {2015},
    abstract = {Taro cattle is a synonim name of White Bali cattle in the region of Taro, Taro village, Tegalalang subdistrict, in the district of Gianyar, Bali province. Research was conducted at the beginning of June 2014 to determine the potential and future development of Taro cattle. Data were analyzed by descriptively and through dynamic systems by using Powersim Cconstructor 2,5d. The results showed that Taro cattle has some uniqueness among others, such as having birth on certain days (Purnama, Tilem, or Kajeng Kliwon); it has given names in Balinese high-social class Brahmana, such as Ida Bagus (for male) and Ida Ayu (for female); there will be religion ceremonies for the birth, six months age, and when it was buried. In addition, this cattle is reared by ngayah (traditional mutual aid) in rotation by the local community. This catttle is also forbidden to be employed, sale, consumption for its meat and milk, and if violated there would be a disastes. These special treatments actually have the potential support for the success of conservation of Taro cattle. Although Taro cattle population at this time only 34 individuals (16 males, 12 females and 6 child) with critical status of population, the population could reach 306, including 104 females in 2025. The increasing of population would require more feed, labor for ngayah, cages, and lands. Therefore, it is necessary to formulate a good plan for its future development.},
    keywords = {taro cattle, bali, preservation},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/49-I Made Rai Yasa - BPTP Bali.pdf}
    }
  50. Arsana, Gusti Komang Dana I.. 2015. Perkembangan Kegiatan Konsorsium Penguatan program Pengelolaan Sumber Daya Genetik di Bali (Pengelolaan Sumber Daya Genetik di Bali). Prosiding seminar nasional sumber daya genetik pertanian 432-439.
    [BibTeX] [Abstract] [PDF: Perkembangan Kegiatan Konsorsium Penguatan program Pengelolaan Sumber Daya Genetik di Bali (Pengelolaan Sumber Daya Genetik di Bali) ]
    Bali Provincial rich in biodiversity with abundance of plant genetic resources and some of which is an endemic plant. Some of these genetic resources have now been successfully developed and commercially distributed. However some others are underutilized and endangered. Efforts through counseling, coaching and building of conservation centre are needed to conserve these genetic resources and to foster the utilization and plant development. This study was subjected to observe and to record the availability of plant genetic resources in Bali Province. Data were collected based on the information obtained from farmers that were surveyed in eight sub-districts in Bali province. Survey showed the distribution of various genetic resources i.e. food crop (12), plant-herb spices (8), fodder plant crop (4), estate crop (20), ornamental plant (30), and ritual-ceremonial related plants (19).
    @article{IGustiKomangDanaArsana15p432,
    title = {{Perkembangan Kegiatan Konsorsium Penguatan program Pengelolaan Sumber Daya Genetik di Bali (Pengelolaan Sumber Daya Genetik di Bali)}},
    author = {I Gusti Komang Dana Arsana},
    journal = {Prosiding Seminar Nasional Sumber Daya Genetik Pertanian},
    pages = {432-439},
    year = {2015},
    abstract = {Bali Provincial rich in biodiversity with abundance of plant genetic resources and some of which is an endemic plant. Some of these genetic resources have now been successfully developed and commercially distributed. However some others are underutilized and endangered. Efforts through counseling, coaching and building of conservation centre are needed to conserve these genetic resources and to foster the utilization and plant development. This study was subjected to observe and to record the availability of plant genetic resources in Bali Province. Data were collected based on the information obtained from farmers that were surveyed in eight sub-districts in Bali province. Survey showed the distribution of various genetic resources i.e. food crop (12), plant-herb spices (8), fodder plant crop (4), estate crop (20), ornamental plant (30), and ritual-ceremonial related plants (19).},
    keywords = {genetic resources, food crops, estate crops, medicinal plants},
    file = {D:/Document/Tim situs Biogen/2015-11/Prosiding Seminar Nasional SDG.PDF/50-I Gusti Komang Dana Arsana - BPTP Bali.pdf}
    }

2006

  1. Makarim, A. K.. 2006. Cekaman Abiotik Utama dalam Peningkatan Produktivitas Tanaman. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 1-11.
    [BibTeX] [Abstract] [PDF: Cekaman Abiotik Utama dalam Peningkatan Produktivitas Tanaman ]
    The needs for agricultural products are increasing in quantity and quality with increasing the demand of market and of population. Agricultural expansion and/or crop productivity improvement may face various limiting factors, mainly abiotic stresses that may limit crop production or even crop production failure. The main abiotic stress factor is drought which is predicted more severely occurred in the future due to water is highly used by the other users of non-agricultural sectors, decreasing soil water holding capacity, and environmental deterioration. Opening new agricultural lands are mostly acid dry land and tidal swampy areas which having various abiotic problems such as Al, Mn, Fe, sulphate, Na, Cl toxicities, and/or N, P, K, Ca, Mg, Mo deficiencies, low pH, high pH those should be overcome. The other abiotic stresses appear and widespread such as salinity, sea water intrusion, Fe toxicity, highly reductive soils, etc. as the results of mismanagement, soil degradation or environmental process results. Therefore, crop variety improvement through breeding or biotechnology to overcome the above abiotic stresses are urgently needed. Those methods are proved effective, efficient and adoptable by farmers. Integrated soil-crop management may be used to overcome those problems at certain level only.
    @article{Makarim06p1,
    title = {{Cekaman Abiotik Utama dalam Peningkatan Produktivitas Tanaman}},
    author = {A. K. Makarim},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {1-11},
    year = {2006},
    abstract = {The needs for agricultural products are increasing in quantity and quality with increasing the demand of market and of population. Agricultural expansion and/or crop productivity improvement may face various limiting factors, mainly abiotic stresses that may limit crop production or even crop production failure. The main abiotic stress factor is drought which is predicted more severely occurred in the future due to water is highly used by the other users of non-agricultural sectors, decreasing soil water holding capacity, and environmental deterioration. Opening new agricultural lands are mostly acid dry land and tidal swampy areas which having various abiotic problems such as Al, Mn, Fe, sulphate, Na, Cl toxicities, and/or N, P, K, Ca, Mg, Mo deficiencies, low pH, high pH those should be overcome. The other abiotic stresses appear and widespread such as salinity, sea water intrusion, Fe toxicity, highly reductive soils, etc. as the results of mismanagement, soil degradation or environmental process results. Therefore, crop variety improvement through breeding or biotechnology to overcome the above abiotic stresses are urgently needed. Those methods are proved effective, efficient and adoptable by farmers. Integrated soil-crop management may be used to overcome those problems at certain level only.},
    keywords = {abiotic stress, drought, al toxicity},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_1-11.pdf}
    }
  2. Suharsono. 2006. Eksplorasi Gen-gen Toleran Cekaman Abiotik pada Tanaman. Seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 13-27.
    [BibTeX] [Abstract] [PDF: Eksplorasi Gen-gen Toleran Cekaman Abiotik pada Tanaman ]
    Produksi pertanian dapat ditingkatkan melalui program intensifikasi dan ekstensifikasi. Baik program intensifikasi maupun program ekstensifikasi membutuhkan kultivar unggul. Kultivar unggul dapat dirakit melalui perbaikan genetik baik melalui persilangan konven-sional maupun melalui teknologi DNA rekombinan. Program ekstensifikasi pertanian terbentur pada terbatasnya lahan yang sesuai. Beralih-fungsinya lahan pertanian menjadi pemukiman atau kawasan industri, terutama di pulau Jawa, merupakan salah satu faktor yang menyebabkan lahan pertanian menjadi sem-pit. Oleh sebab itu, pembukaan lahan baru untuk pertanian harus dilakukan. Namun, pem-bukaan lahan baru yang belum dimanfaatkan dengan optimal, terutama di luar Pulau Jawa, mempunyai banyak kendala, karena sebagian besar lahan adalah lahan marginal seperti lahan asam, lahan garam, lahan yang sangat kering. Perbaikan genetik sangat tergantung dari sumber bahan genetik, khususnya gen. Analisis molekuler terhadap tanaman yang toleran terhadap suatu cekaman, sangat mendukung program perbaikan genetik tanaman yang dapat beradaptasi pada kondisi lahan marginal tertentu. Isolasi gen-gen yang berhubungan dengan toleransi tanaman terhadap suatu cekam-an sangat penting untuk perbaikan genetik tanaman dalam rangka peningkatan produksi pertanian. Toleransi terhadap suatu cekaman abiotik adalah suatu sifat yang kompleks yang dikendalikan oleh beberapa yang berbeda yang terkoordinasi melalui suatu sistem jejaring. Gen-gen yang berhubungan dengan sistem toleransi tanaman terhadap suatu cekaman dapat diisolasi dari tumbuhan yang tumbuh di tanah marginal dengan menggunakan gen dari spesies lain yang ekspresinya diinduksi oleh cekaman tersebut sebagai pelacak. Melalui penapisan diferensial terhadap tanaman near iso line (NIL) yang mendapat cekaman, gen yang berhubungan dengan cekaman dapat dideterminasi dan diisolasi. Penapisan diferensial terhadap pustaka cDNA dari tanaman yang mendapat cekaman dan yang tidak mendapat cekaman sangat banyak digunakan dalam usaha isolasi gen. Dengan menggunakan pelacak dari spesies lain, penapisan terhadap pustaka genom juga sering di-lakukan untuk mendapatkan gen yang utuh. Dalam rangka menyimpan seluruh bahan gene-tik kedelai lokal Indonesia, pustaka genom kedelai kultivar Slamet dan kultivar Lumut telah dikonstruksi (Suharsono 2002; Suharsono dan Jusuf 2003). Active Oxygen Species (AOS) seperti radikal superoksida (O2-), H2O2, dan radikal hidroksil (OH-) dapat mengganggu fungsi DNA, protein, membran, dan klorofil. Cekaman abiotik dapat menyebabkan peningkatan AOS. Untuk mengatasi masalah ini tanaman mengembangkan sistem kompleks antioksidan.
    @article{Suharsono06p13,
    title = {{Eksplorasi Gen-gen Toleran Cekaman Abiotik pada Tanaman}},
    author = {Suharsono},
    journal = {Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {13-27},
    year = {2006},
    abstract = {Produksi pertanian dapat ditingkatkan melalui program intensifikasi dan ekstensifikasi. Baik program intensifikasi maupun program ekstensifikasi membutuhkan kultivar unggul. Kultivar unggul dapat dirakit melalui perbaikan genetik baik melalui persilangan konven-sional maupun melalui teknologi DNA rekombinan.
    Program ekstensifikasi pertanian terbentur pada terbatasnya lahan yang sesuai. Beralih-fungsinya lahan pertanian menjadi pemukiman atau kawasan industri, terutama di pulau Jawa, merupakan salah satu faktor yang menyebabkan lahan pertanian menjadi sem-pit. Oleh sebab itu, pembukaan lahan baru untuk pertanian harus dilakukan. Namun, pem-bukaan lahan baru yang belum dimanfaatkan dengan optimal, terutama di luar Pulau Jawa, mempunyai banyak kendala, karena sebagian besar lahan adalah lahan marginal seperti lahan asam, lahan garam, lahan yang sangat kering. Perbaikan genetik sangat tergantung dari sumber bahan genetik, khususnya gen. Analisis molekuler terhadap tanaman yang toleran terhadap suatu cekaman, sangat mendukung program perbaikan genetik tanaman yang dapat beradaptasi pada kondisi lahan marginal tertentu.
    Isolasi gen-gen yang berhubungan dengan toleransi tanaman terhadap suatu cekam-an sangat penting untuk perbaikan genetik tanaman dalam rangka peningkatan produksi pertanian. Toleransi terhadap suatu cekaman abiotik adalah suatu sifat yang kompleks yang dikendalikan oleh beberapa yang berbeda yang terkoordinasi melalui suatu sistem jejaring. Gen-gen yang berhubungan dengan sistem toleransi tanaman terhadap suatu cekaman dapat diisolasi dari tumbuhan yang tumbuh di tanah marginal dengan menggunakan gen dari spesies lain yang ekspresinya diinduksi oleh cekaman tersebut sebagai pelacak.
    Melalui penapisan diferensial terhadap tanaman near iso line (NIL) yang mendapat cekaman, gen yang berhubungan dengan cekaman dapat dideterminasi dan diisolasi. Penapisan diferensial terhadap pustaka cDNA dari tanaman yang mendapat cekaman dan yang tidak mendapat cekaman sangat banyak digunakan dalam usaha isolasi gen. Dengan menggunakan pelacak dari spesies lain, penapisan terhadap pustaka genom juga sering di-lakukan untuk mendapatkan gen yang utuh. Dalam rangka menyimpan seluruh bahan gene-tik kedelai lokal Indonesia, pustaka genom kedelai kultivar Slamet dan kultivar Lumut telah dikonstruksi (Suharsono 2002; Suharsono dan Jusuf 2003).
    Active Oxygen Species (AOS) seperti radikal superoksida (O2-), H2O2, dan radikal hidroksil (OH-) dapat mengganggu fungsi DNA, protein, membran, dan klorofil. Cekaman abiotik dapat menyebabkan peningkatan AOS. Untuk mengatasi masalah ini tanaman mengembangkan sistem kompleks antioksidan. },
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_13-27.pdf}
    }
  3. Mariska, Ika and Endang Gati Lestari. 2006. Seleksi In Vitro untuk Toleransi terhadap Faktor Abiotik pada Tanaman Padi dan Kedelai. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 28-41.
    [BibTeX] [Abstract] [PDF: Seleksi In Vitro untuk Toleransi terhadap Faktor Abiotik pada Tanaman Padi dan Kedelai ]
    Salah satu upaya untuk memenuhi kebutuhan padi dan kedelai nasional adalah dengan melakukan ekstensifikasi penanaman ke lahan marjinal (masam dan kering) yang tersedia cukup luas di Indonesia. Keragaman genetik varietas yang toleran lahan marjinal masih sangat sempit. Sumber ketahanan terhadap lahan masam dan kering masih terbatas. Untuk mengatasi masalah tersebut maka dapat dilakukan melalui seleksi in vitro. Teknologi tersebut merupakan salah satu metode keragaman somaklonal namun lebih efektif dan efisien karena perubahan diarahkan kepada sifat yang diinginkan. Seleksi in vitro pada tanaman kedelai dilakukan pada kalus embriogenik yang diinduksi dari embrio zigotik muda varietas Slamet, Sindoro, dan Wilis kombinasi dengan radiasi sinar gamma 400 rad. Seleksi dilakukan dengan AlCl3.6H2O (0-500 ppm) dan pH media 4. Media MS dimodifikasikan untuk unsur NH4NO3, CaCl2.H2O, KH2PO4 dan Fe tidak dichelat oleh EDTA. Regenerasi dilakukan pada media seleksi melalui jalur embriogenesis somatik. Benih somatik hasil seleksi diuji dengan tanah masam di rumah kaca, dan selanjutnya untuk generasi ke-2 sampai dengan generasi ke-4 diuji di lahan masam di Gajrug (Banten) dan Jasinga (Kabupaten Bogor). Untuk tanaman padi, kalus embriogenik berasal dari embrio zigotik varietas IR64 kombinasi dengan radiasi sinar gamma (0-700 rad). Seleksi dilakukan dengan PEG (BM6000) = 020%. Regenerasi dilakukan pada media seleksi. Biji generasi kedua yang berasal dari somatik kemudian diuji kembali dengan PEG 20%, daya tembus akarnya dengan campuran parafin : vaselin = 60-40% dengan ketebalan 3 mm. Di samping itu diuji kandungan prolinnya serta produksinya dalam kondisi cekaman kekeringan (60% dari kapasitas lapang). Hasil penelitian pada tanaman kedelai menunjukkan adanya kemampuan penurunan daya regenerasi dengan semakin meningkatnya konsentrasi Al. Benih somatik varietas Slamet umumnya mempunyai struktur yang tidak sempurna. Setelah aklimatisasi padi varietas Sindoro dan Wilis diperoleh 39 nomor. Dari 39 nomor tersebut diperoleh 12 nomor dari varietas Sindoro (Al 1000 ppm + 100 rad) yang mampu berproduksi. Generasi ke-2 dari nomor tersebut kemudian diuji di lahan masam. Pengujian di empat lokasi pada empat generasi menunjukkan adanya potensi yang besar untuk mendapatkan galur-galur harapan kedelai yang toleran Al dan pH rendah (lahan masam). Hasil seleksi in vitro pada tanaman padi diperoleh bahwa tidak semua kalus embriogenik dapat beregenerasi membentuk tunas adventif. Setelah dilakukan pengujian di rumah kaca diperoleh 13 somaklon IR64 yang diduga tahan kekeringan berdasarkan uji PEG dan uji daya tembus akar serta kandungan prolin yang tinggi. Setelah diuji lanjut dengan mengevaluasi produksi bulirnya maka diperoleh 8 somaklon yang toleran kekeringan dan produksi bulirnya tinggi, sedangkan kontrolnya tidak dapat berproduksi pada kondisi diberi cekaman kekeringan. Terdapat korelasi antara 3 karakter yang diuji (PEG, daya tembus akar, prolin) dengan toleransi terhadap kekeringan.
    @article{Administrator06p28,
    title = {{Seleksi In Vitro untuk Toleransi terhadap Faktor Abiotik pada Tanaman Padi dan Kedelai}},
    author = {Ika Mariska and Endang Gati Lestari},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {28-41},
    year = {2006},
    abstract = {Salah satu upaya untuk memenuhi kebutuhan padi dan kedelai nasional adalah dengan melakukan ekstensifikasi penanaman ke lahan marjinal (masam dan kering) yang tersedia cukup luas di Indonesia. Keragaman genetik varietas yang toleran lahan marjinal masih sangat sempit. Sumber ketahanan terhadap lahan masam dan kering masih terbatas. Untuk mengatasi masalah tersebut maka dapat dilakukan melalui seleksi in vitro. Teknologi tersebut merupakan salah satu metode keragaman somaklonal namun lebih efektif dan efisien karena perubahan diarahkan kepada sifat yang diinginkan. Seleksi in vitro pada tanaman kedelai dilakukan pada kalus embriogenik yang diinduksi dari embrio zigotik muda varietas Slamet, Sindoro, dan Wilis kombinasi dengan radiasi sinar gamma 400 rad. Seleksi dilakukan dengan AlCl3.6H2O (0-500 ppm) dan pH media 4. Media MS dimodifikasikan untuk unsur NH4NO3, CaCl2.H2O, KH2PO4 dan Fe tidak dichelat oleh EDTA. Regenerasi dilakukan pada media seleksi melalui jalur embriogenesis somatik. Benih somatik hasil seleksi diuji dengan tanah masam di rumah kaca, dan selanjutnya untuk generasi ke-2 sampai dengan generasi ke-4 diuji di lahan masam di Gajrug (Banten) dan Jasinga (Kabupaten Bogor). Untuk tanaman padi, kalus embriogenik berasal dari embrio zigotik varietas IR64 kombinasi dengan radiasi sinar gamma (0-700 rad). Seleksi dilakukan dengan PEG (BM6000) = 020%. Regenerasi dilakukan pada media seleksi. Biji generasi kedua yang berasal dari somatik kemudian diuji kembali dengan PEG 20%, daya tembus akarnya dengan campuran parafin : vaselin = 60-40% dengan ketebalan 3 mm. Di samping itu diuji kandungan prolinnya serta produksinya dalam kondisi cekaman kekeringan (60% dari kapasitas lapang). Hasil penelitian pada tanaman kedelai menunjukkan adanya kemampuan penurunan daya regenerasi dengan semakin meningkatnya konsentrasi Al. Benih somatik varietas Slamet umumnya mempunyai struktur yang tidak sempurna. Setelah aklimatisasi padi varietas Sindoro dan Wilis diperoleh 39 nomor. Dari 39 nomor tersebut diperoleh 12 nomor dari varietas Sindoro (Al 1000 ppm + 100 rad) yang mampu berproduksi. Generasi ke-2 dari nomor tersebut kemudian diuji di lahan masam. Pengujian di empat lokasi pada empat generasi menunjukkan adanya potensi yang besar untuk mendapatkan galur-galur harapan kedelai yang toleran Al dan pH rendah (lahan masam). Hasil seleksi in vitro pada tanaman padi diperoleh bahwa tidak semua kalus embriogenik dapat beregenerasi membentuk tunas adventif. Setelah dilakukan pengujian di rumah kaca diperoleh 13 somaklon IR64 yang diduga tahan kekeringan berdasarkan uji PEG dan uji daya tembus akar serta kandungan prolin yang tinggi. Setelah diuji lanjut dengan mengevaluasi produksi bulirnya maka diperoleh 8 somaklon yang toleran kekeringan dan produksi bulirnya tinggi, sedangkan kontrolnya tidak dapat berproduksi pada kondisi diberi cekaman kekeringan. Terdapat korelasi antara 3 karakter yang diuji (PEG, daya tembus akar, prolin) dengan toleransi terhadap kekeringan.},
    keywords = {In vitro, abiotik, padi, kedelai},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_28-41.pdf}
    }
  4. Falavigna, A. and G. L. Rotino. 2006. Parthenocarpy, a Strategy for Fruit Development under Adverse Environmental Conditions. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 42-51.
    [BibTeX] [Abstract] [PDF: Parthenocarpy, a Strategy for Fruit Development under Adverse Environmental Conditions ]
    In plants fertilized ovule and ovary synthesize auxine phytohormones sustaining seeds and fruit production (Archbold and Dennis 1985). The development of fruit without pollination and fertilization is called parthenocarpy, therefore parthenocarpy fruits are seedless. Parthenocarpy offers the possibility of improving fruit set when environmental conditions are adverse for pollen production, germination and fertilization; moreover in some crops the absence of seeds can improve fruit quality (e.g. banana, eggplant, persimmon, grape, watermelon), while in other ones (e.g. Actinidia) might also improve productivity. Parthenocarpy can have a genetic basis (genetic or natural parthenocarpy) or it can be artificially induced. Genetic parthenocarpy is called obligatory when the expression of the parthenocarpy trait is not influenced by external factors and facultative if it occurs only under conditions adverse for pollination and fertilization. Artificially induced parthenocarpy can be observed in several plant species by treating flowers with plant growth factors or by pollination with incompatible pollen or X-rays irradiated pollen.
    @article{Falavigna06p42,
    title = {{Parthenocarpy, a Strategy for Fruit Development under Adverse Environmental Conditions}},
    author = {A. Falavigna and G. L. Rotino},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {42-51},
    year = {2006},
    abstract = {In plants fertilized ovule and ovary synthesize auxine phytohormones sustaining seeds and fruit production (Archbold and Dennis 1985). The development of fruit without pollination and fertilization is called parthenocarpy, therefore parthenocarpy fruits are seedless. Parthenocarpy offers the possibility of improving fruit set when environmental conditions are adverse for pollen production, germination and fertilization; moreover in some crops the absence of seeds can improve fruit quality (e.g. banana, eggplant, persimmon, grape, watermelon), while in other ones (e.g. Actinidia) might also improve productivity. Parthenocarpy can have a genetic basis (genetic or natural parthenocarpy) or it can be artificially induced. Genetic parthenocarpy is called obligatory when the expression of the parthenocarpy trait is not influenced by external factors and facultative if it occurs only under conditions adverse for pollination and fertilization. Artificially induced parthenocarpy can be observed in several plant species by treating flowers with plant growth factors or by pollination with incompatible pollen or X-rays irradiated pollen.},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_42-51.pdf}
    }
  5. Saragih, E.. 2006. Pengembangan Produk Rekayasa Genetika Berorientasi Komersial: Prospek dan Tantangan. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 52-60.
    [BibTeX] [Abstract] [PDF: Pengembangan Produk Rekayasa Genetika Berorientasi Komersial: Prospek dan Tantangan ]
    Berjuta-juta petani di beberapa negara Asia saat ini memiliki kesempatan baru untuk lebih menyukseskan pemenuhan kebutuhan pangan dan sandang bagi keluarga, masyarakat, dan negara mereka. Kesempatan baru ini adalah dengan menanam benih produk rekayasa genetika atau sering juga disebut benih tanaman biotek. Dari areal 81 juta hektar tanaman biotek yang ditanam pada tahun 2004, sekitar 4,5 juta hektar ditanam di Asia-Pasifik (James 2004). Negara berpenduduk paling padat yakni China dan India telah memberikan kesempatan bagi petani mengadopsi teknologi ini. Bagi Indonesia, hal ini baru merupakan kesempatan potensial yang semestinya tidak disia-siakan. Pemuliaan tanaman yang makin efisien dan teknik-teknik terbaru dalam bioteknologi telah dan akan memungkinkan menghasilkan varietas tanaman dengan produktivitas lebih tinggi, tahan terhadap hama dan penyakit, atau makin tinggi toleransinya terhadap kondisi cekaman (stress) lingkungan, serta ke depan dikembangkan tanaman dengan kandungan nutrisi yang lebih baik. Menurut sebuah studi (Runge dan Ryan 2004), sebanyak 63 negara telah dan sedang melaksanakan litbang tanaman biotek yang meliputi 57 jenis tanaman. Lebih dari separuh jumlah kegiatan litbang tersebut berada di negara berkembang, sekalipun dengan fokus dan prioritas yang berbeda-beda. Akan tetapi, laporan FAO (2004) baru-baru ini mengemukakan bahwa penelitian tanaman transgenik toleran cekaman abiotik (misalnya kekeringan dan salinitas) sangatlah sedikit. Kekeringan dan salinitas merupakan dua faktor pembatas utama pada lahan-lahan marjinal di negara-negara sedang berkembang. Padahal lahan marjinal dengan cekaman abiotik, yang dapat menurunkan hasil produksi atau membatasi realisasi potensi hasil, sangatlah luas. Untuk Indonesia luasnya mungkin mencapai puluhan juta hektar, terlebih lagi kalau mencakup faktor pembatas kemasaman tanah akibat kandungan aluminium, sulfat masam dan asam-asam organik yang tinggi seperti misalnya di lahan gambut. Suatu hal yang menarik bahwa dalam forum seminar ini secara khusus dibahas tema pemanfaatan bioteknologi untuk mengatasi cekaman abiotik. Menurut hemat saya, subyek ini termasuk strategis dan penting bagi Indonesia. Dalam makalah ini disajikan suatu telaah prospek dan tantangan yang dihadapi baik dengan belajar dari pengalaman komersialisasi tanaman biotek di Indonesia maupun dengan perspektif yang lebih luas. Juga didiskusikan seputar aspek manajemen dan strategi pengembangan produk, dan aspek regulasi dan iklim usaha. Diskusi tentang prospek suatu teknologi atau produk teknologi tentu tidak terlepas dari potensi aplikasinya.
    @article{Saragih06p52,
    title = {{Pengembangan Produk Rekayasa Genetika Berorientasi Komersial: Prospek dan Tantangan}},
    author = {E. Saragih},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {52-60},
    year = {2006},
    abstract = {Berjuta-juta petani di beberapa negara Asia saat ini memiliki kesempatan baru untuk lebih menyukseskan pemenuhan kebutuhan pangan dan sandang bagi keluarga, masyarakat, dan negara mereka. Kesempatan baru ini adalah dengan menanam benih produk rekayasa genetika atau sering juga disebut benih tanaman biotek. Dari areal 81 juta hektar tanaman biotek yang ditanam pada tahun 2004, sekitar 4,5 juta hektar ditanam di Asia-Pasifik (James 2004). Negara berpenduduk paling padat yakni China dan India telah memberikan kesempatan bagi petani mengadopsi teknologi ini. Bagi Indonesia, hal ini baru merupakan kesempatan potensial yang semestinya tidak disia-siakan. Pemuliaan tanaman yang makin efisien dan teknik-teknik terbaru dalam bioteknologi telah dan akan memungkinkan menghasilkan varietas tanaman dengan produktivitas lebih tinggi, tahan terhadap hama dan penyakit, atau makin tinggi toleransinya terhadap kondisi cekaman (stress) lingkungan, serta ke depan dikembangkan tanaman dengan kandungan nutrisi yang lebih baik. Menurut sebuah studi (Runge dan Ryan 2004), sebanyak 63 negara telah dan sedang melaksanakan litbang tanaman biotek yang meliputi 57 jenis tanaman. Lebih dari separuh jumlah kegiatan litbang tersebut berada di negara berkembang, sekalipun dengan fokus dan prioritas yang berbeda-beda. Akan tetapi, laporan FAO (2004) baru-baru ini mengemukakan bahwa penelitian tanaman transgenik toleran cekaman abiotik (misalnya kekeringan dan salinitas) sangatlah sedikit. Kekeringan dan salinitas merupakan dua faktor pembatas utama pada lahan-lahan marjinal di negara-negara sedang berkembang. Padahal lahan marjinal dengan cekaman abiotik, yang dapat menurunkan hasil produksi atau membatasi realisasi potensi hasil, sangatlah luas. Untuk Indonesia luasnya mungkin mencapai puluhan juta hektar, terlebih lagi kalau mencakup faktor pembatas kemasaman tanah akibat kandungan aluminium, sulfat masam dan asam-asam organik yang tinggi seperti misalnya di lahan gambut. Suatu hal yang menarik bahwa dalam forum seminar ini secara khusus dibahas tema pemanfaatan bioteknologi untuk mengatasi cekaman abiotik. Menurut hemat saya, subyek ini termasuk strategis dan penting bagi Indonesia. Dalam makalah ini disajikan suatu telaah prospek dan tantangan yang dihadapi baik dengan belajar dari pengalaman komersialisasi tanaman biotek di Indonesia maupun dengan perspektif yang lebih luas. Juga didiskusikan seputar aspek manajemen dan strategi pengembangan produk, dan aspek regulasi dan iklim usaha. Diskusi tentang prospek suatu teknologi atau produk teknologi tentu tidak terlepas dari potensi aplikasinya.},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_52-60.pdf}
    }
  6. Pancoro, Adi. 2006. Functional Genomic Approaches to Plant Stress Resistance. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 61-67.
    [BibTeX] [Abstract] [PDF: Functional Genomic Approaches to Plant Stress Resistance ]
    Abiotic stresses, such as drought, salinity, extreme temperature, chemical toxicity and oxidative stress are serious threat to agriculture and the natural status of the environment 2. Study of abiotic stresses should be given high research priority in plant biotechnology 3. Molecular control mechanisms for abiotic stress tolerance are based on the activation dan regulation of spesific stress-related genes 4. Molecular mapping and genomics approaches offer new opportunities and strategies to dissect major genes and quantitative trait loci (QTL) underlying abiotic (drought) tolerance. 5. New molecular tools are available that can be integrated with conventional breeding and physiology to accelerate a basic understanding of drought tolerance in plants and the development of drought tolerance crops.
    @article{Pancoro06p61,
    title = {{Functional Genomic Approaches to Plant Stress Resistance}},
    author = {Adi Pancoro},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {61-67},
    year = {2006},
    abstract = {Abiotic stresses, such as drought, salinity, extreme temperature, chemical toxicity and oxidative stress are serious threat to agriculture and the natural status of the environment 2. Study of abiotic stresses should be given high research priority in plant biotechnology 3. Molecular control mechanisms for abiotic stress tolerance are based on the activation dan regulation of spesific stress-related genes 4. Molecular mapping and genomics approaches offer new opportunities and strategies to dissect major genes and quantitative trait loci (QTL) underlying abiotic (drought) tolerance. 5. New molecular tools are available that can be integrated with conventional breeding and physiology to accelerate a basic understanding of drought tolerance in plants and the development of drought tolerance crops.},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_61-67.pdf}
    }
  7. Takahashi, M.. 2006. Photosynthesis-related Stress on Plants and Their Resistance to It. Prosiding seminar nasional pemanfaatan bioteknologi untuk mengatasi cekaman abiotik pada tanaman 68-77.
    [BibTeX] [PDF: Photosynthesis-related Stress on Plants and Their Resistance to It ]
    @article{Takahashi06p68,
    title = {{Photosynthesis-related Stress on Plants and Their Resistance to It}},
    author = {M. Takahashi},
    journal = {Prosiding Seminar Nasional Pemanfaatan Bioteknologi untuk Mengatasi Cekaman Abiotik pada Tanaman},
    pages = {68-77},
    year = {2006},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2006_68-77.pdf}
    }

2004

  1. Dewi, Nurwita and Muhammad Sabda. 2004. Pelestarian In Vitro pada Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas. Prosiding seminar hasil penelitian bb-biogen tahun 2004 1-7.
    [BibTeX] [Abstract] [PDF: Pelestarian In Vitro pada Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas ]
    Conservation method of root crop germplasms such as taro, sweet potato, and cassava is usually done by conventional method. The crops are planted in the field along the year. This method needs a large area and has a high risk caused by environmental stress, biotic, and abiotic. In vitro conservation method is more beneficial because it needs relatively narrow space and the material is free from pest and disease. The objective of the activities was to add and maintain in vitro collection of taro, sweet potato, and cassava. In 2004, from the activities was obtained 1 number accession of taro, 50 number accession of sweet potato and 20 number accession of cassava as new in vitro collection. Maintain of culture collection was done by subculture. Acclimation after conservation showed that the culture have good regeneration ability to back to normal growth.
    @article{NurwitaDewi04p1,
    title = {{Pelestarian In Vitro pada Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas}},
    author = {Nurwita Dewi and Muhammad Sabda},
    journal = {Prosiding Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {1-7},
    year = {2004},
    abstract = {Conservation method of root crop germplasms such as taro, sweet potato, and cassava is usually done by conventional method. The crops are planted in the field along the year. This method needs a large area and has a high risk caused by environmental stress, biotic, and abiotic. In vitro conservation method is more beneficial because it needs relatively narrow space and the material is free from pest and disease. The objective of the activities was to add and maintain in vitro collection of taro, sweet potato, and cassava. In 2004, from the activities was obtained 1 number accession of taro, 50 number accession of sweet potato and 20 number accession of cassava as new in vitro collection. Maintain of culture collection was done by subculture. Acclimation after conservation showed that the culture have good regeneration ability to back to normal growth.},
    keywords = {in vitro, conservation, taro, sweet potato, cassava},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_1-7.pdf}
    }
  2. Rais, S. A., T. S. Sudiaty, S. G. Budiarti, N. Zuraida, I. H. Somantri, Hadiatmi, N. Dewi, T. Suhartini, and M. Setyowati. 2004. Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 8-34.
    [BibTeX] [Abstract] [PDF: Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan ]
    The objectives of the experiment was conserving the sustainability and characterizing the morphology of food crops. The experiment were conducted in the dry and rainy season on 2004. A total of 5569 accs germplasm of food crops had rejuvenation were conducted at several research installation such as in Citayam, Cikeumeuh, Pacet, Pusakanegara, and RK BB-Biogen. The result showed. That the rejuvenation of rice produced seed 10-1000 g/5 m2; seed of wild rice 57,5-732,6 g; seed of wheat 0,90-593, 6 g; seed of groundnuts 110-1500 g/3 m2; seed of mungbean 3,35-17,07 g; cowpea 100-500 g, and sorghum 207-780 g/10 panicle. Ten of rice have high yield >520 g/5 m2; Ten accs of rice have high tillering >21/plant, 10 accs unfilled grain <4,89%. Produced seed of corn from sibbing have 1 kg in ten germplasm on corn, and local Karang Asem no. regist 13.3681 of soybean, have a high of pods (101 pods), and local Tulung Agung have produced of yield 732,6 g/3 m2. 12 accs of cassava have high weight 3,0 kg/plant ei, V. Yorong, No. 520-41 and 547-9-10 have weight 4-4,5 kg/plant. 10 accs of sweet potatos have product of tuber 1300-1700 g/plant e.i : Mantang Biru, Helalekue Lama A, Ulupuklon, and Kiruluk have high of tuber 1500-1700 g/plant 5 accs of wheat e.i. V. Madona, S.W Puatro, Anemos, Sigma, and V235 have high tillering 7-8/plant and V. perdix have high yield 593,6 g/plot; 3 accs of mungbean e.i; VR176, VR178, VR151, have early maturity 58 days, 6 accs have a high number of pod 39-65/plant and 15 accs have seed of yield 12,07-17,03 g; 29 accs of cowpea have high grain yield/plant >500 g. 40 accs of groundnut have a high weight of day pod <700 g/3 m2, and 44 accs have pod/plant more than 15 pods. Variety B) (600) of taro have a tuber weight 1680 g/plant and tuber of balitung variety 29-BLT-ungu have high tillering tuber 57, variety 26-MLG-364; Variety 56 balitung and 35-MLG392 have a weight tuber <4250 g/plant. 5 accs germplasm of sorghum have early maturity <80 day.Variety ICSV91014 have panicle length, and 15 accs have ground weight 701-800 g/10 panicle.
    @article{Rais04p8,
    title = {{Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan}},
    author = {S. A. Rais and T. S. Sudiaty and S. G. Budiarti and N. Zuraida and I. H. Somantri and Hadiatmi and N. Dewi and T. Suhartini and M. Setyowati},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {8-34},
    year = {2004},
    abstract = {The objectives of the experiment was conserving the sustainability and characterizing the morphology of food crops. The experiment were conducted in the dry and rainy season on 2004. A total of 5569 accs germplasm of food crops had rejuvenation were conducted at several research installation such as in Citayam, Cikeumeuh, Pacet, Pusakanegara, and RK BB-Biogen. The result showed. That the rejuvenation of rice produced seed 10-1000 g/5 m2; seed of wild rice 57,5-732,6 g; seed of wheat 0,90-593, 6 g; seed of groundnuts 110-1500 g/3 m2; seed of mungbean 3,35-17,07 g; cowpea 100-500 g, and sorghum 207-780 g/10 panicle. Ten of rice have high yield >520 g/5 m2; Ten accs of rice have high tillering >21/plant, 10 accs unfilled grain <4,89%. Produced seed of corn from sibbing have 1 kg in ten germplasm on corn, and local Karang Asem no. regist 13.3681 of soybean, have a high of pods (101 pods), and local Tulung Agung have produced of yield 732,6 g/3 m2. 12 accs of cassava have high weight 3,0 kg/plant ei, V. Yorong, No. 520-41 and 547-9-10 have weight 4-4,5 kg/plant. 10 accs of sweet potatos have product of tuber 1300-1700 g/plant e.i : Mantang Biru, Helalekue Lama A, Ulupuklon, and Kiruluk have high of tuber 1500-1700 g/plant 5 accs of wheat e.i. V. Madona, S.W Puatro, Anemos, Sigma, and V235 have high tillering 7-8/plant and V. perdix have high yield 593,6 g/plot; 3 accs of mungbean e.i; VR176, VR178, VR151, have early maturity 58 days, 6 accs have a high number of pod 39-65/plant and 15 accs have seed of yield 12,07-17,03 g; 29 accs of cowpea have high grain yield/plant >500 g. 40 accs of groundnut have a high weight of day pod <700 g/3 m2, and 44 accs have pod/plant more than 15 pods. Variety B) (600) of taro have a tuber weight 1680 g/plant and tuber of balitung variety 29-BLT-ungu have high tillering tuber 57, variety 26-MLG-364; Variety 56 balitung and 35-MLG392 have a weight tuber <4250 g/plant. 5 accs germplasm of sorghum have early maturity <80 day.Variety ICSV91014 have panicle length, and 15 accs have ground weight 701-800 g/10 panicle.},
    keywords = {Germplasm, rejuvenation, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_8-34.pdf}
    }
  3. Suhartini, T., S. G. Budiarti, N. Zuraida, Hadiatmi, S. A. Rais, T. S. Silitonga, and N. Dewi. 2004. Karakterisasi Mutu Gizi Plasma Nutfah Tanaman Pangan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 35-50.
    [BibTeX] [Abstract] [PDF: Karakterisasi Mutu Gizi Plasma Nutfah Tanaman Pangan ]
    The objectives of the experiment was conserving the sustainability of food crops germplasm collection and to characterizing the nutritional characters. The experiment was conducted in: Lab. Quality BB-Biogen and Lab. Pasca Panen BB-Litbang Pasca Panen Pertanian Bogor. The results show that nutritional characterization of 8 accs. of corn with high amylose content 28.1-29.94% and 2 accs low amylose 13.7-18%. From 100 accs of rice 61 accs of amilose content had been retested in the Lab Biochemistry and Enzimatik BB-Biogen, it gained that variety of rice amilose content between 7.8-27.4%. There were 10 rice accs of low amilose content 7.73-19.54% and 23 accs of high amilose content 25.40-27.40% About 10 accs. In sweet potatoes there were 11 accs of high starch content >30% (30.45-35.76%) and 6 accs of low starch content 15.53-23.11%. The 4 accs. of ground nut with high protein content (28-30.30,74%)), and 10 accs. of ground nut with fat content varied 46.1-49,25%. Amount of 9 accs. of soybean with high protein content (46.67-40.03%) and fat content of soybean varied 21.48-16.54%. The 14 accs. of sorghum with low tanin 0.01-0.16% and 6 accs with high tanin content (0.77-0. 44%). The total of 30 accs. of cassava commonly with low HCN content (<20 ppm), and starch content have ranged 28.71% to 42.24%, the lowest of HCN content were 6 accs. (3-7 ppm) and 5 accs. have high starch content (40,25-42.24%). In Ganyong (Canna edulis (Ker.) about 4 accs. have high starch content (>30%) and 1 accs. have lowest starch content (19,46%).
    @article{Suhartini04p35,
    title = {{Karakterisasi Mutu Gizi Plasma Nutfah Tanaman Pangan}},
    author = {T. Suhartini and S. G. Budiarti and N. Zuraida and Hadiatmi and S. A. Rais and T. S. Silitonga and N. Dewi},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {35-50},
    year = {2004},
    abstract = {The objectives of the experiment was conserving the sustainability of food crops germplasm collection and to characterizing the nutritional characters. The experiment was conducted in: Lab. Quality BB-Biogen and Lab. Pasca Panen BB-Litbang Pasca Panen Pertanian Bogor. The results show that nutritional characterization of 8 accs. of corn with high amylose content 28.1-29.94% and 2 accs low amylose 13.7-18%. From 100 accs of rice 61 accs of amilose content had been retested in the Lab Biochemistry and Enzimatik BB-Biogen, it gained that variety of rice amilose content between 7.8-27.4%. There were 10 rice accs of low amilose content 7.73-19.54% and 23 accs of high amilose content 25.40-27.40% About 10 accs. In sweet potatoes there were 11 accs of high starch content >30% (30.45-35.76%) and 6 accs of low starch content 15.53-23.11%. The 4 accs. of ground nut with high protein content (28-30.30,74%)), and 10 accs. of ground nut with fat content varied 46.1-49,25%. Amount of 9 accs. of soybean with high protein content (46.67-40.03%) and fat content of soybean varied 21.48-16.54%. The 14 accs. of sorghum with low tanin 0.01-0.16% and 6 accs with high tanin content (0.77-0. 44%). The total of 30 accs. of cassava commonly with low HCN content (<20 ppm), and starch content have ranged 28.71% to 42.24%, the lowest of HCN content were 6 accs. (3-7 ppm) and 5 accs. have high starch content (40,25-42.24%). In Ganyong (Canna edulis (Ker.) about 4 accs. have high starch content (>30%) and 1 accs. have lowest starch content (19,46%).},
    keywords = {food crops germplasm, nutrition quality, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_35-50.pdf}
    }
  4. Budiarti, S. G., T. S. Silitonga, T. Suhartini, Sutoro, Asadi, and Hadiatmi. 2004. Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah/Lahan Masam (Keracunan Al Dan Fe) dan Pemupukan Rendah. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 51-61.
    [BibTeX] [Abstract] [PDF: Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah/Lahan Masam (Keracunan Al Dan Fe) dan Pemupukan Rendah ]
    The aim of these experiment were to find of rice genotipes that tolerant to Al and Fe toxicity, to find of maize and soybean tolerant to Al toxicity, and to find of maize genotipes that tolerant to low fertilizer. The activities that included evaluation for tolerance to Al and Fe toxicity conducted at Tamanbogo experiment station, Lampung, WS 2004, whereas evaluation of maize germplasm for tolerance to low fertilizer have been done at Cikeumeuh experiment farm, Bogor, WS 2004. The total of accessions that testing for Al toxicity were: 200 accession of rice, 100 accession of maize, and 100 accession of soybean. There were 100 accession of rice tested to Fe toxicity. The experiment were arranged randomized complete block design, 3 replications, except soybean (2 replications).Test to low fertilizer for maize using 100 accession with augmented design, 4 replications. The results showed that testing for Al toxicity found: 15 accession of rice tolerant (score 1-3), with 3 accession have score 1: Padi Juwa (Reg. 19732), Melaya (Reg. 19736), and Sibau (Reg. 19780); 10 accession of maize tolerant-moderate (score 2-3), 2 accession have yield >tolerant control (Sukmaraga) there were DMR Conv Tzi 1787 x KU 1414(Reg,3467) and Sidanak (Reg. 3573); 16 accession of soybean tolerant-moderate (score 2-3) there were 2 accession have the highest yield (Lokal Pasuruan and Sibayak). There were 24 accession of rice tolerant-moderate to Fe toxicity (score 1-3-5), and one of local accession (Lokal Sampit/Reg. 3982) showed the best vegetative growth (score 1). Evaluation of maize germplasm to low fertilizer found 16 accession (local varieties and introduction ) have yield >from average yield (1412 kg/ha), but still <from Bisma (3536 kg/ha ).
    @article{Budiarti04p51,
    title = {{Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah/Lahan Masam (Keracunan Al Dan Fe) dan Pemupukan Rendah}},
    author = {S. G. Budiarti and T. S. Silitonga and T. Suhartini and Sutoro and Asadi and Hadiatmi},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {51-61},
    year = {2004},
    abstract = {The aim of these experiment were to find of rice genotipes that tolerant to Al and Fe toxicity, to find of maize and soybean tolerant to Al toxicity, and to find of maize genotipes that tolerant to low fertilizer. The activities that included evaluation for tolerance to Al and Fe toxicity conducted at Tamanbogo experiment station, Lampung, WS 2004, whereas evaluation of maize germplasm for tolerance to low fertilizer have been done at Cikeumeuh experiment farm, Bogor, WS 2004. The total of accessions that testing for Al toxicity were: 200 accession of rice, 100 accession of maize, and 100 accession of soybean. There were 100 accession of rice tested to Fe toxicity. The experiment were arranged randomized complete block design, 3 replications, except soybean (2 replications).Test to low fertilizer for maize using 100 accession with augmented design, 4 replications. The results showed that testing for Al toxicity found: 15 accession of rice tolerant (score 1-3), with 3 accession have score 1: Padi Juwa (Reg. 19732), Melaya (Reg. 19736), and Sibau (Reg. 19780); 10 accession of maize tolerant-moderate (score 2-3), 2 accession have yield >tolerant control (Sukmaraga) there were DMR Conv Tzi 1787 x KU 1414(Reg,3467) and Sidanak (Reg. 3573); 16 accession of soybean tolerant-moderate (score 2-3) there were 2 accession have the highest yield (Lokal Pasuruan and Sibayak). There were 24 accession of rice tolerant-moderate to Fe toxicity (score 1-3-5), and one of local accession (Lokal Sampit/Reg. 3982) showed the best vegetative growth (score 1). Evaluation of maize germplasm to low fertilizer found 16 accession (local varieties and introduction ) have yield >from average yield (1412 kg/ha), but still <from Bisma (3536 kg/ha ).},
    keywords = {evaluation, al and fe toxicity, low fertilizer, rice, maize, soybean},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_51-61.pdf}
    }
  5. Zuraida, N., T. S. Silitonga, Suyono, Minantyorini, and D. Koswanudin. 2004. Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar). Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 62-66.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar) ]
    Evaluation of resistance to brown planthopper on rice germplasm were done in green house and evaluation of resistance to weevil on sweetpotato were done in Bank Gene Laboratory. Three hundreed accessions of rice germplasm were infested by 2-3 instar larvae of IR42 and IR64 populations with 3-4 bugs/plant, respectively at seven days old-plant. Evaluation on its resistance were scored after IR42 as a control showed 90% died. The total of fifty accessions of sweetpotato germplasm were infested by 5 pairs of Cylas formicarius, respectively on tuber root. Evaluation were done after 30 days of infestation. The result showed that one accession of rice germplasm had resistance to rice brownplanthopper of IR42 population and one accession had resistance to rice brown planthopper of IR64 population. No accession of sweetpotato germplasm had resistance to sweetpotato weevil.
    @article{Zuraida04p62,
    title = {{Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar)}},
    author = {N. Zuraida and T. S. Silitonga and Suyono and Minantyorini and D. Koswanudin},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {62-66},
    year = {2004},
    abstract = {Evaluation of resistance to brown planthopper on rice germplasm were done in green house and evaluation of resistance to weevil on sweetpotato were done in Bank Gene Laboratory. Three hundreed accessions of rice germplasm were infested by 2-3 instar larvae of IR42 and IR64 populations with 3-4 bugs/plant, respectively at seven days old-plant. Evaluation on its resistance were scored after IR42 as a control showed 90% died. The total of fifty accessions of sweetpotato germplasm were infested by 5 pairs of Cylas formicarius, respectively on tuber root. Evaluation were done after 30 days of infestation. The result showed that one accession of rice germplasm had resistance to rice brownplanthopper of IR42 population and one accession had resistance to rice brown planthopper of IR64 population. No accession of sweetpotato germplasm had resistance to sweetpotato weevil.},
    keywords = {rice, sweetpotato, germplasm, brown planthopper, weevil},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_62-66.pdf}
    }
  6. Hadiatm, Tiur S. Silitonga, Sri A. Rais, and Sri G. Budiarti. 2004. Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Plasma Nutfah Jagung terhadap Penyakit Bulai. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 67-73.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Plasma Nutfah Jagung terhadap Penyakit Bulai ]
    Tujuan penelitian ini adalah untuk memperoleh informasi ketahanan dari plasma nutfah padi ter-hadap penyakit HDB dan blas, dan plasma nutfah jagung terhadap penyakit bulai. Perlakuan terdiri dari 150 aksesi padi untuk pengujian HDB, 250 aksesi padi untuk pengujian penyakit blas, dan 100 aksesi jagung untuk penyakit bulai. Percobaan menggunakan rancangan acak kelompok dengan 2-3 ulangan. Percobaan dilaksanakan pada MH 2004 di Inlitbio Muara (uji penyakit HDB), di Sukabumi (uji penyakit blas) dan Inlitbio Cikeumeuh (uji penyakit bulai). Dari hasil penelitian diper-oleh 11 aksesi plasma nutfah padi yang tahan terhadap penyakit HDB kelompok IV, 5 aksesi tahan terhadap penyakit HDB IV dan VIII, yaitu Sate Liko (R. 10077), Pulu Bolong (R. 10221), Pulut Tomene (R. 10578), Daya Itoh Rice (R. 12074), dan Horeg (R. 15016). Diperoleh 6 aksesi padi ter-hadap penyakit blas daun dan satu aksesi yang tahan terhadap penyakit blas leher, yaitu IR2031-522-6-9 (R. 19167). Sebanyak 30 aksesi jagung sangat tahan terhadap penyakit bulai (intensitas serangan 0-5,0%) dan 17 aksesi bereaksi tahan (intensitas serangan 6-10%).
    @article{Hadiatm04p67,
    title = {{Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Plasma Nutfah Jagung terhadap Penyakit Bulai}},
    author = {Hadiatm and Tiur S. Silitonga and Sri A. Rais and Sri G. Budiarti},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {67-73},
    year = {2004},
    abstract = {Tujuan penelitian ini adalah untuk memperoleh informasi ketahanan dari plasma nutfah padi ter-hadap penyakit HDB dan blas, dan plasma nutfah jagung terhadap penyakit bulai. Perlakuan terdiri dari 150 aksesi padi untuk pengujian HDB, 250 aksesi padi untuk pengujian penyakit blas, dan 100 aksesi jagung untuk penyakit bulai. Percobaan menggunakan rancangan acak kelompok dengan 2-3 ulangan. Percobaan dilaksanakan pada MH 2004 di Inlitbio Muara (uji penyakit HDB), di Sukabumi (uji penyakit blas) dan Inlitbio Cikeumeuh (uji penyakit bulai). Dari hasil penelitian diper-oleh 11 aksesi plasma nutfah padi yang tahan terhadap penyakit HDB kelompok IV, 5 aksesi tahan terhadap penyakit HDB IV dan VIII, yaitu Sate Liko (R. 10077), Pulu Bolong (R. 10221), Pulut Tomene (R. 10578), Daya Itoh Rice (R. 12074), dan Horeg (R. 15016). Diperoleh 6 aksesi padi ter-hadap penyakit blas daun dan satu aksesi yang tahan terhadap penyakit blas leher, yaitu IR2031-522-6-9 (R. 19167). Sebanyak 30 aksesi jagung sangat tahan terhadap penyakit bulai (intensitas serangan 0-5,0%) dan 17 aksesi bereaksi tahan (intensitas serangan 6-10%).},
    keywords = {BLB, blas, bulai, plasma nutfah},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_67-73.pdf}
    }
  7. Kurniawan, H., Sutoro, M. Setyowati, T. S. Silitonga, S. G. Budiarti, Hadiatmi, Asadi, N. Dewi, S. A. Rais, I. H. Somantri, N. Zuraida, Minantyorini, and T. Suhartini. 2004. Pengembangan Sistem Pangkalan Data (Database) Plasma Nutfah Tanaman Pangan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 74-84.
    [BibTeX] [Abstract] [PDF: Pengembangan Sistem Pangkalan Data (Database) Plasma Nutfah Tanaman Pangan ]
    Many activities are involve in food crops germplasm management such as exploration, registration of accessions, preservation, characterization, evaluation, and utilization of its accession(s) for breeding program. Each of these activities produce a lot of valuable data that have to be documented systematically. Consequently, a database system should be established to ensure the availability of data and information that can be easily, rapidly, and accurately accessed. Since 2000, the Microsoft Access-based database system has been developed for managing food crops germplasm data. Until February 2005, a total of 9423 records were managed, which is consisted of 3653 records of rice (41 descriptors), 734 records of corn (29 descriptors), 210 records of sorghum (22 descriptors), 771 records of soybean (28 descriptors), 619 records of groundnut (19 descriptors), 1024 records of mungbean (20 descriptors), 434 records of cassava (23 descriptors), 1426 records of sweetpotato (35 descriptors), 112 records of cowpea (22 descriptors), 34 records of Dioscorea alata (5 descriptors), 33 records of Dioscorea esculenta (5 descriptors), 48 records of Canna edulis (5 descriptors), 115 records of Colocasia esculenta, 58 records of Xanthossoma sp., 88 records of wildrice (5 descriptors), and 64 records of wheat (5 descriptors). All of recorded data were compiled as a food crops catalogue that will be updated annually. This catalogue book is expected to become a useful information resources for users. In addition, a database system on compact disc and internet being developed to enhance data accessibility on the network.
    @article{Kurniawan04p74,
    title = {{Pengembangan Sistem Pangkalan Data (Database) Plasma Nutfah Tanaman Pangan}},
    author = {H. Kurniawan and Sutoro and M. Setyowati and T. S. Silitonga and S. G. Budiarti and Hadiatmi and Asadi and N. Dewi and S. A. Rais and I. H. Somantri and N. Zuraida and Minantyorini and T. Suhartini},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {74-84},
    year = {2004},
    abstract = {Many activities are involve in food crops germplasm management such as exploration, registration of accessions, preservation, characterization, evaluation, and utilization of its accession(s) for breeding program. Each of these activities produce a lot of valuable data that have to be documented systematically. Consequently, a database system should be established to ensure the availability of data and information that can be easily, rapidly, and accurately accessed. Since 2000, the Microsoft Access-based database system has been developed for managing food crops germplasm data. Until February 2005, a total of 9423 records were managed, which is consisted of 3653 records of rice (41 descriptors), 734 records of corn (29 descriptors), 210 records of sorghum (22 descriptors), 771 records of soybean (28 descriptors), 619 records of groundnut (19 descriptors), 1024 records of mungbean (20 descriptors), 434 records of cassava (23 descriptors), 1426 records of sweetpotato (35 descriptors), 112 records of cowpea (22 descriptors), 34 records of Dioscorea alata (5 descriptors), 33 records of Dioscorea esculenta (5 descriptors), 48 records of Canna edulis (5 descriptors), 115 records of Colocasia esculenta, 58 records of Xanthossoma sp., 88 records of wildrice (5 descriptors), and 64 records of wheat (5 descriptors). All of recorded data were compiled as a food crops catalogue that will be updated annually. This catalogue book is expected to become a useful information resources for users. In addition, a database system on compact disc and internet being developed to enhance data accessibility on the network.},
    keywords = {database, germplasm, food crop},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_74-84.pdf}
    }
  8. Sutoro. 2004. Manajemen Benih Plasma Nutfah Tanaman Pangan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 85-114.
    [BibTeX] [Abstract] [PDF: Manajemen Benih Plasma Nutfah Tanaman Pangan ]
    Konservasi plasma nutfah tanaman berbiji dapat dilakukan secara ex situ dengan cara menyimpan benih dalam ruang dingin. Dalam rangka menjaga kelestarian plasma nutfah yang disimpan maka perlu dilakukan monitoring viabilitas benih secara periodik. Masalah yang dihadapi dalam penyim-panan benih dalam ruangan dingin, yaitu fasilitas listrik sering tidak kontinu sehingga akan meng-ganggu suhu dan kelembaban yang dapat mempengaruhi viabilitas benih yang disimpan. Berdasar-kan informasi viabilitas benih dari hasil monitoring tersebut maka dapat ditentukan aksesi-aksesi plasma nutfah yang perlu segera direjuvenasi kembali. Kegiatan uji daya tumbuh dilaksanakan di Laboratorium Bank Gen dan Genetika Tanaman BB-Biogen Bogor. Sistem database manajemen benih disusun dengan menggunakan software Microsoft-ACCESS. Hasil pengamatan daya tumbuh benih plasma nutfah dari hasil panen tampaknya banyak yang tidak memenuhi persyaratan untuk disimpan sebagai konservasi ex situ. Oleh karena itu, perlu segera direjuvenasi lagi. Selain faktor tempat penyimpanan benih, faktor penanganan prosesing perlu mendapat perhatian yang lebih intensif. Monitoring benih plasma nutfah perlu dilakukan secara terus menerus agar koleksi benih dapat dipertahankan dan dapat dilestarikan.
    @article{Sutoro04p85,
    title = {{Manajemen Benih Plasma Nutfah Tanaman Pangan}},
    author = {Sutoro},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {85-114},
    year = {2004},
    abstract = {Konservasi plasma nutfah tanaman berbiji dapat dilakukan secara ex situ dengan cara menyimpan benih dalam ruang dingin. Dalam rangka menjaga kelestarian plasma nutfah yang disimpan maka perlu dilakukan monitoring viabilitas benih secara periodik. Masalah yang dihadapi dalam penyim-panan benih dalam ruangan dingin, yaitu fasilitas listrik sering tidak kontinu sehingga akan meng-ganggu suhu dan kelembaban yang dapat mempengaruhi viabilitas benih yang disimpan. Berdasar-kan informasi viabilitas benih dari hasil monitoring tersebut maka dapat ditentukan aksesi-aksesi plasma nutfah yang perlu segera direjuvenasi kembali. Kegiatan uji daya tumbuh dilaksanakan di Laboratorium Bank Gen dan Genetika Tanaman BB-Biogen Bogor. Sistem database manajemen benih disusun dengan menggunakan software Microsoft-ACCESS. Hasil pengamatan daya tumbuh benih plasma nutfah dari hasil panen tampaknya banyak yang tidak memenuhi persyaratan untuk disimpan sebagai konservasi ex situ. Oleh karena itu, perlu segera direjuvenasi lagi. Selain faktor tempat penyimpanan benih, faktor penanganan prosesing perlu mendapat perhatian yang lebih intensif. Monitoring benih plasma nutfah perlu dilakukan secara terus menerus agar koleksi benih dapat dipertahankan dan dapat dilestarikan.},
    keywords = {Plasma nutfah, daya tumbuh, monitoring},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_85-114.pdf}
    }
  9. Ambarwati, A. D., I. Hanarida, A. Apriana, T. J. Santoso, I. S. Dewi, A. Sisharmini, and I. M. Samudra. 2004. Perakitan Tanaman Padi Transgenik untuk Ketahanan terhadap Hama Penggerek Batang. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 115-123.
    [BibTeX] [Abstract] [PDF: Perakitan Tanaman Padi Transgenik untuk Ketahanan terhadap Hama Penggerek Batang ]
    Rice stemborer (Scirpophaga sp.) is considered as one of primary pests causing yield decrease in Indonesia. Conventional breeding for stemborer resistance in rice has difficulty, due to the sources of resistance gene is lacking in rice germplasm. Genetic engineering on the other hand, offers a means to introduce into rice genom, genes which confer increased tolerance to pests. This continuation experiment consist of 3 activities, i.e: molecular analysis of putative transgenic rice of T-309 T5 generation, bioassay of putative transgenic rice T-309 T5 generation, and molecular analysis and bioassay of putative transgenic rice T-309 T4 generation. Molecular analysis to corfirm the existence of cryIA(b) gene have been conducted through PCR technique while for detecting the expression of Bt- cryIA(b) protein conducted using a immunostrip testing. Out of 165 T5 generation transgenic putative rice plants have been gained 85 plants (51.1%) containing the cryIA(b) gene using PCR testing and 50 plants (30.0%) expressed a low concentration of cryIA(b) protein using the immunostrip testing. Meanwhile, Out of 83 T4 generation transgenic putative rice plants have been gained 38 plants (45,8%) containing the cryIA(b) gene using PCR testing and 31 plants (37,4%) expressed the a low concentration of cryIA(b) protein using the immunostrip testing. Result of in planta bioassay showed that level of resistance to rice stem borer was varied among T4 and T5 generation. Among 50 of T5 -generation of putative T-309 transgenic rice plants only 13 plants were highly resistance to stem borer either in vegetative (deadheart symptoms) or generative (whitehead symptoms) stage of growth. They were 4 plants of T5-93 (2, 3, 4, and 5), 3 plants of T5-95 (2, 3, and 9), 5 plants of T5-103 (4, 8, 12, 14, and 15), 1 plant of T5-105-3 and 1 plat of T5-112-1. Meanwhile, only 1 plant categorized as resistance to stem borer, i.e. T5-73-6. Among 31 of T4 -generation of putative T-309 transgenic rice plants only 8 plants were highly resistance to stem borer either in vegetative or generative stage of growth. They were 5 plants of T4-1 (5, 16, 21, 23, and 24), 1 plant of T4-2-12, 1 plants of T4-3-1, dan 1 plant of T4-4-10. Meanwhile, plants categorized as resistance to stemborer were 9 plants, i.e 1 plant of T4-1-12, 2 plants of T4-2 (15 dan 18), 1 plant of T4-3-8 and 5 plant of T4-4 (13, 16, 17, 22, and 30).
    @article{Ambarwati04p115,
    title = {{Perakitan Tanaman Padi Transgenik untuk Ketahanan terhadap Hama Penggerek Batang}},
    author = {A. D. Ambarwati and I. Hanarida and A. Apriana and T. J. Santoso and I. S. Dewi and A. Sisharmini and I. M. Samudra},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {115-123},
    year = {2004},
    abstract = {Rice stemborer (Scirpophaga sp.) is considered as one of primary pests causing yield decrease in Indonesia. Conventional breeding for stemborer resistance in rice has difficulty, due to the sources of resistance gene is lacking in rice germplasm. Genetic engineering on the other hand, offers a means to introduce into rice genom, genes which confer increased tolerance to pests. This continuation experiment consist of 3 activities, i.e: molecular analysis of putative transgenic rice of T-309 T5 generation, bioassay of putative transgenic rice T-309 T5 generation, and molecular analysis and bioassay of putative transgenic rice T-309 T4 generation. Molecular analysis to corfirm the existence of cryIA(b) gene have been conducted through PCR technique while for detecting the expression of Bt- cryIA(b) protein conducted using a immunostrip testing. Out of 165 T5 generation transgenic putative rice plants have been gained 85 plants (51.1%) containing the cryIA(b) gene using PCR testing and 50 plants (30.0%) expressed a low concentration of cryIA(b) protein using the immunostrip testing. Meanwhile, Out of 83 T4 generation transgenic putative rice plants have been gained 38 plants (45,8%) containing the cryIA(b) gene using PCR testing and 31 plants (37,4%) expressed the a low concentration of cryIA(b) protein using the immunostrip testing. Result of in planta bioassay showed that level of resistance to rice stem borer was varied among T4 and T5 generation. Among 50 of T5 -generation of putative T-309 transgenic rice plants only 13 plants were highly resistance to stem borer either in vegetative (deadheart symptoms) or generative (whitehead symptoms) stage of growth. They were 4 plants of T5-93 (2, 3, 4, and 5), 3 plants of T5-95 (2, 3, and 9), 5 plants of T5-103 (4, 8, 12, 14, and 15), 1 plant of T5-105-3 and 1 plat of T5-112-1. Meanwhile, only 1 plant categorized as resistance to stem borer, i.e. T5-73-6. Among 31 of T4 -generation of putative T-309 transgenic rice plants only 8 plants were highly resistance to stem borer either in vegetative or generative stage of growth. They were 5 plants of T4-1 (5, 16, 21, 23, and 24), 1 plant of T4-2-12, 1 plants of T4-3-1, dan 1 plant of T4-4-10. Meanwhile, plants categorized as resistance to stemborer were 9 plants, i.e 1 plant of T4-1-12, 2 plants of T4-2 (15 dan 18), 1 plant of T4-3-8 and 5 plant of T4-4 (13, 16, 17, 22, and 30).},
    keywords = {Transgenic rice, cryIA(b) gene, molecular analysis, bioassay, rice stem borer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_115-123.pdf}
    }
  10. Listanto, E., H. Rizjaani, Liberty, T. J. Santoso, S. J. Pardal, I. H. Somantri, I. Altosar, and M. Herman. 2004. Konstruksi dan Kloning Plasmid pCambia1301 dengan Gen Cry1Ab. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 124-131.
    [BibTeX] [Abstract] [PDF: Konstruksi dan Kloning Plasmid pCambia1301 dengan Gen Cry1Ab ]
    Penelitian konstruksi dan kloning plasmid pCambia1301 dengan gen cry1Ab telah dilakukan di Laboratorium Biologi Molekuler, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor. Penelitian ini bertujuan untuk mendapatkan konstruksi plasmid pCambia yang mengandung gen cry1Ab. Proses konstruksi dilakukan dengan penyisipan gen cry1Ab pada vektor pCambia1301 menggunakan proses ligasi. Ligan ditransformasi ke dalam Escherichia coliDH5$\alpha$ dengan metode kejutan panas dan ke dalam Agrobacterium tumefaciens LBA4404 dengan metode kejutan dingin atau tri- parental mating. Hasil ligasi dan transformasi ke dalam bakteri diperoleh dua DNA plasmid dari dua koloni berbeda. DNA plasmid rekombinan terse-but sebagai pC1Ab dengan ukuran 14,8 kb berdasarkan uji pola restriksi dengan enzim HindIII yang membentuk dua fragmen DNA berukuran 11,8 kb dan 3,0 kb. Uji pola restriksi dengan enzim HindIII dan EcoRI diperoleh 3 fragmen DNA berukuran 11,8 kb, 2,7 kb, dan 0,3 kb. Uji pola restriksi de-ngan BamHI dan HindIII diperoleh 3 fragmen DNA berukuran 11,8 kb, 2,1 kb, dan 0,8 kb. Sedang-kan, pemotongan dengan enzim EcoRI menunjukkan dua fragmen DNA berukuran 14,5 kb dan 0,3 kb dari kedua DNA plasmid pC1Ab yang menunjukkan arah yang sama, yaitu ke kiri. Hasil uji PCR dari plasmid pC1Ab diperoleh fragmen gen cry1Ab berukuran 1,0 kb. Hasil uji GUS terhadap plas-mid pC1Ab yang ditrasformasikan ke eksplan kotiledon melalui A. tumefaciens menunjukkan reaksi GUS positip berwarna biru.
    @article{Listanto04p124,
    title = {{Konstruksi dan Kloning Plasmid pCambia1301 dengan Gen Cry1Ab}},
    author = {E. Listanto and H. Rizjaani and Liberty and T. J. Santoso and S. J. Pardal and I. H. Somantri and I. Altosar and M. Herman},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {124-131},
    year = {2004},
    abstract = {Penelitian konstruksi dan kloning plasmid pCambia1301 dengan gen cry1Ab telah dilakukan di Laboratorium Biologi Molekuler, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor. Penelitian ini bertujuan untuk mendapatkan konstruksi plasmid pCambia yang mengandung gen cry1Ab. Proses konstruksi dilakukan dengan penyisipan gen cry1Ab pada vektor pCambia1301 menggunakan proses ligasi. Ligan ditransformasi ke dalam Escherichia coliDH5$\alpha$ dengan metode kejutan panas dan ke dalam Agrobacterium tumefaciens LBA4404 dengan metode kejutan dingin atau tri- parental mating. Hasil ligasi dan transformasi ke dalam bakteri diperoleh dua DNA plasmid dari dua koloni berbeda. DNA plasmid rekombinan terse-but sebagai pC1Ab dengan ukuran 14,8 kb berdasarkan uji pola restriksi dengan enzim HindIII yang membentuk dua fragmen DNA berukuran 11,8 kb dan 3,0 kb. Uji pola restriksi dengan enzim HindIII dan EcoRI diperoleh 3 fragmen DNA berukuran 11,8 kb, 2,7 kb, dan 0,3 kb. Uji pola restriksi de-ngan BamHI dan HindIII diperoleh 3 fragmen DNA berukuran 11,8 kb, 2,1 kb, dan 0,8 kb. Sedang-kan, pemotongan dengan enzim EcoRI menunjukkan dua fragmen DNA berukuran 14,5 kb dan 0,3 kb dari kedua DNA plasmid pC1Ab yang menunjukkan arah yang sama, yaitu ke kiri. Hasil uji PCR dari plasmid pC1Ab diperoleh fragmen gen cry1Ab berukuran 1,0 kb. Hasil uji GUS terhadap plas-mid pC1Ab yang ditrasformasikan ke eksplan kotiledon melalui A. tumefaciens menunjukkan reaksi GUS positip berwarna biru.},
    keywords = {Plasmid biner, gen cry1Ab, Agrobacterium tumefaciens, uji pola restriksi},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_124-131.pdf}
    }
  11. Pardal, S. J., M. Herman, T. I. R. Utami, E. Listanto, B. Santosa, Slamet, I. Mariska, S. Hutami, and A. Husni. 2004. Insersi Gen cry1Ab pada Tanaman Kedelai melalui Penembakan Partikel. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 132-139.
    [BibTeX] [Abstract] [PDF: Insersi Gen cry1Ab pada Tanaman Kedelai melalui Penembakan Partikel ]
    Perakitan tanaman kedelai transgenik tahan hama penggerek polong menggunakan gen cry1Ab merupakan alternatif yang potensial dalam perbaikan tanaman. Gen tersebut dapat menghasilkan protein $\delta$-endotoksin yang bersifat racun (insektisidal) terhadap larva serangga hama Lepidoptera. Insersi gen cry1Ab menggunakan metode penembakan partikel telah dilakukan di Laboratorium Biologi Molekuler, BB-Biogen pada tahun 2004. Tujuan penelitian adalah untuk mendapatkan tanaman kedelai hasil transformasi yang mengandung insersi gen cry1Ab. Dua varietas kedelai, Sindoro dan Wilis telah digunakan dalam penelitian ini. Sebanyak 230 kalus yang yang berasal dari eksplan embrio muda kedelai Wilis telah ditembak dengan plasmid pSBB yang mengandung gen cry1Ab dan pRQ6 yang mengandung gen hph (ketahanan terhadap higromisin) secara ko-transfor-masi. Penembak gen (gene gun) yang digunakan adalah Biolistic PDS 1000 He dari Biorad. Hasil regenerasi dan seleksi kalus Wilis hasil transformasi pada media yang mengandung higromisin di-peroleh 28 embrio somatik yang kemudian tumbuh menjadi 5 planlet. Namun, kelima planlet ini gagal diaklimatisasikan. Kemudian sebanyak 810 kalus embrio Sindoro yang ditransformasi dengan plasmid dan metode yang sama menghasilkan 154 embrio somatik dan tumbuh menjadi 27 benih somatik (planlet). Planlet ini selanjutnya diaklimatisasi dan 2 di antaranya telah berhasil tumbuh menjadi tanaman. Analisis molekuler terhadap 22 kalus kedelai hasil transformasi dengan teknik PCR menggunakan primer spesifik menunjukkan 8 sampel positif mengandung gen cry1Ab. Kemu-dian analisis molekuler terhadap 5 sampel DNA daun dari planlet kedelai Sindoro hasil regenerasi menunjukkan positif mengandung gen cry1Ab.
    @article{Pardal04p132,
    title = {{Insersi Gen cry1Ab pada Tanaman Kedelai melalui Penembakan Partikel}},
    author = {S. J. Pardal and M. Herman and T. I. R. Utami and E. Listanto and B. Santosa and Slamet and I. Mariska and S. Hutami and A. Husni},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {132-139},
    year = {2004},
    abstract = {Perakitan tanaman kedelai transgenik tahan hama penggerek polong menggunakan gen cry1Ab merupakan alternatif yang potensial dalam perbaikan tanaman. Gen tersebut dapat menghasilkan protein $\delta$-endotoksin yang bersifat racun (insektisidal) terhadap larva serangga hama Lepidoptera. Insersi gen cry1Ab menggunakan metode penembakan partikel telah dilakukan di Laboratorium Biologi Molekuler, BB-Biogen pada tahun 2004. Tujuan penelitian adalah untuk mendapatkan tanaman kedelai hasil transformasi yang mengandung insersi gen cry1Ab. Dua varietas kedelai, Sindoro dan Wilis telah digunakan dalam penelitian ini. Sebanyak 230 kalus yang yang berasal dari eksplan embrio muda kedelai Wilis telah ditembak dengan plasmid pSBB yang mengandung gen cry1Ab dan pRQ6 yang mengandung gen hph (ketahanan terhadap higromisin) secara ko-transfor-masi. Penembak gen (gene gun) yang digunakan adalah Biolistic PDS 1000 He dari Biorad. Hasil regenerasi dan seleksi kalus Wilis hasil transformasi pada media yang mengandung higromisin di-peroleh 28 embrio somatik yang kemudian tumbuh menjadi 5 planlet. Namun, kelima planlet ini gagal diaklimatisasikan. Kemudian sebanyak 810 kalus embrio Sindoro yang ditransformasi dengan plasmid dan metode yang sama menghasilkan 154 embrio somatik dan tumbuh menjadi 27 benih somatik (planlet). Planlet ini selanjutnya diaklimatisasi dan 2 di antaranya telah berhasil tumbuh menjadi tanaman. Analisis molekuler terhadap 22 kalus kedelai hasil transformasi dengan teknik PCR menggunakan primer spesifik menunjukkan 8 sampel positif mengandung gen cry1Ab. Kemu-dian analisis molekuler terhadap 5 sampel DNA daun dari planlet kedelai Sindoro hasil regenerasi menunjukkan positif mengandung gen cry1Ab. },
    keywords = {insersi, gen cry1Ab, particle bombardment},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_132-139.pdf}
    }
  12. Septiningsih, E. M., T. J. Santoso, D. W. Utami, and N. Hidayatun. 2004. Analisis Sidik Jari DNA Varietas Tanaman Pangan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 140-151.
    [BibTeX] [Abstract] [PDF: Analisis Sidik Jari DNA Varietas Tanaman Pangan ]
    In the fiscal year of 2004, variety identification of a set of rice, sweet potato, and soybean accessions had been completed using fluorescently-labeled SSR (simple sequence repeat) markers on a capillary-electrophoresis DNA genetic analyzer. Based on this technology, DNA fragment sizes can be more accurately measured and the genotyping protocol can be automated in a high-throughput manner. Accuracy is an important issue for variety identification, especially for variety conformation, registration, and plant protection. In addition, germplasm as a whole can be further analyzed to measure the amount of the genetic diversity and to identify agronomically-important genes or alleles for variety improvement. The results of this study showed that nearly all of the accessions used have unique DNA fingerprints or genetic identities. The rare alleles (frequency <5%) that might have the potential in the variety improvement program efforts had been detected as well. The results of an analysis of 96 rice accessions based on 30 SSRs showed 3 distinct clusters, i.e. wild relatives, Japonica, and Indica groups. As many as 305 alleles, ranging between 4-22 alleles per locus, were detected. Additionally, alleles that were specific to certain groups or combinations of different groups were identified. The relatedness of some alleles to certain groups of germplasm could help us to decide which SSR markers will be useful to analyze populations derived from crosses between two parents. The average value of the gene diversity is 0.612, and the PIC (polymorphism information content) value is 0.581. The analysis of the 96 sweet potato accessions using 10 SSRs demonstrated that there was no association between accessions and their geographical origins, except the ones originating from Papua, which were represented by two regions, i.e. Wamena and Manokwari. Accessions that originated from Wamena have dominated a different cluster than those of Manokwari. There is a possibility of different founder groups combined with geographic isolation between the two regions causing increased genetic distance. Some accessions originating from these two regions have also been found in other different clusters. This phenomenon demonstrated the high level of genetic diversity of sweet potato germplasm originated from Papua. The results of this study also provided additional evidence that Papua is a secondary center for the world sweet potato genetic diversity. Lastly, the results of the soybean study using 96 accessions and 10 SSRs detected 116 alleles, ranging between 7-19 alleles per locus, with the PIC value of 0.703. There was a tendency for the improved varieties to cluster together in certain groups showing that there were close genetic relationships among them. In addition, molecular differences between 2 accessions having the same names but different numbers of registration were detected. Furthermore, 2 accessions having different names but the same molecular identity were identified.
    @article{Septiningsih04p140,
    title = {{Analisis Sidik Jari DNA Varietas Tanaman Pangan}},
    author = {E. M. Septiningsih and T. J. Santoso and D. W. Utami and N. Hidayatun},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {140-151},
    year = {2004},
    abstract = {In the fiscal year of 2004, variety identification of a set of rice, sweet potato, and soybean accessions had been completed using fluorescently-labeled SSR (simple sequence repeat) markers on a capillary-electrophoresis DNA genetic analyzer. Based on this technology, DNA fragment sizes can be more accurately measured and the genotyping protocol can be automated in a high-throughput manner. Accuracy is an important issue for variety identification, especially for variety conformation, registration, and plant protection. In addition, germplasm as a whole can be further analyzed to measure the amount of the genetic diversity and to identify agronomically-important genes or alleles for variety improvement. The results of this study showed that nearly all of the accessions used have unique DNA fingerprints or genetic identities. The rare alleles (frequency <5%) that might have the potential in the variety improvement program efforts had been detected as well. The results of an analysis of 96 rice accessions based on 30 SSRs showed 3 distinct clusters, i.e. wild relatives, Japonica, and Indica groups. As many as 305 alleles, ranging between 4-22 alleles per locus, were detected. Additionally, alleles that were specific to certain groups or combinations of different groups were identified. The relatedness of some alleles to certain groups of germplasm could help us to decide which SSR markers will be useful to analyze populations derived from crosses between two parents. The average value of the gene diversity is 0.612, and the PIC (polymorphism information content) value is 0.581. The analysis of the 96 sweet potato accessions using 10 SSRs demonstrated that there was no association between accessions and their geographical origins, except the ones originating from Papua, which were represented by two regions, i.e. Wamena and Manokwari. Accessions that originated from Wamena have dominated a different cluster than those of Manokwari. There is a possibility of different founder groups combined with geographic isolation between the two regions causing increased genetic distance. Some accessions originating from these two regions have also been found in other different clusters. This phenomenon demonstrated the high level of genetic diversity of sweet potato germplasm originated from Papua. The results of this study also provided additional evidence that Papua is a secondary center for the world sweet potato genetic diversity. Lastly, the results of the soybean study using 96 accessions and 10 SSRs detected 116 alleles, ranging between 7-19 alleles per locus, with the PIC value of 0.703. There was a tendency for the improved varieties to cluster together in certain groups showing that there were close genetic relationships among them. In addition, molecular differences between 2 accessions having the same names but different numbers of registration were detected. Furthermore, 2 accessions having different names but the same molecular identity were identified.},
    keywords = {Variety identity, genetic diversity, rice, sweet potato, soybean, fluorescently-labeled SSR, capillary electrophoresis},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_140-151.pdf}
    }
  13. Dewi, I. S., A. Apriana, A. Sisharmini, and I. H. Somantri. 2004. Evaluasi Tanaman Padi Haploid Ganda Calon Tetua Padi Hibrida terhadap Wereng Batang Coklat dan Hawar Daun Bakteri. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 152-158.
    [BibTeX] [Abstract] [PDF: Evaluasi Tanaman Padi Haploid Ganda Calon Tetua Padi Hibrida terhadap Wereng Batang Coklat dan Hawar Daun Bakteri ]
    The susceptibility of introduced hybrid rice to main rice pests and diseases, such as brown plant hopper (BHP) and bacterial leaf blight (BLB), is subject to be improved through the improvement of its parental lines, i.e. restorer and cms lines. From previous experiment, through anther culture of F1 from maintainer or restorer crossed to released varieties we obtained doubled haploid spontaneous plants or pure lines. The objectives of this research were to evaluate those doubled haploid plants to BHP and BLB. Standard Evalution System for Rice (SES, IRRI) were used to evaluate their resistance to those pest and disease. Plant materials used were a. 15 doubledhaploid plants (DH2) potential as cms lines derived from anther culture of F1 IR58025B x Sintanur (M1) and IR62829B x Ciherang (M2) and b. 17 doubled-haploid plants (DH2) potential as restorer lines derived from anther culture of F1 IR53942R x Ciherang (R1) and BR82735R x Sintanur (R2). All plant materials were exposed to BHP (biotype SU) and BLB straian IV and VIII. Bioassay with BHP biotype SU showed that for plants potential as maintainer lines their were 3 moderately susceptible lines and 12 susceptible lines, while for plants potential as restorer lines their were one resistance line (BioR-Ac-W-HD-15), one moderately resistance line (BioR-Ac-W-HD-16), 5 moderately susceptible lines and 10 susceptible lines. The result of bioassay with BLB strain IV and VIII in lines potential as maintainer were: a). For BLB strain IV we obtained one moderately resistance line (BioM-Ac-W-HD-4), while the rest (14 lines) were moderately susceptible, b). Forr BLB strain VIII we obtained 5 moderately resistance lines (BioM-Ac-W-HD-5,6,7,11 dan 12), while the other 10 lines were moderately susceptible. Furthermore, the result of bioassay with BLB strain IV and VIII in lines potential as restorer lines were: a). For BLB strain VI we obtained 2 moderately resistance lines (BioR-Ac-W-HD-15, and BioR-Ac-W-HD-16), while the other 15 lines were moderately susceptible, b). For BLB strain VIII we obtained 16 moderately resistance lines, i.e. BioR-Ac-W-HD-(1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, dan 17), while one line were moderately susceptible. All lines having resistance and moderately resistance to WBC biotype SU and/or HDB strain IV and VIII will be evaluated futher.
    @article{Dewi04p152,
    title = {{Evaluasi Tanaman Padi Haploid Ganda Calon Tetua Padi Hibrida terhadap Wereng Batang Coklat dan Hawar Daun Bakteri}},
    author = {I. S. Dewi and A. Apriana and A. Sisharmini and I. H. Somantri},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {152-158},
    year = {2004},
    abstract = {The susceptibility of introduced hybrid rice to main rice pests and diseases, such as brown plant hopper (BHP) and bacterial leaf blight (BLB), is subject to be improved through the improvement of its parental lines, i.e. restorer and cms lines. From previous experiment, through anther culture of F1 from maintainer or restorer crossed to released varieties we obtained doubled haploid spontaneous plants or pure lines. The objectives of this research were to evaluate those doubled haploid plants to BHP and BLB. Standard Evalution System for Rice (SES, IRRI) were used to evaluate their resistance to those pest and disease. Plant materials used were a. 15 doubledhaploid plants (DH2) potential as cms lines derived from anther culture of F1 IR58025B x Sintanur (M1) and IR62829B x Ciherang (M2) and b. 17 doubled-haploid plants (DH2) potential as restorer lines derived from anther culture of F1 IR53942R x Ciherang (R1) and BR82735R x Sintanur (R2). All plant materials were exposed to BHP (biotype SU) and BLB straian IV and VIII. Bioassay with BHP biotype SU showed that for plants potential as maintainer lines their were 3 moderately susceptible lines and 12 susceptible lines, while for plants potential as restorer lines their were one resistance line (BioR-Ac-W-HD-15), one moderately resistance line (BioR-Ac-W-HD-16), 5 moderately susceptible lines and 10 susceptible lines. The result of bioassay with BLB strain IV and VIII in lines potential as maintainer were: a). For BLB strain IV we obtained one moderately resistance line (BioM-Ac-W-HD-4), while the rest (14 lines) were moderately susceptible, b). Forr BLB strain VIII we obtained 5 moderately resistance lines (BioM-Ac-W-HD-5,6,7,11 dan 12), while the other 10 lines were moderately susceptible. Furthermore, the result of bioassay with BLB strain IV and VIII in lines potential as restorer lines were: a). For BLB strain VI we obtained 2 moderately resistance lines (BioR-Ac-W-HD-15, and BioR-Ac-W-HD-16), while the other 15 lines were moderately susceptible, b). For BLB strain VIII we obtained 16 moderately resistance lines, i.e. BioR-Ac-W-HD-(1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, dan 17), while one line were moderately susceptible. All lines having resistance and moderately resistance to WBC biotype SU and/or HDB strain IV and VIII will be evaluated futher.},
    keywords = {doubled haploid, rice, bph, blb},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_152-158.pdf}
    }
  14. Husni, A., S. Hutami, M. Kosmiatin, and I. Mariska. 2004. Pembentukan Benih Somatik Dewasa Kedelai dan Aklimatisasi serta Uji terhadap Indikator Sifat Toleransi Kekeringan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 159-169.
    [BibTeX] [Abstract] [PDF: Pembentukan Benih Somatik Dewasa Kedelai dan Aklimatisasi serta Uji terhadap Indikator Sifat Toleransi Kekeringan ]
    Seleksi untuk toleransi/ketahanan terhadap kekeringan sangat kompleks karena adanya pengaruh interaksi antara genotipa dengan lingkungan yang menimbulkan perbedaan tanggap terhadap kekeringan. Kesulitan lain adalah kemampuan tanaman untuk tetap berproduksi tinggi pada kondisi kekeringan ditentukan oleh banyak faktor dan komponen, setiap faktor berbeda pada kondisi yang berbeda pula. Untuk mendapatkan tanaman kedelai yang toleran terhadap cekaman kekeringan te-lah dilakukan penelitian seleksi in vitro tanaman kedelai untuk toleransi terhadap kekeringan. Selek-si dilakukan dengan irradiasi sinar gamma dan komponen penyeleksi PEG. Konsentrasi PEG diten-tukan dengan penelitian penapisan in vitro pada genotipa-genotipa kedelai yang sudah diketahui tanggapnya terhadap cekaman kekeringan. Keberhasilan untuk mendapatkan kultivar atau varietas baru tanaman hasil kultur in vitro sangat ditentukan oleh keberhasilan memindahkan planlet yang dihasilkan ke lingkungan luar (aklimatisasi). Kondisi planlet yang berasal dari botol masih sangat rentan, terutama terhadap suhu dan kelembaban udara serta patogen dalam tanah. Keberhasilan tersebut sangat menentukan bisa tidaknya dilakukan pengamatan dan pengujian berikutnya ter-hadap keragaman yang diperoleh. Benih somatik muda tanaman kedelai varietas Sindoro dan Wilis yang toleran terhadap PEG dipindahkan ke media MS atau ½ MS tanpa zat pengatur tumbuh untuk perakaran sehingga terbentuk benih somatik dewasa. Benih somatik yang telah dewasa kemudian diaklimatisasi di rumah kaca dalam media campuran tanah dengan kompos (1 : 1) dan dipelihara sampai menghasilkan polong G1. Benih G1 diuji sifat toleransi kekeringannya dengan 2 komponen ketahanan, yaitu daya tembus akar dan kandungan prolin dalam daun. Uji daya tembus akar di-lakukan menggunakan campuran parafin dengan vaselin setebal 3 cm dengan perbandingan 60% : 40% yang setara dengan 12 bar dan analisis kandungan prolin pada daun yang mendapat cekaman kekeringan. Hasil pengujian yang dilakukan terhadap 15 somaklon hasil generasi pertama (G1) dari G0 yang tahan terhadap cekaman kekeringan akibat perlakuan PEG 20% diperoleh bahwa terdapat variasi kemampuan daya tembus akar terhadap lapisan uji di antara somaklon yang diuji. Hanya 7 somaklon yang mempunyai kemampuan menembus lapisan campuran parafin dan vaselin. Tiga somaklon mempunyai daya tembus akar yang lebih cepat dibandingkan varietas pembanding (Tanggamus dan Nanti), yaitu somaklon No. 2, No. 8, dan No. 12. Pengamatan terhadap komponen pertumbuhan, tidak memperlihatkan adanya korelasi antara daya tembus akar dengan panjang dan diameter akar. Hasil analisis kandungan prolin diperoleh 1 somaklon yang mempunyai kadar prolin jauh lebih tinggi dibandingkan dengan varietas pembanding, yaitu somaklon No. 4 dengan jumlah 1545 $\mu$mol/g berat basah daun. Sifat ketahanan terhadap cekaman kekeringan semua somaklon meningkat yang ditunjukkan oleh kandungan prolinnya lebih tinggi dari tanaman asal (Sindoro)
    @article{Husni04p159,
    title = {{Pembentukan Benih Somatik Dewasa Kedelai dan Aklimatisasi serta Uji terhadap Indikator Sifat Toleransi Kekeringan}},
    author = {A. Husni and S. Hutami and M. Kosmiatin and I. Mariska},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {159-169},
    year = {2004},
    abstract = {Seleksi untuk toleransi/ketahanan terhadap kekeringan sangat kompleks karena adanya pengaruh interaksi antara genotipa dengan lingkungan yang menimbulkan perbedaan tanggap terhadap kekeringan. Kesulitan lain adalah kemampuan tanaman untuk tetap berproduksi tinggi pada kondisi kekeringan ditentukan oleh banyak faktor dan komponen, setiap faktor berbeda pada kondisi yang berbeda pula. Untuk mendapatkan tanaman kedelai yang toleran terhadap cekaman kekeringan te-lah dilakukan penelitian seleksi in vitro tanaman kedelai untuk toleransi terhadap kekeringan. Selek-si dilakukan dengan irradiasi sinar gamma dan komponen penyeleksi PEG. Konsentrasi PEG diten-tukan dengan penelitian penapisan in vitro pada genotipa-genotipa kedelai yang sudah diketahui tanggapnya terhadap cekaman kekeringan. Keberhasilan untuk mendapatkan kultivar atau varietas baru tanaman hasil kultur in vitro sangat ditentukan oleh keberhasilan memindahkan planlet yang dihasilkan ke lingkungan luar (aklimatisasi). Kondisi planlet yang berasal dari botol masih sangat rentan, terutama terhadap suhu dan kelembaban udara serta patogen dalam tanah. Keberhasilan tersebut sangat menentukan bisa tidaknya dilakukan pengamatan dan pengujian berikutnya ter-hadap keragaman yang diperoleh. Benih somatik muda tanaman kedelai varietas Sindoro dan Wilis yang toleran terhadap PEG dipindahkan ke media MS atau ½ MS tanpa zat pengatur tumbuh untuk perakaran sehingga terbentuk benih somatik dewasa. Benih somatik yang telah dewasa kemudian diaklimatisasi di rumah kaca dalam media campuran tanah dengan kompos (1 : 1) dan dipelihara sampai menghasilkan polong G1. Benih G1 diuji sifat toleransi kekeringannya dengan 2 komponen ketahanan, yaitu daya tembus akar dan kandungan prolin dalam daun. Uji daya tembus akar di-lakukan menggunakan campuran parafin dengan vaselin setebal 3 cm dengan perbandingan 60% : 40% yang setara dengan 12 bar dan analisis kandungan prolin pada daun yang mendapat cekaman kekeringan. Hasil pengujian yang dilakukan terhadap 15 somaklon hasil generasi pertama (G1) dari G0 yang tahan terhadap cekaman kekeringan akibat perlakuan PEG 20% diperoleh bahwa terdapat variasi kemampuan daya tembus akar terhadap lapisan uji di antara somaklon yang diuji. Hanya 7 somaklon yang mempunyai kemampuan menembus lapisan campuran parafin dan vaselin. Tiga somaklon mempunyai daya tembus akar yang lebih cepat dibandingkan varietas pembanding (Tanggamus dan Nanti), yaitu somaklon No. 2, No. 8, dan No. 12. Pengamatan terhadap komponen pertumbuhan, tidak memperlihatkan adanya korelasi antara daya tembus akar dengan panjang dan diameter akar. Hasil analisis kandungan prolin diperoleh 1 somaklon yang mempunyai kadar prolin jauh lebih tinggi dibandingkan dengan varietas pembanding, yaitu somaklon No. 4 dengan jumlah 1545 $\mu$mol/g berat basah daun. Sifat ketahanan terhadap cekaman kekeringan semua somaklon meningkat yang ditunjukkan oleh kandungan prolinnya lebih tinggi dari tanaman asal (Sindoro)},
    keywords = {Kedelai, aklimatisasi, toleransi kekeringan},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_159-169.pdf}
    }
  15. Lestari, E. G., I. Mariska, D. Sukmadjaja, and D. Suardi. 2004. Seleksi In Vitro dan Identifikasi Tanaman Padi Varietas Gajahmungkur, Towuti, dan IR64 yang Tahan Kekeringan. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 170-179.
    [BibTeX] [Abstract] [PDF: Seleksi In Vitro dan Identifikasi Tanaman Padi Varietas Gajahmungkur, Towuti, dan IR64 yang Tahan Kekeringan ]
    Kebutuhan beras akhir-akhir ini terus meningkat, sementara itu produksi terus menurun yang disebabkan oleh kemarau panjang yang melanda beberapa daerah seperti di NTB, Jawa Tengah, Jawa Barat, dan lain-lain. Selain itu, beralihnya lahan subur menjadi pemukiman dan industri menyebabkan lahan potensial menjadi berkurang. Salah satu masalah yang dihadapi petani saat ini adalah masih terbatasnya bibit padi yang tahan kekeringan dan berproduksi tinggi sedangkan varie-tas padi gogo yang dikembangkan saat ini produksinya masih rendah selain itu tidak tahan terha-dap serangan penyakit blas. Untuk mengatasi hal ini perlu dilakukan penelitian untuk mendapatkan varietas padi yang berproduksi tinggi dan tahan cekaman kekeringan seperti pada varietas IR64. Untuk mengatasi kebutuhan bibit unggul tersebut, telah dilakukan penelitian induksi mutasi meng-gunakan sinar gamma dikombinasikan dengan kultur in vitro dan seleksi in vitro pada padi varietas Gajahmungkur, Towuti, dan IR64. Dari hasil penelitian berhasil diperoleh somaklon yang menunjuk-kan keragaman genetik yang tinggi dan tahan kekeringan. Untuk mengetahui mekanisme masing-masing genotipe terhadap cekaman kekeringan dan mendapatkan nomor yang tahan cekaman kekeringan telah dilakukan pengujian secara bertahap terhadap somaklon yang didapatkan, yaitu (1) penapisan dini menggunakan larutan PEG konsentrasi 20%, (2) uji daya tembus akar menggu-nakan lapisan lilin, (3) analisis kandungan prolin, (5) uji produksi pada kondisi cekaman kekeringan di rumah kaca. Hasil evaluasi tersebut telah diperoleh 23 somaklon Gajahmungkur, 9 somaklon Towuti, dan 13 somaklon dari varietas IR64 yang diduga tahan kekeringan berdasar uji PEG dan uji tembus akar serta kandungan prolinnya tinggi. Pemberian cekaman kekeringan pada somaklon yang diduga tahan kekeringan tersebut diperoleh 9 somaklon asal Towuti, 5 somaklon asal Gajah-mungkur, dan 8 somaklon asal IR64 yang unggul. Pada varietas Towuti dihasilkan tanaman dengan anakan lebih banyak dan tanaman lebih tinggi demikian pula produksi gabah. Pada varietas IR64 diperoleh somaklon yang dapat berbulir dalam kondisi cekaman kekeringan, sedangkan tanaman yang berasal dari benih tidak berbulir. Tanaman hasil seleksi tersebut menghasilkan anakan dan gabah isi lebih tinggi dibandingkan tanaman induknya.
    @article{Lestari04p170,
    title = {{Seleksi In Vitro dan Identifikasi Tanaman Padi Varietas Gajahmungkur, Towuti, dan IR64 yang Tahan Kekeringan}},
    author = {E. G. Lestari and I. Mariska and D. Sukmadjaja and D. Suardi},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {170-179},
    year = {2004},
    abstract = {Kebutuhan beras akhir-akhir ini terus meningkat, sementara itu produksi terus menurun yang disebabkan oleh kemarau panjang yang melanda beberapa daerah seperti di NTB, Jawa Tengah, Jawa Barat, dan lain-lain. Selain itu, beralihnya lahan subur menjadi pemukiman dan industri menyebabkan lahan potensial menjadi berkurang. Salah satu masalah yang dihadapi petani saat ini adalah masih terbatasnya bibit padi yang tahan kekeringan dan berproduksi tinggi sedangkan varie-tas padi gogo yang dikembangkan saat ini produksinya masih rendah selain itu tidak tahan terha-dap serangan penyakit blas. Untuk mengatasi hal ini perlu dilakukan penelitian untuk mendapatkan varietas padi yang berproduksi tinggi dan tahan cekaman kekeringan seperti pada varietas IR64. Untuk mengatasi kebutuhan bibit unggul tersebut, telah dilakukan penelitian induksi mutasi meng-gunakan sinar gamma dikombinasikan dengan kultur in vitro dan seleksi in vitro pada padi varietas Gajahmungkur, Towuti, dan IR64. Dari hasil penelitian berhasil diperoleh somaklon yang menunjuk-kan keragaman genetik yang tinggi dan tahan kekeringan. Untuk mengetahui mekanisme masing-masing genotipe terhadap cekaman kekeringan dan mendapatkan nomor yang tahan cekaman kekeringan telah dilakukan pengujian secara bertahap terhadap somaklon yang didapatkan, yaitu (1) penapisan dini menggunakan larutan PEG konsentrasi 20%, (2) uji daya tembus akar menggu-nakan lapisan lilin, (3) analisis kandungan prolin, (5) uji produksi pada kondisi cekaman kekeringan di rumah kaca. Hasil evaluasi tersebut telah diperoleh 23 somaklon Gajahmungkur, 9 somaklon Towuti, dan 13 somaklon dari varietas IR64 yang diduga tahan kekeringan berdasar uji PEG dan uji tembus akar serta kandungan prolinnya tinggi. Pemberian cekaman kekeringan pada somaklon yang diduga tahan kekeringan tersebut diperoleh 9 somaklon asal Towuti, 5 somaklon asal Gajah-mungkur, dan 8 somaklon asal IR64 yang unggul. Pada varietas Towuti dihasilkan tanaman dengan anakan lebih banyak dan tanaman lebih tinggi demikian pula produksi gabah. Pada varietas IR64 diperoleh somaklon yang dapat berbulir dalam kondisi cekaman kekeringan, sedangkan tanaman yang berasal dari benih tidak berbulir. Tanaman hasil seleksi tersebut menghasilkan anakan dan gabah isi lebih tinggi dibandingkan tanaman induknya.},
    keywords = {Radiasi seleksi in vitro, PEG, tahan kekeringan, padi},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_170-179.pdf}
    }
  16. Mariska, I., Y. Supriati, and S. Hutami. 2004. Mikropropagasi Sukun (Artocarpus communis Forst), Tanaman Sumber Karbohidrat Alternatif. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 180-188.
    [BibTeX] [Abstract] [PDF: Mikropropagasi Sukun (Artocarpus communis Forst), Tanaman Sumber Karbohidrat Alternatif ]
    Bread fruit (Artocarpus communis Forst) is one of tropical fruit, which has a high contain of carbohydrate. In certain area, it becomes an alternative staple food when the main staple foods are scarce. The amount of carbohydrate in breadfruit is almost the same with the one in sweet potato, but it is higher than in potato. The main constraint of the development of breadfruit is the limited of seedling availability. Tissue culture technique has been known for its excellent result for plant propagation, because this technique has ability in producing seedling in a large quantity, in uniform growth rate and in a relative short time. The experiment was conducted at Cell Tissue Culture Division, Indonesian Center Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRAD) from February 2003 until December 2004. There were some steps experiments with series of combination medium as treatments. The first steps was shoot multiplication at Sk-2 medium with WPM + BA (0, 0.5, 1.0; 1.5, and 2.0 mg/l) + thidiazuron (0, 0.4 mg/l); The second step was elongation shoot at Sk-3 with WPM + kinetin (1, 2, and 3 mg/l) + GA3 (0 and 5 mg/l), and the third was root initiation and proliferation, by comparing WPM + IBA (0, 2, 4, and 6 mg/l) + charcoal (0, 0.5%) and WPM (1, 0.5) + BA (0, 1.5, and 5 mg.l) or NAA (1, 2, and 3 mg/l). The result showed that second subculture (Sk-2) in WPM + BA 1.5-2 mg/l + thidiazuron 0.4 mg/l with 1-2 cm shoot increase shoot multiplication 4.87-5. High frequency of subculture until 3 times gave the highest shoot multiplication 15.5. WPM + kinetin 1 mg/l + GA3 5 mg/l was the best medium for shoot elongation. The highest root percentage was 60% with number of root was 6,5 from WPM + IBA 3 mg/l. Acclimatization was done in green house with the rate of success of 70%.
    @article{Mariska04p180,
    title = {{Mikropropagasi Sukun (Artocarpus communis Forst), Tanaman Sumber Karbohidrat Alternatif}},
    author = {I. Mariska and Y. Supriati and S. Hutami},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {180-188},
    year = {2004},
    abstract = {Bread fruit (Artocarpus communis Forst) is one of tropical fruit, which has a high contain of carbohydrate. In certain area, it becomes an alternative staple food when the main staple foods are scarce. The amount of carbohydrate in breadfruit is almost the same with the one in sweet potato, but it is higher than in potato. The main constraint of the development of breadfruit is the limited of seedling availability. Tissue culture technique has been known for its excellent result for plant propagation, because this technique has ability in producing seedling in a large quantity, in uniform growth rate and in a relative short time. The experiment was conducted at Cell Tissue Culture Division, Indonesian Center Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRAD) from February 2003 until December 2004. There were some steps experiments with series of combination medium as treatments. The first steps was shoot multiplication at Sk-2 medium with WPM + BA (0, 0.5, 1.0; 1.5, and 2.0 mg/l) + thidiazuron (0, 0.4 mg/l); The second step was elongation shoot at Sk-3 with WPM + kinetin (1, 2, and 3 mg/l) + GA3 (0 and 5 mg/l), and the third was root initiation and proliferation, by comparing WPM + IBA (0, 2, 4, and 6 mg/l) + charcoal (0, 0.5%) and WPM (1, 0.5) + BA (0, 1.5, and 5 mg.l) or NAA (1, 2, and 3 mg/l). The result showed that second subculture (Sk-2) in WPM + BA 1.5-2 mg/l + thidiazuron 0.4 mg/l with 1-2 cm shoot increase shoot multiplication 4.87-5. High frequency of subculture until 3 times gave the highest shoot multiplication 15.5. WPM + kinetin 1 mg/l + GA3 5 mg/l was the best medium for shoot elongation. The highest root percentage was 60% with number of root was 6,5 from WPM + IBA 3 mg/l. Acclimatization was done in green house with the rate of success of 70%.},
    keywords = {bread fruit, artocarpus communis forst, shoot multiplication, root initiation and proliferation, acclimatization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_180-188.pdf}
    }
  17. Supriati, Y., I. Mariska, A. Husni, and S. Hutami. 2004. Inisiasi dan Perkembangan Perakaran serta Aklimatisasi Belimbing Dewi (Averrhoa carambola L.). Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 189-194.
    [BibTeX] [Abstract] [PDF: Inisiasi dan Perkembangan Perakaran serta Aklimatisasi Belimbing Dewi (Averrhoa carambola L.) ]
    Star fruit (Averrhoa carambola) as a source of vitamin C is originally comes from Malaysia. In Chinnese society starfruit is well known as medicinal plant which was useful for decreasing blood pressure. In daily menu, this fruit usually serve as fresh consumtion, but now is also used as raw material industry of jam, pickles, and juice. Main constraints on fruit star development are the limitation of seedlings availability. In previous research, formula media for initiation and shoot multiplication of star fruit was obtained, and this research will be focused on searching formula media for root initiation and proliferation, besides on the best growth media for acclimatization. For root initiation, shoots were planted at WPM basal media in combination with three rates of IBA and IAA (0, 1, dan 3 mg/l) and at MS basal media in combination with four rates of IBA and NAA (0, 1, 2, dan 3 mg/l). For efficiency, macro element of WPM was decreased by 50% in combination five rates of IBA (0, 1, 3, 5, dan 7 mg/l). Acclimatization test was done by using three kind of media such as soil, organic matter, and compost which was applied in single or in combination. Parameter observed were rooting percentage of planlet, root number and length, root performance, and success percentage of growth. Result showed that the use of basal media WPM applied with IBA and IAA at the rate of 1-3 mg/l could not stimulate root initiation. Meanawhile root was initiate when basal media MS was applied with 2 mg/l of zeatin and 3 mg/l of IBA although the root number was still limited. A half formula of WPM basal media applied with 3 mg/l of IBA was the best media for root initiation and proliferation. Soil and compost in combination of 1 : 2 was the best growth media for acclimatization of starfruit in the greenhouse. Acclimatization of starfuit planlet could be done directly without rooting stage by using IBA treatment at 100 ppm during one hour.
    @article{Supriati04p189,
    title = {{Inisiasi dan Perkembangan Perakaran serta Aklimatisasi Belimbing Dewi (Averrhoa carambola L.)}},
    author = {Y. Supriati and I. Mariska and A. Husni and S. Hutami},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {189-194},
    year = {2004},
    abstract = {Star fruit (Averrhoa carambola) as a source of vitamin C is originally comes from Malaysia. In Chinnese society starfruit is well known as medicinal plant which was useful for decreasing blood pressure. In daily menu, this fruit usually serve as fresh consumtion, but now is also used as raw material industry of jam, pickles, and juice. Main constraints on fruit star development are the limitation of seedlings availability. In previous research, formula media for initiation and shoot multiplication of star fruit was obtained, and this research will be focused on searching formula media for root initiation and proliferation, besides on the best growth media for acclimatization. For root initiation, shoots were planted at WPM basal media in combination with three rates of IBA and IAA (0, 1, dan 3 mg/l) and at MS basal media in combination with four rates of IBA and NAA (0, 1, 2, dan 3 mg/l). For efficiency, macro element of WPM was decreased by 50% in combination five rates of IBA (0, 1, 3, 5, dan 7 mg/l). Acclimatization test was done by using three kind of media such as soil, organic matter, and compost which was applied in single or in combination. Parameter observed were rooting percentage of planlet, root number and length, root performance, and success percentage of growth. Result showed that the use of basal media WPM applied with IBA and IAA at the rate of 1-3 mg/l could not stimulate root initiation. Meanawhile root was initiate when basal media MS was applied with 2 mg/l of zeatin and 3 mg/l of IBA although the root number was still limited. A half formula of WPM basal media applied with 3 mg/l of IBA was the best media for root initiation and proliferation. Soil and compost in combination of 1 : 2 was the best growth media for acclimatization of starfruit in the greenhouse. Acclimatization of starfuit planlet could be done directly without rooting stage by using IBA treatment at 100 ppm during one hour.},
    keywords = {in vitro culture, averhoa carambola l, root initiation, acclimatization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_189-194.pdf}
    }
  18. Pratiwi, E., A. Akhdiya, I. M. Samudra, and B. Soegiarto. 2004. Isolasi Gen Penyandi Toksin Insektisidal dari Bakteri Simbion Nematoda Patogen Serangga. Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 195-200.
    [BibTeX] [Abstract] [PDF: Isolasi Gen Penyandi Toksin Insektisidal dari Bakteri Simbion Nematoda Patogen Serangga ]
    Frequently and high dose use of synthetic pesticide is one of the factors causing low quality of land for agricultural production. One of the efforts to reduce dependence on chemical pesticides is application of biological control or biopesticide. Naturally many bacteria produce insects toxins that can be used as biopestices. Bacteria that symbiotically associate with specific nematodes (Photorhabdus sp. or Xenorhabdus sp.) with its many insect toxins now provides some options, because their broad-spectrum toxins can be used to kill insects ranging from Lepidoptera, Coleoptera to Dictyoptera. The insecticidal toxin genes from symbiotic bacteria entomopathogenic nematodes would be used as material for sprayable agent or biocontrol agent and construction of insect resistant transgenic plant. We have purified the insecticidal toxins from three isolates of Photorhabdus sp. employing AKTA Purifier System. All of the toxins have the similar chromatogram patterns; they were eluted at volume 38-40 ml. We have designed two PCR primers, i.e. primer reverse (5′-ACGCTCATCACCCCAAAA-3′) and primer forward (5′-TGTCAATGCCCGCTACAA-3′) to detect mcf gene of Photorhabdus sp. Amplification of DNA genomic of Photorhabdus sp. using these primers generated a 789 bp-amplicon.
    @article{Pratiwi04p195,
    title = {{Isolasi Gen Penyandi Toksin Insektisidal dari Bakteri Simbion Nematoda Patogen Serangga}},
    author = {E. Pratiwi and A. Akhdiya and I. M. Samudra and B. Soegiarto},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {195-200},
    year = {2004},
    abstract = {Frequently and high dose use of synthetic pesticide is one of the factors causing low quality of land for agricultural production. One of the efforts to reduce dependence on chemical pesticides is application of biological control or biopesticide. Naturally many bacteria produce insects toxins that can be used as biopestices. Bacteria that symbiotically associate with specific nematodes (Photorhabdus sp. or Xenorhabdus sp.) with its many insect toxins now provides some options, because their broad-spectrum toxins can be used to kill insects ranging from Lepidoptera, Coleoptera to Dictyoptera. The insecticidal toxin genes from symbiotic bacteria entomopathogenic nematodes would be used as material for sprayable agent or biocontrol agent and construction of insect resistant transgenic plant. We have purified the insecticidal toxins from three isolates of Photorhabdus sp. employing AKTA Purifier System. All of the toxins have the similar chromatogram patterns; they were eluted at volume 38-40 ml. We have designed two PCR primers, i.e. primer reverse (5'-ACGCTCATCACCCCAAAA-3') and primer forward (5'-TGTCAATGCCCGCTACAA-3') to detect mcf gene of Photorhabdus sp. Amplification of DNA genomic of Photorhabdus sp. using these primers generated a 789 bp-amplicon.},
    keywords = {photorhabdus sp, entomopathogenic bacteria, insecticidal toxins, pcr primer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_195-200.pdf}
    }
  19. Manzila, I., J. Harjosudarmo, M. Machmud, and Y. Suryadi. 2004. Produksi Antibodi Monoklonal untuk Deteksi Dini Rice Tungro Virus (RTV). Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 201-218.
    [BibTeX] [Abstract] [PDF: Produksi Antibodi Monoklonal untuk Deteksi Dini Rice Tungro Virus (RTV) ]
    Rice tungro virus (RTV) is the important disease that reduce 10%-outbreak. Alternatif methode to control is by detection used monoclonal antibody. Mice balb/c was immunized by injecting with RTV antigen periodic a week. Limphoccytes from immunized mice were harvested and used for cell fusion with SP2 myelomas cell to produce hybridomas. Results of the trial showed that 22 hybridomas colonies producing McAb RTV were obtained from cell fusion. Two hybridoma colonies potential as sources for McAb RTV productions were obtained,ie., RTV-2B2 and RTV-3D7. These hybridomas were stored under a cryogenic condition in a liqiud nitrogen for further works.
    @article{Manzila04p201,
    title = {{Produksi Antibodi Monoklonal untuk Deteksi Dini Rice Tungro Virus (RTV)}},
    author = {I. Manzila and J. Harjosudarmo and M. Machmud and Y. Suryadi},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {201-218},
    year = {2004},
    abstract = {Rice tungro virus (RTV) is the important disease that reduce 10%-outbreak. Alternatif methode to control is by detection used monoclonal antibody. Mice balb/c was immunized by injecting with RTV antigen periodic a week. Limphoccytes from immunized mice were harvested and used for cell fusion with SP2 myelomas cell to produce hybridomas. Results of the trial showed that 22 hybridomas colonies producing McAb RTV were obtained from cell fusion. Two hybridoma colonies potential as sources for McAb RTV productions were obtained,ie., RTV-2B2 and RTV-3D7. These hybridomas were stored under a cryogenic condition in a liqiud nitrogen for further works.},
    keywords = {monoclonal antibody, detection, rice tungro virus, rtv},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_201-218.pdf}
    }
  20. Machmud, M., J. Harjosudarmo, I. Manzila, and Y. d. Suryadi. 2004. Pengembangan Teknik Produksi dan Aplikasi Antibodi Monoklonal Ralstonia Solanacearum (RS). Kumpulan makalah seminar hasil penelitian bb-biogen tahun 2004 219-228.
    [BibTeX] [Abstract] [PDF: Pengembangan Teknik Produksi dan Aplikasi Antibodi Monoklonal Ralstonia Solanacearum (RS) ]
    Technique for production of monoclonal antibody for the detection of Ralstonia slanacearum. The success of an integrated control of a plant disease, such as bacterial wilt (Ralstonia solanacearum, RS) is very much dependent on accurate detection of the pathogen and epidemiology of the disease. Serological techniques, such as ELISA is a promising technique to obtain the information, since they are relatively simple, accurate, and applicable in the field. Sensitivity of the technique depends on specificity of the antibody. Monoclonal antibody generally has a specific reaction to the target pathogen. A research activity was conducted to produce and screen hybridoma cells capable of producing monoclonal antibodies specific to RS. Production of hybridomas was done through fusions of SP2 myeloma cells with lymphocytes of mice hybrid Balb c that have been immunized with an RS antigen. Hybridoma cells were produced from the fusions. Eight hybridoma clones potential of producing MAb RS were selected and stored cryogenically in the laboratory. Specificity of the McAb produced by the hybridomas is being done. The selected hybridomas may be stored for an unlimited time and used as sources for production of MAb RS whenever needed.
    @article{Machmud04p219,
    title = {{Pengembangan Teknik Produksi dan Aplikasi Antibodi Monoklonal Ralstonia Solanacearum (RS)}},
    author = {M. Machmud and J. Harjosudarmo and I. Manzila and d. Y. Suryadi},
    journal = {Kumpulan Makalah Seminar Hasil Penelitian BB-Biogen Tahun 2004},
    pages = {219-228},
    year = {2004},
    abstract = {Technique for production of monoclonal antibody for the detection of Ralstonia slanacearum. The success of an integrated control of a plant disease, such as bacterial wilt (Ralstonia solanacearum, RS) is very much dependent on accurate detection of the pathogen and epidemiology of the disease. Serological techniques, such as ELISA is a promising technique to obtain the information, since they are relatively simple, accurate, and applicable in the field. Sensitivity of the technique depends on specificity of the antibody. Monoclonal antibody generally has a specific reaction to the target pathogen. A research activity was conducted to produce and screen hybridoma cells capable of producing monoclonal antibodies specific to RS. Production of hybridomas was done through fusions of SP2 myeloma cells with lymphocytes of mice hybrid Balb c that have been immunized with an RS antigen. Hybridoma cells were produced from the fusions. Eight hybridoma clones potential of producing MAb RS were selected and stored cryogenically in the laboratory. Specificity of the McAb produced by the hybridomas is being done. The selected hybridomas may be stored for an unlimited time and used as sources for production of MAb RS whenever needed.},
    keywords = {ralstonia solanacearum, monoclonal antibody},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2004_219-228.pdf}
    }

2003

  1. Hadiatmi, Ida H. Somantri, Tiur S. Silitonga, Sri G. Budiarti, Sri A. Rais, Nani Zuraida, Minantyorini, Lukman Hakim, Tintin Suhartini, Nurwita Dewi, and Mamik Setyowati. 2003. Rejuvenasi, Karakterisasi Morfologi, dan Mutu Gizi Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 1-32.
    [BibTeX] [Abstract] [PDF: Rejuvenasi, Karakterisasi Morfologi, dan Mutu Gizi Plasma Nutfah Tanaman Pangan ]
    The objectives of the experiment were to conserve food crops germplasm collection and to characterize the morpho -agronomical and nutritional characters. The experiments were conducted in the dry and rainy season of 2002. A total of 1513 accessions of cereals, 1640 accessions of legumes, and 2562 accessions of root crops were rejuvenated at several research instalations such as Cikeumeuh, Muara, Citayam, Pacet, Pusakanegara, and Kuningan. The results showed that 24 accessions of rice have long panicle (>30 cm) and heavy grain weight (>30 cm); 17 accessions of corn have big grain sizes by 300-grain weight (>80 g); 2 accessions of wheat yielded >2.5 kg/plot; 6 accessions of sorghum have heavy grain weight per panicle (>80 g) and big number of grain per panicle (>2000 grain); 9 accessions of soybean have big grain sizes as well by 100 grain weight (10.5 g); 33 accessions of ground nut have big number of pod/plant (>20 pods); 13 accessions of mungbean have high grain yield per plant (12.7-22.3 g) and also high in 1000 grain weight (57.7-67.3 g); seven accessions of cassava yielded >3,0 kg/plant; 5 accessions of sweetpotato were resistance to Cylas formicarius; 3 accessions of arrowroot yielded about 1.0-1.2 kg per hill; 4 accessions of edible canna yielded 2.5 -2.84 kg per plant; 4 accessions of big yam (Dioscorea alata) yielded 4.7-13.0 kg/plant and 5 accessions of little yam (D. esculenta) were 1.2-2.25 kg. The results of nutritional characterization show that 7 accessions of glutinous rice have low amylose content (<10%) and 18 accessions of non glutinous rice have amylose content (10-23%); 5 glutinous corn have amylose content <20%; 7 accessions of soybean have high protein content (39%); 5 accessions of ground nut have protein content varied from 27.3-28.0% and another 2 accessions with fat content varied from 35-38%. There were 7 accessions of sweetpotato and 2 accessions of big yam have high starch content (>50%). A total of 12 accessions of cassava have low HCN content (<22 ppm) and 14 accessions of sorghum have low tannin (<0.25%).
    @article{Hadiatmi03p1,
    title = {{Rejuvenasi, Karakterisasi Morfologi, dan Mutu Gizi Plasma Nutfah Tanaman Pangan}},
    author = {Hadiatmi and Ida H. Somantri and Tiur S. Silitonga and Sri G. Budiarti and Sri A. Rais and Nani Zuraida and
    Minantyorini and Lukman Hakim and Tintin Suhartini and Nurwita Dewi and Mamik Setyowati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {1-32},
    year = {2003},
    abstract = {The objectives of the experiment were to conserve food crops germplasm collection and to characterize the morpho -agronomical and nutritional characters. The experiments were conducted in the dry and rainy season of 2002. A total of 1513 accessions of cereals, 1640 accessions of legumes, and 2562 accessions of root crops were rejuvenated at several research instalations such as Cikeumeuh, Muara, Citayam, Pacet, Pusakanegara, and Kuningan. The results showed that 24 accessions of rice have long panicle (>30 cm) and heavy grain weight (>30 cm); 17 accessions of corn have big grain sizes by 300-grain weight (>80 g); 2 accessions of wheat yielded >2.5 kg/plot; 6 accessions of sorghum have heavy grain weight per panicle (>80 g) and big number of grain per panicle (>2000 grain); 9 accessions of soybean have big grain sizes as well by 100 grain weight (10.5 g); 33 accessions of ground nut have big number of pod/plant (>20 pods); 13 accessions of mungbean have high grain yield per plant (12.7-22.3 g) and also high in 1000 grain weight (57.7-67.3 g); seven accessions of cassava yielded >3,0 kg/plant; 5 accessions of sweetpotato were resistance to Cylas formicarius; 3 accessions of arrowroot yielded about 1.0-1.2 kg per hill; 4 accessions of edible canna yielded 2.5 -2.84 kg per plant; 4 accessions of big yam (Dioscorea alata) yielded 4.7-13.0 kg/plant and 5 accessions of little yam (D. esculenta) were 1.2-2.25 kg. The results of nutritional characterization show that 7 accessions of glutinous rice have low amylose content (<10%) and 18 accessions of non glutinous rice have amylose content (10-23%); 5 glutinous corn have amylose content <20%; 7 accessions of soybean have high protein content (39%); 5 accessions of ground nut have protein content varied from 27.3-28.0% and another 2 accessions with fat content varied from 35-38%. There were 7 accessions of sweetpotato and 2 accessions of big yam have high starch content (>50%). A total of 12 accessions of cassava have low HCN content (<22 ppm) and 14 accessions of sorghum have low tannin (<0.25%).},
    keywords = {Food crops germplasm, rejuvenation, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_1-32_hadiatmi.pdf}
    }
  2. Silitonga, T. S., S. G. Budiarti, S. A. Rais, I. H. Somantri, and M. Machmud. 2003. Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Jagung terhadap Bulai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 33-41.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Jagung terhadap Bulai ]
    The experiment were conducted in dry and wet season of 2002 in the greenhouse of Indonesian Biotechnology and Genetic Resources Research Institute, Cikeumeuh Experimental Farm, and Cimenteng, Sukabumi. The results showed that some rice germplasm have resistant reaction to bacterial leaf blight (BLB) group IV and VIII: Mendalet (Acc. 5657), Tholo (Acc. 5720), Angkong (Acc. 7237), Kayok (Acc. KBP57), and Ringka (Acc. KBP118); and 10 varieties have resistant to blast disease: Loneng, Pembagi, Padi Jangkong, Kayok, Pandan, Bungung Inih, Pulut Timun, Pulut Timau, Pulut Alui, and Pulut Namang. Evaluation of corn germplasm to downy mildew showed 20 accessions have very resistant with the attack percentage ranged between 0-5%. All rice and corn germplasm which have resistant to disease could be used as gene resources in breeding program varieties improvement to diseases.
    @article{Silitonga03p33,
    title = {{Evaluasi Ketahanan Plasma Nutfah Padi terhadap Penyakit Hawar Daun Bakteri dan Blas, dan Jagung terhadap Bulai}},
    author = {T. S. Silitonga and S. G. Budiarti and S. A. Rais and I. H. Somantri and M. Machmud},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {33-41},
    year = {2003},
    abstract = {The experiment were conducted in dry and wet season of 2002 in the greenhouse of Indonesian Biotechnology and Genetic Resources Research Institute, Cikeumeuh Experimental Farm, and Cimenteng, Sukabumi. The results showed that some rice germplasm have resistant reaction to bacterial leaf blight (BLB) group IV and VIII: Mendalet (Acc. 5657), Tholo (Acc. 5720), Angkong (Acc. 7237), Kayok (Acc. KBP57), and Ringka (Acc. KBP118); and 10 varieties have resistant to blast disease: Loneng, Pembagi, Padi Jangkong, Kayok, Pandan, Bungung Inih, Pulut Timun, Pulut Timau, Pulut Alui, and Pulut Namang. Evaluation of corn germplasm to downy mildew showed 20 accessions have very resistant with the attack percentage ranged between 0-5%. All rice and corn germplasm which have resistant to disease could be used as gene resources in breeding program varieties improvement to diseases.},
    keywords = {germplasm, evaluation, disease},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_33-41_sudiaty.pdf}
    }
  3. Zuraida, N., T. S. S. ilitonga, Suyono, Minantyorini, and D. Koswanudin. 2003. Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 42-48.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar) ]
    Evaluation were done in IABGRRI green house on DS of 2002. Three hundreed accessions of rice germplasm were infested by 2-3 instar larvae of IR42 and IR64 populations with 3-4 bugs/plant, respectively at seven days old-plant. Evaluation on its resistance were scored after IR42 and IR64 as a control showed 90% died. The total of fifty accessions of sweetpotato germplasm were infested by 3 pairs of Cylas formicarius , respectively on tuber root. Evaluation were done after 30 days of infestation. The result showed that none of rice germplasm had resistance to rice brownplanthopper of IR42 and IR64 population, 5.7% of evaluated accessions had moderate resistant on IR42 population and 5% to IR64 population. One of 50 accessions was resistant to sweetpotato weevil and nine accessions was moderate resistant.
    @article{Zuraida03p42,
    title = {{Evaluasi Ketahanan Plasma Nutfah Tanaman terhadap Hama (Wereng Coklat pada Padi dan Hama Lanas pada Ubi Jalar)}},
    author = {N. Zuraida and T. S. S. ilitonga and Suyono and Minantyorini and D. Koswanudin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {42-48},
    year = {2003},
    abstract = {Evaluation were done in IABGRRI green house on DS of 2002. Three hundreed accessions of rice germplasm were infested by 2-3 instar larvae of IR42 and IR64 populations with 3-4 bugs/plant, respectively at seven days old-plant. Evaluation on its resistance were scored after IR42 and IR64 as a control showed 90% died. The total of fifty accessions of sweetpotato germplasm were infested by 3 pairs of Cylas formicarius , respectively on tuber root. Evaluation were done after 30 days of infestation. The result showed that none of rice germplasm had resistance to rice brownplanthopper of IR42 and IR64 population, 5.7% of evaluated accessions had moderate resistant on IR42 population and 5% to IR64 population. One of 50 accessions was resistant to sweetpotato weevil and nine accessions was moderate resistant.},
    keywords = {rice, sweetpotato, germplasm, brown planthopper, weevil},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_42-48_nanizuraida.pdf}
    }
  4. Budiarti, S. G., T. Suhartini, T. S. Silitonga, N. Dewi, and Hadiatmi. 2003. Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Lahan Masam, Keracunan Al, dan Fe). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 49-57.
    [BibTeX] [Abstract] [PDF: Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Lahan Masam, Keracunan Al, dan Fe) ]
    The aim of these activities were to get rice genotipes that tolerant to Al and Fe toxicity and to get maize and soybean genotipes tolerant to Al toxicity. The activities that included evaluation of rice to Al and Fe toxicity, and maize and soybean to Al toxicity have been done at Research Instalation Experiment, Tamanbogo, Lampung in WS 2002. The total accessions of rice tested to Al toxicity were 200 accessions and for maize and soybean were 100 accessions. The experiment used Randomized Complete Block Design with 3 replications, except for soybean only 2 replications. The results showed that testing for rice germplasm to Al toxicity found 23 accs. tolerant with score 1-3, two of them have score 1 were Tayan (Reg. no. 21228) and Segeret (Reg. no. 21230). There were ten accessions of maize germplasm tolerant to Al toxicity with score 2-3. Three accessions were Kelinci (Reg. no. 2006), Leha-leha (Reg. no. 2026), and Lokal Anyar (Reg. no. 3176) have score 2.2- 2.3. Thirteen accessions of soybean germplasm tolerant to Al toxicity with score 1-2, some of them were 317/882, B4194, C73-01-113A-C- Pop. There were 16 accessions of rice tolerant to Fe toxicity with score 1-3 on 8 WAT Randah Padang K. (Reg. no. 4323), Pulu Denni b (Reg. no. 4372), Getik (Reg. no. 5643), Mendalet (Reg. no. 5657), Menta (Reg. no. 5758), Rojolele (Reg. no. 5813), Ritgen (Reg. no. 6550), Jedah (Reg. no. 6601, Ketan (Reg. no. 6856), Sampang K (Reg. no. 6857), Komas A (Reg. no. 6877A), Komas B (Reg. no. 6877b), Sipulo (Reg. no. 8025), Genjah Mada (Reg. no. 5856), Pudak Kuning (Reg. no. 6204), dan Cere Bandung (Reg. no. 6858).
    @article{Budiarti03p49,
    title = {{Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Lahan Masam, Keracunan Al, dan Fe)}},
    author = {S. G. Budiarti and T. Suhartini and T. S. Silitonga and N. Dewi and Hadiatmi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {49-57},
    year = {2003},
    abstract = { The aim of these activities were to get rice genotipes that tolerant to Al and Fe toxicity and to get maize and soybean genotipes tolerant to Al toxicity. The activities that included evaluation of rice to Al and Fe toxicity, and maize and soybean to Al toxicity have been done at Research Instalation Experiment, Tamanbogo, Lampung in WS 2002. The total accessions of rice tested to Al toxicity were 200 accessions and for maize and soybean were 100 accessions. The experiment used Randomized Complete Block Design with 3 replications, except for soybean only 2 replications. The results showed that testing for rice germplasm to Al toxicity found 23 accs. tolerant with score 1-3, two of them have score 1 were Tayan (Reg. no. 21228) and Segeret (Reg. no. 21230). There were ten accessions of maize germplasm tolerant to Al toxicity with score 2-3. Three accessions were Kelinci (Reg. no. 2006), Leha-leha (Reg. no. 2026), and Lokal Anyar (Reg. no. 3176) have score 2.2- 2.3. Thirteen accessions of soybean germplasm tolerant to Al toxicity with score 1-2, some of them were 317/882, B4194, C73-01-113A-C- Pop. There were 16 accessions of rice tolerant to Fe toxicity with score 1-3 on 8 WAT Randah Padang K. (Reg. no. 4323), Pulu Denni b (Reg. no. 4372), Getik (Reg. no. 5643), Mendalet (Reg. no. 5657), Menta (Reg. no. 5758), Rojolele (Reg. no. 5813), Ritgen (Reg. no. 6550), Jedah (Reg. no. 6601, Ketan (Reg. no. 6856), Sampang K (Reg. no. 6857), Komas A (Reg. no. 6877A), Komas B (Reg. no. 6877b), Sipulo (Reg. no. 8025), Genjah Mada (Reg. no. 5856), Pudak Kuning (Reg. no. 6204), dan Cere Bandung (Reg. no. 6858).},
    keywords = {Rice, maize, soybean, evaluation, Al and Fe toxicity},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_49-57_srigajatri.pdf}
    }
  5. Minantyorini, Hakim Kurniawan, Mamik Setyowati, Tiur S. Silitonga, Hadiatmi, Sri G. Budiarti, Sri A. Rais, Nani Zuraida, Lukman Hakim, Sutoro, Asadi, and Tintin Suhartini. 2003. Pengembangan Database Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 58-65.
    [BibTeX] [Abstract] [PDF: Pengembangan Database Plasma Nutfah Tanaman Pangan ]
    Food crops germplasm management consisted of exploration, registration of accessions, preservation, characterization, evaluation, and utilization of its accession(s) for breeding program. Each of these activities produce a lot of valuable data and information that have to be documented systematically. Consequently, a database system should be established to ensure the availabil-ity of data and information that can be easily, rapidly, and accurately accessed. Since 1998, the Microsoft Access 2000 – based of database system has been developed for managing food crops germplasm data. Until January 2003, a total of 7983 data records w ere managed, which was consisted of 3 563 records of rice on 41 descriptors, 705 records of corn on 29 descriptors, 174 records of sorghum on 22 descriptors, 771 records of soybean on 28 descriptors, 270 records of groundnut on 19 descriptors, 1024 records of mungbean on 20 descriptors, 434 records of cassava on 23 descriptors, 912 records of sweetpotato on 35 descriptors, and 130 records of cowpea on 22 descriptors. All of recorded data were compiled as a food crop catalogue that will be updated annually. This catalogue book is expected to become a useful information for any users. In addition, a database system on compact disk and internet is beeing developed to enhance data accessibility on the network users.
    @article{Minantyorini03p58,
    title = {{Pengembangan Database Plasma Nutfah Tanaman Pangan}},
    author = {Minantyorini and Hakim Kurniawan and Mamik Setyowati and Tiur S. Silitonga and Hadiatmi and Sri G. Budiarti and Sri A. Rais and Nani Zuraida and Lukman Hakim and Sutoro and Asadi, and Tintin Suhartini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {58-65},
    year = {2003},
    abstract = {Food crops germplasm management consisted of exploration, registration of accessions, preservation, characterization, evaluation, and utilization of its accession(s) for breeding program. Each of these activities produce a lot of valuable data and information that have to be documented systematically. Consequently, a database system should be established to ensure the availabil-ity of data and information that can be easily, rapidly, and accurately accessed. Since 1998, the Microsoft Access 2000 - based of database system has been developed for managing food crops germplasm data. Until January 2003, a total of 7983 data records w ere managed, which was consisted of 3 563 records of rice on 41 descriptors, 705 records of corn on 29 descriptors, 174 records of sorghum on 22 descriptors, 771 records of soybean on 28 descriptors, 270 records of groundnut on 19 descriptors, 1024 records of mungbean on 20 descriptors, 434 records of cassava on 23 descriptors, 912 records of sweetpotato on 35 descriptors, and 130 records of cowpea on 22 descriptors. All of recorded data were compiled as a food crop catalogue that will be updated annually. This catalogue book is expected to become a useful information for any users. In addition, a database system on compact disk and internet is beeing developed to enhance data accessibility on the network users.},
    keywords = {database, germplasm, food crops},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_58-65_minantyorini.pdf}
    }
  6. Somantri, I. H., T. J. Santoso, Minantyorini, A. D. Ambarwati, A. Sisharmini, and A. Apriana. 2003. Karakterisasi Molekuler Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 66-74.
    [BibTeX] [Abstract] [PDF: Karakterisasi Molekuler Plasma Nutfah Tanaman Pangan ]
    The research have been done in the year 2002, for rice germplasm from East Kalimantan and sweetpotato from Irian Jaya. Molecular characterization was done to know their genetics diversity and similarity. This research was the first activity to characterize DNA level of germplasm collection in Indonesian Agri-cultural Biotechnology and Genetics Resources Research Institute (IABGRRI). DNA isolation was done as a target, whereas PCR analysis was only done in sweetpotato. The results of PR analysis were random primer’s of A01, A04, and B01 could be amplified the DNA on 97 accessions of sweetpotato germplasm with DNA band varied between 1-9.
    @article{Somantri03p66,
    title = {{Karakterisasi Molekuler Plasma Nutfah Tanaman Pangan}},
    author = {I. H. Somantri and T. J. Santoso and Minantyorini and A. D. Ambarwati and A. Sisharmini and A. Apriana},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {66-74},
    year = {2003},
    abstract = {The research have been done in the year 2002, for rice germplasm from East Kalimantan and sweetpotato from Irian Jaya. Molecular characterization was done to know their genetics diversity and similarity. This research was the first activity to characterize DNA level of germplasm collection in Indonesian Agri-cultural Biotechnology and Genetics Resources Research Institute (IABGRRI). DNA isolation was done as a target, whereas PCR analysis was only done in sweetpotato. The results of PR analysis were random primer's of A01, A04, and B01 could be amplified the DNA on 97 accessions of sweetpotato germplasm with DNA band varied between 1-9.},
    keywords = {Molecular characterization, germplasm, food crops},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_66-74_idahanarida.pdf}
    }
  7. Suardi, D., E. Lubis, and S. Moeljopawiro. 2003. Uji Rumah Kaca untuk Toleransi terhadap Kekeringan Padi Populasi F 7 Persilangan IR64 x IRAT112 (Gajah Mungkur). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 75-83.
    [BibTeX] [Abstract] [PDF: Uji Rumah Kaca untuk Toleransi terhadap Kekeringan Padi Populasi F 7 Persilangan IR64 x IRAT112 (Gajah Mungkur) ]
    The study was conducted in the green house of Balitbiogen in dry season 2002. It was continuation from the study of root penetration ability of BC2F2 (IR64 x IRAT112) lines in 2000. The study was aimed to get rice lines tolerant to drought based on the osmotic adjustment capacity and root penetration ability. Two hundred F7 lines of IR64 x IRAT112 were soaked in PEG 8000 solution of 32.5% concentration The uniform seedling were chosed and planted in plastic pots of 220 cc. The bases of pots were constucted by wax-petrolatum layer of 60% parafin and 40% vaseline mixed, equal to 12 bar strength, and 3 mm thick. Pots experiments were filled by soil, sand and organic matter mixed by ratio of 3.5 : 1.5 : 0.5 by weight. At about 21-25 days after planting, seedlings were planted in pots polybag of 9 kg weight. The experiment was designed by rendomized block design with three replications. The result of the experiment showed that about 12 lines could sprout in PEG 8000 solution with relatively high root penetration ability. About 50% of F7 lines soak in water had relatively good penetration ability and about 3.6% or 17 lines had high root penetration ability. Eight of F7 lines showed of plant height less than 100 cm and the duration less than 115 days. Four of lines i.e. No. 91-2, 5-3, 146-3, and 145-3 were expected to have high yielding potential due to relatively drought tolerant, short stem and duration.
    @article{Suardi03p75,
    title = {{Uji Rumah Kaca untuk Toleransi terhadap Kekeringan Padi Populasi F 7 Persilangan IR64 x IRAT112 (Gajah Mungkur)}},
    author = {D. Suardi and E. Lubis and S. Moeljopawiro},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {75-83},
    year = {2003},
    abstract = { The study was conducted in the green house of Balitbiogen in dry season 2002. It was continuation from the study of root penetration ability of BC2F2 (IR64 x IRAT112) lines in 2000. The study was aimed to get rice lines tolerant to drought based on the osmotic adjustment capacity and root penetration ability. Two hundred F7 lines of IR64 x IRAT112 were soaked in PEG 8000 solution of 32.5% concentration The uniform seedling were chosed and planted in plastic pots of 220 cc. The bases of pots were constucted by wax-petrolatum layer of 60% parafin and 40% vaseline mixed, equal to 12 bar strength, and 3 mm thick. Pots experiments were filled by soil, sand and organic matter mixed by ratio of 3.5 : 1.5 : 0.5 by weight. At about 21-25 days after planting, seedlings were planted in pots polybag of 9 kg weight. The experiment was designed by rendomized block design with three replications. The result of the experiment showed that about 12 lines could sprout in PEG 8000 solution with relatively high root penetration ability. About 50% of F7 lines soak in water had relatively good penetration ability and about 3.6% or 17 lines had high root penetration ability. Eight of F7 lines showed of plant height less than 100 cm and the duration less than 115 days. Four of lines i.e. No. 91-2, 5-3, 146-3, and 145-3 were expected to have high yielding potential due to relatively drought tolerant, short stem and duration.},
    keywords = {Drought, F7 (IR64 x IRAT112) lines, root penetration ability, PEG 8000},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_75-83_didisuardi.pdf}
    }
  8. Susilowati, D. N., Rosmimik, R. Saraswati, R. D. M. Simanungkalit, and L. Gunarto. 2003. Koleksi, Karakterisasi, dan Preservasi Mikroba Penyubur Tanah dan Perombak Bahan Organik. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 84-96.
    [BibTeX] [Abstract] [PDF: Koleksi, Karakterisasi, dan Preservasi Mikroba Penyubur Tanah dan Perombak Bahan Organik ]
    Most of soil fertiliting microbes was preserved at slant agar, and only several microbes have conserved by freeze dried for long-term preservation. Complete characterization included morphology, physiology, biochemical, and genetic analysis have been carried out to soybean Rhizobium. The aim of this research is to characterize to both intergeneric (16 isolates) and intraspecific (19 isolates) Rhizobium and endophytes and phyllosfer microbes. Moreover, we also evaluated some endophyte isolates to produce plant growth promoting hormone (auksin) and to fix nitrogen, but especially for phyllosphere were evaluated the ability to fix nitrogen. We have collected 23 isolates (bacteria and fungy) which is potencial for organic decomposer and 1 yeast with highest cellulolitic activity (0.1298 U/ml). Cellulolitic activity to CMC-ase, $\beta$-glukosidase, and Fp-ase is respectively about 0.129 U/ml, 0.0974 U/ml, and 0.148 U/ml and the protein content is respectively 0.253 mg/ml, 0.198, and 0.276. Enzyme obtained is still crude, so it is important to precipitate with ammonium sulphate. Consentration of the ammonium sulphate that gave the best activity for CMC-ase, $\beta$-glukosidase, and Fp-ase is respectively 60% , 60%, and 70%.
    @article{Susilowati03p84,
    title = {{Koleksi, Karakterisasi, dan Preservasi Mikroba Penyubur Tanah dan Perombak Bahan Organik}},
    author = {D. N. Susilowati and Rosmimik and R. Saraswati and R. D. M. Simanungkalit and L. Gunarto},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {84-96},
    year = {2003},
    abstract = {Most of soil fertiliting microbes was preserved at slant agar, and only several microbes have conserved by freeze dried for long-term preservation. Complete characterization included morphology, physiology, biochemical, and genetic analysis have been carried out to soybean Rhizobium. The aim of this research is to characterize to both intergeneric (16 isolates) and intraspecific (19 isolates) Rhizobium and endophytes and phyllosfer microbes. Moreover, we also evaluated some endophyte isolates to produce plant growth promoting hormone (auksin) and to fix nitrogen, but especially for phyllosphere were evaluated the ability to fix nitrogen. We have collected 23 isolates (bacteria and fungy) which is potencial for organic decomposer and 1 yeast with highest cellulolitic activity (0.1298 U/ml). Cellulolitic activity to CMC-ase, $\beta$-glukosidase, and Fp-ase is respectively about 0.129 U/ml, 0.0974 U/ml, and 0.148 U/ml and the protein content is respectively 0.253 mg/ml, 0.198, and 0.276. Enzyme obtained is still crude, so it is important to precipitate with ammonium sulphate. Consentration of the ammonium sulphate that gave the best activity for CMC-ase, $\beta$-glukosidase, and Fp-ase is respectively 60% , 60%, and 70%.},
    keywords = {collection and characterization, microbe, soil fertiliting, organic decomposer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_84-96_susi.pdf}
    }
  9. Yuniarti, E., D. N. Susilowati, and R. Saraswati. 2003. Koleksi, Karakterisasi, dan Preservasi Mikroba Remediasi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 97-105.
    [BibTeX] [Abstract] [PDF: Koleksi, Karakterisasi, dan Preservasi Mikroba Remediasi ]
    Bioremediation using microbes is needed to overcome environmental problems in the future. A research was carried out to obtain micrbial isolates that have the potential for soil and water bioremediation. Sixty six microbial isolates were collected from Bekasi, Karawang, Solo, Bandung, and Kalimantan Tengah. A number of isolates were able to grow on Peptone Glukosa Yeast extract (PGE) with high contents of high metals, including 24 isolates that grew on PGE containing 1375.4 ppm Mn; 11 isolates grew on PGE containing 1116.9 ppm Fe; 13 isolates grew on PGE containing 176.1 ppm Zn; 33 isolates grew on PGE containing 662.4 ppm Pb; and 39 isolates grew on PGE containing 10 ppm Cd. Two isolates, PG65-06 and MR25-01, tolerant to all the five metals, while the others were only tolerant to some of the metals. Among the 30 isolates characterized, 27 isolates were Gram positive bacteria, while four of them (MT53-01, MP12-05, Bd-3, and Bd-4) were Gram negative bacteria. Of the 16 Gram positive bacterial isolates, 15 of them were rod shaped and produced endospores. These Gram positive bacteria belongs to the genus Bacillus. Based on their morphological, physiological, and biochemichal characteristics, isolates MT77-08Fe, Zn, Pb, Cd, MR1.12-05Zn, Pb, Cd, PG65-06Mn, Fe, ZnPb, Cd, PG97-02Zn, Pb, Cd were Bacillus brevis, PG97-01Zn, Pb, Cd was B. firmus, B2.2Pb, Cd was B. coagulans. One isolate, OM 343, was identified as Saccharomyces sp. Isolates Bacillus spp. PB 138Pb, Cd, Fenol, BB 288Pb, Cd, Fenol, and Saccharomyces sp. OM 343Pb, Cd, Fenol were, able to degrade phenol with phenol hydroxilase activities 1.6 U/ml, 0.8 U/ml, and 0.6 U/ml, respectively. All the isolates were preserved for short term on slant agar medium, and on glycerol cryoprotectant for long term. Twenty four isolates were lyophilized in ampoules for long term preservation.
    @article{Yuniarti03p97,
    title = {{Koleksi, Karakterisasi, dan Preservasi Mikroba Remediasi}},
    author = {E. Yuniarti and D. N. Susilowati and R. Saraswati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {97-105},
    year = {2003},
    abstract = { Bioremediation using microbes is needed to overcome environmental problems in the future. A research was carried out to obtain micrbial isolates that have the potential for soil and water bioremediation. Sixty six microbial isolates were collected from Bekasi, Karawang, Solo, Bandung, and Kalimantan Tengah. A number of isolates were able to grow on Peptone Glukosa Yeast extract (PGE) with high contents of high metals, including 24 isolates that grew on PGE containing 1375.4 ppm Mn; 11 isolates grew on PGE containing 1116.9 ppm Fe; 13 isolates grew on PGE containing 176.1 ppm Zn; 33 isolates grew on PGE containing 662.4 ppm Pb; and 39 isolates grew on PGE containing 10 ppm Cd. Two isolates, PG65-06 and MR25-01, tolerant to all the five metals, while the others were only tolerant to some of the metals. Among the 30 isolates characterized, 27 isolates were Gram positive bacteria, while four of them (MT53-01, MP12-05, Bd-3, and Bd-4) were Gram negative bacteria. Of the 16 Gram positive bacterial isolates, 15 of them were rod shaped and produced endospores. These Gram positive bacteria belongs to the genus Bacillus. Based on their morphological, physiological, and biochemichal characteristics, isolates MT77-08Fe, Zn, Pb, Cd, MR1.12-05Zn, Pb, Cd, PG65-06Mn, Fe, ZnPb, Cd, PG97-02Zn, Pb, Cd were Bacillus brevis, PG97-01Zn, Pb, Cd was B. firmus, B2.2Pb, Cd was B. coagulans. One isolate, OM 343, was identified as Saccharomyces sp. Isolates Bacillus spp. PB 138Pb, Cd, Fenol, BB 288Pb, Cd, Fenol, and Saccharomyces sp. OM 343Pb, Cd, Fenol were, able to degrade phenol with phenol hydroxilase activities 1.6 U/ml, 0.8 U/ml, and 0.6 U/ml, respectively. All the isolates were preserved for short term on slant agar medium, and on glycerol cryoprotectant for long term. Twenty four isolates were lyophilized in ampoules for long term preservation.},
    keywords = {micobial collection and preservation, bioremediation, tolerance to heavy metals},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_97-105_ernyyuniarti.pdf}
    }
  10. Machmud, M., M. A. Suhendar, Y. Suryadi, Jumanto, and M. Sudjadi. 2003. Seleksi dan Karakterisasi Patogen Tanaman. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 106-117.
    [BibTeX] [Abstract] [PDF: Seleksi dan Karakterisasi Patogen Tanaman ]
    Selection and characterization of plant pathogens. Research activities were done in the fiscal year of 2002 to collect, characterize, and preserve isolates of major fungal and bacterial pathogens of food crops from West Java. A number of 201 plant pathogens comprising of 39 fungi and 162 bacteria were collected and identified for their characteristics. The bacterial isolates were preserved in ampoules under freezeo dried conditions at 4 C. Isolates of Ralstonia solanace-arum were also preserved in sterile distilled water at room temperature. The fungal pathogens were preserved on agar medium covered with mineral oil at room temperature. Each collection was preserved in five replicates. Fifty three isolates of plant pathogens including 8 viruses and 45 bacteria which were collected in 2001, were tested for their viabilities. All the isolates were still viable without any changes in their characteristics after one year of storage.
    @article{Machmud03p106,
    title = {{Seleksi dan Karakterisasi Patogen Tanaman}},
    author = {M. Machmud and M. A. Suhendar and Y. Suryadi and Jumanto and M. Sudjadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {106-117},
    year = {2003},
    abstract = {Selection and characterization of plant pathogens. Research activities were done in the fiscal year of 2002 to collect, characterize, and preserve isolates of major fungal and bacterial pathogens of food crops from West Java. A number of 201 plant pathogens comprising of 39 fungi and 162 bacteria were collected and identified for their characteristics. The bacterial isolates were preserved in ampoules under freezeo dried conditions at 4 C. Isolates of Ralstonia solanace-arum were also preserved in sterile distilled water at room temperature. The fungal pathogens were preserved on agar medium covered with mineral oil at room temperature. Each collection was preserved in five replicates. Fifty three isolates of plant pathogens including 8 viruses and 45 bacteria which were collected in 2001, were tested for their viabilities. All the isolates were still viable without any changes in their characteristics after one year of storage.},
    keywords = {selection and characterization, preservation, plant pathogens},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_106-117_machmud_seleksi.pdf}
    }
  11. Machmud, M., M. Sudjadi, and Y. Suryadi. 2003. Seleksi dan Karakterisasi Mikroba Antagonis. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 118-127.
    [BibTeX] [Abstract] [PDF: Seleksi dan Karakterisasi Mikroba Antagonis ]
    In the fiscal year of 2002, research activities were done to collect and preserve microbial antagonists for the control of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani from some areas of West Java. Seventy six antagonists including 35 bacteria (Bacillus subtilis, B. polymixa, Pseudomonas fluorescens), 16 fungi (Trichoderma harzianum), and 25 yeasts (Saccharomyces spp.) were collected and characterized. The bacterial isolates were preserved in ampoules under freeze-dried conditions, while the fungal and yeast isolates were preserved in sterile distilled water. Sixty biological control agents from the 2001 collections have been tested for their viability by growing on agar media. All the isolates that have been preserved under both freeze dried condition and distilled water were still viable and showed good performance after a one-year of storage.
    @article{Machmud03p118,
    title = {{Seleksi dan Karakterisasi Mikroba Antagonis}},
    author = {M. Machmud and M. Sudjadi and Y. Suryadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {118-127},
    year = {2003},
    abstract = {In the fiscal year of 2002, research activities were done to collect and preserve microbial antagonists for the control of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani from some areas of West Java. Seventy six antagonists including 35 bacteria (Bacillus subtilis, B. polymixa, Pseudomonas fluorescens), 16 fungi (Trichoderma harzianum), and 25 yeasts (Saccharomyces spp.) were collected and characterized. The bacterial isolates were preserved in ampoules under freeze-dried conditions, while the fungal and yeast isolates were preserved in sterile distilled water. Sixty biological control agents from the 2001 collections have been tested for their viability by growing on agar media. All the isolates that have been preserved under both freeze dried condition and distilled water were still viable and showed good performance after a one-year of storage.},
    keywords = {collection and preservation, microbial antagonists, plant pests and diseases},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_118-127_machmud_antagonis.pdf}
    }
  12. Susilowati, D. N., R. Saraswati, Elsanti, and E. Yuniarti. 2003. Isolasi dan Seleksi Mikroba Diazotrof Endofitik dan Penghasil Zat Pemacu Tumbuh pada Tanaman Padi dan Jagung. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 128-144.
    [BibTeX] [Abstract] [PDF: Isolasi dan Seleksi Mikroba Diazotrof Endofitik dan Penghasil Zat Pemacu Tumbuh pada Tanaman Padi dan Jagung ]
    A number of endophytic bacteria had a significant potency on N2 fixing activuty and IAA production. About 142 endophytic bacteria have been obtained from rice (95 isolates) and corn (47 isolates) isolated from West Java, East Java, and South Kalimantan. Based on N2 fixing activuty and IAA production, we have obtained 5 superior isolates from rice, that is BCr 1.2 (IAA 4.530 ppm), BCbd 1.3 (IAA 5.363 ppm; N2 fixing activity 0.0116 umol/hour/tube), APK 2.4 (IAA 2.818 ppm; N2 fixing activity 2.683 umol/hour/tube), BCr 2.3 (IAA 1.539 ppm; N2 fixing activity 1.9495 umol/hour/tube), and BCr 2.1 (IAA 1.730 ppm; N2 fixing activity 1.280 umol/hour/tube). Moreover we have selected 5 superior isolates form corn, that is JCbd 2.1 (IAA 5.897 ppm; N2 fixing activity 0.059 umol/hour/ tube), JCmg 3.3 (N2 fixing activity 0.039 umol/hour/tube), JKW 1.1B (IAA 1.036 ppm), JSHC 2.4 (N2 fixing activity 0.219 umol/hour/tube), and JLkCN 2.3 (IAA 0.677 ppm; N2 fixing activity 0.469 umol/hour/tube). Inoculation the endophytic bacteria in laboratorium scale visually increased the development of primary and hairy root of corn. Inoculation with JCBD 2.1 and JLkCN 2.3 isolates significantly contributed on the height of plant growth compared with control. However, inoculation treatment did not significantly response on stem diameter growth, number of leaf, wet and dry weight of shoot, dry wet of root, chlorophyll content, and total nitrogen. Dosage 25% of anorganic nitrogen (N1) into soil did not significantly response compared with control (N0), but dosage 50% (N2), 75% (N3), and 100% (N4) of anorganic nitrogen gave significantly effect on total nitrogen compared with control (N0). But, this factor did not significantly response on the height of plant growth, stem diameter, number of leaf, wet and dry weight of shoot, dry weight of root, and chlorophyll content. No interaction between inoculation factor with the level of nitrogen given.
    @article{Susilowati03p128,
    title = {{Isolasi dan Seleksi Mikroba Diazotrof Endofitik dan Penghasil Zat Pemacu Tumbuh pada Tanaman Padi dan Jagung}},
    author = {D. N. Susilowati and R. Saraswati and Elsanti and E. Yuniarti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {128-144},
    year = {2003},
    abstract = {A number of endophytic bacteria had a significant potency on N2 fixing activuty and IAA production. About 142 endophytic bacteria have been obtained from rice (95 isolates) and corn (47 isolates) isolated from West Java, East Java, and South Kalimantan. Based on N2 fixing activuty and IAA production, we have obtained 5 superior isolates from rice, that is BCr 1.2 (IAA 4.530 ppm), BCbd 1.3 (IAA 5.363 ppm; N2 fixing activity 0.0116 umol/hour/tube), APK 2.4 (IAA 2.818 ppm; N2 fixing activity 2.683 umol/hour/tube), BCr 2.3 (IAA 1.539 ppm; N2 fixing activity 1.9495 umol/hour/tube), and BCr 2.1 (IAA 1.730 ppm; N2 fixing activity 1.280 umol/hour/tube). Moreover we have selected 5 superior isolates form corn, that is JCbd 2.1 (IAA 5.897 ppm; N2 fixing activity 0.059 umol/hour/ tube), JCmg 3.3 (N2 fixing activity 0.039 umol/hour/tube), JKW 1.1B (IAA 1.036 ppm), JSHC 2.4 (N2 fixing activity 0.219 umol/hour/tube), and JLkCN 2.3 (IAA 0.677 ppm; N2 fixing activity 0.469 umol/hour/tube). Inoculation the endophytic bacteria in laboratorium scale visually increased the development of primary and hairy root of corn. Inoculation with JCBD 2.1 and JLkCN 2.3 isolates significantly contributed on the height of plant growth compared with control. However, inoculation treatment did not significantly response on stem diameter growth, number of leaf, wet and dry weight of shoot, dry wet of root, chlorophyll content, and total nitrogen. Dosage 25% of anorganic nitrogen (N1) into soil did not significantly response compared with control (N0), but dosage 50% (N2), 75% (N3), and 100% (N4) of anorganic nitrogen gave significantly effect on total nitrogen compared with control (N0). But, this factor did not significantly response on the height of plant growth, stem diameter, number of leaf, wet and dry weight of shoot, dry weight of root, and chlorophyll content. No interaction between inoculation factor with the level of nitrogen given.},
    keywords = {Isolation, selection, endophytic diazotroph, rice, corn},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_128-144_susilowati_isolasi.pdf}
    }
  13. Somantri, I. H., A. D. Ambarwati, I. S. Dewi, A. Apriana, and T. J. Santoso. 2003. Transformasi Padi Japonica (T-309) dan Indica dengan Gen CryIA(b). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 145-151.
    [BibTeX] [Abstract] [PDF: Transformasi Padi Japonica (T-309) dan Indica dengan Gen CryIA(b) ]
    The research was carried out at the Genetic Engineering Laboratory, Molecular Biology Division, IABGRRI, Bogor, in 2002. The aim of the research was to obtain transgenic rice containing cryIA(b) gene. Materials used in this research were Sintanur and Cisadane (Indica) and Taipei-309 (Japonica). The transfor-mation technique was carried out by means of particle bombardment using pUBB plasmid that had been cotransformed with pRQ6 plasmid at 4 : 1 molarity. Plasmid pUBB contains cryIA(b) gene, while plasmid pRQ6 contains two genes that are gus gene as reporter gene and hph gene as selectable marker. 280 plants of putative transgenic rice originated from Taipei-309 could be obtained via NK4N2 regeneration medium, while only one plant of putative transgenic rice originated from Cisadane could be obtained via MSP regeneration medium. This result indicated that NK4N2 and MSP medium could be used as regeneration media. At present, Sintanur could not be induced to produce calli. The remaining calli of Taipei-309 and Cisadane were still on regeneration media. First transformed plants (T0) surviving in the accli-matization phase were moved to a green house for further evaluation.
    @article{Somantri03p145,
    title = {{Transformasi Padi Japonica (T-309) dan Indica dengan Gen CryIA(b)}},
    author = {I. H. Somantri and A. D. Ambarwati and I. S. Dewi and A. Apriana and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {145-151},
    year = {2003},
    abstract = {The research was carried out at the Genetic Engineering Laboratory, Molecular Biology Division, IABGRRI, Bogor, in 2002. The aim of the research was to obtain transgenic rice containing cryIA(b) gene. Materials used in this research were Sintanur and Cisadane (Indica) and Taipei-309 (Japonica). The transfor-mation technique was carried out by means of particle bombardment using pUBB plasmid that had been cotransformed with pRQ6 plasmid at 4 : 1 molarity. Plasmid pUBB contains cryIA(b) gene, while plasmid pRQ6 contains two genes that are gus gene as reporter gene and hph gene as selectable marker. 280 plants of putative transgenic rice originated from Taipei-309 could be obtained via NK4N2 regeneration medium, while only one plant of putative transgenic rice originated from Cisadane could be obtained via MSP regeneration medium. This result indicated that NK4N2 and MSP medium could be used as regeneration media. At present, Sintanur could not be induced to produce calli. The remaining calli of Taipei-309 and Cisadane were still on regeneration media. First transformed plants (T0) surviving in the accli-matization phase were moved to a green house for further evaluation.},
    keywords = {rice transformation, pubb, particle bombardment, prq6},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_145-151_idahanarida_transformasi.pdf}
    }
  14. Santoso, T. J., A. Apriana, A. D. Ambarwati, I. S. Dewi, and I. H. Somantri. 2003. Analisis Molekuler Lanjutan Tanaman Putatif Transgenik Padi Gen CryIA Generasi T0, T1, T2, dan T3. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 152-158.
    [BibTeX] [Abstract] [PDF: Analisis Molekuler Lanjutan Tanaman Putatif Transgenik Padi Gen CryIA Generasi T0, T1, T2, dan T3 ]
    Molecular analysis of transgenic plants is to identify the transgene and its expression. The experiment reported here is intended to confirm the integrity of cryIAb gene. The identification of the transgene can be carried out through a number of molecular techniques. One of the techniques is Polymerase Chain Reaction (PCR) analysis. In 2002, the continuation of PCR analysis was conducted in the putative transgenic rice cv. Taipei-309. The PCR analysis VI and VIII were conducted for 44 plants (T0) generation while the PCR analysis VII was conducted for 21 plants (T1, T2, and T3 generation). The result showed that PCR VI did not identify and plants carrying cryIAb gene while PCR VIII found 3 plants containing the transgene. On the other hand, PCR VII identify 10 plants that contained the transgene and showed moderate to extremely high levels of resistance to stem borer.
    @article{Santoso03p152,
    title = {{Analisis Molekuler Lanjutan Tanaman Putatif Transgenik Padi Gen CryIA Generasi T0, T1, T2, dan T3}},
    author = {T. J. Santoso and A. Apriana and A. D. Ambarwati and I. S. Dewi and I. H. Somantri},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {152-158},
    year = {2003},
    abstract = {Molecular analysis of transgenic plants is to identify the transgene and its expression. The experiment reported here is intended to confirm the integrity of cryIAb gene. The identification of the transgene can be carried out through a number of molecular techniques. One of the techniques is Polymerase Chain Reaction (PCR) analysis. In 2002, the continuation of PCR analysis was conducted in the putative transgenic rice cv. Taipei-309. The PCR analysis VI and VIII were conducted for 44 plants (T0) generation while the PCR analysis VII was conducted for 21 plants (T1, T2, and T3 generation). The result showed that PCR VI did not identify and plants carrying cryIAb gene while PCR VIII found 3 plants containing the transgene. On the other hand, PCR VII identify 10 plants that contained the transgene and showed moderate to extremely high levels of resistance to stem borer.},
    keywords = {rice, oryza sativa, cryiab gene, polymerase chain reaction, pcr},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_152-158_trijoko_analisis.pdf}
    }
  15. Dewi, I. S., I. H. Somantri, D. Damayanti, A. Apriana, and T. J. Santoso. 2003. Bioasai Lanjutan Tanaman Padi Transgenik Putatif CryIA(b) Generasi T1, T2, dan T3. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 159-166.
    [BibTeX] [Abstract] [PDF: Bioasai Lanjutan Tanaman Padi Transgenik Putatif CryIA(b) Generasi T1, T2, dan T3 ]
    The success in transgenic plants production occurs when plants express the inserted gene and interest new phenotype appears. The objective of the research is to obtain putative transgenic rice (T1, T2, and T3 generation) resistant to stem borer. The material used in this research were T1-T-5B, T1-T-5H, T2-B5-T1-C7, T2-B6-T1-C7, T2B24-T1-C7, T3-A1-T-4B, T3-A2-T-4B, and T3-A3-T-4B selected from generation of Taipei-309 putative transgenic rice. At vegetative stage, observation on sundep (deadhearts) indicated that 13 plants were highly resistant, 5 plants were resistant, 9 plants were moderately resistant, 12 plants were moderately susceptible, 5 plants were susceptible and 2 plants were highly susceptible. At generative stage, observation on beluk (white-heads) indicated that 19 plants were highly resistant, 2 plants were resistant, 5 plants were moderately resistant, 10 plants were moderately susceptible, 5 plants were susceptible and 5 plants were highly susceptible. Plants resistant to yellow stemborer at vegetative as well as generative stage were 1 plant from T1-T-5B, 2 plants from T1-T-5H, 10 plants from T2-T1-C7, and 4 plants from T3-T-4B.
    @article{Dewi03p159,
    title = {{Bioasai Lanjutan Tanaman Padi Transgenik Putatif CryIA(b) Generasi T1, T2, dan T3}},
    author = {I. S. Dewi and I. H. Somantri and D. Damayanti and A. Apriana and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {159-166},
    year = {2003},
    abstract = {The success in transgenic plants production occurs when plants express the inserted gene and interest new phenotype appears. The objective of the research is to obtain putative transgenic rice (T1, T2, and T3 generation) resistant to stem borer. The material used in this research were T1-T-5B, T1-T-5H, T2-B5-T1-C7, T2-B6-T1-C7, T2B24-T1-C7, T3-A1-T-4B, T3-A2-T-4B, and T3-A3-T-4B selected from generation of Taipei-309 putative transgenic rice. At vegetative stage, observation on sundep (deadhearts) indicated that 13 plants were highly resistant, 5 plants were resistant, 9 plants were moderately resistant, 12 plants were moderately susceptible, 5 plants were susceptible and 2 plants were highly susceptible. At generative stage, observation on beluk (white-heads) indicated that 19 plants were highly resistant, 2 plants were resistant, 5 plants were moderately resistant, 10 plants were moderately susceptible, 5 plants were susceptible and 5 plants were highly susceptible. Plants resistant to yellow stemborer at vegetative as well as generative stage were 1 plant from T1-T-5B, 2 plants from T1-T-5H, 10 plants from T2-T1-C7, and 4 plants from T3-T-4B.},
    keywords = {transgenic rice, bioassay, stemborer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_159-166_iswari_bioassai.pdf}
    }
  16. Sutrisno, Saptowo J. Pardal, Diani Damayanti, Hermanvand Riri M. Sundasari, and Endang Ibrahim. 2003. Bioasai Tanaman Kedelai Transgenik pinII terhadap Hama Penggerek Polong (Etiella zinckenella, Treitschke). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 167-172.
    [BibTeX] [Abstract] [PDF: Bioasai Tanaman Kedelai Transgenik pinII terhadap Hama Penggerek Polong (Etiella zinckenella, Treitschke) ]
    Bioassay on R3 generation of transgenic soybean plants to pod borer (Etiella zinckenella, Tr.) larvae has been conducted at Biosafety Containment of IABGRRI in year 2002 the APBN research budgets. The objective of this assay was to obtain transgenic soybean plants resistant to pod borer. Transgenic soybean seeds of WP1 (4 plants) and WP2 (6 plants) events were planted in greenhouse of Biosafety Containment. On fifty days after plantation, each plant was infested with 5 pair of pod borer imago, and then covered with plastic cover (to avoid escaped insects). Observation was carried out on pods before harvesting. The observation was focus on the percentage pod and seed damages. Result indicated that all 10 transgenic soybean plants were not so resistant to pod borer larvae compared to control plants. The lower pod and seed damages attacked by pod borer showed on event WP1-1-1 (51.8% and 44.3%). While the highest pod and seed damages found on event WP1-1-4 (100% and 100%). This result should be confirm with molecular analysis to ensure the transgenic plants.
    @article{Sutrisno03p167,
    title = {{Bioasai Tanaman Kedelai Transgenik pinII terhadap Hama Penggerek Polong (Etiella zinckenella, Treitschke)}},
    author = {Sutrisno and Saptowo J. Pardal and Diani Damayanti and M. Hermanvand Riri Sundasari and Endang Ibrahim},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {167-172},
    year = {2003},
    abstract = {Bioassay on R3 generation of transgenic soybean plants to pod borer (Etiella zinckenella, Tr.) larvae has been conducted at Biosafety Containment of IABGRRI in year 2002 the APBN research budgets. The objective of this assay was to obtain transgenic soybean plants resistant to pod borer. Transgenic soybean seeds of WP1 (4 plants) and WP2 (6 plants) events were planted in greenhouse of Biosafety Containment. On fifty days after plantation, each plant was infested with 5 pair of pod borer imago, and then covered with plastic cover (to avoid escaped insects). Observation was carried out on pods before harvesting. The observation was focus on the percentage pod and seed damages. Result indicated that all 10 transgenic soybean plants were not so resistant to pod borer larvae compared to control plants. The lower pod and seed damages attacked by pod borer showed on event WP1-1-1 (51.8% and 44.3%). While the highest pod and seed damages found on event WP1-1-4 (100% and 100%). This result should be confirm with molecular analysis to ensure the transgenic plants.},
    keywords = {bioassay, r3 transgenic soybean plants, resistance, pod borer, etiella zinckenella, tr},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_167-172_sutrisno_bioassai.pdf}
    }
  17. Manzila, I., Jumanto, T. I. R. Utami, Wawan, and A. Wardoyo. 2003. Analisis Molekuler Gen CP-PStV pada Tanaman Kacang Tanah Transgenik. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 173-178.
    [BibTeX] [Abstract] [PDF: Analisis Molekuler Gen CP-PStV pada Tanaman Kacang Tanah Transgenik ]
    A research to analyze molecular existances of coat protein gen of PStV (CP-PStV) in second generation (R2) of transgenic peanut (Arachis hypogaea L.) plant based on polymerase chain reaction (PCR), was carried out in the screen house and laboratory virology of Agricultural Research and Development Biotechnology and Genetic Resources, Bogor in 2002. Two main step of the research were: 1) Isolation of total DNA of the seventeen genotypes to be analysed and one control (non transgenic) of young one week-old leaves; 2) CP-PStV gene confirmation with PCR technique. PCR optimization was carried out against 5 putative plants resistant to PStV which was obtained from bioassay result. Presence of CP-PStV gene was detected using pair of spesific primer PST1 and PST2 with PCR and electrophoresis gel. It was used DNA of non transgenic plants, Plasmid DNA containing CP-PStV gene of size +300 bp, and BPH plasmid DNA size of 500 bp as control. Results of the electrophoregraph analysis revealed presence of three putative transgenic plants positive contained CP-PStV gene with band at ±300 bp. Those genotypes were R2Dd1.4, R2Dd 1.2, and Cp4Dd2.
    @article{Manzila03p173,
    title = {{Analisis Molekuler Gen CP-PStV pada Tanaman Kacang Tanah Transgenik}},
    author = {I. Manzila and Jumanto and T. I. R. Utami and Wawan and A. Wardoyo},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {173-178},
    year = {2003},
    abstract = {A research to analyze molecular existances of coat protein gen of PStV (CP-PStV) in second generation (R2) of transgenic peanut (Arachis hypogaea L.) plant based on polymerase chain reaction (PCR), was carried out in the screen house and laboratory virology of Agricultural Research and Development Biotechnology and Genetic Resources, Bogor in 2002. Two main step of the research were: 1) Isolation of total DNA of the seventeen genotypes to be analysed and one control (non transgenic) of young one week-old leaves; 2) CP-PStV gene confirmation with PCR technique. PCR optimization was carried out against 5 putative plants resistant to PStV which was obtained from bioassay result. Presence of CP-PStV gene was detected using pair of spesific primer PST1 and PST2 with PCR and electrophoresis gel. It was used DNA of non transgenic plants, Plasmid DNA containing CP-PStV gene of size +300 bp, and BPH plasmid DNA size of 500 bp as control. Results of the electrophoregraph analysis revealed presence of three putative transgenic plants positive contained CP-PStV gene with band at ±300 bp. Those genotypes were R2Dd1.4, R2Dd 1.2, and Cp4Dd2.},
    keywords = {molecular analysis, cp-pstv gene, r2 transgenic peanut plant},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_173-178_ifa_analisis.pdf}
    }
  18. Manzila, I., Jumanto, A. Wardoyo, and Wawan. 2003. Bioasai Tanaman Kacang Tanah Transgenik terhadap Virus Bilur Kacang Tanah (PStV). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 179-183.
    [BibTeX] [Abstract] [PDF: Bioasai Tanaman Kacang Tanah Transgenik terhadap Virus Bilur Kacang Tanah (PStV) ]
    The experiment of bioassay to peanut transgenic R1plant was conducted at Lab virology and glass house of Agricultural research of biotechnology and genetic resources Bogor in FY 2002. The output of transgenic plant production was obtained by sucsesfull gene expression of the insert gene and new plant phenotype i.e, plant resistant to PStV. One of the method was by using direct assay (bioassay). In this research it has been obtained transgenic peanut containing CP-PStV gene. Result of putative transgenic R1 plant, 3 category has been obtained i.e 8 putative plants resistant to PStV (R2Dd1.(1), R2Dd1.(3), R2Dd1.(4), R2Dd2.(2), R2Dd.(5), Eel 2, Cp4Dd2.2, and Cp4Dd2.3) showing disease intensity 0%, 5 putative plants Moderately resistant (Eel 5, Eel 6, R2Dd2.(4), R2Dd2.(3) and R2Dd1.1) with intensity of 33.33%, and 5 putative plant susceptible (R2Dd1.2, R2Dd2.(1), Eel 1, Eel 3, and control) showing disease intensity of 66.66%.
    @article{Manzila03p179,
    title = {{Bioasai Tanaman Kacang Tanah Transgenik terhadap Virus Bilur Kacang Tanah (PStV)}},
    author = {I. Manzila and Jumanto and A. Wardoyo and Wawan},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {179-183},
    year = {2003},
    abstract = {The experiment of bioassay to peanut transgenic R1plant was conducted at Lab virology and glass house of Agricultural research of biotechnology and genetic resources Bogor in FY 2002. The output of transgenic plant production was obtained by sucsesfull gene expression of the insert gene and new plant phenotype i.e, plant resistant to PStV. One of the method was by using direct assay (bioassay). In this research it has been obtained transgenic peanut containing CP-PStV gene. Result of putative transgenic R1 plant, 3 category has been obtained i.e 8 putative plants resistant to PStV (R2Dd1.(1), R2Dd1.(3), R2Dd1.(4), R2Dd2.(2), R2Dd.(5), Eel 2, Cp4Dd2.2, and Cp4Dd2.3) showing disease intensity 0%, 5 putative plants Moderately resistant (Eel 5, Eel 6, R2Dd2.(4), R2Dd2.(3) and R2Dd1.1) with intensity of 33.33%, and 5 putative plant susceptible (R2Dd1.2, R2Dd2.(1), Eel 1, Eel 3, and control) showing disease intensity of 66.66%.},
    keywords = {r1 transgenic plant, bioassay, pstv, resistance},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_179-183_ifa_bioassay.pdf}
    }
  19. Ambarwati, A. D., A. Sisharmini, T. J. Santoso, M. Herman, and Minantyorini. 2003. Transformasi Ubi Jalar dengan Gen pinII dan Gen CPSPFMV melalui Agrobacterium tumefaciens. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 184-192.
    [BibTeX] [Abstract] [PDF: Transformasi Ubi Jalar dengan Gen pinII dan Gen CPSPFMV melalui Agrobacterium tumefaciens ]
    The development of transgenic sweet potato resistant to pest and virus diseases can be done by genetic engineering through transformation techniques. In the year 2002 the experiment on sweet potato transformation was conducted to obtain their resistance to weevil Cylas formicarius and Sweet Potato Feathery Mottle Virus (SPFMV). Transformation was performed by introducing proteinase inhibitor (pinII) or coat protein (CP)-SPFMV genes with transformation techniques of Agrobacterium tumefaciens. Transformation through A. tumefaciens used LBA4404 strain containing pGApin (pinII, nptII) or binary plasmid pMON10574/pMON10575 containing CPSPFMV, gus, and nptII genes. Explants from petiole and leaf pieces of sweet potato cv. Jewel and Bis 182-81 were used as target tissue. The results indicate that 100 µM of acetosyringone concentration and 60 minutes inoculation times could increase transformation efficiency, which the mean of blue spot was 2.9-4.4 per explant both on Jewel and Bis 182-81. R2 media (MS + 0.2 mg/l 2ip) more responsive in regenerating petiole explant transformed with pinII gene through organogenesis pathways. Pre treatment of explant before transformation by wounding with carborundum was needed to increase transformation efficiency on petiole explants. Transformation result in 5 putative transgenic plants of Jewel transformed with CP-SPFMV gene and 21 putative transgenic plants transformed with pinII gene.
    @article{Ambarwati03p184,
    title = {{Transformasi Ubi Jalar dengan Gen pinII dan Gen CPSPFMV melalui Agrobacterium tumefaciens}},
    author = {A. D. Ambarwati and A. Sisharmini and T. J. Santoso and M. Herman and Minantyorini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {184-192},
    year = {2003},
    abstract = {The development of transgenic sweet potato resistant to pest and virus diseases can be done by genetic engineering through transformation techniques. In the year 2002 the experiment on sweet potato transformation was conducted to obtain their resistance to weevil Cylas formicarius and Sweet Potato Feathery Mottle Virus (SPFMV). Transformation was performed by introducing proteinase inhibitor (pinII) or coat protein (CP)-SPFMV genes with transformation techniques of Agrobacterium tumefaciens. Transformation through A. tumefaciens used LBA4404 strain containing pGApin (pinII, nptII) or binary plasmid pMON10574/pMON10575 containing CPSPFMV, gus, and nptII genes. Explants from petiole and leaf pieces of sweet potato cv. Jewel and Bis 182-81 were used as target tissue. The results indicate that 100 µM of acetosyringone concentration and 60 minutes inoculation times could increase transformation efficiency, which the mean of blue spot was 2.9-4.4 per explant both on Jewel and Bis 182-81. R2 media (MS + 0.2 mg/l 2ip) more responsive in regenerating petiole explant transformed with pinII gene through organogenesis pathways. Pre treatment of explant before transformation by wounding with carborundum was needed to increase transformation efficiency on petiole explants. Transformation result in 5 putative transgenic plants of Jewel transformed with CP-SPFMV gene and 21 putative transgenic plants transformed with pinII gene.},
    keywords = {transformation, sweet potato, pinii gene, cp-spfmv gene},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_184-192_dinar_transformasi.pdf}
    }
  20. Abdullah, B., M. Bustamam, T. S. Silitonga, Bahagiawati, D. Suardi, J. Prasetiyono, Tasliah, M. Amir, and A. Nasution. 2003. Evaluasi Ketahanan Spesies Padi Liar terhadap Cekaman Biotik dan Abiotik dan Karakterisasi dengan Menggunakan Markah Mikrosatelit. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 202-216.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Spesies Padi Liar terhadap Cekaman Biotik dan Abiotik dan Karakterisasi dengan Menggunakan Markah Mikrosatelit ]
    Evaluation of wild species of rice resistance to biotic and abiotic and characterization using microsatellite markers was conducted in 2002. The investigation was aimed to obtain gene sources for resistance against biotic and abiotic stresses. Fifteen species consisted of 76 accessions of wild species and 3 accessions of African rice were evaluated for their resistance against bioteic (bacterial blight, blast, and brown plant hopper) and abioteic (drought) stresses; and also analyzed for fingerprinting using microsatellite markers. Fifty seven accessions of wild species showed their resistance against bacterial blight, 25 accessions resistant to 2 strains IV and VIII and 32 resistance against strain IV. The resistant accessions were mostly O. officinalis. Seven accessions showed resistant and 3 showed moderately resistant to race 041 of Indramayu blast. The resistant accessions were belong to O. officinalis. Twenty-nine accessions showed resistant and 7 moderately resistant to brown plant hopper, most of them were also belong to O. officinalis Two accessions of wild species, i.e. O. nivara 105623 and O. glaberrima, 101297 showed tolerant to drought as they had good root penetration ability under combination of vaselin and paraffin test in the laboratory. DNA isolation was succeded. Out of 24 microsatelitte markers, fungerprint. The wild species tested showed polymorfisms between species as well as accessions.
    @article{Abdullah03p202,
    title = {{Evaluasi Ketahanan Spesies Padi Liar terhadap Cekaman Biotik dan Abiotik dan Karakterisasi dengan Menggunakan Markah Mikrosatelit}},
    author = {B. Abdullah and M. Bustamam and T. S. Silitonga and Bahagiawati and D. Suardi and J. Prasetiyono and Tasliah and M. Amir and A. Nasution},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {202-216},
    year = {2003},
    abstract = {Evaluation of wild species of rice resistance to biotic and abiotic and characterization using microsatellite markers was conducted in 2002. The investigation was aimed to obtain gene sources for resistance against biotic and abiotic stresses. Fifteen species consisted of 76 accessions of wild species and 3 accessions of African rice were evaluated for their resistance against bioteic (bacterial blight, blast, and brown plant hopper) and abioteic (drought) stresses; and also analyzed for fingerprinting using microsatellite markers. Fifty seven accessions of wild species showed their resistance against bacterial blight, 25 accessions resistant to 2 strains IV and VIII and 32 resistance against strain IV. The resistant accessions were mostly O. officinalis. Seven accessions showed resistant and 3 showed moderately resistant to race 041 of Indramayu blast. The resistant accessions were belong to O. officinalis. Twenty-nine accessions showed resistant and 7 moderately resistant to brown plant hopper, most of them were also belong to O. officinalis Two accessions of wild species, i.e. O. nivara 105623 and O. glaberrima, 101297 showed tolerant to drought as they had good root penetration ability under combination of vaselin and paraffin test in the laboratory. DNA isolation was succeded. Out of 24 microsatelitte markers, fungerprint. The wild species tested showed polymorfisms between species as well as accessions.},
    keywords = {wild species of rice, microsatelitte, biotic, abiotic, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_202-216_buangabdullah_evaluasi.pdf}
    }
  21. Suhartini, T., T. S. Silitonga, and B. Abdullah. 2003. Pembentukan Populasi Interspesifik Padi melalui Kultur Embrio secara In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 217-225.
    [BibTeX] [Abstract] [PDF: Pembentukan Populasi Interspesifik Padi melalui Kultur Embrio secara In Vitro ]
    Interspecific crossing was conducted in the year 2002 between Pelita 1-1, Way Rarem, IR64 as cultivated rice and wild rice Oryza minuta 101141, O. punctata 101409. O.punctata 100892, O. australiensis 105219, O. australiensis 105623, O, officinalis 100896, O. officinalis 100878, and O. rufipogon 102186. Back cross method applied following embryo rescue. The objective of these activities were to introgress genes for resistant to brown plant hopper (BPH), bacterial blight (BB), blast and tolerant to drought to cultivate released varieties and to develop new genetic diversity rice breeding program improvement. From 24 crosses only 14 have succeeded in producing F1 plants. A total of 170 plants f1 or 1.2% produced by embryo rescue. Some of cross combination did not produced embryo or F1 plants especially for 2 crossing with O. australiensis and O. officinalis except for IR64 /O. officinalis. The back 2 2 crossing (BC1) was done for F1 plant of Way Rarem /O. punctata 101409, IR64 /O. punctata 2 2 2 101409, IR64 /O. punctata 100892, IR64 /O. minuta 101141, IR64 /O. officinalis 100878, 2 2 2 IR64 /O. officinalis 100896, Pelita 1-1 /O. punctata 101409, Pelita 1-1 /O. punctata 100892, 2 and Pelita 1-1 /O. minuta 10114. Total panicle emasculated range between 2-62 with total 2 spikelets pollinated were 128 to 7175. The F1BC1 was obtained from IR64 /O. punctata 101409 and from crossing of improved varieties with O. rufipogon 102186.
    @article{Suhartini03p217,
    title = {{Pembentukan Populasi Interspesifik Padi melalui Kultur Embrio secara In Vitro}},
    author = {T. Suhartini and T. S. Silitonga and B. Abdullah},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {217-225},
    year = {2003},
    abstract = {Interspecific crossing was conducted in the year 2002 between Pelita 1-1, Way Rarem, IR64 as cultivated rice and wild rice Oryza minuta 101141, O. punctata 101409. O.punctata 100892, O. australiensis 105219, O. australiensis 105623, O, officinalis 100896, O. officinalis 100878, and O. rufipogon 102186. Back cross method applied following embryo rescue. The objective of these activities were to introgress genes for resistant to brown plant hopper (BPH), bacterial blight (BB), blast and tolerant to drought to cultivate released varieties and to develop new genetic diversity rice breeding program improvement. From 24 crosses only 14 have succeeded in producing F1 plants. A total of 170 plants f1 or 1.2% produced by embryo rescue. Some of cross combination did not produced embryo or F1 plants especially for 2 crossing with O. australiensis and O. officinalis except for IR64 /O. officinalis. The back 2 2 crossing (BC1) was done for F1 plant of Way Rarem /O. punctata 101409, IR64 /O. punctata 2 2 2 101409, IR64 /O. punctata 100892, IR64 /O. minuta 101141, IR64 /O. officinalis 100878, 2 2 2 IR64 /O. officinalis 100896, Pelita 1-1 /O. punctata 101409, Pelita 1-1 /O. punctata 100892, 2 and Pelita 1-1 /O. minuta 10114. Total panicle emasculated range between 2-62 with total 2 spikelets pollinated were 128 to 7175. The F1BC1 was obtained from IR64 /O. punctata 101409 and from crossing of improved varieties with O. rufipogon 102186.},
    keywords = {interspecific crossing, rice, embryo rescue, in vitro},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_217-225_tintin_pembentukan.pdf}
    }
  22. Dewi, I. S., A. Apriana, I. H. Somantri, A. D. Ambarwati, Suwarno, and Minantyorini. 2003. Perbaikan Galur Mandul Jantan dan Pemulih Kesuburan melalui Kultur Antera. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 226-235.
    [BibTeX] [Abstract] [PDF: Perbaikan Galur Mandul Jantan dan Pemulih Kesuburan melalui Kultur Antera ]
    Anther culture technique was conducted to regenerate doubled haploid plants directly from F1 plants or other selected segregate plant materials. Therefore, this technique can be used to accelerate breeding cycle. In this research, anther culture of F1 from maintainer or restorer lines x varieties were conducted on two induction/regeneration media, namely A1-A1R (induction medium N6/regener-ation medium MS supplemented with 10-3M putresin) and A2-A2R (induction medium N6 supplemented with 2 mg/l 2,4-D). The purpose of this research was: (1) to obtain seeds from crossing of maintainer and restorer lines with high yielding varieties having interest good traits, such as high yield, tolerance to biotic and abiotic stresses and good eating quality; (2) to obtain maintainer and restorer lines having the interest traits through anther culture. The results from 8 crosses of maintainer lines x released varieties were 218 seeds from IR62829B x Sintanur, 66 seeds from IR58025B x Sintanur, 643 seeds from IR62829B x Ciherang, 165 seeds from IR58025B x Ciherang, 528 seeds from IR62829B x IR64, 64 seeds from IR58025B x IR64, 360 seeds from IR62829B x Membe- ramo, and 74 seeds from IR58025B x Memberamo, while from restorer lines x released varieties were 52 seeds from IR53942R x Ciherang, 316 seeds from IR53942R x IR64, 56 seeds from IR53942R x Sintanur, 297 seeds from IR53942R x Memberamo, 177 seeds from BR827-35R x Ciherang, 308 seeds from BR827-35R x IR64, 91 seeds from BR827-35R x Sintanur, and 273 seeds from BR827-35R x Memberamo. A1-A1R medium was more efficient in the production of calli, plantlet formation from calli, green and albino plantlet and total number of plantlet than A2-A2R medium. We obtained 43 maintainer lines originated from anther culture of F1 (IR58025B x Sintanur and IR62829B x Ciherang) and 55 restorer lines originated from anther culture F1 (IR53942R x Ciherang and BR827-35R x Sintanur).
    @article{Dewi03p226,
    title = {{Perbaikan Galur Mandul Jantan dan Pemulih Kesuburan melalui Kultur Antera}},
    author = {I. S. Dewi and A. Apriana and I. H. Somantri and A. D. Ambarwati and Suwarno and Minantyorini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {226-235},
    year = {2003},
    abstract = { Anther culture technique was conducted to regenerate doubled haploid plants directly from F1 plants or other selected segregate plant materials. Therefore, this technique can be used to accelerate breeding cycle. In this research, anther culture of F1 from maintainer or restorer lines x varieties were conducted on two induction/regeneration media, namely A1-A1R (induction medium N6/regener-ation medium MS supplemented with 10-3M putresin) and A2-A2R (induction medium N6 supplemented with 2 mg/l 2,4-D). The purpose of this research was: (1) to obtain seeds from crossing of maintainer and restorer lines with high yielding varieties having interest good traits, such as high yield, tolerance to biotic and abiotic stresses and good eating quality; (2) to obtain maintainer and restorer lines having the interest traits through anther culture. The results from 8 crosses of maintainer lines x released varieties were 218 seeds from IR62829B x Sintanur, 66 seeds from IR58025B x Sintanur, 643 seeds from IR62829B x Ciherang, 165 seeds from IR58025B x Ciherang, 528 seeds from IR62829B x IR64, 64 seeds from IR58025B x IR64, 360 seeds from IR62829B x Membe- ramo, and 74 seeds from IR58025B x Memberamo, while from restorer lines x released varieties were 52 seeds from IR53942R x Ciherang, 316 seeds from IR53942R x IR64, 56 seeds from IR53942R x Sintanur, 297 seeds from IR53942R x Memberamo, 177 seeds from BR827-35R x Ciherang, 308 seeds from BR827-35R x IR64, 91 seeds from BR827-35R x Sintanur, and 273 seeds from BR827-35R x Memberamo. A1-A1R medium was more efficient in the production of calli, plantlet formation from calli, green and albino plantlet and total number of plantlet than A2-A2R medium. We obtained 43 maintainer lines originated from anther culture of F1 (IR58025B x Sintanur and IR62829B x Ciherang) and 55 restorer lines originated from anther culture F1 (IR53942R x Ciherang and BR827-35R x Sintanur).},
    keywords = {Hybrid rice, male sterile, restorer, anther culture},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_226-235_iswari_perbaikan.pdf}
    }
  23. Sunarlim, N., I. Mariska, and R. Purnamaningsih. 2003. Inisiasi Akar Manggis dari Tunas In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 236-242.
    [BibTeX] [Abstract] [PDF: Inisiasi Akar Manggis dari Tunas In Vitro ]
    The growth of mangosteen shoots through in vitro culture was already succeeded, but the root formation is still in a problem. An experiment of root formation was conducted in the tissue culture laboratory of Indonesian Agricultural Biotechnology and Germplasm Resources Research Institute in the year of 2002. The experiment consisted of 2 base madia (WPM and MS) with 3 rates of base media (1, ½ and ¼ formulation) and application of IBA (5 and 10 mg/l). Result of the experiment showed that the medium of WPM was better than that of MS on the root formation. At 3 months old, explants in the media of WPM (WPM + IBA 5 mg/l, WPM + IBA 10 mg/l, ½ WPM + IBA 10 mg/l, ¼ WPM + IBA 5 mg/l, dan ¼ WPM + IBA 10 mg/l) were already rooted, but it needed 6 months for explants in MS media to form roots and only one treatment (¼ MS + IBA 5 mg/l) has the shoots rooted. The highest percentage of shoots rooted was found in the treatment of ¼ WPM + IBA 10 mg/l which was 66.7%.
    @article{Sunarlim03p236,
    title = {{Inisiasi Akar Manggis dari Tunas In Vitro}},
    author = {N. Sunarlim and I. Mariska and R. Purnamaningsih},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {236-242},
    year = {2003},
    abstract = {The growth of mangosteen shoots through in vitro culture was already succeeded, but the root formation is still in a problem. An experiment of root formation was conducted in the tissue culture laboratory of Indonesian Agricultural Biotechnology and Germplasm Resources Research Institute in the year of 2002. The experiment consisted of 2 base madia (WPM and MS) with 3 rates of base media (1, ½ and ¼ formulation) and application of IBA (5 and 10 mg/l). Result of the experiment showed that the medium of WPM was better than that of MS on the root formation. At 3 months old, explants in the media of WPM (WPM + IBA 5 mg/l, WPM + IBA 10 mg/l, ½ WPM + IBA 10 mg/l, ¼ WPM + IBA 5 mg/l, dan ¼ WPM + IBA 10 mg/l) were already rooted, but it needed 6 months for explants in MS media to form roots and only one treatment (¼ MS + IBA 5 mg/l) has the shoots rooted. The highest percentage of shoots rooted was found in the treatment of ¼ WPM + IBA 10 mg/l which was 66.7%.},
    keywords = {mangosteen, garcinia mangostana, growth, root formation},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_236-242_novianti_inisiasi.pdf}
    }
  24. Noviati, A. V., N. Sunarlim, Murtado, W. H. Adil, and Hadiatmi. 2003. Induksi dan Multiplikasi Tunas Gembili dan Kentang Hitam untuk Penyimpanan secara Kultur In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 243-249.
    [BibTeX] [Abstract] [PDF: Induksi dan Multiplikasi Tunas Gembili dan Kentang Hitam untuk Penyimpanan secara Kultur In Vitro ]
    Coleus tuberosus and Dioscorea aculeata are potential tuber crops in Indonesia. Preservation for these crops usually conducted by planting them in the collection field which are laborious, time consuming and need a large area. In vitro preservation is an alternative method to solve those problems. Before in vitro preservation is conducted, multiplication methods of Coleus tuberosus and Dioscorea aculeata should be studied. In this experiment, BAP (0, 0.5, 1.0, and 2.0 mg/l) and kinetin (0, 0.5, 1.0, 2.0, and 4.0 mg/l) were used to induce shoot formation. The use of BAP on the medium until certain concentration increased the number of shoot of C. tuberosus and D. aculeata. BAP concentration as much as 1.1 mg/l gave maximum result of the number of shoot. The use of kinetin in the medium gave no significant effect to the number of C. tuberosus and D. aculeata shoots.
    @article{Noviati03p243,
    title = {{Induksi dan Multiplikasi Tunas Gembili dan Kentang Hitam untuk Penyimpanan secara Kultur In Vitro}},
    author = {A. V. Noviati and N. Sunarlim and Murtado and W. H. Adil and Hadiatmi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {243-249},
    year = {2003},
    abstract = {Coleus tuberosus and Dioscorea aculeata are potential tuber crops in Indonesia. Preservation for these crops usually conducted by planting them in the collection field which are laborious, time consuming and need a large area. In vitro preservation is an alternative method to solve those problems. Before in vitro preservation is conducted, multiplication methods of Coleus tuberosus and Dioscorea aculeata should be studied. In this experiment, BAP (0, 0.5, 1.0, and 2.0 mg/l) and kinetin (0, 0.5, 1.0, 2.0, and 4.0 mg/l) were used to induce shoot formation. The use of BAP on the medium until certain concentration increased the number of shoot of C. tuberosus and D. aculeata. BAP concentration as much as 1.1 mg/l gave maximum result of the number of shoot. The use of kinetin in the medium gave no significant effect to the number of C. tuberosus and D. aculeata shoots.},
    keywords = {dioscorea aculeata, coleus tuberosus, in vitro culture, multiplication},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_243-249_vivi_induksi.pdf}
    }
  25. Supriati, Y., W. H. Adil, Y. Rusyadi, and I. Mariska. 2003. Optimasi Sistem Perakaran dan Aklimatisasi Iles-iles (Amorpophalus sp.). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 250-256.
    [BibTeX] [Abstract] [PDF: Optimasi Sistem Perakaran dan Aklimatisasi Iles-iles (Amorpophalus sp.) ]
    Iles-iles (Amorpophalus sp.) plant as a source of glucomanan which is highly in fiber content has a good potential as an commodity export. The main usage of iles-iles flour is for food industry which produce many kind of healthy foods, while the other usages are for industry raw material such as negatif film and glue. The main constraint for development of this plant in Indonesia was low production due to lack of information of iles-iles function and usage in the daily life. Iles-iles for export was collected from farmer who collects iles-iles the wild in forest or estate. On ROPP 2000 and ROPP 2001, the research had obtained planlet with good shoots and on ROPP 2002 they were used to induce optimized roots and aclimatized in the greenhouse. For inducing roots, the planlets were subculture in MS medium (½ and ¼) and combined with four level of IAA (0, 0.5, 1.0, and 1.5mg/l). The experiment used complete randomized design and repeated 10 times. Observation included number of roots, roots length, and visual performance. For aclimatization, five growth medium were used (soil, casting, manure, compost, and rice husk). The palnt were arranged in completely randomized block design and repeated 10 times. Observation was conducted on plant height, number of shoot, number of pseudotuber, and stem quality. This research showed that ¼ MS medium without growth regulator was good for inducing roots by in vitro culture and for aclimatization, the combination between soil and casting with ration 1:1 was the best medium.
    @article{Supriati03p250,
    title = {{Optimasi Sistem Perakaran dan Aklimatisasi Iles-iles (Amorpophalus sp.)}},
    author = {Y. Supriati and W. H. Adil and Y. Rusyadi and I. Mariska},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {250-256},
    year = {2003},
    abstract = {Iles-iles (Amorpophalus sp.) plant as a source of glucomanan which is highly in fiber content has a good potential as an commodity export. The main usage of iles-iles flour is for food industry which produce many kind of healthy foods, while the other usages are for industry raw material such as negatif film and glue. The main constraint for development of this plant in Indonesia was low production due to lack of information of iles-iles function and usage in the daily life. Iles-iles for export was collected from farmer who collects iles-iles the wild in forest or estate. On ROPP 2000 and ROPP 2001, the research had obtained planlet with good shoots and on ROPP 2002 they were used to induce optimized roots and aclimatized in the greenhouse. For inducing roots, the planlets were subculture in MS medium (½ and ¼) and combined with four level of IAA (0, 0.5, 1.0, and 1.5mg/l). The experiment used complete randomized design and repeated 10 times. Observation included number of roots, roots length, and visual performance. For aclimatization, five growth medium were used (soil, casting, manure, compost, and rice husk). The palnt were arranged in completely randomized block design and repeated 10 times. Observation was conducted on plant height, number of shoot, number of pseudotuber, and stem quality. This research showed that ¼ MS medium without growth regulator was good for inducing roots by in vitro culture and for aclimatization, the combination between soil and casting with ration 1:1 was the best medium.},
    keywords = {iles-iles, amorphopalus sp, roots system optimization, in vitro culture, aclimatization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_250-256_yatisupriyati_optimasi.pdf}
    }
  26. Lestari, Endang G. and Ika Mariska. 2003. Pengaruh berbagai Formulasi Media terhadap Regenerasi Kalus Padi Indica. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 257-263.
    [BibTeX] [Abstract] [PDF: Pengaruh berbagai Formulasi Media terhadap Regenerasi Kalus Padi Indica ]
    In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic factors including water stress resistant. To obtain the optimum result of variety improvement, regeneration system should firstly found out. It is sufficiently difficult to regenerate Indica rice. Hence, with this system, the improvement of Indica rice can produce tolerant character to water stress through in vitro selection with PEG (0-30%). Cisadane, Bengawan Solo, Towuti, Gajahmungkur, and Jatiluhur were Indica rice varieties using this experiment. Callus was produced from zygotic embryo which is cultured at medium MS + 2.4-D 2 mg/l + casein hidrolisat 2 g/l and regenerated at MS + BA 1-5 mg/l + IAA 0.1 mg/l or thidiazuron (0.2-0.4 mgl), zeatin 0.1 mg/l or prolin 100 mg/l. MS (1, ½) + IAA 0.1 mg/l was used for root development. Regeneration of Cisadane and Bengawan Solo were 70 and 60% respectively with subculture at the same medium (MS + BA 3 and 5 mg/l + IAA 0.1 mg/l + zeatin 0.1 mg/l).
    @article{Lestari03p257,
    title = {{Pengaruh berbagai Formulasi Media terhadap Regenerasi Kalus Padi Indica}},
    author = {Endang G. Lestari and Ika Mariska},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {257-263},
    year = {2003},
    abstract = {In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic factors including water stress resistant. To obtain the optimum result of variety improvement, regeneration system should firstly found out. It is sufficiently difficult to regenerate Indica rice. Hence, with this system, the improvement of Indica rice can produce tolerant character to water stress through in vitro selection with PEG (0-30%). Cisadane, Bengawan Solo, Towuti, Gajahmungkur, and Jatiluhur were Indica rice varieties using this experiment. Callus was produced from zygotic embryo which is cultured at medium MS + 2.4-D 2 mg/l + casein hidrolisat 2 g/l and regenerated at MS + BA 1-5 mg/l + IAA 0.1 mg/l or thidiazuron (0.2-0.4 mgl), zeatin 0.1 mg/l or prolin 100 mg/l. MS (1, ½) + IAA 0.1 mg/l was used for root development. Regeneration of Cisadane and Bengawan Solo were 70 and 60% respectively with subculture at the same medium (MS + BA 3 and 5 mg/l + IAA 0.1 mg/l + zeatin 0.1 mg/l).},
    keywords = {in vitro culture, indica rice and regeneration},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_257-263_endanggati_pengaruh.pdf}
    }
  27. Purnamaningsih, Ragapadmi and Ika Mariska. 2003. Regenerasi Kalus Embrionik Padi setelah Diseleksi dengan Al dan pH Rendah. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 264-271.
    [BibTeX] [Abstract] [PDF: Regenerasi Kalus Embrionik Padi setelah Diseleksi dengan Al dan pH Rendah ]
    Using of acid land to increase national production of rice is another alternative. The main problem of the land is abiotic stress: high acidity, low availability of N, P, K, Ca, Mg, Mo and high concentration of Al and Mn. Improvement cultivars to abiotic stress. Can solve the problem. The objective of this research is to conduct selection method and new rice varieties tolerance to acid soil (low pH and high concentration of Al). The treatment was varieties (T-309, Rojolele, and IR64), dosage of radiation (0, 100, 300, and 500 rad) and aluminum concentra-tion (0, 100, 200, 300, 400, and 500 ppm). Selection done at 2 stages: callus stage and regeneration stage. Randomized Complete Design was used in this experiment. The results showed that increasing of dosage radiation could decrease callus regeneration of rice. Embrionic cell masses which selection with Al and low pH could regenerate to form shoot with different regeneration ability between varieties depend on concentration aluminum. Increase of Al concentra-tion could decrease callus regeneration ability to form shoot. Selection on regeneration stage given higher percentage of regeneration than callus stage.
    @article{RagapadmiPurnamaningsih03p264,
    title = {{Regenerasi Kalus Embrionik Padi setelah Diseleksi dengan Al dan pH Rendah}},
    author = {Ragapadmi Purnamaningsih and Ika Mariska},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {264-271},
    year = {2003},
    abstract = {Using of acid land to increase national production of rice is another alternative. The main problem of the land is abiotic stress: high acidity, low availability of N, P, K, Ca, Mg, Mo and high concentration of Al and Mn. Improvement cultivars to abiotic stress. Can solve the problem. The objective of this research is to conduct selection method and new rice varieties tolerance to acid soil (low pH and high concentration of Al). The treatment was varieties (T-309, Rojolele, and IR64), dosage of radiation (0, 100, 300, and 500 rad) and aluminum concentra-tion (0, 100, 200, 300, 400, and 500 ppm). Selection done at 2 stages: callus stage and regeneration stage. Randomized Complete Design was used in this experiment. The results showed that increasing of dosage radiation could decrease callus regeneration of rice. Embrionic cell masses which selection with Al and low pH could regenerate to form shoot with different regeneration ability between varieties depend on concentration aluminum. Increase of Al concentra-tion could decrease callus regeneration ability to form shoot. Selection on regeneration stage given higher percentage of regeneration than callus stage.},
    keywords = {rice, in vitro selection, al, ph},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_264-271_ragapadmi_regenerasi.pdf}
    }
  28. Husni, A., S. Hutami, M. Kosmiatin, and I. Mariska. 2003. Regenerasi Massa Sel Embriogenik Kedelai yang Diseleksi dengan Polyethylen Glicol 6000 (PEG). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 272-280.
    [BibTeX] [Abstract] [PDF: Regenerasi Massa Sel Embriogenik Kedelai yang Diseleksi dengan Polyethylen Glicol 6000 (PEG) ]
    Improvement of soybean variety which tolerant to marginal as drought, can increase productivity. In vitro culture is one alternative technology to improve variety by selection of embryogenic cell mass. Using the technology produced plant which tolerant to biotic and abiotic stress. In this experiment explant of embryo zygotic was radiated by gamma rays (400 rad) and embriogenic cell mass were selected to water stress by PEG 6000 (0-75%). The aim of this experiment was producing embryo somatic structure of soybean, which adapted to PEG solution. Embryogenic callus were produced from three soybean varieties (Sindoro, Wilis, and Slamet) in M4C medium. The percentage of callus formation high was Wilis (96.5%). The rate of embryo structure production was 4.13 from Sindoro, 3.54 from Wilis, and 2.84 from Slamet varieties. PEG addition at the medium caused the death of culture. Increasing PEG concen-tration caused increasing the death of embryo somatic structure. The number of embryo somatic structure which tolerant to PEG 25% were 18 for Sindoro, 42 for Wilis, and 36 for Slamet varieties. Number of embryo somatic structure at PEG 50% were 9 for Sindoro, 20 for Wilis, and 19 for Slamet. Total number of embryo somatic structure, which adaptive to PEG solution were 27 for Sindoro, 62 for Wilis, and 55 for Slamet. The embryo somatic structure were grown for seedling right now to produce soybean somatic seed which adaptive to PEG.
    @article{Husni03p272,
    title = {{Regenerasi Massa Sel Embriogenik Kedelai yang Diseleksi dengan Polyethylen Glicol 6000 (PEG)}},
    author = {A. Husni and S. Hutami and M. Kosmiatin and I. Mariska},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {272-280},
    year = {2003},
    abstract = {Improvement of soybean variety which tolerant to marginal as drought, can increase productivity. In vitro culture is one alternative technology to improve variety by selection of embryogenic cell mass. Using the technology produced plant which tolerant to biotic and abiotic stress. In this experiment explant of embryo zygotic was radiated by gamma rays (400 rad) and embriogenic cell mass were selected to water stress by PEG 6000 (0-75%). The aim of this experiment was producing embryo somatic structure of soybean, which adapted to PEG solution. Embryogenic callus were produced from three soybean varieties (Sindoro, Wilis, and Slamet) in M4C medium. The percentage of callus formation high was Wilis (96.5%). The rate of embryo structure production was 4.13 from Sindoro, 3.54 from Wilis, and 2.84 from Slamet varieties. PEG addition at the medium caused the death of culture. Increasing PEG concen-tration caused increasing the death of embryo somatic structure. The number of embryo somatic structure which tolerant to PEG 25% were 18 for Sindoro, 42 for Wilis, and 36 for Slamet varieties. Number of embryo somatic structure at PEG 50% were 9 for Sindoro, 20 for Wilis, and 19 for Slamet. Total number of embryo somatic structure, which adaptive to PEG solution were 27 for Sindoro, 62 for Wilis, and 55 for Slamet. The embryo somatic structure were grown for seedling right now to produce soybean somatic seed which adaptive to PEG.},
    keywords = {soybean, in vitro selection, dry land, peg 6000},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_272-280_alihusni_regenerasi.pdf}
    }
  29. Hutami, S., M. Kosmiatin, I. Mariska, and A. Husni. 2003. Penyelamatan Embrio Hasil Persilangan Kacang Hijau dengan Kerabat Liarnya. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 281-293.
    [BibTeX] [Abstract] [PDF: Penyelamatan Embrio Hasil Persilangan Kacang Hijau dengan Kerabat Liarnya ]
    Mungbean (Vigna radiata L. Wilczek) have good prospect to develop in dry land. Scab is ones of 7 kinds important diseases of mungbean plant caused by Elsinoe iwatae. The disease attack in wet season caused decreasing yield up to 60%. Until now there was no mungbean variety tolerant to Scab. The tolerant character found in the wild species (Vigna mungo L. Hepper). There was incompatibility in crossing between mungbean and the wild species (V. radiata x V. mungo). It caused abnormal seed, low germination precentage, and F1 sterile. Embryo rescue at early stage and multiply chromosome at F1 generation is one of the methods to solve the problem. In vitro culture used to rescue the embryo or seed from crossing by cultured in growth medium. In this experiment 4 mungbean varieties (Walet as V. radiata and VR-34, VR-45, No. 19/1 as V. mungo were planted in green house. Complete Randomized Design with Factorial was used in this experiment. The treatment were two factors (A) age of young seed (1, 2, and 3 weeks after pollination) and (B) formulation medium (1) MS + kinetin 0.2 mg/l + IAA 1 mg/l + casein hidrolisat 0.5 g/l; (2) Knudson; (3) Knudson C; (4) Knudson + BA 1 mg/l; (5) Knudson C + BA 1 mg/l. Cross-pollination was done between V. radiata and V. mungo with their reciprocal. After pollination the young seeds were cultured on treatment medium. Observation was done on percentage of young embryo/seed seedling, number of bud and visually observation of culture. The result showed that the highest percentage of cross- pollination was crossing between Walet and No. 19/1. Seedling of embryo from cross-pollination with Walet as female parent increased with increasing age of embryo. Embryo developed at all treatment medium and increased with addition of BA 1 mg/l. Embryo with Vigna mungo as female parent could not develop until the end of the experiment.
    @article{Hutami03p281,
    title = {{Penyelamatan Embrio Hasil Persilangan Kacang Hijau dengan Kerabat Liarnya}},
    author = {S. Hutami and M. Kosmiatin and I. Mariska and A. Husni},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {281-293},
    year = {2003},
    abstract = { Mungbean (Vigna radiata L. Wilczek) have good prospect to develop in dry land. Scab is ones of 7 kinds important diseases of mungbean plant caused by Elsinoe iwatae. The disease attack in wet season caused decreasing yield up to 60%. Until now there was no mungbean variety tolerant to Scab. The tolerant character found in the wild species (Vigna mungo L. Hepper). There was incompatibility in crossing between mungbean and the wild species (V. radiata x V. mungo). It caused abnormal seed, low germination precentage, and F1 sterile. Embryo rescue at early stage and multiply chromosome at F1 generation is one of the methods to solve the problem. In vitro culture used to rescue the embryo or seed from crossing by cultured in growth medium. In this experiment 4 mungbean varieties (Walet as V. radiata and VR-34, VR-45, No. 19/1 as V. mungo were planted in green house. Complete Randomized Design with Factorial was used in this experiment. The treatment were two factors (A) age of young seed (1, 2, and 3 weeks after pollination) and (B) formulation medium (1) MS + kinetin 0.2 mg/l + IAA 1 mg/l + casein hidrolisat 0.5 g/l; (2) Knudson; (3) Knudson C; (4) Knudson + BA 1 mg/l; (5) Knudson C + BA 1 mg/l. Cross-pollination was done between V. radiata and V. mungo with their reciprocal. After pollination the young seeds were cultured on treatment medium. Observation was done on percentage of young embryo/seed seedling, number of bud and visually observation of culture. The result showed that the highest percentage of cross- pollination was crossing between Walet and No. 19/1. Seedling of embryo from cross-pollination with Walet as female parent increased with increasing age of embryo. Embryo developed at all treatment medium and increased with addition of BA 1 mg/l. Embryo with Vigna mungo as female parent could not develop until the end of the experiment.},
    keywords = {Mungbean, embryo rescue, cross-pollination},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_281-293_srihutami_penyelamatan.pdf}
    }
  30. Tambunan, I. R., N. Sunarlim, I. Mariska, and M. Kosmiatin. 2003. Pengaruh Beberapa Krioprotektan terhadap Keberhasilan Penyimpanan Ubi-ubian secara Kriopreservasi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 294-301.
    [BibTeX] [Abstract] [PDF: Pengaruh Beberapa Krioprotektan terhadap Keberhasilan Penyimpanan Ubi-ubian secara Kriopreservasi ]
    Cryopreservation is potential in vitro method for long term preservation of germplasm. The research was conducted in 2002 and divided into two experiments. The first experiment was cryopreservation of cassava (Manihot utilissima) to obtain optimum preculture duration and to study the effect of several cryoprotectants in several loading times. The second experiments was cryopreservation of yam (Dioscorea alata) to study the effect of several preculture mediums and to obtain the optimal preculture duration. In the cassava cryopreservation experiment, preculture was conducted in the MS medium containing 0.3 M of sucrose for 1, 2, and 4 days of preculture duration. Loading was conducted for 10, 20, and 30 minutes. Cryoprotectant solutions that be used were PVS1, PVS2, and PVS3. In the yam cryopreservation experiment, preculture was conducted in MS medium containing 100 ppm of citric acid and supplemented with sucrose at level 0.3, 0.5, and 0.7 M. Result showed that the optimum preculture duration of cassava cryopreservation was 4 days. The combination of 4 days of preculture and 20 minutes of loading showed the best result for all kind of cryoprotectants that be used. The PVS3 (50% gliserol + 50% sucrose in MS media) gave the lowest inhibitation of cultures growth. The yam cryopreservation experiment showed that yam culture had high tolerance to high level of sucrose to 0.7 M in the preculture treatment.
    @article{Tambunan03p294,
    title = {{Pengaruh Beberapa Krioprotektan terhadap Keberhasilan Penyimpanan Ubi-ubian secara Kriopreservasi}},
    author = {I. R. Tambunan and N. Sunarlim and I. Mariska and M. Kosmiatin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {294-301},
    year = {2003},
    abstract = {Cryopreservation is potential in vitro method for long term preservation of germplasm. The research was conducted in 2002 and divided into two experiments. The first experiment was cryopreservation of cassava (Manihot utilissima) to obtain optimum preculture duration and to study the effect of several cryoprotectants in several loading times. The second experiments was cryopreservation of yam (Dioscorea alata) to study the effect of several preculture mediums and to obtain the optimal preculture duration. In the cassava cryopreservation experiment, preculture was conducted in the MS medium containing 0.3 M of sucrose for 1, 2, and 4 days of preculture duration. Loading was conducted for 10, 20, and 30 minutes. Cryoprotectant solutions that be used were PVS1, PVS2, and PVS3. In the yam cryopreservation experiment, preculture was conducted in MS medium containing 100 ppm of citric acid and supplemented with sucrose at level 0.3, 0.5, and 0.7 M. Result showed that the optimum preculture duration of cassava cryopreservation was 4 days. The combination of 4 days of preculture and 20 minutes of loading showed the best result for all kind of cryoprotectants that be used. The PVS3 (50% gliserol + 50% sucrose in MS media) gave the lowest inhibitation of cultures growth. The yam cryopreservation experiment showed that yam culture had high tolerance to high level of sucrose to 0.7 M in the preculture treatment.},
    keywords = {cryopreservation, manihot utilissima, dioscorea alata},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_294-301_ikaroostika_pengaruh.pdf}
    }
  31. Sukmadjaja, D., I. Mariska, E. G. Lestari, M. Tombe, and M. Kosmiatin. 2003. Pengujian Planlet Abaka Hasil Seleksi terhadap Fusarium oxysporum. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 30-45.
    [BibTeX] [Abstract] [PDF: Pengujian Planlet Abaka Hasil Seleksi terhadap Fusarium oxysporum ]
    Cryopreservation is potential in vitro method for long term preservation of germplasm. The research was conducted in 2002 and divided into two experiments. The first experiment was cryopreservation of cassava (Manihot utilissima) to obtain optimum preculture duration and to study the effect of several cryoprotectants in several loading times. The second experiments was cryopreservation of yam (Dioscorea alata) to study the effect of several preculture mediums and to obtain the optimal preculture duration. In the cassava cryopreservation experiment, preculture was conducted in the MS medium containing 0.3 M of sucrose for 1, 2, and 4 days of preculture duration. Loading was conducted for 10, 20, and 30 minutes. Cryoprotectant solutions that be used were PVS1, PVS2, and PVS3. In the yam cryopreservation experiment, preculture was conducted in MS medium containing 100 ppm of citric acid and supplemented with sucrose at level 0.3, 0.5, and 0.7 M. Result showed that the optimum preculture duration of cassava cryopreservation was 4 days. The combination of 4 days of preculture and 20 minutes of loading showed the best result for all kind of cryoprotectants that be used. The PVS3 (50% gliserol + 50% sucrose in MS media) gave the lowest inhibitation of cultures growth. The yam cryopreservation experiment showed that yam culture had high tolerance to high level of sucrose to 0.7 M in the preculture treatment.
    @article{Sukmadjaja03p30,
    title = {{Pengujian Planlet Abaka Hasil Seleksi terhadap Fusarium oxysporum}},
    author = {D. Sukmadjaja and I. Mariska and E. G. Lestari and M. Tombe and M. Kosmiatin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {30 - 45},
    year = {2003},
    abstract = { Cryopreservation is potential in vitro method for long term preservation of germplasm. The research was conducted in 2002 and divided into two experiments. The first experiment was cryopreservation of cassava (Manihot utilissima) to obtain optimum preculture duration and to study the effect of several cryoprotectants in several loading times. The second experiments was cryopreservation of yam (Dioscorea alata) to study the effect of several preculture mediums and to obtain the optimal preculture duration. In the cassava cryopreservation experiment, preculture was conducted in the MS medium containing 0.3 M of sucrose for 1, 2, and 4 days of preculture duration. Loading was conducted for 10, 20, and 30 minutes. Cryoprotectant solutions that be used were PVS1, PVS2, and PVS3. In the yam cryopreservation experiment, preculture was conducted in MS medium containing 100 ppm of citric acid and supplemented with sucrose at level 0.3, 0.5, and 0.7 M. Result showed that the optimum preculture duration of cassava cryopreservation was 4 days. The combination of 4 days of preculture and 20 minutes of loading showed the best result for all kind of cryoprotectants that be used. The PVS3 (50% gliserol + 50% sucrose in MS media) gave the lowest inhibitation of cultures growth. The yam cryopreservation experiment showed that yam culture had high tolerance to high level of sucrose to 0.7 M in the preculture treatment.},
    keywords = {Cryopreservation, Manihot utilissima, Dioscorea alata},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_302-308_deden_pengujian.pdf}
    }
  32. Bahagiawati, Habib Rijzaani, M. Iman, Harnoto, Tri P. Priyatno, and Haeni Purwanti. 2003. Konfirmasi Virulensi Beberapa Isolat Bt Lokal yang Mengandung Gen Cry terhadap Hama Tanaman. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 309-317.
    [BibTeX] [Abstract] [PDF: Konfirmasi Virulensi Beberapa Isolat Bt Lokal yang Mengandung Gen Cry terhadap Hama Tanaman ]
    The toxicity of several Bt isolates were tested against 4 different plant pests i.e. Ostrinia furnacalis, Spodoptera lituralis, Plutella xylostella, and Phaedonia inclusa. For the first two pests, the artificial diet was used for bioassays while for the last two was leaf disk bioassays. The result of this experiment showed that from 15 isolates tested against O. furnacalis, only 6 isolat showed larval mortality over 80% while against S. lituralis from 6 isolates tested, only 3 isolates showed larval mortality over 50%. Twelve isolates were tested against P. xylostella and only 6 isolates gave mortality over 90%. For P. inclusa from 13 isolates tested, 4 isolates showed relatively hight toxicity with LD50 between 2.0-3.3 days.
    @article{Bahagiawati03p309,
    title = {{Konfirmasi Virulensi Beberapa Isolat Bt Lokal yang Mengandung Gen Cry terhadap Hama Tanaman}},
    author = {Bahagiawati and Habib Rijzaani and M. Iman and Harnoto and Tri P. Priyatno and Haeni Purwanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {309-317},
    year = {2003},
    abstract = {The toxicity of several Bt isolates were tested against 4 different plant pests i.e. Ostrinia furnacalis, Spodoptera lituralis, Plutella xylostella, and Phaedonia inclusa. For the first two pests, the artificial diet was used for bioassays while for the last two was leaf disk bioassays. The result of this experiment showed that from 15 isolates tested against O. furnacalis, only 6 isolat showed larval mortality over 80% while against S. lituralis from 6 isolates tested, only 3 isolates showed larval mortality over 50%. Twelve isolates were tested against P. xylostella and only 6 isolates gave mortality over 90%. For P. inclusa from 13 isolates tested, 4 isolates showed relatively hight toxicity with LD50 between 2.0-3.3 days.},
    keywords = {bacillus thuringiensis, cry gene, pest},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_309-317_bahagiawati_konfirmasi.pdf}
    }
  33. Rijzaani, Habib and Bahagiawati. 2003. Ekstraksi DNA Bacillus thuringiensis Isolat Lokal yang Mengandung Gen Cry1 untuk Pembuatan Pustaka Plasmid Bt. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 318-327.
    [BibTeX] [Abstract] [PDF: Ekstraksi DNA Bacillus thuringiensis Isolat Lokal yang Mengandung Gen Cry1 untuk Pembuatan Pustaka Plasmid Bt ]
    An effort to clone cry1 gene from local isolates of Bacillus thuringiensis had been initiated by screening using PCR. Eleven local isolates of B. thuringiensis were identified possesing cry1 gene based on the amplification of plasmid DNA using primers specific for cry1A genes. Only five from these eleven isolates produced one ® 490 bp band like the positive control, Dipel . DNA plasmid that contains this gene sequence, from chosen isolate Jtg2151, had been isolated and digested with restriction enzymes. Based on the size of the DNA fragments produced, restriction enzymes EcoRI, HindIII, and PstI will be used to generate plasmid library of B. thuringiensis.
    @article{HabibRijzaani03p318,
    title = {{Ekstraksi DNA Bacillus thuringiensis Isolat Lokal yang Mengandung Gen Cry1 untuk Pembuatan Pustaka Plasmid Bt}},
    author = {Habib Rijzaani and Bahagiawati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {318-327},
    year = {2003},
    abstract = {An effort to clone cry1 gene from local isolates of Bacillus thuringiensis had been initiated by screening using PCR. Eleven local isolates of B. thuringiensis were identified possesing cry1 gene based on the amplification of plasmid DNA using primers specific for cry1A genes. Only five from these eleven isolates produced one ® 490 bp band like the positive control, Dipel . DNA plasmid that contains this gene sequence, from chosen isolate Jtg2151, had been isolated and digested with restriction enzymes. Based on the size of the DNA fragments produced, restriction enzymes EcoRI, HindIII, and PstI will be used to generate plasmid library of B. thuringiensis.},
    keywords = {cry1a gene, bacillus thuringiensis, cloning},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_318-327_habib_ekstraksi.pdf}
    }
  34. Manzila, I., M. Machmud, Jumanto, and Y. Suryadi. 2003. Evaluasi Lapangan Perangkat ELISA dengan Antibodi Poliklonal untuk Deteksi dan Identifikasi RRSV dan Ralstonia solanacearum. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 328-339.
    [BibTeX] [Abstract] [PDF: Evaluasi Lapangan Perangkat ELISA dengan Antibodi Poliklonal untuk Deteksi dan Identifikasi RRSV dan Ralstonia solanacearum ]
    A laboratory trial was conducted with objectives to evaluate effectiveness of ELISA kits for detection and identification of RRSV and RS. ELISA kits for each of the pathogen was prepared in a separate package. Each package contained antigen, antibody, conjugate, buffers, substrate, and ELISA microplates and applicable for detection of 250 plant samples. DAS-ELISA technique was used for the detection of RRSV, while Indirect ELISA technique was used for the detection of RS. Rice plant showing ragged stunt symptoms were collected from Muara and Sukamandi Experimental Fields and used as plant samples for the detection of RRSV. Potato tubers infected with RS that were collected from farmers’ fields in Pangalengan and Lembang were used as plant samples for the detection of RS. Leaves of rice plants infected with RRSV that were maintained in a glasshouse were used as positive control for RRSV. Bacterial culture of RS isolate number RS 9001 were used as control positive for RS. Results of the trial showed that the ELISA kits were effective for the detection of both RRSV and RS. 4 The kits had sensitivity detections or detection limits of up to 0.5µg RRSV/ml and 10 RS sel/ml. The kits are ready for mass production.
    @article{Manzila03p328,
    title = {{Evaluasi Lapangan Perangkat ELISA dengan Antibodi Poliklonal untuk Deteksi dan Identifikasi RRSV dan Ralstonia solanacearum}},
    author = {I. Manzila and M. Machmud and Jumanto and Y. Suryadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {328-339},
    year = {2003},
    abstract = {A laboratory trial was conducted with objectives to evaluate effectiveness of ELISA kits for detection and identification of RRSV and RS. ELISA kits for each of the pathogen was prepared in a separate package. Each package contained antigen, antibody, conjugate, buffers, substrate, and ELISA microplates and applicable for detection of 250 plant samples. DAS-ELISA technique was used for the detection of RRSV, while Indirect ELISA technique was used for the detection of RS. Rice plant showing ragged stunt symptoms were collected from Muara and Sukamandi Experimental Fields and used as plant samples for the detection of RRSV. Potato tubers infected with RS that were collected from farmers' fields in Pangalengan and Lembang were used as plant samples for the detection of RS. Leaves of rice plants infected with RRSV that were maintained in a glasshouse were used as positive control for RRSV. Bacterial culture of RS isolate number RS 9001 were used as control positive for RS. Results of the trial showed that the ELISA kits were effective for the detection of both RRSV and RS. 4 The kits had sensitivity detections or detection limits of up to 0.5µg RRSV/ml and 10 RS sel/ml. The kits are ready for mass production.},
    keywords = {elisa kits using polyclonal antibody, detection and indetifation, rrsv and rs},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_328-339_ifa_evaluasi.pdf}
    }
  35. Jumanto, M. Machmud, Ifa Manzila, and Yadi Suryadi. 2003. Produksi Antibodi Monoklonal (McAb) untuk Deteksi Virus Kerdil Hampa Padi: Produksi Hibridoma Penghasil McAb. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 340-349.
    [BibTeX] [Abstract] [PDF: Produksi Antibodi Monoklonal (McAb) untuk Deteksi Virus Kerdil Hampa Padi: Produksi Hibridoma Penghasil McAb ]
    A technique for production of monoclonal antibody was adopted for the production of McAb RRSV. Mice hybrid Balb c was immunized by injecting with RRSV antigen. Limphocytes were harvested from spleen of the immunized mice. Cell fusion was done by mixing the lymphocytes with SP2/O-Ag14 myeloma cells. The hybridomas, results of the cell fusion, were selected for those producing McAb. Results of the trial showed that hybridomas producing McAb RRSV were obtained from the cell fusion. Among them, two hybridoma colonies, RR-(2)-3 C10 and RR-(3)-2 H03, were considered potential as sources for McAb RRSV productions. These colonies were stored under cryogenic condition in a liquid nitrogen tank. The colonies were ready for cloning to obtain single-cell colony hybridomas producing high titer and specific McAb RRSV.
    @article{Jumanto03p340,
    title = {{Produksi Antibodi Monoklonal (McAb) untuk Deteksi Virus Kerdil Hampa Padi: Produksi Hibridoma Penghasil McAb}},
    author = {Jumanto and M. Machmud and Ifa Manzila and Yadi Suryadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {340-349},
    year = {2003},
    abstract = {A technique for production of monoclonal antibody was adopted for the production of McAb RRSV. Mice hybrid Balb c was immunized by injecting with RRSV antigen. Limphocytes were harvested from spleen of the immunized mice. Cell fusion was done by mixing the lymphocytes with SP2/O-Ag14 myeloma cells. The hybridomas, results of the cell fusion, were selected for those producing McAb. Results of the trial showed that hybridomas producing McAb RRSV were obtained from the cell fusion. Among them, two hybridoma colonies, RR-(2)-3 C10 and RR-(3)-2 H03, were considered potential as sources for McAb RRSV productions. These colonies were stored under cryogenic condition in a liquid nitrogen tank. The colonies were ready for cloning to obtain single-cell colony hybridomas producing high titer and specific McAb RRSV.},
    keywords = {monoclonal antibody, detection, rice ragged stunt virus, rrsv},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_340-349_jumanto_produksi.pdf}
    }
  36. Machmud, M., Y. Suryadi, Jumanto, and I. Manzila. 2003. Teknik Produksi Antibodi Monoklonal (McAb) untuk Deteksi dan Identifikasi Ralstonia solanacearum. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 350-359.
    [BibTeX] [Abstract] [PDF: Teknik Produksi Antibodi Monoklonal (McAb) untuk Deteksi dan Identifikasi Ralstonia solanacearum ]
    A laboratory trial was done to adopt a technique for the production of monoclonal antibody (McAb) for detection of of bacterial wilt pathogen (Ralstonoa solanacearum, RS). Immbunization of mice hybrid Balb/c with RS antigen was successfully done. Limphoccytes from immunized mice were harvested and used for cell fusion with SP2/O-Ag14 myeloma cells to produce hybridomas. Hybridomas capable of producing McAb RS were selected from 300 hybridoma colonies. Three hybridoma colonies potentially capable of producing high titer of McAb RS were obtained, i.e., RS-(1)-1 C10, RS-(1)-1 F09, and RS (6)-2 F09. These hybridomas were stored under a cryogenic condition in a liquid nitrogen for further works.
    @article{Machmud03p350,
    title = {{Teknik Produksi Antibodi Monoklonal (McAb) untuk Deteksi dan Identifikasi Ralstonia solanacearum}},
    author = {M. Machmud and Y. Suryadi and Jumanto and I. Manzila},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {350-359},
    year = {2003},
    abstract = {A laboratory trial was done to adopt a technique for the production of monoclonal antibody (McAb) for detection of of bacterial wilt pathogen (Ralstonoa solanacearum, RS). Immbunization of mice hybrid Balb/c with RS antigen was successfully done. Limphoccytes from immunized mice were harvested and used for cell fusion with SP2/O-Ag14 myeloma cells to produce hybridomas. Hybridomas capable of producing McAb RS were selected from 300 hybridoma colonies. Three hybridoma colonies potentially capable of producing high titer of McAb RS were obtained, i.e., RS-(1)-1 C10, RS-(1)-1 F09, and RS (6)-2 F09. These hybridomas were stored under a cryogenic condition in a liquid nitrogen for further works.},
    keywords = {monoclonal antibody, detection and identification, elisa technique, ralstonia solanacearum},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_350-359_machmud_teknik.pdf}
    }
  37. Widowati, Sri and Misgiyarta. 2003. Efektifitas Bakteri Asam Laktat (BAL) dalam Pembuatan Produk Fermentasi Berbasis Protein/Susu Nabati. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 360-373.
    [BibTeX] [Abstract] [PDF: Efektifitas Bakteri Asam Laktat (BAL) dalam Pembuatan Produk Fermentasi Berbasis Protein/Susu Nabati ]
    In previous research we isolated five excellences Lactic Acid Bacteria (LAB) isolates. These isolates are F3, Nb3, KBB3, NNb PR5, and SLB 22. The fermentation effectivity of these isolates in legume milk media wasn’t observed. The aims of this research were (1) to observe of the fermentation effectivity in legume milk media and (2) to observe panelist preferency to fermented beans milk product. Parameter of fermentation effectivity in beans milk media is lactic acid concentration in media fermentation. The panelist preferency observed by organoleptic testing to fermented beans milk. The most effective couple of LAB isolates, beans milk media, and lactic acid concentration (%) showed respect-ively: mung bean milk-Nb3 = 1.55, kidney bean milk-KBB3 = 1.05, peanut milk-NNB PR5 = 0.85, legume milk-SLB 22 = 1.55, soybean milk-F3 = 0.95. Duncan’s test show that the panelist preferency to fermented kidney bean milk, fermented mung bean milk, and fermented legume milk compared to commer-cialed yoghurt are not significant. The panelist preferency to fermented peanut milk and fermented soybean milk compared to commercialed yoghurt are significant. The most preference of fermented beans milk by panelists is fermented kidney bean milk.
    @article{SriWidowati03p360,
    title = {{Efektifitas Bakteri Asam Laktat (BAL) dalam Pembuatan Produk Fermentasi Berbasis Protein/Susu Nabati}},
    author = {Sri Widowati and Misgiyarta},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {360-373},
    year = {2003},
    abstract = {In previous research we isolated five excellences Lactic Acid Bacteria (LAB) isolates. These isolates are F3, Nb3, KBB3, NNb PR5, and SLB 22. The fermentation effectivity of these isolates in legume milk media wasn't observed. The aims of this research were (1) to observe of the fermentation effectivity in legume milk media and (2) to observe panelist preferency to fermented beans milk product. Parameter of fermentation effectivity in beans milk media is lactic acid concentration in media fermentation. The panelist preferency observed by organoleptic testing to fermented beans milk. The most effective couple of LAB isolates, beans milk media, and lactic acid concentration (%) showed respect-ively: mung bean milk-Nb3 = 1.55, kidney bean milk-KBB3 = 1.05, peanut milk-NNB PR5 = 0.85, legume milk-SLB 22 = 1.55, soybean milk-F3 = 0.95. Duncan's test show that the panelist preferency to fermented kidney bean milk, fermented mung bean milk, and fermented legume milk compared to commer-cialed yoghurt are not significant. The panelist preferency to fermented peanut milk and fermented soybean milk compared to commercialed yoghurt are significant. The most preference of fermented beans milk by panelists is fermented kidney bean milk.},
    keywords = {lactic acid bacteria, effectivity, fermentation, legume milk, organoleptic test},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_360-373_sriwidowati_efektivitas.pdf}
    }
  38. Misgiyarta and Sri Widowati. 2003. Seleksi dan Karakterisasi Bakteri Asam Laktat (BAL) Indigenus. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 374-387.
    [BibTeX] [Abstract] [PDF: Seleksi dan Karakterisasi Bakteri Asam Laktat (BAL) Indigenus ]
    Lactic acid bacteria (LAB) grow well in Indonesia. Only a few laboratories have LAB culture collection. It’s very important enhace of LAB collection. The aims of this research are (1) to isolate of LAB from many sources and (2) to identificate of LAB. LAB isolation use selective media de Man Rogosa Sharpe Agar (MRS Agar). LAB was isolated from decaied cabbage, decaied mustard green, mustard green pickle, decaied long nourishing, decaied cabbage lettuce, decaied tomato, sauerkraut, mustard green contaminated milk, soy milk, decaied banana, decaied papaya, decaied pineapple, and decaied sour-sop. Isolates were identificated with test of gram, endospore coloring, catalase, motility, and ability to produce of lactic acid. We isolated 141 isolates of LAB. 31 isolates of 141 isolates are excellent to produce of lactic acid.
    @article{Misgiyarta03p374,
    title = {{Seleksi dan Karakterisasi Bakteri Asam Laktat (BAL) Indigenus}},
    author = {Misgiyarta and Sri Widowati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {374-387},
    year = {2003},
    abstract = {Lactic acid bacteria (LAB) grow well in Indonesia. Only a few laboratories have LAB culture collection. It's very important enhace of LAB collection. The aims of this research are (1) to isolate of LAB from many sources and (2) to identificate of LAB. LAB isolation use selective media de Man Rogosa Sharpe Agar (MRS Agar). LAB was isolated from decaied cabbage, decaied mustard green, mustard green pickle, decaied long nourishing, decaied cabbage lettuce, decaied tomato, sauerkraut, mustard green contaminated milk, soy milk, decaied banana, decaied papaya, decaied pineapple, and decaied sour-sop. Isolates were identificated with test of gram, endospore coloring, catalase, motility, and ability to produce of lactic acid. We isolated 141 isolates of LAB. 31 isolates of 141 isolates are excellent to produce of lactic acid.},
    keywords = {lactic acid bacteria, indigenous, isolation, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_374-387_misgiyarta_seleksi.pdf}
    }
  39. Richana, Nur and Pia Lestina. 2003. Produksi Xilanase untuk Biokonversi Limbah Biji Kedelai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 388-397.
    [BibTeX] [Abstract] [PDF: Produksi Xilanase untuk Biokonversi Limbah Biji Kedelai ]
    Production of xylanase for bioconvertion of waste from soybean grain. This experiment was carried out in Laboratory of Biochemistry and Enzymatic. The objectives of this research were obtain apropriate formula of medium consist xylane from outer skin of soybean grain and optimum condition of xylanase production. Medium formulate by 36 combinations were made based on variation of ingredients i.e. xylane, polypeptone, and yeast extract. Optimum condition of processing was determined based on combination of shaker speed, temperature, and pH, which produced of highest value of enzyme activity and specific enzyme. Extraction of xylane resulted 32 grams xylane from 1,000 grams outer skin of soybean grain.The best formula of medium for xylanase production using bacteria isolate AIII-5 from outer skin of soybean grain is combination of 0.5% polypeptone, 0.1% of yeast extract, and 1% xylane. The optimum o condition for processing is combination of 120 rpm, 35 C temperature, and pH 9, which resulted the highest value of enzyme activity 366.67 U/ml and enzyme specific activity 612.14 U/mg protein.
    @article{NurRichana03p388,
    title = {{Produksi Xilanase untuk Biokonversi Limbah Biji Kedelai}},
    author = {Nur Richana and Pia Lestina},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {388-397},
    year = {2003},
    abstract = {Production of xylanase for bioconvertion of waste from soybean grain. This experiment was carried out in Laboratory of Biochemistry and Enzymatic. The objectives of this research were obtain apropriate formula of medium consist xylane from outer skin of soybean grain and optimum condition of xylanase production. Medium formulate by 36 combinations were made based on variation of ingredients i.e. xylane, polypeptone, and yeast extract. Optimum condition of processing was determined based on combination of shaker speed, temperature, and pH, which produced of highest value of enzyme activity and specific enzyme. Extraction of xylane resulted 32 grams xylane from 1,000 grams outer skin of soybean grain.The best formula of medium for xylanase production using bacteria isolate AIII-5 from outer skin of soybean grain is combination of 0.5% polypeptone, 0.1% of yeast extract, and 1% xylane. The optimum o condition for processing is combination of 120 rpm, 35 C temperature, and pH 9, which resulted the highest value of enzyme activity 366.67 U/ml and enzyme specific activity 612.14 U/mg protein.},
    keywords = {xylanase, outer skin soybean grain},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_388-397_nurrichana_produksi.pdf}
    }
  40. Saraswati, R., D. N. Susilowati, and Elsanti. 2003. Fusi Protoplas Intraspesies antar Bradyrhizobium japonicum. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 398.
    [BibTeX] [Abstract] [PDF: Fusi Protoplas Intraspesies antar Bradyrhizobium japonicum ]
    Genetic improvement of Rhizobia can be carried out by protoplast fusion technique. The aim of this research is to get bacterial protoplast fusion differed from their parental wild types in Nitrogen fixing activity. This intraspecific protoplast fusions was carried out between B. japonicum L17-1kloram and B. japonicum Pd10Abkan. Cells for protoplast preparation were harvested at mid of exponential phase and both isolation and protoplast fusion technique followed Eisa et al method (1995). Lytic of cell wall used lysozyme (a final concentration 5 mg/ml) and fusogenic agent was polyethylene glycol (PEG) 4000 50% (w/v). Protoplast regeneration was done in a non selective medium, but selection of bacterial protoplast fusion was carried out in a selective medium supplemented with kanamycin (200?g/ml) and chloramphenicol (100 ug/ml). Characterization of bacterial protoplast fusion included Gram staining, the growth in a YEMA medium containing bromthymol blue, Congo red absorption, the growth in a YEMA medium containing 2% NaCl, YEMA with pH 4.5 and 9, and Intrinsic Antibiotic Resistance (IAR). Cell for protoplast preparation harvested at 72 hours. The percentage of bacterial protoplast was estimated 72.84% for B. japonicum L17-1kloram and 70.11% for B. japonicum Pd10ABkan. Regeneration frequencies is about 5.35 x 10-3 for B. japonicum L17-1kloram and 7.93 x 10-4 for B. japonicum Pd10ABkan. Moreover the frequency of fusions is about 1.17 x 10-4. Characterization of bacterial protoplast fusion revealed that INTRA-1 into INTRA-30 were rod-shaped Gram negative; did not grow on 2% NaCl; alcaline reacted in a medium containing bromthymol blue and did not absorp Congo red. Bacterial INTRA-1 into INTRA-14 grow in a YEMA medium with pH 4.5 and 9; but INTRA-15; INTRA-16 and INTRA-19 only easily grow in pH 4.5, did not easily grow in pH 9. Moreover bacterial INTRA-17; INTRA-18; INTRA-20 into INTRA-30 grow in pH 4.5, but did not grow in pH 9. Bacterial protoplast fusion can be divided into 6 groupos based on IAR characterization. We got only INTRA-8 gave significant respons to shoot dry weight, root and nodule dry weight and total nitrogen than control. The symbiotic effectivity of INTRA-8 is 70% estimated from control (IoN) and more higher than parental wild types.
    @article{Saraswati03p398,
    title = {{Fusi Protoplas Intraspesies antar Bradyrhizobium japonicum}},
    author = {R. Saraswati and D. N. Susilowati and Elsanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {398},
    year = {2003},
    abstract = { Genetic improvement of Rhizobia can be carried out by protoplast fusion technique. The aim of this research is to get bacterial protoplast fusion differed from their parental wild types in Nitrogen fixing activity. This intraspecific protoplast fusions was carried out between B. japonicum L17-1kloram and B. japonicum Pd10Abkan. Cells for protoplast preparation were harvested at mid of exponential phase and both isolation and protoplast fusion technique followed Eisa et al method (1995). Lytic of cell wall used lysozyme (a final concentration 5 mg/ml) and fusogenic agent was polyethylene glycol (PEG) 4000 50% (w/v). Protoplast regeneration was done in a non selective medium, but selection of bacterial protoplast fusion was carried out in a selective medium supplemented with kanamycin (200?g/ml) and chloramphenicol (100 ug/ml). Characterization of bacterial protoplast fusion included Gram staining, the growth in a YEMA medium containing bromthymol blue, Congo red absorption, the growth in a YEMA medium containing 2% NaCl, YEMA with pH 4.5 and 9, and Intrinsic Antibiotic Resistance (IAR). Cell for protoplast preparation harvested at 72 hours. The percentage of bacterial protoplast was estimated 72.84% for B. japonicum L17-1kloram and 70.11% for B. japonicum Pd10ABkan. Regeneration frequencies is about 5.35 x 10-3 for B. japonicum L17-1kloram and 7.93 x 10-4 for B. japonicum Pd10ABkan. Moreover the frequency of fusions is about 1.17 x 10-4. Characterization of bacterial protoplast fusion revealed that INTRA-1 into INTRA-30 were rod-shaped Gram negative; did not grow on 2% NaCl; alcaline reacted in a medium containing bromthymol blue and did not absorp Congo red. Bacterial INTRA-1 into INTRA-14 grow in a YEMA medium with pH 4.5 and 9; but INTRA-15; INTRA-16 and INTRA-19 only easily grow in pH 4.5, did not easily grow in pH 9. Moreover bacterial INTRA-17; INTRA-18; INTRA-20 into INTRA-30 grow in pH 4.5, but did not grow in pH 9. Bacterial protoplast fusion can be divided into 6 groupos based on IAR characterization. We got only INTRA-8 gave significant respons to shoot dry weight, root and nodule dry weight and total nitrogen than control. The symbiotic effectivity of INTRA-8 is 70% estimated from control (IoN) and more higher than parental wild types.},
    keywords = {Protoplast fusion; intraspecific; B. japonicum; Nitrogen Fixing activityProtoplast fusion; intraspecific; B. japonicum; Nitrogen Fixing activity},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_398-_rasti_fusi.pdf}
    }

2002

  1. Hadiatmi, Tiur S. Silitonga, Sri G. Budiart, and Buang Abdullah. 2002. Eksplorasi Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 1-5.
    [BibTeX] [Abstract] [PDF: Eksplorasi Plasma Nutfah Tanaman Pangan ]
    Agricultural farmland in the Province of Central Java, special region of Yogyakarta, and East Java are almost completed by irrigation. Therefore, almost all the area are grown by high yielding varieties of rice, and as a result local rice are almost extint as well as other cereals and tubers. So, the exploration of food crop germplasm was conducted to collect and conserve the rest of local varieties that left in from several areas in those three provinces. Whithin 14 days exploration in August 2001, from Central and Yogyakarta region have been collected 16 local rice varieties, 73 tubers, 10 local varieties of legumes, and 1 variety of corn, and from East Java collected 18 local rice, 4 tubers, and 7 legumes. All collection are 130 accessions (34 accessions of rice, 1 accession of corn, 17 accessions of legumes, and 78 accessions of tubers).
    @article{Hadiatmi02p1,
    title = {{Eksplorasi Plasma Nutfah Tanaman Pangan}},
    author = {Hadiatmi and Tiur S . Silitonga and Sri G. Budiart and Buang Abdullah},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {1-5},
    year = {2002},
    abstract = {Agricultural farmland in the Province of Central Java, special region of Yogyakarta, and East Java are almost completed by irrigation. Therefore, almost all the area are grown by high yielding varieties of rice, and as a result local rice are almost extint as well as other cereals and tubers. So, the exploration of food crop germplasm was conducted to collect and conserve the rest of local varieties that left in from several areas in those three provinces. Whithin 14 days exploration in August 2001, from Central and Yogyakarta region have been collected 16 local rice varieties, 73 tubers, 10 local varieties of legumes, and 1 variety of corn, and from East Java collected 18 local rice, 4 tubers, and 7 legumes. All collection are 130 accessions (34 accessions of rice, 1 accession of corn, 17 accessions of legumes, and 78 accessions of tubers).},
    keywords = {exploration, food crop, germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_1-5_hadiatmi.pdf}
    }
  2. Somantri, I. H., T. S. Silitonga, N. Zuraida, Minantyorini, S. G. Budiarti, T. Suhartini, S. A. Rais, Hadiatmi, L. Hakim, N. Dewi, and M. Setyowati. 2002. Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 6-43.
    [BibTeX] [Abstract] [PDF: Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan ]
    Conservation done by rejuvenation and seed storage in the right and good way should be done. Nothing do if conservation be done without utilization of its germplasm, so identification of its important traits by characterization on morpho-agronomical characters is the clue. Rejuvenated already done on 750 accessions. of rice; 43 accessions on 14 wild relative of rice; 500 accessions. maize; 600 accessions. soybean; 550 accessions. cassava; 80 accessions. wheat; 209 accessions. sorghum; 600 accessions. ground nut; 300 accessions. mungbean; 100 accessions. minor legume; 912 accessions. sweetpotato as field-conservation and 450 accessions. as pot-conservation; 29 accessions. Canna edulis; 17 accessions. arrow root; 16 accessions. Dioscorea hispida (gadung); 52 accessions. greater yam or Dioscorea alata (ubi kelapa); 13 accessions. Amorphophalus; 30 accessions. lesser yam or Dioscorea esculenta (gembili); and 140 accessions. Taro (Colocasia esculenta) and Tania (Xanthosoma sp.). In vitro conservation were done on some accessions. of cassava, sweetpotato and taro. Morpho- agronomical characterization on all food crops germplasm had high variability on qualitative characters like shape and color, as well as quantitative characters like plant height, tiller number and panicle length. Distinguish charaters were occurs on Rice: Getik Rijal (reg. 5644) had medium maturity (133 days) with 259 seeds/panicle, panicle length 32 cm and plant height 95 cm. Maize: the longest ear (18,2 cm) on Arjuna, the biggest ear diameter on Reg. 2682, the heaviest in 300 seeds (98 g) on Reg. 3686. Five soybean lines had high yield potential and early maturity i.e. B5133 (77 days with 11.9 g/100 seeds), B4220 (77 days with 15.7 g/100 seeds), GM219Si (77 days with 14.3 g/100 seeds), B3076 (77 days with 13.7 g/100 seeds), and Local Ongko-5-1 (74 days with 16.2 g/100 seeds). Some lines with big pod numbers (92 pods/plant) i.e. Reg. 917, Reg. 3702, No. 2810 Si, and B744. The variability were occurs on some leaf characters, stem, and storage root color on cassava germplasm with petiole length variation between 6.0-23.3 cm; leaf lobes width was 1.2-4.8 cm. Plant height were varied from 139-306 cm with variation on the stem diameter 1.3-2.6 cm and the branches height was varied 96-275 cm. Storage root weight was varied from 0.6-3.3 kg/plant with 2-10 storage roots/plant and had harvest index varied from 34-72%. Highrainfall 87, the best in seed weight of wheat (338.4 g) planted in the 3 x 0.5 square meter. Keris, the best performance of sorghum germplasm was very short plant (89 cm) also was short maturity (+82 days) differ from others. Almost 97.3% (576 accessions.) of groundnut had 2 seeds in each pod, only 16 accessions had 3-4 seeds in each pod and 88 accessions had high yield potential (>20 g/plant). Twenty one accessions. of mungbean had good perpormance like short maturity, good plant type, uniform in pod maturing, and seed weight plant 13.4- 18.1g/plant. The highest was on VR 160 (18.1 g/plant). Three accessions. can be harvested on 57 days i.e. 127 VR 127 (Chun Nam-2), VR197 (ML-267), and VR 11. Cowpea germplasm had branch numbers between 3-7 branch/plant maturity 73-88 days, 100 seeds-weight 6-26 g, and pod number 3-18 seeds/pod. Re-characterization on morpho-agronomical characters to validate and to find the duplication on every accession of sweetpotato germplasm was done. From 423 accessions. was harvested on 5.5 m.a.p. and 96 accessions. were no-root storage. Three accessions. of greater yam (Dioscorea alata) or “ubi kelapa” had high yield i.e. No. reg. 36, 601 and 636 with 4.75- 13.0 kg/plant; five accessions of lesser yam (Dioscorea esculenta) or “gembili” which yielded 1.2-2.25 kg/plant i.e. No. reg. 552, 562, 566, 570a and 665, respectively. Three accessions. of arrow root yielded 1.80-1.30 kg/plant i.e. on No. reg. 27, 439, and 504. While, six accessions. of Canna edulis which yielded 2.8-4.47 i.e. No. reg. 57, 87, 135h, 121, 576, and 627. There were some variability on taro germplasm i.e. on leaf edge color, veine pigmentation, sheath color, petiole color, and flesh color. Variation were on leaf width i.e. between 12-44 cm, leaf length: 20-63 cm, total leaf length: 30-117 cm. Plant height were medium (50-100 cm) and height (>100cm). Root weight were range 125-563 g, root length 8.0-16.8 cm, and root diameter 5.7-9.3 cm. Yet, 50 sweetpotato and 10 taro were already in media conservation (MS + manitol 40 g/l) as in vitro conservation.
    @article{Somantri02p6,
    title = {{Rejuvenasi dan Karakterisasi Morfologi Plasma Nutfah Tanaman Pangan}},
    author = {I. H. Somantri and T. S. Silitonga and N. Zuraida and Minantyorini and S. G. Budiarti and T. Suhartini and S. A. Rais and Hadiatmi and L. Hakim and N. Dewi and M. Setyowati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {6-43},
    year = {2002},
    abstract = { Conservation done by rejuvenation and seed storage in the right and good way should be done. Nothing do if conservation be done without utilization of its germplasm, so identification of its important traits by characterization on morpho-agronomical characters is the clue. Rejuvenated already done on 750 accessions. of rice; 43 accessions on 14 wild relative of rice; 500 accessions. maize; 600 accessions. soybean; 550 accessions. cassava; 80 accessions. wheat; 209 accessions. sorghum; 600 accessions. ground nut; 300 accessions. mungbean; 100 accessions. minor legume; 912 accessions. sweetpotato as field-conservation and 450 accessions. as pot-conservation; 29 accessions. Canna edulis; 17 accessions. arrow root; 16 accessions. Dioscorea hispida (gadung); 52 accessions. greater yam or Dioscorea alata (ubi kelapa); 13 accessions. Amorphophalus; 30 accessions. lesser yam or Dioscorea esculenta (gembili); and 140 accessions. Taro (Colocasia esculenta) and Tania (Xanthosoma sp.). In vitro conservation were done on some accessions. of cassava, sweetpotato and taro. Morpho- agronomical characterization on all food crops germplasm had high variability on qualitative characters like shape and color, as well as quantitative characters like plant height, tiller number and panicle length. Distinguish charaters were occurs on Rice: Getik Rijal (reg. 5644) had medium maturity (133 days) with 259 seeds/panicle, panicle length 32 cm and plant height 95 cm. Maize: the longest ear (18,2 cm) on Arjuna, the biggest ear diameter on Reg. 2682, the heaviest in 300 seeds (98 g) on Reg. 3686. Five soybean lines had high yield potential and early maturity i.e. B5133 (77 days with 11.9 g/100 seeds), B4220 (77 days with 15.7 g/100 seeds), GM219Si (77 days with 14.3 g/100 seeds), B3076 (77 days with 13.7 g/100 seeds), and Local Ongko-5-1 (74 days with 16.2 g/100 seeds). Some lines with big pod numbers (92 pods/plant) i.e. Reg. 917, Reg. 3702, No. 2810 Si, and B744. The variability were occurs on some leaf characters, stem, and storage root color on cassava germplasm with petiole length variation between 6.0-23.3 cm; leaf lobes width was 1.2-4.8 cm. Plant height were varied from 139-306 cm with variation on the stem diameter 1.3-2.6 cm and the branches height was varied 96-275 cm. Storage root weight was varied from 0.6-3.3 kg/plant with 2-10 storage roots/plant and had harvest index varied from 34-72%. Highrainfall 87, the best in seed weight of wheat (338.4 g) planted in the 3 x 0.5 square meter. Keris, the best performance of sorghum germplasm was very short plant (89 cm) also was short maturity (+82 days) differ from others. Almost 97.3% (576 accessions.) of groundnut had 2 seeds in each pod, only 16 accessions had 3-4 seeds in each pod and 88 accessions had high yield potential (>20 g/plant). Twenty one accessions. of mungbean had good perpormance like short maturity, good plant type, uniform in pod maturing, and seed weight plant 13.4- 18.1g/plant. The highest was on VR 160 (18.1 g/plant). Three accessions. can be harvested on 57 days i.e. 127 VR 127 (Chun Nam-2), VR197 (ML-267), and VR 11. Cowpea germplasm had branch numbers between 3-7 branch/plant maturity 73-88 days, 100 seeds-weight 6-26 g, and pod number 3-18 seeds/pod. Re-characterization on morpho-agronomical characters to validate and to find the duplication on every accession of sweetpotato germplasm was done. From 423 accessions. was harvested on 5.5 m.a.p. and 96 accessions. were no-root storage. Three accessions. of greater yam (Dioscorea alata) or “ubi kelapa” had high yield i.e. No. reg. 36, 601 and 636 with 4.75- 13.0 kg/plant; five accessions of lesser yam (Dioscorea esculenta) or “gembili” which yielded 1.2-2.25 kg/plant i.e. No. reg. 552, 562, 566, 570a and 665, respectively. Three accessions. of arrow root yielded 1.80-1.30 kg/plant i.e. on No. reg. 27, 439, and 504. While, six accessions. of Canna edulis which yielded 2.8-4.47 i.e. No. reg. 57, 87, 135h, 121, 576, and 627. There were some variability on taro germplasm i.e. on leaf edge color, veine pigmentation, sheath color, petiole color, and flesh color. Variation were on leaf width i.e. between 12-44 cm, leaf length: 20-63 cm, total leaf length: 30-117 cm. Plant height were medium (50-100 cm) and height (>100cm). Root weight were range 125-563 g, root length 8.0-16.8 cm, and root diameter 5.7-9.3 cm. Yet, 50 sweetpotato and 10 taro were already in media conservation (MS + manitol 40 g/l) as in vitro conservation.},
    keywords = {Food crops, germplasm, rejuvenation, characterization},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_6-43_idahanarida.pdf}
    }
  3. Budiarti, S. G., Suyono, D. Koswanudin, I. H. Somantri, and T. S. Silitonga. 2002. Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Hama. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 44-51.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Hama ]
    Brownplanthopper, Nilaparvata lugens St{å{}}l that attack on rice, and seedling fly Atherigona exigua on maize, both are important pests that cause yield lost. The easy method, cheap and save to take care the problem use of resistant varieties. The objective of the experiment were to know the resistance degree from rice and maize germplasm in RIFCB Gen Bank. Evaluation on rice germplasm to brown planthopper conducted at green house, RIFCB, in June 2001-February 2002. Five hundred accessions of rice germplasm were tested to BPH IR42 and IR64 population. Each accession were planted irone row which consisted of 15-20 seed in the wood seedling nursery, with spacing +2.5 cm. Infestation with nimph BPH instar conducted at 7 day old seedling, and then put in a cage from gauze plastic. Resistance evaluation was done after IR42 or IR64 (susceptible varieties) died (90%) with score 7-9. Evaluation based on IRRI standard evaluation system. Evaluation on maize germplasm conducted at Cikeumeuh Research Instalation, Bogor from November 2001-January 2002. Seventy five accessions of maize germplasm were tested using RCBD design with 2 replications. As check varieties were used Arjuna as a susceptible variety and Sadewa as a resistant variety. Plot size 1 m x 5 m (spacing 25 cm x 20 cm, 4 rows) per accessions. The result showed that there were 54 resistant accessions (score 2-3) of rice germplasm were tested to WBC IR42 population, and six aces resistant to WBC IR64 population (score 3-0). Evaluation on maize to seedling fly found 12 resistant acc some of them that hove the lowest attacked intensity was Pena Mais (Reg. 1621), the highest attacked intensity were Kapas (Reg. 2000), and Doke (Reg. 2001).
    @article{Budiarti02p44,
    title = {{Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Hama}},
    author = {S. G. Budiarti and Suyono and D. Koswanudin and I. H. Somantri and T. S. Silitonga},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {44-51},
    year = {2002},
    abstract = { Brownplanthopper, Nilaparvata lugens St{\aa{}}l that attack on rice, and seedling fly Atherigona exigua on maize, both are important pests that cause yield lost. The easy method, cheap and save to take care the problem use of resistant varieties. The objective of the experiment were to know the resistance degree from rice and maize germplasm in RIFCB Gen Bank. Evaluation on rice germplasm to brown planthopper conducted at green house, RIFCB, in June 2001-February 2002. Five hundred accessions of rice germplasm were tested to BPH IR42 and IR64 population. Each accession were planted irone row which consisted of 15-20 seed in the wood seedling nursery, with spacing +2.5 cm. Infestation with nimph BPH instar conducted at 7 day old seedling, and then put in a cage from gauze plastic. Resistance evaluation was done after IR42 or IR64 (susceptible varieties) died (90%) with score 7-9. Evaluation based on IRRI standard evaluation system. Evaluation on maize germplasm conducted at Cikeumeuh Research Instalation, Bogor from November 2001-January 2002. Seventy five accessions of maize germplasm were tested using RCBD design with 2 replications. As check varieties were used Arjuna as a susceptible variety and Sadewa as a resistant variety. Plot size 1 m x 5 m (spacing 25 cm x 20 cm, 4 rows) per accessions. The result showed that there were 54 resistant accessions (score 2-3) of rice germplasm were tested to WBC IR42 population, and six aces resistant to WBC IR64 population (score 3-0). Evaluation on maize to seedling fly found 12 resistant acc some of them that hove the lowest attacked intensity was Pena Mais (Reg. 1621), the highest attacked intensity were Kapas (Reg. 2000), and Doke (Reg. 2001).},
    keywords = {Evaluation, germplasm, insect pest},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_44-51_srigajatri.pdf}
    }
  4. Rais, S. A., T. S. Silitonga, S. G. Budiarti, and A. Nasution. 2002. Evaluasi Ketahanan Plasma Nutfah Padi dan Jagung terhadap Penyakit. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 52-62.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Padi dan Jagung terhadap Penyakit ]
    The experiments were aimed to find out some germplasm of rice resistant/ tolerant to blast and bacterial leaf blight, and some germplasm of corn resistant to downy mildew disease. A number of 250 accession of rice and 200 accessions of corn were evaluated in a green house and research instalation in Cikeumeuh and Sukabumi during rainy season on 2001. The experiment was arranged in randomized block design with 2-3 replications. The accessions were grown at plant spacing of 25 cm x 25 cm for rice and 50 cm x 20 cm for corn. Fertilization for rice in the field 200 kg urea + 100 kg TSP + 100 kg KCl, 1/3 dosie of Urea, TSP, and KCl were applied at planting time; 2/3 dosis of urea were applied at 4 and 7 weeks after planting. Fertilizier in green house at the rate 2 g urea, 0.6 g TSP, and 0.6 g KCl per pot, inoculation of diseases at 60 days after planting, by cut 5 cm from the top of rice leaf. Skoring 1-9 for BLB observe in 21 days after inoculation. Skoring 1-9 for blast observed in 40 and 60 days, and neck blast in. Inoculation of spora downy mildew suspention 4 days after planting of corn and skoring 1-5 while observed at 21, 28, and 35 days after planting. The results showeding 14 accessions of rice germplasm were resistant to BLB group IV and VIII, while 46 accessions were resistant to leaf blast and 4 accessions of corn germplasm were resistant to downy mildew.
    @article{Rais02p52,
    title = {{Evaluasi Ketahanan Plasma Nutfah Padi dan Jagung terhadap Penyakit}},
    author = {S. A. Rais and T. S. Silitonga and S. G. Budiarti and A. Nasution},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {52-62},
    year = {2002},
    abstract = {The experiments were aimed to find out some germplasm of rice resistant/ tolerant to blast and bacterial leaf blight, and some germplasm of corn resistant to downy mildew disease. A number of 250 accession of rice and 200 accessions of corn were evaluated in a green house and research instalation in Cikeumeuh and Sukabumi during rainy season on 2001. The experiment was arranged in randomized block design with 2-3 replications. The accessions were grown at plant spacing of 25 cm x 25 cm for rice and 50 cm x 20 cm for corn. Fertilization for rice in the field 200 kg urea + 100 kg TSP + 100 kg KCl, 1/3 dosie of Urea, TSP, and KCl were applied at planting time; 2/3 dosis of urea were applied at 4 and 7 weeks after planting. Fertilizier in green house at the rate 2 g urea, 0.6 g TSP, and 0.6 g KCl per pot, inoculation of diseases at 60 days after planting, by cut 5 cm from the top of rice leaf. Skoring 1-9 for BLB observe in 21 days after inoculation. Skoring 1-9 for blast observed in 40 and 60 days, and neck blast in. Inoculation of spora downy mildew suspention 4 days after planting of corn and skoring 1-5 while observed at 21, 28, and 35 days after planting. The results showeding 14 accessions of rice germplasm were resistant to BLB group IV and VIII, while 46 accessions were resistant to leaf blast and 4 accessions of corn germplasm were resistant to downy mildew.},
    keywords = {Germplasm, blast, bacterial leaf blight},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_52-62_sriastutirais.pdf}
    }
  5. Suhartini, T., S. G. B. udiarti, T. S. Silitonga, N. Dewi, Hadiatmi, S. A. Rais, and I. H. Somantri. 2002. Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Kekeringan, Keracunan Al dan Fe). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 63-76.
    [BibTeX] [Abstract] [PDF: Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Kekeringan, Keracunan Al dan Fe) ]
    In 2001 the activities have been evaluated 3 crops germplasm, there were rice, corn and soybean to drought and Al toxicity tested and to Fe toxicity for rice. The location for testing drought tolerant was in Imogiri, Central Java in DS 2001 and Tamanbogo Lampung for testing Al dan Fe toxicity in WS 2001. The total accessions number for rice drought test were 250 accessions, 250 accessions for testing to Al toxicity and 200 accessions for testing to iron toxicity. The total accessions for corn and soybean are 100 respectively for testing to drought and Al toxicity. The aim of these activities were to get varieties that tolerant to those problems. The result showed that 9 accessions of rice germplasm tolerant to drought with score 1 and good vigor, 140 accessions are moderat tolerant with score 3 -5. Some of rice germplasm tolerant to drought are Ramos, Sipulau, Sidapat B, Cimanuk, and Ketan Berantai. For soybean germplasm were selected 10 accessions tolerant to drought with good performance and the yield of seed was the same with No. 29 as the tolerant check. The selected number were B3737, B3507, and B 3556 with yielded higher than No. 29. The selected corn germplasm to drought were 8 accessions, there were Pool5 G18(S)C3SK-9-1-1, Pool4G19(S)C2SK-31-1-1, Petak (3151), Lokal Lempuyang Luhur, Lokal Semaya (3198), Beak 3064, Nakula, and Lokal Gerung (3119). The rice germplasm selected in Al toxicity (Al saturated were 48,32-56,95%) were 4 accessions with score 1 (very tolerant) they were Rumbai (Reg. 20968), Seni Kuku Belalang (Reg. 21102), Seni Pesak (Reg. 21105), and Pulut Unggul (Reg. 21110), and 34 accessions were tolerant (score 3). The selected rice germ-plasm to iron toxicity were 45 accecions that tolerant with score 1-3, and 61 accessions were score 5 (moderate). Some of selected rice germplasm to iron toxicity were Bawang, Aceh, Dayang, Ketan Buluh, Cempo Manggar, Kakaran, Blumbungan, Pare Ketek, Limboto, Gadis Putih, and Pulut Ngketi. Some of selected corn germplasm to Al toxicity were 7 accessions, there were Lokal (3313), Improved Tiniquib, Jagung Ketan (3515), Bulareqet (Jagung Pulut) Birolle Goasa, Birolle Kamo, and P5G8 (10F) E. The soybean germplasm tolerant to Al toxicity were selected 10 accessions with score 1-3, some of them are B3470, B3097, B4369, B3593, B3610, B3659, and GM 119Si.
    @article{Suhartini02p63,
    title = {{Evaluasi Toleransi Plasma Nutfah Padi, Jagung, dan Kedelai terhadap Lahan Bermasalah (Kekeringan, Keracunan Al dan Fe)}},
    author = {T. Suhartini and S. G. B. udiarti and T. S. Silitonga and N. Dewi and Hadiatmi and S. A. Rais and I. H. Somantri},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {63-76},
    year = {2002},
    abstract = { In 2001 the activities have been evaluated 3 crops germplasm, there were rice, corn and soybean to drought and Al toxicity tested and to Fe toxicity for rice. The location for testing drought tolerant was in Imogiri, Central Java in DS 2001 and Tamanbogo Lampung for testing Al dan Fe toxicity in WS 2001. The total accessions number for rice drought test were 250 accessions, 250 accessions for testing to Al toxicity and 200 accessions for testing to iron toxicity. The total accessions for corn and soybean are 100 respectively for testing to drought and Al toxicity. The aim of these activities were to get varieties that tolerant to those problems. The result showed that 9 accessions of rice germplasm tolerant to drought with score 1 and good vigor, 140 accessions are moderat tolerant with score 3 -5. Some of rice germplasm tolerant to drought are Ramos, Sipulau, Sidapat B, Cimanuk, and Ketan Berantai. For soybean germplasm were selected 10 accessions tolerant to drought with good performance and the yield of seed was the same with No. 29 as the tolerant check. The selected number were B3737, B3507, and B 3556 with yielded higher than No. 29. The selected corn germplasm to drought were 8 accessions, there were Pool5 G18(S)C3SK-9-1-1, Pool4G19(S)C2SK-31-1-1, Petak (3151), Lokal Lempuyang Luhur, Lokal Semaya (3198), Beak 3064, Nakula, and Lokal Gerung (3119). The rice germplasm selected in Al toxicity (Al saturated were 48,32-56,95%) were 4 accessions with score 1 (very tolerant) they were Rumbai (Reg. 20968), Seni Kuku Belalang (Reg. 21102), Seni Pesak (Reg. 21105), and Pulut Unggul (Reg. 21110), and 34 accessions were tolerant (score 3). The selected rice germ-plasm to iron toxicity were 45 accecions that tolerant with score 1-3, and 61 accessions were score 5 (moderate). Some of selected rice germplasm to iron toxicity were Bawang, Aceh, Dayang, Ketan Buluh, Cempo Manggar, Kakaran, Blumbungan, Pare Ketek, Limboto, Gadis Putih, and Pulut Ngketi. Some of selected corn germplasm to Al toxicity were 7 accessions, there were Lokal (3313), Improved Tiniquib, Jagung Ketan (3515), Bulareqet (Jagung Pulut) Birolle Goasa, Birolle Kamo, and P5G8 (10F) E. The soybean germplasm tolerant to Al toxicity were selected 10 accessions with score 1-3, some of them are B3470, B3097, B4369, B3593, B3610, B3659, and GM 119Si.},
    keywords = { Rice, corn, soybean, germplasm, drought, Al and Fe toxicity},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_63-76_tintinsuhartini.pdf}
    }
  6. Zuraida, N., I. H. Somantri, T. S. Silitonga, S. G. Budiarti, Hadiatmi, Minantyorini, S. Widowati, and A. Hidayat. 2002. Evaluasi Sifat Fisiko Kimia dan Fungsional Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 77-84.
    [BibTeX] [Abstract] [PDF: Evaluasi Sifat Fisiko Kimia dan Fungsional Plasma Nutfah Tanaman Pangan ]
    Genetic diversity of food crops germplasm had a great role in plant improvement, as diversity of nutrion content is very important to plant improvement for high nutrition content. Analysis of nutrition qualities had done in Biokimia dan Enzimatik Laboratory, RIFCB, 2001 to amylose content in rice and maize, starch content in sweetpotato, cassava, edible canna, and Dioscorea sp., tanin content in sorghum and HCN content in cassava. Nutrition analysis for soybean were protein and fat content, water content, ash content, fiber content, stearic acid, palmitic acid, oleic acid, linoleic acid, and linolenic acid. The results showed that variation of amylose content is 16.4-29.7% in rice and 10.2-30.8% in maize. Sweetpotato had starch content between 28.0-51.7%, cassava (28.0-51.7%), edible canna (31.3-38.9%), and Dioscorea sp. (14.0-62.3%), sorghum had tanin content between 0.12-0.85%. HCN content in cassava had variation between 8.3-150.5 ppm for cassava root and 59.4532.6 ppm for cassava leaf. Soybean had fat content between 18.92-29.62% and 35.91-40.10% for protein content, 3.04-4.32% for ash content, 2.88-3.15% for fiber content, 8.9-11.2% for water content, 3.26-4.01% for stearic acid, 7.86-13.43% for palmitic acid, 20.68-33.52% for oleic acid, 38.83-46.58% for linoleic acid, and 4.528.94% for linolenic acid.
    @article{Zuraida02p77,
    title = {{Evaluasi Sifat Fisiko Kimia dan Fungsional Plasma Nutfah Tanaman Pangan}},
    author = {N. Zuraida and I. H. Somantri and T. S. Silitonga and S. G. Budiarti and Hadiatmi and Minantyorini and S. Widowati and A. Hidayat},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {77-84},
    year = {2002},
    abstract = {Genetic diversity of food crops germplasm had a great role in plant improvement, as diversity of nutrion content is very important to plant improvement for high nutrition content. Analysis of nutrition qualities had done in Biokimia dan Enzimatik Laboratory, RIFCB, 2001 to amylose content in rice and maize, starch content in sweetpotato, cassava, edible canna, and Dioscorea sp., tanin content in sorghum and HCN content in cassava. Nutrition analysis for soybean were protein and fat content, water content, ash content, fiber content, stearic acid, palmitic acid, oleic acid, linoleic acid, and linolenic acid. The results showed that variation of amylose content is 16.4-29.7% in rice and 10.2-30.8% in maize. Sweetpotato had starch content between 28.0-51.7%, cassava (28.0-51.7%), edible canna (31.3-38.9%), and Dioscorea sp. (14.0-62.3%), sorghum had tanin content between 0.12-0.85%. HCN content in cassava had variation between 8.3-150.5 ppm for cassava root and 59.4532.6 ppm for cassava leaf. Soybean had fat content between 18.92-29.62% and 35.91-40.10% for protein content, 3.04-4.32% for ash content, 2.88-3.15% for fiber content, 8.9-11.2% for water content, 3.26-4.01% for stearic acid, 7.86-13.43% for palmitic acid, 20.68-33.52% for oleic acid, 38.83-46.58% for linoleic acid, and 4.528.94% for linolenic acid.},
    keywords = {nutrition, diversity, food crops germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_77-84_nanizuraida.pdf}
    }
  7. Silitonga, T. S., Asadi, and H. Siregar. 2002. Studi Genetik Ukuran Biji Padi dan Ketahanan Kedelai terhadap Virus Kerdil. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 85-95.
    [BibTeX] [Abstract] [PDF: Studi Genetik Ukuran Biji Padi dan Ketahanan Kedelai terhadap Virus Kerdil ]
    This study was conducted from April to December 2001 at green house, RIFCB Bogor and Sukamandi Research Station. The crossing between big grain with medium and small size were conducted to study the mode of inheritance in grain size. Ten cross combinations along with their reciprocals were also made in soybean which resistant, intermediate and susceptible to SSV. All the seeds which consist of parents, F1 and F2 seeds were already grown to study the inheritance of the grain size in rice and resistance to SSV in soybean. There was no maternal effect found in the soybean crossing between resistant and susceptible. Resistance to SSV in Mlg 2521 was caused by a dominant gene, while in B3570 and Taichung were caused by 2 major genes with interaction of duplicate recessive efistatic.
    @article{Silitonga02p85,
    title = {{Studi Genetik Ukuran Biji Padi dan Ketahanan Kedelai terhadap Virus Kerdil}},
    author = {T. S. Silitonga and Asadi and H. Siregar},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {85-95},
    year = {2002},
    abstract = {This study was conducted from April to December 2001 at green house, RIFCB Bogor and Sukamandi Research Station. The crossing between big grain with medium and small size were conducted to study the mode of inheritance in grain size. Ten cross combinations along with their reciprocals were also made in soybean which resistant, intermediate and susceptible to SSV. All the seeds which consist of parents, F1 and F2 seeds were already grown to study the inheritance of the grain size in rice and resistance to SSV in soybean. There was no maternal effect found in the soybean crossing between resistant and susceptible. Resistance to SSV in Mlg 2521 was caused by a dominant gene, while in B3570 and Taichung were caused by 2 major genes with interaction of duplicate recessive efistatic.},
    keywords = {inheritance, ssv, rice, soybean},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_85-95_sudiaty.pdf}
    }
  8. Minantyorini, Hakim Kurniawan, Mamik Setyowati, Tiur S. Silitonga, Hadiatmi, Sri G. Budiarti, Sri A. Rais, Nani Zuraida, Lukman Hakim, Sutoro, Asadi, and Tintin Suhartini. 2002. Pengembangan Database Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 96-101.
    [BibTeX] [Abstract] [PDF: Pengembangan Database Plasma Nutfah Tanaman Pangan ]
    The documentation of morphoagronomical data of food crops germplasm was started several years ago and step by step go to the ideal database by new entry of new characters or new accession recorded on each commodity. This is done by using Microsoft Access, Microsoft Excell, and dBase. On 2001 activity, 3 258 accessions of rice, 705 accessions of maize, 771 accessions of soybean, 110 of cassava, 912 sweetpotato, 1024 accessions of groundnut, 1029 accessions of mungbean, and 174 accessions of sorghum. Data entry on rice and sweetpotato germplasm was increase due to some new collection. Data validation and entry always done to reach the ideal database on food crops germplasm.
    @article{Minantyorini02p96,
    title = {{Pengembangan Database Plasma Nutfah Tanaman Pangan}},
    author = {Minantyorini and Hakim Kurniawan and Mamik Setyowati and Tiur S. Silitonga and Hadiatmi and Sri G. Budiarti and Sri A. Rais and Nani Zuraida and Lukman Hakim and Sutoro and Asadi and Tintin Suhartini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {96-101},
    year = {2002},
    abstract = {The documentation of morphoagronomical data of food crops germplasm was started several years ago and step by step go to the ideal database by new entry of new characters or new accession recorded on each commodity. This is done by using Microsoft Access, Microsoft Excell, and dBase. On 2001 activity, 3 258 accessions of rice, 705 accessions of maize, 771 accessions of soybean, 110 of cassava, 912 sweetpotato, 1024 accessions of groundnut, 1029 accessions of mungbean, and 174 accessions of sorghum. Data entry on rice and sweetpotato germplasm was increase due to some new collection. Data validation and entry always done to reach the ideal database on food crops germplasm.},
    keywords = {database, germplasm, food crop, development},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_96-101_minantyorini.pdf}
    }
  9. Somantri, I. H., Dinar A. Ambarwati, I. S. Dewi, A. Apriana, and T. J. Santoso. 2002. Transformasi Padi dengan Bombardmen Mikroprojektil. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 102-110.
    [BibTeX] [Abstract] [PDF: Transformasi Padi dengan Bombardmen Mikroprojektil ]
    The research was carried out in the Molecular Biology Laboratory, Molecular Biology Division, RIFCB, Bogor, in year 2001. The aim of the research is to produce transgenic rice resistant to stem borer. The transformation technique was carried out by means of particle bombardment using plasmid pUBB and pUBC that had been co-transformed with plasmid pRQ6. Plasmid pUBB and pUBC contain the cryIAb and cryIAc, respectively. These two genes are the resistant gene against to stem borer. Plasmid pRQ6 contains the genes responsible as a reporter gene (gus gene) and selectable marker (hph gene). The procedure of bombardment is carried by two ways, i.e. by Fauquet et al. (1996) and Sawant et al. (2000). The results showed (1) 30 calli Taipei-309 containing cryIAb gene and 1 callus Taipei-309 containing cryIAc regenerated 283 putative transgenic plants, (2) Regeneration media RNB (NB + BAP 4 mg/l + NAA 0.8 mg/l) and NK1N0.5 (NB + kinetin 1 mg/l + NAA 0.5 mg/l) could produce planlets from the transformed calli, (3) The procedure of bombardment by Sawant et al. (2000) using gold particles incubated in 180oC overnight and isopropanol-treated plasmid DNA is more effective than the one by Fauquet et al. (1996), (4) The gus assay result show 7 (9.46%) out of 74 assayed plant T0 are positive.
    @article{Somantri02p102,
    title = {{Transformasi Padi dengan Bombardmen Mikroprojektil}},
    author = {I. H. Somantri and A. Dinar Ambarwati and I. S. Dewi and A. Apriana and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {102-110},
    year = {2002},
    abstract = {The research was carried out in the Molecular Biology Laboratory, Molecular Biology Division, RIFCB, Bogor, in year 2001. The aim of the research is to produce transgenic rice resistant to stem borer. The transformation technique was carried out by means of particle bombardment using plasmid pUBB and pUBC that had been co-transformed with plasmid pRQ6. Plasmid pUBB and pUBC contain the cryIAb and cryIAc, respectively. These two genes are the resistant gene against to stem borer. Plasmid pRQ6 contains the genes responsible as a reporter gene (gus gene) and selectable marker (hph gene). The procedure of bombardment is carried by two ways, i.e. by Fauquet et al. (1996) and Sawant et al. (2000). The results showed (1) 30 calli Taipei-309 containing cryIAb gene and 1 callus Taipei-309 containing cryIAc regenerated 283 putative transgenic plants, (2) Regeneration media RNB (NB + BAP 4 mg/l + NAA 0.8 mg/l) and NK1N0.5 (NB + kinetin 1 mg/l + NAA 0.5 mg/l) could produce planlets from the transformed calli, (3) The procedure of bombardment by Sawant et al. (2000) using gold particles incubated in 180oC overnight and isopropanol-treated plasmid DNA is more effective than the one by Fauquet et al. (1996), (4) The gus assay result show 7 (9.46%) out of 74 assayed plant T0 are positive.},
    keywords = {rice transformation, pubb, pubc, particle bombardment},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_102-110_idahanarida.pdf}
    }
  10. Ambarwati, A. D., A. Sisharmini, T. J. Santoso, M. Herman, and Minantyorini. 2002. Transformasi Ubi Jalar dengan Gen pinII dan Gen CP-SPFMV. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 111-117.
    [BibTeX] [Abstract] [PDF: Transformasi Ubi Jalar dengan Gen pinII dan Gen CP-SPFMV ]
    The production of transgenic sweet potato resistant to pest and virus diseases can be done by genetic enginering through transformation techniques. In the year 2001 the experiment on transformation of sweet potato resistant to weevil or virus disease was conducted. Transformation was performed by introducting proteinase inhibitor (pinII) gene or coat protein-SPFMV gene with transformation techniques of particle bombardment and Agrobacterium tumefaciens. Transfor-mation through particle bombarment used plasmid pTWa (pinII, bar), whereas through A. tumefaciens used strain LBA4404 containing pGA643 pin ( pinII, nptII) or binary plasmid pMON10574-1 and pMON10575-1 containing CP-SPFMV, gus, and nptII gene. Explants from petiole and leaf pieces of Sweet potato cv. Jewel were used as target tissue. Pre-treatment of explant before transformation by soaking in A3 liquid media (1/10 MS basal salt, 212.2 mg/l galacturonic acid, 3.9 g/l MES, 30 g/l sucrose) can increase transformation efficiency as much as 20-56%. So far, transformation through particle bombardment produced 1 shoot transformant that is suspected to contain pinII gene, whereas through A. tumefaciens produced 13 putative transgenic plants transformed with pinII gene and 6 putative transgenic plants with CP-SPFMV gene.
    @article{Ambarwati02p111,
    title = {{Transformasi Ubi Jalar dengan Gen pinII dan Gen CP-SPFMV}},
    author = {A. D. Ambarwati and A. Sisharmini and T. J. Santoso and M. Herman and Minantyorini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {111-117},
    year = {2002},
    abstract = {The production of transgenic sweet potato resistant to pest and virus diseases can be done by genetic enginering through transformation techniques. In the year 2001 the experiment on transformation of sweet potato resistant to weevil or virus disease was conducted. Transformation was performed by introducting proteinase inhibitor (pinII) gene or coat protein-SPFMV gene with transformation techniques of particle bombardment and Agrobacterium tumefaciens. Transfor-mation through particle bombarment used plasmid pTWa (pinII, bar), whereas through A. tumefaciens used strain LBA4404 containing pGA643 pin ( pinII, nptII) or binary plasmid pMON10574-1 and pMON10575-1 containing CP-SPFMV, gus, and nptII gene. Explants from petiole and leaf pieces of Sweet potato cv. Jewel were used as target tissue. Pre-treatment of explant before transformation by soaking in A3 liquid media (1/10 MS basal salt, 212.2 mg/l galacturonic acid, 3.9 g/l MES, 30 g/l sucrose) can increase transformation efficiency as much as 20-56%. So far, transformation through particle bombardment produced 1 shoot transformant that is suspected to contain pinII gene, whereas through A. tumefaciens produced 13 putative transgenic plants transformed with pinII gene and 6 putative transgenic plants with CP-SPFMV gene.},
    keywords = {transformation, sweet potato, pinii gene, cp-spfmv gene},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_111-117_dinar.pdf}
    }
  11. Damayanti, D., Sutrisno, S. J. Pardal, M. Herman, Ekramli, R. Sundasari, and E. Ibrahim. 2002. Bioasai Tanaman Kedelai Transgenik R2 terhadap Etiella zinckenella Tr.. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 118-124.
    [BibTeX] [Abstract] [PDF: Bioasai Tanaman Kedelai Transgenik R2 terhadap Etiella zinckenella Tr. ]
    Bioassay on R2 generation of transgenic soybean plants to pod borer (Etiella zinckenella, Tr.) has been conducted at Biosafety Containment of RIFCB in year 2001. The objective of this assay was to obtain transgenic soybean plants resistant to pod borer. Transgenic soybean seeds of TP1 and TP2 events were planted in greenhouse of Biosafety Containment. Fifty days after plantation, each plant was sampled ten pods for bioassay. Each pod was infested with 1-3 neonate larvae of pod borer and then covered with small pores plastic bag (to avoid escaped larvae). Observation was carried out on pods before harvesting. The observation was focus on the percentage of larva mortality, pod, and seed damages, number of undamaged seeds, larvae stages or development (number of imago). Result indicated that all 21 transgenic soybean plants were more resistant to pod borer larvae compared to control plants. The range of larvae mortality were 50-90% with average 76.9%, the length of larvae were 3.6-7 mm (average = 5.1 mm). The percentage of undamaged seeds were 35-70% (average = 57.7%) and the damaged seeds were 30 -65% (average = 42.3%) while the control plants has 27.5% larvae mortality, the length of larvae 9.45 mm, undamaged seeds only 7.5% and damaged seeds 92.5%. This result should be confirm with molecular analysis to ensure the transgenic plants.
    @article{Damayanti02p118,
    title = {{Bioasai Tanaman Kedelai Transgenik R2 terhadap Etiella zinckenella Tr.}},
    author = {D. Damayanti and Sutrisno and S. J. Pardal and M. Herman and Ekramli and R. Sundasari and E. Ibrahim},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {118-124},
    year = {2002},
    abstract = { Bioassay on R2 generation of transgenic soybean plants to pod borer (Etiella zinckenella, Tr.) has been conducted at Biosafety Containment of RIFCB in year 2001. The objective of this assay was to obtain transgenic soybean plants resistant to pod borer. Transgenic soybean seeds of TP1 and TP2 events were planted in greenhouse of Biosafety Containment. Fifty days after plantation, each plant was sampled ten pods for bioassay. Each pod was infested with 1-3 neonate larvae of pod borer and then covered with small pores plastic bag (to avoid escaped larvae). Observation was carried out on pods before harvesting. The observation was focus on the percentage of larva mortality, pod, and seed damages, number of undamaged seeds, larvae stages or development (number of imago). Result indicated that all 21 transgenic soybean plants were more resistant to pod borer larvae compared to control plants. The range of larvae mortality were 50-90% with average 76.9%, the length of larvae were 3.6-7 mm (average = 5.1 mm). The percentage of undamaged seeds were 35-70% (average = 57.7%) and the damaged seeds were 30 -65% (average = 42.3%) while the control plants has 27.5% larvae mortality, the length of larvae 9.45 mm, undamaged seeds only 7.5% and damaged seeds 92.5%. This result should be confirm with molecular analysis to ensure the transgenic plants. },
    keywords = {Bioassay, transgenic soybean plants, resistance, pod borer (Etiella zinckenella Tr.)},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_118-124_diani.pdf}
    }
  12. Hadiarto, T., T. I. R. Utami, S. J. Pardal, and M. Herman. 2002. Analisis Molekuler Gen pinII pada Tanaman Kedelai Transgenik R2. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 133-140.
    [BibTeX] [Abstract] [PDF: Analisis Molekuler Gen pinII pada Tanaman Kedelai Transgenik R2 ]
    Objectives of this research is molecular analysis of pinII gene in transgenic soybean R1 and R2. 127 plant samples of WP2 (Wilis variety with gene gun treatment, R1), 18 plant samples of WP1 (Wilis variety with gene gun treatment, R2), 9 plant samples of TP1 and 16 samples of TP2 (Tidar variety with gene gun treatment, R2) and 27 plant samples of AT1 (Tidar variety with Agrobacterium treatment, R2) and 2 non transgenic plants were isolated DNA. The presence of pinII gene in the plants is examined by means of PCR and gel electrophoresis. A pair of specific primers that can detect the pinII gene is used in the PCR. The controls for the PCR applies DNA of non-transformed soybean (as negative control), ddH2O (as negative control) and plasmid pTWa that contains pinII gene (as positive control). The analysis results show that there are 11 plant DNA samples that produce DNA fragment of 600 bp. These samples are from WP2 and AT1.
    @article{Hadiarto02p133,
    title = {{Analisis Molekuler Gen pinII pada Tanaman Kedelai Transgenik R2}},
    author = {T. Hadiarto and T. I. R. Utami and S. J. Pardal and M. Herman},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {133-140},
    year = {2002},
    abstract = {Objectives of this research is molecular analysis of pinII gene in transgenic soybean R1 and R2. 127 plant samples of WP2 (Wilis variety with gene gun treatment, R1), 18 plant samples of WP1 (Wilis variety with gene gun treatment, R2), 9 plant samples of TP1 and 16 samples of TP2 (Tidar variety with gene gun treatment, R2) and 27 plant samples of AT1 (Tidar variety with Agrobacterium treatment, R2) and 2 non transgenic plants were isolated DNA. The presence of pinII gene in the plants is examined by means of PCR and gel electrophoresis. A pair of specific primers that can detect the pinII gene is used in the PCR. The controls for the PCR applies DNA of non-transformed soybean (as negative control), ddH2O (as negative control) and plasmid pTWa that contains pinII gene (as positive control). The analysis results show that there are 11 plant DNA samples that produce DNA fragment of 600 bp. These samples are from WP2 and AT1.},
    keywords = {molecular analysis, pinii gene, transgenic soybean r2},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_133-140_totohadiarto.pdf}
    }
  13. Dewi, I. S., I. H. Somantri, D. Damayanti, A. Apriana, and T. J. Santoso. 2002. Evaluasi Tanaman Padi Transgenik Balitbio terhadap Hama Penggerek Batang. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 141-149.
    [BibTeX] [Abstract] [PDF: Evaluasi Tanaman Padi Transgenik Balitbio terhadap Hama Penggerek Batang ]
    The success in transgenic plants production occurred when plants express the inserted gene and interest new phenotype appears. Research team in RIFCB had succeeded in making putative rice transgenic plants containing cryIA(b) gene. The long-term objective of the research is to obtain transgenic rice resistance to stem borer. The observation in putative transgenic T1 Taipei-309 on sundep (dead-hearts) indicated that several plants were highly resistance (10 plants of T-1C and 1 plant of T-2H), resistance (3 plants of T-1C), and moderately resistance (2 plants of T-1C, 2 plants of T -1A, and 1 plant of T -2H). Others showed moderately susceptible (8 plants) and susceptible (5 plants), while 16 plants were highly susceptible to stem borer attack at vegetative phase. The observation on beluk (white-heads) indicated that several plants were highly resistance (15 plants of T-1C and 1 plant of T-2H). Only 1 plant of T-2H was moderately resistance. No plants can be identified as resistance. Several other plants were moderately susceptible (6 plants), susceptible (7 plants), and highly susceptible (26 plants). The research in bioassay of other putative transgenic rice was still on going.
    @article{Dewi02p141,
    title = {{Evaluasi Tanaman Padi Transgenik Balitbio terhadap Hama Penggerek Batang}},
    author = {I. S. Dewi and I. H. Somantri and D. Damayanti and A. Apriana and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {141-149},
    year = {2002},
    abstract = {The success in transgenic plants production occurred when plants express the inserted gene and interest new phenotype appears. Research team in RIFCB had succeeded in making putative rice transgenic plants containing cryIA(b) gene. The long-term objective of the research is to obtain transgenic rice resistance to stem borer. The observation in putative transgenic T1 Taipei-309 on sundep (dead-hearts) indicated that several plants were highly resistance (10 plants of T-1C and 1 plant of T-2H), resistance (3 plants of T-1C), and moderately resistance (2 plants of T-1C, 2 plants of T -1A, and 1 plant of T -2H). Others showed moderately susceptible (8 plants) and susceptible (5 plants), while 16 plants were highly susceptible to stem borer attack at vegetative phase. The observation on beluk (white-heads) indicated that several plants were highly resistance (15 plants of T-1C and 1 plant of T-2H). Only 1 plant of T-2H was moderately resistance. No plants can be identified as resistance. Several other plants were moderately susceptible (6 plants), susceptible (7 plants), and highly susceptible (26 plants). The research in bioassay of other putative transgenic rice was still on going.},
    keywords = {transgenic rice, bioassay, stemborer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_141-149_iswari.pdf}
    }
  14. Santoso, T. J., A. n. Apriana, A. D. Ambarwati, and I. H. Somantri. 2002. Evaluasi Diversitas Genetik Tanaman Padi Transgenik. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 150-157.
    [BibTeX] [Abstract] [PDF: Evaluasi Diversitas Genetik Tanaman Padi Transgenik ]
    The production of resistant varieties is economic and safe choise to control rice stem borer. The conventional rice improvement technique has several constraints due to the lack of high resistance rice varieties to be developed or crossed. Biotechnology approach or genetic engineering such as transformation technique can be developed to facilitate the conventional plant breeding program. The Bacillus thuringiensis (Bt) known produced the protein crystal that toxic to lepidopteran so that it can be used to control the rice stem borer. The production of transgenic plant involved several stages in molecular biology and celullar techniques, one of those is characterization or identification of the introduced gene into the plant genome. The characterization or identification aims to confirm the integrity of introduced gene and to decide the copy number in the plant genome as well as whether the gene function or not. Identification of the transforman can be conducted using some techniques such as PCR and Southern Blot analysis. The identification of cry1A(b) gene using PCR amplification result in 16 plants (from 38 plants) T1 cv. T309 that contains cry1A(b) gene. The Southern Blot analysis is now being conducted at the step of restriction digestion of the total genomic DNA. Therefore, the result of Southern Blot analysis cannot yet be reported.
    @article{Santoso02p150,
    title = {{Evaluasi Diversitas Genetik Tanaman Padi Transgenik}},
    author = {T. J. Santoso and A. n. Apriana and A. D. Ambarwati and I. H. Somantri},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {150-157},
    year = {2002},
    abstract = { The production of resistant varieties is economic and safe choise to control rice stem borer. The conventional rice improvement technique has several constraints due to the lack of high resistance rice varieties to be developed or crossed. Biotechnology approach or genetic engineering such as transformation technique can be developed to facilitate the conventional plant breeding program. The Bacillus thuringiensis (Bt) known produced the protein crystal that toxic to lepidopteran so that it can be used to control the rice stem borer. The production of transgenic plant involved several stages in molecular biology and celullar techniques, one of those is characterization or identification of the introduced gene into the plant genome. The characterization or identification aims to confirm the integrity of introduced gene and to decide the copy number in the plant genome as well as whether the gene function or not. Identification of the transforman can be conducted using some techniques such as PCR and Southern Blot analysis. The identification of cry1A(b) gene using PCR amplification result in 16 plants (from 38 plants) T1 cv. T309 that contains cry1A(b) gene. The Southern Blot analysis is now being conducted at the step of restriction digestion of the total genomic DNA. Therefore, the result of Southern Blot analysis cannot yet be reported.},
    keywords = { Transgenic, Bacillus thuringiensis, PCR},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_150-157_trijoko.pdf}
    }
  15. Sutrisno, Toto Hadiarto, Tri J. Santosa, Ifa Manzila, and Bahagiawati. 2002. Uji Stabilitas Ekspresi Gen CryIAc dalam Transforman Jagung R3 dan R4. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 158-163.
    [BibTeX] [Abstract] [PDF: Uji Stabilitas Ekspresi Gen CryIAc dalam Transforman Jagung R3 dan R4 ]
    In the fiscal year of 2000 we have obtained transformans R2 dan R3 that shows resistance to Asian corn borer, so it was expected that these transformans may positive to contain cryIAc crystal protein. In the fiscal year of 2001, cryIAc gene was tested its expression in the transformans R3 dan R4. Testing the expression was done by detecting the present of cryIAc crystal protein. The crystal protein was detected using Dot Blot-ELISA. In carrying out Dot Blot-ELISA, a series of activities was carried out, namely preparation cryIAc crystal protein as positive control, optimation of reaction between chemicals for ELISA with cryIAc crystal protein from Bt isolat (as positive control), and reaction of chemicals for ELISA with protein from maize transformans. Bt isolat tested that was expected to contain cryIAc crystal protein were amplified with primers specific for cryIAc gene. The results of amplification indicate that four isolates contain a DNA fragmen with the size the same with cryIAc DNA fragments. The four isolates were multiplied in the Lurient Broth Media and cryIAc crystal protein was isolated from those culture and use as a positive control. Dot Blot-ELISA for crystal protein derived from the four isolates showed positive reaction. Dot Blot-ELISA using protein extracted from maize transformants showed negative reaction.
    @article{Sutrisno02p158,
    title = {{Uji Stabilitas Ekspresi Gen CryIAc dalam Transforman Jagung R3 dan R4}},
    author = {Sutrisno and Toto Hadiarto and Tri J. Santosa and Ifa Manzila and Bahagiawati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {158-163},
    year = {2002},
    abstract = {In the fiscal year of 2000 we have obtained transformans R2 dan R3 that shows resistance to Asian corn borer, so it was expected that these transformans may positive to contain cryIAc crystal protein. In the fiscal year of 2001, cryIAc gene was tested its expression in the transformans R3 dan R4. Testing the expression was done by detecting the present of cryIAc crystal protein. The crystal protein was detected using Dot Blot-ELISA. In carrying out Dot Blot-ELISA, a series of activities was carried out, namely preparation cryIAc crystal protein as positive control, optimation of reaction between chemicals for ELISA with cryIAc crystal protein from Bt isolat (as positive control), and reaction of chemicals for ELISA with protein from maize transformans. Bt isolat tested that was expected to contain cryIAc crystal protein were amplified with primers specific for cryIAc gene. The results of amplification indicate that four isolates contain a DNA fragmen with the size the same with cryIAc DNA fragments. The four isolates were multiplied in the Lurient Broth Media and cryIAc crystal protein was isolated from those culture and use as a positive control. Dot Blot-ELISA for crystal protein derived from the four isolates showed positive reaction. Dot Blot-ELISA using protein extracted from maize transformants showed negative reaction.},
    keywords = {expression cryiac genes, dot blot-elisa},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_158-163_sutrisno.pdf}
    }
  16. Hadiarto, T., Sutrisno, and T. J. Santoso. 2002. Uji Stabilitas Integrasi Gen CryIAc dalam Transforman Jagung R3 dan R4. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 164-169.
    [BibTeX] [Abstract] [PDF: Uji Stabilitas Integrasi Gen CryIAc dalam Transforman Jagung R3 dan R4 ]
    The genetically modification of maize to produce the plant resistant to stem borer has been carried out. Cry gene whose product has been known to be toxic to stem borer was introduced into the plants using the method of Agrobacterium or particle bombardment. To analyze whether or not the plants contain the introduced gene, molecular investigation was performed. 90 samples were examined for the presence of the cry gene by means of PCR. The use of PCR technique is to ease and fasten the analysis of large number of samples. Two pairs of specific primers were used to amplify parts of cry gene. Primers cryIAc will produce 490 bp fragment whereas primers cryIAb/Ac will amplify cry gene to produce fragment of 450 bp. It is found that none of the fragments have been amplified under the optimum PCR conditions. This indicates the absence of cry gene which might be due to its instability in the plant cells. As a result, the objective to produce maize line that is resistant to stem borer has not yet been achieved.
    @article{Hadiarto02p164,
    title = {{Uji Stabilitas Integrasi Gen CryIAc dalam Transforman Jagung R3 dan R4}},
    author = {T. Hadiarto and Sutrisno and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {164-169},
    year = {2002},
    abstract = { The genetically modification of maize to produce the plant resistant to stem borer has been carried out. Cry gene whose product has been known to be toxic to stem borer was introduced into the plants using the method of Agrobacterium or particle bombardment. To analyze whether or not the plants contain the introduced gene, molecular investigation was performed. 90 samples were examined for the presence of the cry gene by means of PCR. The use of PCR technique is to ease and fasten the analysis of large number of samples. Two pairs of specific primers were used to amplify parts of cry gene. Primers cryIAc will produce 490 bp fragment whereas primers cryIAb/Ac will amplify cry gene to produce fragment of 450 bp. It is found that none of the fragments have been amplified under the optimum PCR conditions. This indicates the absence of cry gene which might be due to its instability in the plant cells. As a result, the objective to produce maize line that is resistant to stem borer has not yet been achieved.},
    keywords = {Agrobacterium, particle bombardment, PCR},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_164-169_totohadiarto.pdf}
    }
  17. Sutrisno, Budihardjo Soegiarto, and Diani Damayanti. 2002. Uji Keefektifan Transforman Jagung R3 dan R4 terhadap Penggerek Batang Jagung. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 170-176.
    [BibTeX] [Abstract] [PDF: Uji Keefektifan Transforman Jagung R3 dan R4 terhadap Penggerek Batang Jagung ]
    Testing the efficacy of maize transformans of R3 and R4 against Asian corn borer was conducted in the Biosafety Containment of Research Institute for Food Crop Biotechnology. The aim of testing was to evaluate the response of maize transformans against Asian corn borer. One hundred thirty five putative transgenic plants (Antasena cryIAc) was tested their response against Asian corn borer. Out of 135 putative transgenic plants, 22 plants showed highly resistance, 41 plants resistance, and 72 plants susceptible. The mortality of neonate larvae expose to transgenic plants (66%) was higher than those in the non-transgenic plants (16%). The size of larva of Asian corn borer feed on transgenic plants (3.7 mm) was shorter than those feed on non-transgenic plants (5.3 mm).
    @article{Sutrisno02p170,
    title = {{Uji Keefektifan Transforman Jagung R3 dan R4 terhadap Penggerek Batang Jagung}},
    author = {Sutrisno and Budihardjo Soegiarto and Diani Damayanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {170-176},
    year = {2002},
    abstract = {Testing the efficacy of maize transformans of R3 and R4 against Asian corn borer was conducted in the Biosafety Containment of Research Institute for Food Crop Biotechnology. The aim of testing was to evaluate the response of maize transformans against Asian corn borer. One hundred thirty five putative transgenic plants (Antasena cryIAc) was tested their response against Asian corn borer. Out of 135 putative transgenic plants, 22 plants showed highly resistance, 41 plants resistance, and 72 plants susceptible. The mortality of neonate larvae expose to transgenic plants (66%) was higher than those in the non-transgenic plants (16%). The size of larva of Asian corn borer feed on transgenic plants (3.7 mm) was shorter than those feed on non-transgenic plants (5.3 mm).},
    keywords = {testing the efficacy, asian corn borer, maize transgenic},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_170-176_sutrisno.pdf}
    }
  18. Prasetiyono, J., Tasliah, and S. Moeljopawiro. 2002. Survei Primer Mikrosatelit dan Isolasi DNA Tanaman F2 (Dupa x ITA131). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 177-184.
    [BibTeX] [Abstract] [PDF: Survei Primer Mikrosatelit dan Isolasi DNA Tanaman F2 (Dupa x ITA131) ]
    Acid soil in Indonesia is about 27.5 million hectares (29.7% of Indonesian mainland). Recent development in molecular biology can be used to identify molecular markers of trait related to aluminum toxicity tolerance. Microsatellite markers is effective, economical, and practical. In order to identify microsatellite markers, it will be mapped alleles that linked to aluminum toxicity tolerance on Indonesian cultivar. Map will be developed on 190 F2 generation of crossing between Dupa cultivar (Indonesia, higly tolerant) and ITA131 (Africa, very sensitive). Phenotypic data will be obtained from growing F2 and F3 under Al stress versus non stress condition. 243 microsatellite markers will be apllied. Mapmaker/QTL program will be used for this mapping purpose. Phenotypic test on F2 was finished at 2000. At 2001, It have been finished primers survey and resulted 110 pholimorphic primers. 70 polimorphic primers will be applied to 190 F2 (Dupa x ITA131). DNA of F2 have been isolated and ready to be amplified using selected primers.
    @article{Prasetiyono02p177,
    title = {{Survei Primer Mikrosatelit dan Isolasi DNA Tanaman F2 (Dupa x ITA131)}},
    author = {J. Prasetiyono and Tasliah and S. Moeljopawiro},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {177-184},
    year = {2002},
    abstract = {Acid soil in Indonesia is about 27.5 million hectares (29.7% of Indonesian mainland). Recent development in molecular biology can be used to identify molecular markers of trait related to aluminum toxicity tolerance. Microsatellite markers is effective, economical, and practical. In order to identify microsatellite markers, it will be mapped alleles that linked to aluminum toxicity tolerance on Indonesian cultivar. Map will be developed on 190 F2 generation of crossing between Dupa cultivar (Indonesia, higly tolerant) and ITA131 (Africa, very sensitive). Phenotypic data will be obtained from growing F2 and F3 under Al stress versus non stress condition. 243 microsatellite markers will be apllied. Mapmaker/QTL program will be used for this mapping purpose. Phenotypic test on F2 was finished at 2000. At 2001, It have been finished primers survey and resulted 110 pholimorphic primers. 70 polimorphic primers will be applied to 190 F2 (Dupa x ITA131). DNA of F2 have been isolated and ready to be amplified using selected primers.},
    keywords = {aluminum toxicity, microsatellites marker, dupa, ita131},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_177-184_jokoprasetiyono.pdf}
    }
  19. Budiarti, S. G., Sutoro, Hadiatmi, and H. Purwanti. 2002. Pembentukan dan Evaluasi Inbrida Jagung Tahan Penyakit Bulai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 193-198.
    [BibTeX] [Abstract] [PDF: Pembentukan dan Evaluasi Inbrida Jagung Tahan Penyakit Bulai ]
    Hybrid variety which resistant to downey mildew is one of method to increase maize production in Indonesia. Pool gen 4 and pool gen 5, and germplasm collection can used for looking for breeding material with specific purpose including resistant to downey mildew. The experiment is continued experiment in 2000. Selfing from 60 inbred, found 200 ears (new inbred) that were tested to downey mildew at Cikeumeuh Installation, RIFCB in the WS 2001. A randomized complete block design with twice replicated was used to test two hundred inbred. Antasena (susceptible check) was planted as a border for inoculums source of downey mildew. Inbred tested were planted after Antasena have attached by downey mildew + 70% at 21 days after planting. At four days after planting, inbreds were inoculated by spora suspension. Each inbred was planted with spacing 70 cm x 20 cm (25 plants for a row). The check varieties, namely Arjuna TB (resistant check) and Antasena (susceptible check) were planted in each 25 inbreds tested. The result showed that selfing for healthy plants in downey mildew nursery can the improve resistance to downey mildew. From 200 inbreds that were tested to downey mildew, found 34 inbreds highly resistant and 31 inbreds resistant to downey mildew. Resistant and highly resistant inbreds to downey mildew conserved as resistant gen source.
    @article{Budiarti02p193,
    title = {{Pembentukan dan Evaluasi Inbrida Jagung Tahan Penyakit Bulai}},
    author = {S. G. Budiarti and Sutoro and Hadiatmi and H. Purwanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {193-198},
    year = {2002},
    abstract = {Hybrid variety which resistant to downey mildew is one of method to increase maize production in Indonesia. Pool gen 4 and pool gen 5, and germplasm collection can used for looking for breeding material with specific purpose including resistant to downey mildew. The experiment is continued experiment in 2000. Selfing from 60 inbred, found 200 ears (new inbred) that were tested to downey mildew at Cikeumeuh Installation, RIFCB in the WS 2001. A randomized complete block design with twice replicated was used to test two hundred inbred. Antasena (susceptible check) was planted as a border for inoculums source of downey mildew. Inbred tested were planted after Antasena have attached by downey mildew + 70% at 21 days after planting. At four days after planting, inbreds were inoculated by spora suspension. Each inbred was planted with spacing 70 cm x 20 cm (25 plants for a row). The check varieties, namely Arjuna TB (resistant check) and Antasena (susceptible check) were planted in each 25 inbreds tested. The result showed that selfing for healthy plants in downey mildew nursery can the improve resistance to downey mildew. From 200 inbreds that were tested to downey mildew, found 34 inbreds highly resistant and 31 inbreds resistant to downey mildew. Resistant and highly resistant inbreds to downey mildew conserved as resistant gen source.},
    keywords = {inbred, downey mildew, maize},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_193-198_srigajatri.pdf}
    }
  20. Abdullah, B., Suwarno, B. Kustianto, and H. Siregar. 2002. Pembentukan Galur Padi Sawah Tipe Baru. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 199-207.
    [BibTeX] [Abstract] [PDF: Pembentukan Galur Padi Sawah Tipe Baru ]
    New plant type of rice (NPT) is a new architecture of rice having characteristics of sturdy stem, few but all productive tillers, long panicle with 200-300 grains, high percentage filled grain (>90%), thick, ereck and dark green leaves, good root system, medium-short plant height (100-110 cm), early maturity (110-120 days). This NPT is predicted to have yield potential 20% higher than that of existing improved varieties. Therefore, efforts to develop NPT varieties is urgent to increase national rice production and meet rice demand. Numbers of rice lines have been produced through conventional breeding as well as anther culture. Two thousand nine hundred and fifteen lines were evaluated for higher yield potential in pedigree nurseries. Of these, 137 lines had been homogenous and had desirable characteristics for high yield potential. These lines will be further evaluated in observation yield trial. Other 417 lines had desirable characteristics but were still heterogenous, therefore, 3-6 best plants of each lines were selected for reevaluated in pedigree nursery. Out of 558 advanced lines of observation yield trials, 153 lines had yield potential of >9 t/ha. Sixteen lines had yield potential of 13-17.9 t/ha, 10-40% higher than of IR64 (12.8 t/ha).
    @article{Abdullah02p199,
    title = {{Pembentukan Galur Padi Sawah Tipe Baru}},
    author = {B. Abdullah and Suwarno and B. Kustianto and H. Siregar},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {199-207},
    year = {2002},
    abstract = {New plant type of rice (NPT) is a new architecture of rice having characteristics of sturdy stem, few but all productive tillers, long panicle with 200-300 grains, high percentage filled grain (>90%), thick, ereck and dark green leaves, good root system, medium-short plant height (100-110 cm), early maturity (110-120 days). This NPT is predicted to have yield potential 20% higher than that of existing improved varieties. Therefore, efforts to develop NPT varieties is urgent to increase national rice production and meet rice demand. Numbers of rice lines have been produced through conventional breeding as well as anther culture. Two thousand nine hundred and fifteen lines were evaluated for higher yield potential in pedigree nurseries. Of these, 137 lines had been homogenous and had desirable characteristics for high yield potential. These lines will be further evaluated in observation yield trial. Other 417 lines had desirable characteristics but were still heterogenous, therefore, 3-6 best plants of each lines were selected for reevaluated in pedigree nursery. Out of 558 advanced lines of observation yield trials, 153 lines had yield potential of >9 t/ha. Sixteen lines had yield potential of 13-17.9 t/ha, 10-40% higher than of IR64 (12.8 t/ha).},
    keywords = {new plant type, rice, high yield potential},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_199-207_buangabdullah.pdf}
    }
  21. Somantri, I. H., A. D. Ambarwati, and A. Apriana. 2002. Perbaikan Varietas Padi melalui Kultur Anter. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 208-214.
    [BibTeX] [Abstract] [PDF: Perbaikan Varietas Padi melalui Kultur Anter ]
    Anther culture has been used in rice improvement programs. It is well known that the obvious advantage of anther culture is the quick route to achieve homozygotes, however one of the problems arise in anther culture is that the production of albino plants and the dependence of culturability on genotypes. The result showed that each genotype has the different response to anther culture, the addition of putresine to callus induction and regeneration medium could increase the percentage of plant regeneration, both green plants and albino plants, the genotype of IR66160-121-4-53/Memberamo and IR66738-18-2-2/Cabacu gave the higher percentage of callus induction, total plants production and green plants production than Turanta/IR64//IR64///Memberamo/ IR59552. Among three genotypes used in anther culture, IR66738-18-2-2/ Cabacu had the highest response to callus induction and total plants production (green plants and albino plants). The experiment should be continued to select the plants produced from anther culture.
    @article{Somantri02p208,
    title = {{Perbaikan Varietas Padi melalui Kultur Anter}},
    author = {I. H. Somantri and A. D. Ambarwati and A. Apriana},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {208-214},
    year = {2002},
    abstract = {Anther culture has been used in rice improvement programs. It is well known that the obvious advantage of anther culture is the quick route to achieve homozygotes, however one of the problems arise in anther culture is that the production of albino plants and the dependence of culturability on genotypes. The result showed that each genotype has the different response to anther culture, the addition of putresine to callus induction and regeneration medium could increase the percentage of plant regeneration, both green plants and albino plants, the genotype of IR66160-121-4-53/Memberamo and IR66738-18-2-2/Cabacu gave the higher percentage of callus induction, total plants production and green plants production than Turanta/IR64//IR64///Memberamo/ IR59552. Among three genotypes used in anther culture, IR66738-18-2-2/ Cabacu had the highest response to callus induction and total plants production (green plants and albino plants). The experiment should be continued to select the plants produced from anther culture.},
    keywords = {rice, anther culture, genetic},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_208-214_idahanarida.pdf}
    }
  22. Rusyadi, Y., N. Sunarlim, I. Mariska, and Murtado. 2002. Multiplikasi Tunas Tanaman Melinjo melalui Kultur In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 215-221.
    [BibTeX] [Abstract] [PDF: Multiplikasi Tunas Tanaman Melinjo melalui Kultur In Vitro ]
    Multiplication of Gnetum gnemon using in vitro culture is expected to obtain the plants faster and the factor of multiplication is high. The explants (result of the experiment of 2000) was subcultured to 2 different basal media (WPM and Anderson) with 4 rates of thidiazuron (0.1, 0.3, and 0.5 mg/l) and 2 rates of BA (0.5 and 1.0 mg/l). Result of the experiment showed that basal media of Anderson was better than WPM. Formulation of media of Anderson + BA 0.5 mg/l + thidiazuron 0.1 mg/l was more effective than those other treatments. The highest number of shoots and leaves was obtained from this treatment.
    @article{Rusyadi02p215,
    title = {{Multiplikasi Tunas Tanaman Melinjo melalui Kultur In Vitro}},
    author = {Y. Rusyadi and N. Sunarlim and I. Mariska and Murtado},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {215-221},
    year = {2002},
    abstract = {Multiplication of Gnetum gnemon using in vitro culture is expected to obtain the plants faster and the factor of multiplication is high. The explants (result of the experiment of 2000) was subcultured to 2 different basal media (WPM and Anderson) with 4 rates of thidiazuron (0.1, 0.3, and 0.5 mg/l) and 2 rates of BA (0.5 and 1.0 mg/l). Result of the experiment showed that basal media of Anderson was better than WPM. Formulation of media of Anderson + BA 0.5 mg/l + thidiazuron 0.1 mg/l was more effective than those other treatments. The highest number of shoots and leaves was obtained from this treatment.},
    keywords = {gnetum gnemon, in vitro culture, multiplication},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_215-221_yadirusyadi.pdf}
    }
  23. Supriati, Y., W. H. Adil, D. Sukmadjaja, and I. Mariska. 2002. Peningkatan Multiplikasi Tunas dan Induksi Akar Tanaman Iles-iles melalui Kultur In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 222-229.
    [BibTeX] [Abstract] [PDF: Peningkatan Multiplikasi Tunas dan Induksi Akar Tanaman Iles-iles melalui Kultur In Vitro ]
    Iles-iles (Amorpophalus spp.) is one of the root crops which is needed to develop due to its potentially as a commodity export. Japan and Taiwan need iles-iles for flour industry which is known as conjac flour. This flour contains of glucomannan that ordinary used as a healthy food which is useful for a dietary fiber. There are a lot of iles-iles varieties in Indonesia, but unfortunately it never been cultivated. It is because of the information of iles-iles functions for human being is still lack, whereas in fact iles-iles is also used for medicine and paper industry besides for food industry. In relation with iles-iles production and development, we need the information of culture technology and the availability of seedlings. Long dormancy time of this plant during its life cycle resulted time consuming in the generative multiplication of this plant. Therefore, an alternative technology in plant multiplication was needed. Tissue culture technique which was useful on many other ornamental plant maybe favorable for micro propagation of iles-iles. In the previous research (2000) the formula of shoot multiplication of iles-iles was found. By using the basal MS media with 3 mg/l of kinetin shoot initiation was stimulated, nevertheless the number of shoot was only one per explants. In the next research (2001), for increasing the number of shoot, two rates of benzyladeninpurin (BAP) (3 and 5 mg/l) was applied in combination with three rates of kinetin (0, 0.5, and 1 mg/l) while for stimulating the root induction, 3 rates of IBA (0, 0.5, and 1.0 mg/l) was applied in combination with two rates of basal MS media (full and half formula). Both of the experiments were arranged in completely randomized design with 10 and 20 replications respectively. The result showed that the use of kinetin which was applied in combination with BAP and or IBA, resulted negative effect on shoot proliferation of iles-iles. The best formula to increase shoot multiplication was MS media + BAP 3 mg/l and Kinetin 5 mg/l. IBA had the negative effect on root induction and proliferation of iles-iles.
    @article{Supriati02p222,
    title = {{Peningkatan Multiplikasi Tunas dan Induksi Akar Tanaman Iles-iles melalui Kultur In Vitro}},
    author = {Y. Supriati and W. H. Adil and D. Sukmadjaja and I. Mariska},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {222-229},
    volume = {0},
    year = {2002},
    abstract = {Iles-iles (Amorpophalus spp.) is one of the root crops which is needed to develop due to its potentially as a commodity export. Japan and Taiwan need iles-iles for flour industry which is known as conjac flour. This flour contains of glucomannan that ordinary used as a healthy food which is useful for a dietary fiber. There are a lot of iles-iles varieties in Indonesia, but unfortunately it never been cultivated. It is because of the information of iles-iles functions for human being is still lack, whereas in fact iles-iles is also used for medicine and paper industry besides for food industry. In relation with iles-iles production and development, we need the information of culture technology and the availability of seedlings. Long dormancy time of this plant during its life cycle resulted time consuming in the generative multiplication of this plant. Therefore, an alternative technology in plant multiplication was needed. Tissue culture technique which was useful on many other ornamental plant maybe favorable for micro propagation of iles-iles. In the previous research (2000) the formula of shoot multiplication of iles-iles was found. By using the basal MS media with 3 mg/l of kinetin shoot initiation was stimulated, nevertheless the number of shoot was only one per explants. In the next research (2001), for increasing the number of shoot, two rates of benzyladeninpurin (BAP) (3 and 5 mg/l) was applied in combination with three rates of kinetin (0, 0.5, and 1 mg/l) while for stimulating the root induction, 3 rates of IBA (0, 0.5, and 1.0 mg/l) was applied in combination with two rates of basal MS media (full and half formula). Both of the experiments were arranged in completely randomized design with 10 and 20 replications respectively. The result showed that the use of kinetin which was applied in combination with BAP and or IBA, resulted negative effect on shoot proliferation of iles-iles. The best formula to increase shoot multiplication was MS media + BAP 3 mg/l and Kinetin 5 mg/l. IBA had the negative effect on root induction and proliferation of iles-iles.},
    keywords = {Amorpophalus spp., in vitro culture, multiplication, root induction},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_222-229_yatisupriyati.pdf}
    }
  24. Tambunan, I. R., N. Sunarlim, I. Mariska, and M. Kosmiatin. 2002. Kriopreservasi Ubi Jalar dan Optimasi Regenerasi Ubi Kayu dengan Penekanan Pelayuan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 230-243.
    [BibTeX] [Abstract] [PDF: Kriopreservasi Ubi Jalar dan Optimasi Regenerasi Ubi Kayu dengan Penekanan Pelayuan ]
    Conservation with cryopreservation is an in vitro culture technique with highly potential for germplasm long conservation. The experiments consisted of 2 activities, the first activity was cryopreservation for sweet potato and cassava and the second activity was the regeneration of cassava. For the sweet potato cyopreservation experiment, combination of base media (MS and modification MS) and incubation temperatures (cold and room temperatures) was applied for pregrowth and preculture treatments. For preculture treatment, ethylene glycol or DMSO at the rates of 1.5 and 3% was applied to the base media. Exposure in the cryoprotectant was done gradually. Thawing was using rapid thawing. For cassava cryopreservation experiment, preculture treatment was done in the base media supplemented with sucrose at the rate of 0.3 M for 2-5 days. In the experiment of cassava regeneration, the base media (MS and DKW) was combined with Kinetin or BA and applied with glutamin. Result of the experiments showed that the best treatment for pregrowth and preculture of sweet potato was MS medium with room temperature. Using ethylene glycol was better than DMSO for preculture treatment. Explants of sweet potato survived and regenerated well after exposure to PVS2 although the percentage was lower than before the exposure. After the exposure to liquid nitrogen, the explants could not survive. The visual observation to cassava preculture treatment, the explants was still green and vigor. Base media of MS and DKW gave the same affect on the cassava growth and reduced of senesence. The best media for cassava multiplication was DKW with application of BA 3-10 mg/l, glutamin 100 mg/l, and NAA 0.001 mg/l.
    @article{Tambunan02p230,
    title = {{Kriopreservasi Ubi Jalar dan Optimasi Regenerasi Ubi Kayu dengan Penekanan Pelayuan}},
    author = {I. R. Tambunan and N. Sunarlim and I. Mariska and M. Kosmiatin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {230-243},
    year = {2002},
    abstract = {Conservation with cryopreservation is an in vitro culture technique with highly potential for germplasm long conservation. The experiments consisted of 2 activities, the first activity was cryopreservation for sweet potato and cassava and the second activity was the regeneration of cassava. For the sweet potato cyopreservation experiment, combination of base media (MS and modification MS) and incubation temperatures (cold and room temperatures) was applied for pregrowth and preculture treatments. For preculture treatment, ethylene glycol or DMSO at the rates of 1.5 and 3% was applied to the base media. Exposure in the cryoprotectant was done gradually. Thawing was using rapid thawing. For cassava cryopreservation experiment, preculture treatment was done in the base media supplemented with sucrose at the rate of 0.3 M for 2-5 days. In the experiment of cassava regeneration, the base media (MS and DKW) was combined with Kinetin or BA and applied with glutamin. Result of the experiments showed that the best treatment for pregrowth and preculture of sweet potato was MS medium with room temperature. Using ethylene glycol was better than DMSO for preculture treatment. Explants of sweet potato survived and regenerated well after exposure to PVS2 although the percentage was lower than before the exposure. After the exposure to liquid nitrogen, the explants could not survive. The visual observation to cassava preculture treatment, the explants was still green and vigor. Base media of MS and DKW gave the same affect on the cassava growth and reduced of senesence. The best media for cassava multiplication was DKW with application of BA 3-10 mg/l, glutamin 100 mg/l, and NAA 0.001 mg/l.},
    keywords = {Cryopreservation, Ipomea batatas, Manihot utilissima},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_230-243_ikaroostika.pdf}
    }
  25. Sunarlim, N., N. Dewi, and I. R. Tambunan. 2002. Penyimpanan Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas secara In Vitro dengan Pertumbuhan Minimal. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 244-255.
    [BibTeX] [Abstract] [PDF: Penyimpanan Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas secara In Vitro dengan Pertumbuhan Minimal ]
    Conservation of tuber crops in in vitro culture can decrease the areas, man power and budget, also decrease the possibility to loss of genetic germplasm due to environmental stress. In vitro culture with minimum growth can conserve them in longer time. The purpose of this experiment was to conserve 50 accessions of sweet potato, 20 accessions of cassava, and 10 accessions of taro. The sterilization was done by using alcohol 70%, clorox 30 and 20%. The explants which were steril then multiplied and conserved in conservation media. For taro there is one trial on media conservation using paclobutrazol at the level of 0, 1, 3, and 5 mg/l. Results of the experiments showed that out of 50 sweet potato accessions tried, 3 accessions were steril and some of them were already 6 conserved in conservation media. For cassava, out of 20 accessions tried, only 5 accessions were steril and now being multiplied. Sterilization of 10 accessions of taro resulted in 5 a ccessions steril and used for paclobutrazol trial. Result of paclobutrazol trial showed that increased consentration of paclobutrazol, the explants became shorter and number shoot was fewer.
    @article{Sunarlim02p244,
    title = {{Penyimpanan Plasma Nutfah Ubi Jalar, Ubi Kayu, dan Talas secara In Vitro dengan Pertumbuhan Minimal}},
    author = {N. Sunarlim and N. Dewi and I. R. Tambunan},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {244-255},
    year = {2002},
    abstract = {Conservation of tuber crops in in vitro culture can decrease the areas, man power and budget, also decrease the possibility to loss of genetic germplasm due to environmental stress. In vitro culture with minimum growth can conserve them in longer time. The purpose of this experiment was to conserve 50 accessions of sweet potato, 20 accessions of cassava, and 10 accessions of taro. The sterilization was done by using alcohol 70%, clorox 30 and 20%. The explants which were steril then multiplied and conserved in conservation media. For taro there is one trial on media conservation using paclobutrazol at the level of 0, 1, 3, and 5 mg/l. Results of the experiments showed that out of 50 sweet potato accessions tried, 3 accessions were steril and some of them were already 6 conserved in conservation media. For cassava, out of 20 accessions tried, only 5 accessions were steril and now being multiplied. Sterilization of 10 accessions of taro resulted in 5 a ccessions steril and used for paclobutrazol trial. Result of paclobutrazol trial showed that increased consentration of paclobutrazol, the explants became shorter and number shoot was fewer.},
    keywords = {conservation, in vitro culture, ipomoea batatas, manihot utilisima, colocasia esculenta},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_244-255_novianti.pdf}
    }
  26. Lestari, E. G., I. Mariska, and Mujiman. 2002. Studi Regenerasi pada Beberapa Varietas Padi Indica. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 256-264.
    [BibTeX] [Abstract] [PDF: Studi Regenerasi pada Beberapa Varietas Padi Indica ]
    Due to its position as one of the main world staple food, rice need is growing along with the increasing numbers of world population. To fulfil the requirements, high-yielding varieties which is tolerant to certain should be developed. Due to the limited rice-field area, the alternative is, among others, by developing the varieties tolerant to stress such as acid soil, salinity and dryness. To produce new varieties tolerant to the particular environment, both conventional and biotechnological method could be applied. Biotechnological method could be carried out with the crossing and selection from the available genotype and by in vitro culture both through somaclonal variation and the in vitro selection. This research is aimed at obtaining the optimum formulation media to shoot regeneration at several Indica variety. The plant used is the zygotic embryo from Cisadane, Bengawan Solo, and Gajah Mungkur varieties. The research consists of two steps, namely callus induction and shoot regeneration. The basic media is Murashige and Skoog (MS) + vitamin group B + gel rite 2.5 g/l and sucrose 3%. The added growth regulator is auksin (2.4-D, NAA, and IAA) and sitokinin (BA, kinetin, and zeatin). The result shows that the most appropriate media for callus inductin from the three varieties is MS + 2.4-D 2 mg/l + casein hidrolisat 2 g/l. Media for regeration at Cisadane variety is BA 5 mg/l + IAA 0.1 mg/l + zeatin 0.1 mg/l. For multiplication, the addition of BA 0.5 mg/l after 3 weeks could produce shoot as many as 1.8. Bengawan Solo variety is BA 3 mg/l + NAA 0.1 mg/l + kinetin 1 mg/l. At Gajah Mungkur variety, the most optimum regeneration is obtained from the media BA 3 mg/l + IAA 0.1 mg/l + zeatin 0.2 mg/l.
    @article{Lestari02p256,
    title = {{Studi Regenerasi pada Beberapa Varietas Padi Indica}},
    author = {E. G. Lestari and I. Mariska and Mujiman},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {256-264},
    year = {2002},
    abstract = {Due to its position as one of the main world staple food, rice need is growing along with the increasing numbers of world population. To fulfil the requirements, high-yielding varieties which is tolerant to certain should be developed. Due to the limited rice-field area, the alternative is, among others, by developing the varieties tolerant to stress such as acid soil, salinity and dryness. To produce new varieties tolerant to the particular environment, both conventional and biotechnological method could be applied. Biotechnological method could be carried out with the crossing and selection from the available genotype and by in vitro culture both through somaclonal variation and the in vitro selection. This research is aimed at obtaining the optimum formulation media to shoot regeneration at several Indica variety. The plant used is the zygotic embryo from Cisadane, Bengawan Solo, and Gajah Mungkur varieties. The research consists of two steps, namely callus induction and shoot regeneration. The basic media is Murashige and Skoog (MS) + vitamin group B + gel rite 2.5 g/l and sucrose 3%. The added growth regulator is auksin (2.4-D, NAA, and IAA) and sitokinin (BA, kinetin, and zeatin). The result shows that the most appropriate media for callus inductin from the three varieties is MS + 2.4-D 2 mg/l + casein hidrolisat 2 g/l. Media for regeration at Cisadane variety is BA 5 mg/l + IAA 0.1 mg/l + zeatin 0.1 mg/l. For multiplication, the addition of BA 0.5 mg/l after 3 weeks could produce shoot as many as 1.8. Bengawan Solo variety is BA 3 mg/l + NAA 0.1 mg/l + kinetin 1 mg/l. At Gajah Mungkur variety, the most optimum regeneration is obtained from the media BA 3 mg/l + IAA 0.1 mg/l + zeatin 0.2 mg/l.},
    keywords = {Indica rice, regeneration, Cisadane, Gajah Mungkur, Bengawan Solo},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_256-264_endang.pdf}
    }
  27. Purnamaningsih, R., I. Mariska, and A. Husni. 2002. Produksi Kalus Embriogenik dan Regenerasinya Setelah Seleksi In Vitro dengan Al dan pH Rendah pada Tanaman Padi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 265-275.
    [BibTeX] [Abstract] [PDF: Produksi Kalus Embriogenik dan Regenerasinya Setelah Seleksi In Vitro dengan Al dan pH Rendah pada Tanaman Padi ]
    One alternative to increase national productivity of rice is using marginal soil (acid soil). The problem in that soil is invironmental stress such as high soil acidity, low of mineral solubility (N, P, K, Ca, Mg, and Mo), and Al and Mn toxicity. Finding the variety which is tolerant to environmental stress could solve the problem. Unfortunately the number of variety is limited. The aim of the experiment is to get variety of rice that tolerant to the environmental stress. The treatment consisted of 2 varieties (T-309 and Rojolele) and 6 levels of aluminum concentration (0, 100, 200, 300, 400, and 500 ppm). Selection was done in three stages: regeneration, embryo, and callus. Completely randomized design with factorial arrangement was used in this experiment. Result of the experiment showed that the varieties had the same respon in all media. In the treatment of MS + 2.4-D 2 mg/l + casein hidrolysat 3 g/l produced more noduled or bud formation than others. After in vitro selection, explant could regenerate in all Al concentration, except in Al 500 ppm. Selection on stage of callus, regeneration and embryo had the same result. Increased of Al concentration resulting decreased of regeneration ability. In the regeneration and embryo stage, regeneration of T-309 was not different from Rojolele. In the callus stage regeneration of T-309 (47.76%) was higher than Rojolele (15.38%).
    @article{Purnamaningsih02p265,
    title = {{Produksi Kalus Embriogenik dan Regenerasinya Setelah Seleksi In Vitro dengan Al dan pH Rendah pada Tanaman Padi}},
    author = {R. Purnamaningsih and I. Mariska and A. Husni},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {265-275},
    year = {2002},
    abstract = {One alternative to increase national productivity of rice is using marginal soil (acid soil). The problem in that soil is invironmental stress such as high soil acidity, low of mineral solubility (N, P, K, Ca, Mg, and Mo), and Al and Mn toxicity. Finding the variety which is tolerant to environmental stress could solve the problem. Unfortunately the number of variety is limited. The aim of the experiment is to get variety of rice that tolerant to the environmental stress. The treatment consisted of 2 varieties (T-309 and Rojolele) and 6 levels of aluminum concentration (0, 100, 200, 300, 400, and 500 ppm). Selection was done in three stages: regeneration, embryo, and callus. Completely randomized design with factorial arrangement was used in this experiment. Result of the experiment showed that the varieties had the same respon in all media. In the treatment of MS + 2.4-D 2 mg/l + casein hidrolysat 3 g/l produced more noduled or bud formation than others. After in vitro selection, explant could regenerate in all Al concentration, except in Al 500 ppm. Selection on stage of callus, regeneration and embryo had the same result. Increased of Al concentration resulting decreased of regeneration ability. In the regeneration and embryo stage, regeneration of T-309 was not different from Rojolele. In the callus stage regeneration of T-309 (47.76%) was higher than Rojolele (15.38%).},
    keywords = {Oryza sativa, in vitro selection, acid soil, aluminum},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_265-275_ragapadmi.pdf}
    }
  28. Sukmadjaja, D., I. Mariska, E. G. Lestari, M. Kosmiatin, M. Tombe, and Hobir. 2002. Seleksi Silang Tunas Abaka dengan Asam Fusarat atau Filtrat F. Oxysporum dan Regenerasinya Membentuk Planlet. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 276-288.
    [BibTeX] [Abstract] [PDF: Seleksi Silang Tunas Abaka dengan Asam Fusarat atau Filtrat F. Oxysporum dan Regenerasinya Membentuk Planlet ]
    Gamma-ray irradiation as a mutagen was used to increased somaclonal variation on abaca in vitro culture. Abaca calli were exposed to 0, 0.5, 1.0, 1.5, 2.0, and 3.0 Krad of gamma rays. The experiment results showed that callus growth capacity decreased with increasing dosages of gamma rays irradiation. Callus capacity to regenerate stopped at 3.0 Krad of irradiation dose. The LD50 was found between 1.0-1.5 Krad of irradiaton dose. To obtain new clones of resistant abaca to F. oxysporum, in vitro selection was treated by using two kind selection components with different concentrations: pure toxin of fusaric acid (1, 15, 30, 45, 60, and 75 ppm) and F. oxysporum filtrate (10, 30, and 50%). Selection was carried out in two stages. Concentration of selection components in stages II increased one level to concentration in stage I. The LD50 of selection in stage I was found at 1.0 Krad of irradiation dose with 15 ppm of fusaric acid as selection component. At stage I selection showed that a higher irradiation dose and selection components decreases shoot regeneration capacity. The highest number and height of shoot were observed from the 1.0 Krad of irradiation dose at 0 ppm followed by 1.0 Krad of irradiation dose with 15 ppm of fusaric acid as selection component. The similar results were also found at stage II. The LD50 at stage II selection was resulted from 1.5 Krad irradiation dose with 15-30 ppm at fusaric acid. Generally, the abaca cultures treated by gamma-ray irradiation more resistant to F. oxysporum than without irradiation. This indicates that irradiation treatment can be used to produces new resistant abaca clones to F. oxysporum.
    @article{Sukmadjaja02p276,
    title = {{Seleksi Silang Tunas Abaka dengan Asam Fusarat atau Filtrat F. Oxysporum dan Regenerasinya Membentuk Planlet}},
    author = {D. Sukmadjaja and I. Mariska and E. G. Lestari and M. Kosmiatin and M. Tombe and Hobir},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {276-288},
    year = {2002},
    abstract = {Gamma-ray irradiation as a mutagen was used to increased somaclonal variation on abaca in vitro culture. Abaca calli were exposed to 0, 0.5, 1.0, 1.5, 2.0, and 3.0 Krad of gamma rays. The experiment results showed that callus growth capacity decreased with increasing dosages of gamma rays irradiation. Callus capacity to regenerate stopped at 3.0 Krad of irradiation dose. The LD50 was found between 1.0-1.5 Krad of irradiaton dose. To obtain new clones of resistant abaca to F. oxysporum, in vitro selection was treated by using two kind selection components with different concentrations: pure toxin of fusaric acid (1, 15, 30, 45, 60, and 75 ppm) and F. oxysporum filtrate (10, 30, and 50%). Selection was carried out in two stages. Concentration of selection components in stages II increased one level to concentration in stage I. The LD50 of selection in stage I was found at 1.0 Krad of irradiation dose with 15 ppm of fusaric acid as selection component. At stage I selection showed that a higher irradiation dose and selection components decreases shoot regeneration capacity. The highest number and height of shoot were observed from the 1.0 Krad of irradiation dose at 0 ppm followed by 1.0 Krad of irradiation dose with 15 ppm of fusaric acid as selection component. The similar results were also found at stage II. The LD50 at stage II selection was resulted from 1.5 Krad irradiation dose with 15-30 ppm at fusaric acid. Generally, the abaca cultures treated by gamma-ray irradiation more resistant to F. oxysporum than without irradiation. This indicates that irradiation treatment can be used to produces new resistant abaca clones to F. oxysporum.},
    keywords = {Abaca, Fusarium oxysporum, fusaric acid},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_276-288_deden.pdf}
    }
  29. Manzila, I., H. Rijzaani, and Bahagiawati. 2002. Pemurnian Wereng Coklat Biotipe Laboratorium. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 289-300.
    [BibTeX] [Abstract] [PDF: Pemurnian Wereng Coklat Biotipe Laboratorium ]
    The study of brown planthopper (BPH) biotypes in 1999/2000 indicated that the laboratory BPH biotypes in Balitbio need to be purified. For that reason, this experiment was conducted to purify the BPH biotypes and at the same time to obtain a proper biotype purification technique. The technique was to select reputed biotype-1 BPH individuals using honeydew tests on Mudgo and ASD7. The individuals that showed preferential feeding on a particular rice plant, as judged by the amount of honeydew secreted, were separated and reared on their preferred host. After being reared on their preferred hosts for 3-4 generations, the biotype characteristics of the resulting populations were confirmed using differential and honeydew tests on several differential rice varieties. A purified biotype-1 population was obtained from this experiment. However, the technique used was not able to generate pure BPH biotype-2 and biotype-3 populations. It seems that for generating pure BPH biotypes, the technique needs to be modified.
    @article{Manzila02p289,
    title = {{Pemurnian Wereng Coklat Biotipe Laboratorium}},
    author = {I. Manzila and H. Rijzaani and Bahagiawati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {289-300},
    year = {2002},
    abstract = {The study of brown planthopper (BPH) biotypes in 1999/2000 indicated that the laboratory BPH biotypes in Balitbio need to be purified. For that reason, this experiment was conducted to purify the BPH biotypes and at the same time to obtain a proper biotype purification technique. The technique was to select reputed biotype-1 BPH individuals using honeydew tests on Mudgo and ASD7. The individuals that showed preferential feeding on a particular rice plant, as judged by the amount of honeydew secreted, were separated and reared on their preferred host. After being reared on their preferred hosts for 3-4 generations, the biotype characteristics of the resulting populations were confirmed using differential and honeydew tests on several differential rice varieties. A purified biotype-1 population was obtained from this experiment. However, the technique used was not able to generate pure BPH biotype-2 and biotype-3 populations. It seems that for generating pure BPH biotypes, the technique needs to be modified.},
    keywords = {brown planthopper, biotype purification, differential test, honeydew test},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_289-300_ifamanzila.pdf}
    }
  30. Suwarno, Erwina Lubis, Allidawati, and Sunaryo. 2002. Perbaikan Ketahanan Varietas Padi Sawah dan Gogo terhadap Hawar Daun Bakteri dan Blas melalui Seleksi dengan Markah Molekuler. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 301-310.
    [BibTeX] [Abstract] [PDF: Perbaikan Ketahanan Varietas Padi Sawah dan Gogo terhadap Hawar Daun Bakteri dan Blas melalui Seleksi dengan Markah Molekuler ]
    Bacterial leaf blight and blast are the major diseases on lowland and upland rice. The diseases has many strains and races causing break down of resitance of improved varieties. To solve this problem, breeding foer resistant to BLB was carried out were two isogenic rice lines were developed, to get several cultivar which have to different resistant genes using back cross program. IRBB5, IRBB7, and IRBB21 were used as resistant sources. These isoline have containing Xa5, Xa7, and Xa21 genes, which have effective for BLB strains in Indonesia. Upland rice improvement for blas resistance containing different resistant genes using double cross method. The sources of blast resistance are C101LAC, C105A51, and C104A51 which have effective for blast race in Indonesia.The result of the experiment showed that in Pusakanegara station, four rice lines of Xa5 gave yield 3.97 kg/5 m2 which were significanly higher than both IR64 (3.45 kg/5 m2) and IRBB5 (3.41 kg/5 m2) as checked varieties. While in Muara station five lines of Xa5 yielded about 3.64-3.97 kg/5 m2, significantly higher than IR64 (2.64 kg/5 m2). Among of the lines from two location, three lines yielded higher than IR64, that were Bio10-BC6-1-1-PN-1, Bio8-BC5-MR-27-1-PN-1-1, and Bio8-BC5-MR- 3-5-2-PN-1. Bio8-BC5-MR-27-1-PN-1-1 yielded significantly higher than IR64 in both Muara and Pusakanegara. However in Pusakanegara seven selected lines of Xa7 yielded the same as IR64, but in Muara three lines yielded about 4.03-4.15 kg/5 m2 higher than IR64 (2.64 kg/5 m2), from the two locations four lines of Xa7 yielded more about 0.6 k kg/5 m2 than IR64. Both in Pusakanegara and Muara five lines of Xa5/Xa7 yielded the same as IR65, IRbb5, and IRBB7. Only Bio13-BC5-MR-2-5-PN-3-1 gave highest yield (3.74 kg/5 m2). After inoclation using Xo 94170 in Pusakanegara were selected 17 lines of Xa21 as resistant to moderately resistant to BLB, while in Muara were selected 19 lines of Xa21. In Pusakanegara four lines of Xa21 yielded 4.0 kg/5 m2 higher than IR64 (3.4 kg/5 m2), five lines of Xa21 yielded the same as IR64 (2.72 kg/5 m2) in Muara. In the other hand, the evaluation of upland rice lines showed that 19 selected lines yielded not different from Way Rarem in CV 14.68%. Among them 16 lines also yielded the same as IR64. In spite of that Bio8-BC5-MR-3-5-2- PN-1 (BIO-1) and Bio9-BC5-MR-4-5-KN-5 (BIO-2) had been released as improved varieties in 2001 as Angke and Conde.
    @article{Suwarno02p301,
    title = {{Perbaikan Ketahanan Varietas Padi Sawah dan Gogo terhadap Hawar Daun Bakteri dan Blas melalui Seleksi dengan Markah Molekuler}},
    author = {Suwarno and Erwina Lubis and Allidawati and Sunaryo},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {301-310},
    year = {2002},
    abstract = {Bacterial leaf blight and blast are the major diseases on lowland and upland rice. The diseases has many strains and races causing break down of resitance of improved varieties. To solve this problem, breeding foer resistant to BLB was carried out were two isogenic rice lines were developed, to get several cultivar which have to different resistant genes using back cross program. IRBB5, IRBB7, and IRBB21 were used as resistant sources. These isoline have containing Xa5, Xa7, and Xa21 genes, which have effective for BLB strains in Indonesia. Upland rice improvement for blas resistance containing different resistant genes using double cross method. The sources of blast resistance are C101LAC, C105A51, and C104A51 which have effective for blast race in Indonesia.The result of the experiment showed that in Pusakanegara station, four rice lines of Xa5 gave yield 3.97 kg/5 m2 which were significanly higher than both IR64 (3.45 kg/5 m2) and IRBB5 (3.41 kg/5 m2) as checked varieties. While in Muara station five lines of Xa5 yielded about 3.64-3.97 kg/5 m2, significantly higher than IR64 (2.64 kg/5 m2). Among of the lines from two location, three lines yielded higher than IR64, that were Bio10-BC6-1-1-PN-1, Bio8-BC5-MR-27-1-PN-1-1, and Bio8-BC5-MR- 3-5-2-PN-1. Bio8-BC5-MR-27-1-PN-1-1 yielded significantly higher than IR64 in both Muara and Pusakanegara. However in Pusakanegara seven selected lines of Xa7 yielded the same as IR64, but in Muara three lines yielded about 4.03-4.15 kg/5 m2 higher than IR64 (2.64 kg/5 m2), from the two locations four lines of Xa7 yielded more about 0.6 k kg/5 m2 than IR64. Both in Pusakanegara and Muara five lines of Xa5/Xa7 yielded the same as IR65, IRbb5, and IRBB7. Only Bio13-BC5-MR-2-5-PN-3-1 gave highest yield (3.74 kg/5 m2). After inoclation using Xo 94170 in Pusakanegara were selected 17 lines of Xa21 as resistant to moderately resistant to BLB, while in Muara were selected 19 lines of Xa21. In Pusakanegara four lines of Xa21 yielded 4.0 kg/5 m2 higher than IR64 (3.4 kg/5 m2), five lines of Xa21 yielded the same as IR64 (2.72 kg/5 m2) in Muara. In the other hand, the evaluation of upland rice lines showed that 19 selected lines yielded not different from Way Rarem in CV 14.68%. Among them 16 lines also yielded the same as IR64. In spite of that Bio8-BC5-MR-3-5-2- PN-1 (BIO-1) and Bio9-BC5-MR-4-5-KN-5 (BIO-2) had been released as improved varieties in 2001 as Angke and Conde.},
    keywords = {Bacterial leaf blight, blast, molecular marker},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_301-310_suwarno.pdf}
    }
  31. Reflinur, Masdiar Bustamam, and Dita Agisimanto. 2002. Analisis Segregasi Populasi Galur Inbrida Rekombinan dari Persilangan Danau Tempe x Kencana Bali terhadap Ras Blas Tertentu di Rumah Kaca. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 319-329.
    [BibTeX] [Abstract] [PDF: Analisis Segregasi Populasi Galur Inbrida Rekombinan dari Persilangan Danau Tempe x Kencana Bali terhadap Ras Blas Tertentu di Rumah Kaca ]
    The use of resistant variety is considered as an inexpensive, efficient, and environmentally safe method to control the rice blast disease caused by Pyticularia grisea. This study is designed to evaluate the resistance of 200 recombinant inbreed lines of Danau Tempe and Kencana Bali to two strains of blast fungus, ID-14 and 041. The experiment was conducted in the greenhouse in complete randomized block design (CRBD) with three replications. Present assessment specifying that out of 200 lines tested, 67% (134 lines) were resistant, 34% (64 lines) moderately resistant, and 1% 2 lines) susceptible to strain ID-14. These lines react slightly different to strain 041; wherein 14% (28 lines) resistant, 69.5% (139 lines) moderately susceptible, and 16.5% (33 lines) susceptible.
    @article{Reflinur02p319,
    title = {{Analisis Segregasi Populasi Galur Inbrida Rekombinan dari Persilangan Danau Tempe x Kencana Bali terhadap Ras Blas Tertentu di Rumah Kaca}},
    author = {Reflinur and Masdiar Bustamam and Dita Agisimanto},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {319-329},
    year = {2002},
    abstract = {The use of resistant variety is considered as an inexpensive, efficient, and environmentally safe method to control the rice blast disease caused by Pyticularia grisea. This study is designed to evaluate the resistance of 200 recombinant inbreed lines of Danau Tempe and Kencana Bali to two strains of blast fungus, ID-14 and 041. The experiment was conducted in the greenhouse in complete randomized block design (CRBD) with three replications. Present assessment specifying that out of 200 lines tested, 67% (134 lines) were resistant, 34% (64 lines) moderately resistant, and 1% 2 lines) susceptible to strain ID-14. These lines react slightly different to strain 041; wherein 14% (28 lines) resistant, 69.5% (139 lines) moderately susceptible, and 16.5% (33 lines) susceptible.},
    keywords = {rice blast, recombinan inbreedlines, danau tempe x kencana bali, green house},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_319-329_reflinur.pdf}
    }
  32. Purwanti, H., S. G. Budiarti, Hadiatmi, and Sutoro. 2002. Evaluasi Galur Jagung Tetua yang Tahan dan Rentan terhadap Penyakit Bulai (Peronosclerospora maydis). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 330-342.
    [BibTeX] [Abstract] [PDF: Evaluasi Galur Jagung Tetua yang Tahan dan Rentan terhadap Penyakit Bulai (Peronosclerospora maydis) ]
    The dowing mildew disease is the most important biotic stress in maize production system. Peronosclerospora maydis serious losses of yield of maize in Indonesia. The dow ny mildew was the most damage seriously in central production of maize. The varieties of maize for d ny mildew resistant is expected to produce better improved varieties of ow maize. The dow ny mildew evaluation was limited maize-inbreds resistant and susceptible in field experiment station and screen-house did in Bogor. There are three resistant parents of inbred lines and three of susceptible parents of inbred lines Nei9202 (Thailand), IPB204-1-31-2-4-B (Filipina), and J2-375-1-1-1-1f (Indonesia) were resistant parents inbred lines, G26C25HS45-3-4-1-6-BBBB, CML356 (CIMMYT) were susceptible parents inbred lines. Crossing G26C25HS45-3-4-1-6-BBBB x J2-375-1-1-1-1f was succes s than other crossing inbred lines.
    @article{Purwanti02p330,
    title = {{Evaluasi Galur Jagung Tetua yang Tahan dan Rentan terhadap Penyakit Bulai (Peronosclerospora maydis)}},
    author = {H. Purwanti and S. G. Budiarti and Hadiatmi and Sutoro},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {330-342},
    year = {2002},
    abstract = {The dowing mildew disease is the most important biotic stress in maize production system. Peronosclerospora maydis serious losses of yield of maize in Indonesia. The dow ny mildew was the most damage seriously in central production of maize. The varieties of maize for d ny mildew resistant is expected to produce better improved varieties of ow maize. The dow ny mildew evaluation was limited maize-inbreds resistant and susceptible in field experiment station and screen-house did in Bogor. There are three resistant parents of inbred lines and three of susceptible parents of inbred lines Nei9202 (Thailand), IPB204-1-31-2-4-B (Filipina), and J2-375-1-1-1-1f (Indonesia) were resistant parents inbred lines, G26C25HS45-3-4-1-6-BBBB, CML356 (CIMMYT) were susceptible parents inbred lines. Crossing G26C25HS45-3-4-1-6-BBBB x J2-375-1-1-1-1f was succes s than other crossing inbred lines.},
    keywords = {key words, peronosclerospora maydis, resistant maize inbred line, susceptible maize inbred line},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_330-342_haeni.pdf}
    }
  33. Koswanudin, D., M. Arifin, and Harnoto. 2002. Kompatibilitas SlNPV dengan Ekstrak Biji Mimba untuk Mengendalikan Ulatgrayak pada Kedelai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 343-347.
    [BibTeX] [Abstract] [PDF: Kompatibilitas SlNPV dengan Ekstrak Biji Mimba untuk Mengendalikan Ulatgrayak pada Kedelai ]
    Compatibility of SlNPV with neem seed extracts for the control of army warm on soybean. The study was carried out in laboratory and green hous of Bogor Agriculture Biotechnology and Genetic Resources. Soybean army warm were collected from the field, were reared and multiplied on artificial diet in laboratory. Wilis varieties of soybean were planted in plastic pot. SlNPV were found from Pasuruan, were reareal and multiplied. Parification of SlNPV were done in centrifuge at 3500 rpm. Suspension which have been known its consentration were dilute until were found 102-108 consentration. Neem seed from East Java were washed and then were dried under sun shine during 3 days. Those neem seed were crushed until were found neem seed powder. About 50 g neem seed powder were soaked in 1 l water, were stired and then were admited about 12 hours, and then were filtered. Filtrate were diluted in teepal until its volume 1 l. Stock solution were made 10-1 consentration. Thirty five days old soybean plant were applied componend of SlNPV and extract neem seed solution. The consentration of SlNPV were 3.7.102 to 3.7.108 PiBS/ml were mixed with 10-1 consentration of neem seed extract. Spray volume was 6 ml/plant. The untreated plant were applied with water and neem seed extract solution. Soybean plants were revered with plastic, and then were infested 15 thiral instar larvae of army warm/treatment. Three days after, 10 larvae/treatment were taken and reared on artifivial diet. The development of army warm were abserved. Combination of SlNPV at the rate of 3.7.107 and 3.7.108 PiBS with 10-1 consentration of neem seed extract were compatible and effective to army warm.
    @article{Koswanudin02p343,
    title = {{Kompatibilitas SlNPV dengan Ekstrak Biji Mimba untuk Mengendalikan Ulatgrayak pada Kedelai}},
    author = {D. Koswanudin and M. Arifin and Harnoto},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {343-347},
    year = {2002},
    abstract = {Compatibility of SlNPV with neem seed extracts for the control of army warm on soybean. The study was carried out in laboratory and green hous of Bogor Agriculture Biotechnology and Genetic Resources. Soybean army warm were collected from the field, were reared and multiplied on artificial diet in laboratory. Wilis varieties of soybean were planted in plastic pot. SlNPV were found from Pasuruan, were reareal and multiplied. Parification of SlNPV were done in centrifuge at 3500 rpm. Suspension which have been known its consentration were dilute until were found 102-108 consentration. Neem seed from East Java were washed and then were dried under sun shine during 3 days. Those neem seed were crushed until were found neem seed powder. About 50 g neem seed powder were soaked in 1 l water, were stired and then were admited about 12 hours, and then were filtered. Filtrate were diluted in teepal until its volume 1 l. Stock solution were made 10-1 consentration. Thirty five days old soybean plant were applied componend of SlNPV and extract neem seed solution. The consentration of SlNPV were 3.7.102 to 3.7.108 PiBS/ml were mixed with 10-1 consentration of neem seed extract. Spray volume was 6 ml/plant. The untreated plant were applied with water and neem seed extract solution. Soybean plants were revered with plastic, and then were infested 15 thiral instar larvae of army warm/treatment. Three days after, 10 larvae/treatment were taken and reared on artifivial diet. The development of army warm were abserved. Combination of SlNPV at the rate of 3.7.107 and 3.7.108 PiBS with 10-1 consentration of neem seed extract were compatible and effective to army warm.},
    keywords = { SlNPV, neem seed, extracts, compatibility},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_343-347_dodin.pdf}
    }
  34. Samsudin. 2002. Keefektifan Kombinasi Anagrapha falcifera NPV (AfNPV) dengan Spodoptera litura NPV (SlNPV) untuk Mengendalikan Ulatgrayak Pada Kedelai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 348-351.
    [BibTeX] [Abstract] [PDF: Keefektifan Kombinasi Anagrapha falcifera NPV (AfNPV) dengan Spodoptera litura NPV (SlNPV) untuk Mengendalikan Ulatgrayak Pada Kedelai ]
    The nuclear polyhedrosis viruses isolated from the vegetable loopers Anagrapha falcifera in America (AfNPV) was shown to successfully infect more than one insect species (broad host range). AfNPV isolate has a potentially to improve as NPV blopesticide with high patogenicity and broad host spectrum. Based on this potential, the study of effectiveness of AfNPV with SINPV combination to control soybean caterpillar Spodoptera litura was conducted in the Biopesticide Laboratory, Research Institute for Crop Biotechnology from March to November 2001. Three treatments were conducted on this study, are SINPV, AfNPV, and combination of SlNPV with AfNPV. All of the treatments made as 102 to 107 concentration of virus. The result showed that AfNPV synergism effect with SINPV. Percentage rate of third instars of soybean caterpillar mortality on the combination treatment higher than both AfNPV and SINPV single.
    @article{Samsudin02p348,
    title = {{Keefektifan Kombinasi Anagrapha falcifera NPV (AfNPV) dengan Spodoptera litura NPV (SlNPV) untuk Mengendalikan Ulatgrayak Pada Kedelai}},
    author = {Samsudin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {348-351},
    year = {2002},
    abstract = {The nuclear polyhedrosis viruses isolated from the vegetable loopers Anagrapha falcifera in America (AfNPV) was shown to successfully infect more than one insect species (broad host range). AfNPV isolate has a potentially to improve as NPV blopesticide with high patogenicity and broad host spectrum. Based on this potential, the study of effectiveness of AfNPV with SINPV combination to control soybean caterpillar Spodoptera litura was conducted in the Biopesticide Laboratory, Research Institute for Crop Biotechnology from March to November 2001. Three treatments were conducted on this study, are SINPV, AfNPV, and combination of SlNPV with AfNPV. All of the treatments made as 102 to 107 concentration of virus. The result showed that AfNPV synergism effect with SINPV. Percentage rate of third instars of soybean caterpillar mortality on the combination treatment higher than both AfNPV and SINPV single.},
    keywords = {afnpv, sinpv, spodoptera litura, soybean},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_348-351_samsudin.pdf}
    }
  35. Sudjadi, M. and Tri P. Priyatno. 2002. Mutasi Jamur Patogen Serangga, Hirsutella citriformis Speare pada Suhu Tinggi (35ºC). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 352-355.
    [BibTeX] [Abstract] [PDF: Mutasi Jamur Patogen Serangga, Hirsutella citriformis Speare pada Suhu Tinggi (35ºC) ]
    Study on mutant development of H. citriformis using mutagenic agent, colchicin, has been conducted at Biopesticide Laboratory of Indonesian in the year 2001. Mutation treatments of H. citriformis mycelia suspension were done by colchicin solution at concentration of 0 to 1.5% (w/v) by 0.25% interval for 10 minutes. 100 ml of mixture solutions inoculated on PDAY medium in petridises containing 0.005% streptomycin to the higest one (1.25%) did not killing the mycelia growth and also the number of mycelia colony growth showed not significantly different compared to untreated control, but colchicin affected on synnemata production and positively correlation from 0.25 to 1.0% of concentration, while at 1.25% the mutant did not production at all.
    @article{Sudjadi02p352,
    title = {{Mutasi Jamur Patogen Serangga, Hirsutella citriformis Speare pada Suhu Tinggi (35ºC)}},
    author = {M. Sudjadi and Tri P. Priyatno},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {352-355},
    year = {2002},
    abstract = {Study on mutant development of H. citriformis using mutagenic agent, colchicin, has been conducted at Biopesticide Laboratory of Indonesian in the year 2001. Mutation treatments of H. citriformis mycelia suspension were done by colchicin solution at concentration of 0 to 1.5% (w/v) by 0.25% interval for 10 minutes. 100 ml of mixture solutions inoculated on PDAY medium in petridises containing 0.005% streptomycin to the higest one (1.25%) did not killing the mycelia growth and also the number of mycelia colony growth showed not significantly different compared to untreated control, but colchicin affected on synnemata production and positively correlation from 0.25 to 1.0% of concentration, while at 1.25% the mutant did not production at all.},
    keywords = {h, citriformis, synnemata, colchicin, mutation},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_352-355_sudjadi.pdf}
    }
  36. Richana, Nur and Ahmad Thontowi. 2002. Kajian Ekspresi Gen $\alpha$ -amilase untuk Mendapatkan Isolat Bakteri Rekombinan Pembawa Gen $\alpha$-amilase. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 356-364.
    [BibTeX] [Abstract] [PDF: Kajian Ekspresi Gen $\alpha$ -amilase untuk Mendapatkan Isolat Bakteri Rekombinan Pembawa Gen $\alpha$-amilase ]
    The purpose of this research were to selected positive colonies that including $\alpha$amylase genes and to make over expression system. Detection of recombinant cells was $\alpha$-complementation test. Steps of over expression system were isolated pUKC 815 plasmid DNA, restricted with BamHI, electroeluted and ligated of 8000 pb pUKC 815 plasmid fragment, and transformed into Escherecia coli DH5$\alpha$. The results indicated were two recombinat colonies that including $\alpha$-amylase genes. Until now, over expression system was prepared pUKC 815 plasmid as expression vector for $\alpha$-amylase genes.
    @article{NurRichana02p356,
    title = {{Kajian Ekspresi Gen $\alpha$ -amilase untuk Mendapatkan Isolat Bakteri Rekombinan Pembawa Gen $\alpha$-amilase}},
    author = {Nur Richana and Ahmad Thontowi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {356-364},
    year = {2002},
    abstract = {The purpose of this research were to selected positive colonies that including $\alpha$amylase genes and to make over expression system. Detection of recombinant cells was $\alpha$-complementation test. Steps of over expression system were isolated pUKC 815 plasmid DNA, restricted with BamHI, electroeluted and ligated of 8000 pb pUKC 815 plasmid fragment, and transformed into Escherecia coli DH5$\alpha$. The results indicated were two recombinat colonies that including $\alpha$-amylase genes. Until now, over expression system was prepared pUKC 815 plasmid as expression vector for $\alpha$-amylase genes.},
    keywords = {expression, $\alpha$-amylase gene},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_356-364_nurrichana.pdf}
    }
  37. Richana, N., A. Thontowi, and P. Lestina. 2002. Teknik Produksi Amilase Skala Pilot dari Isolat Rekombinan Pembawa Gen Amilase. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 365-372.
    [BibTeX] [Abstract] [PDF: Teknik Produksi Amilase Skala Pilot dari Isolat Rekombinan Pembawa Gen Amilase ]
    The purpose of this research was study of amylase production of recombinant isolates that including $\alpha$-amylase genes. The results indicated were mesophilic two recombinat colonies. According Monod model, specific growth velocity of the recombinants isolates was very decreasing, the biomass remdement either product rendement based on the dissolved protein was increasing of recombinant isolates Y x/s = 0.32 and 0.23 g/g and Yp/s = 0.35 and 0.23 g/g of TMII-10-1 and TMII-10-2 isolates are respectively. Even though that not increasing the amylase production.
    @article{Richana02p365,
    title = {{Teknik Produksi Amilase Skala Pilot dari Isolat Rekombinan Pembawa Gen Amilase}},
    author = {N. Richana and A. Thontowi and P. Lestina},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {365-372},
    year = {2002},
    abstract = {The purpose of this research was study of amylase production of recombinant isolates that including $\alpha$-amylase genes. The results indicated were mesophilic two recombinat colonies. According Monod model, specific growth velocity of the recombinants isolates was very decreasing, the biomass remdement either product rendement based on the dissolved protein was increasing of recombinant isolates Y x/s = 0.32 and 0.23 g/g and Yp/s = 0.35 and 0.23 g/g of TMII-10-1 and TMII-10-2 isolates are respectively. Even though that not increasing the amylase production.},
    keywords = {amylase, production, recombinant},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_365-372_nurrichana.pdf}
    }

2001

  1. Somantri, I. H., A. Apriana, I. S. Dewi, E. Listanto, A. D. Ambarwati, and T. J. Santoso. 2001. Menuju Perakitan Tanaman Padi Transgenik Tahan Hama Penggerek Batang. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 1-9.
    [BibTeX] [Abstract] [PDF: Menuju Perakitan Tanaman Padi Transgenik Tahan Hama Penggerek Batang ]
    Rice stemborer (Scirpophaga sp., Lepidoptera) is considered as one of primary pests causing yield decrease in Indonesia. Rice resistant to stemborer is needed to solve this problem. Source of resistant to stemborer has not been found in rice germplasm. It is known that cry gene from Bacillus thuringiensis produced crystal protein toxic to Lepidopteran. In this case, recombinant DNA technology is required to obtain transgenic rice (T0) resistant to stemborer. Two activities were conducted in this research (1998/99). They were (1) rice transformation using microprojectil bombardment and (2) evaluation of putative transgenic (T0) plants. Materials used in the first experiment were Bengawan Solo, Rojolele, Asemandi, and Taipei-309 (control), while gene constructs used were pSBB (35S, cry IA(b)), pUBB (ubiquitin, cry IA(b), pUBC (ubiquitin, cry IA(c)), and constructs containing marker gene Gus and hph (pRQ6), or Gus and Bar (pBar) under control of 35S promoter. Co-transformation system used molarity of 4 : 1 which was a comparison between construct containing gene of interest (cry IA gene) and the contstruct containing molecular marker based on their molarity. In the second experiment, plantlets derived from calli perviously in R hygromycine or Basta /biolaphos selection media were evaluated using Gus assay. 118 green plantlets (T0) were regenerated from Taipei-309 and 73 green plantlets from Asemandi. Gus gene expression of T0 plants derived-calli bombarded with pRQ6/pUBB indicated that 66% of Taipei-309 and 25.6% of Asemandi were positivecontaining pRQ6. The experiment is still on going to evaluated all the T0 plants and perform bioassay using the stemborer.
    @article{Somantri01p1,
    title = {{Menuju Perakitan Tanaman Padi Transgenik Tahan Hama Penggerek Batang}},
    author = {I. H. Somantri and A. Apriana and I. S. Dewi and E. Listanto and A. D. Ambarwati and T. J. Santoso},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {1-9},
    year = {2001},
    abstract = {Rice stemborer (Scirpophaga sp., Lepidoptera) is considered as one of primary pests causing yield decrease in Indonesia. Rice resistant to stemborer is needed to solve this problem. Source of resistant to stemborer has not been found in rice germplasm. It is known that cry gene from Bacillus thuringiensis produced crystal protein toxic to Lepidopteran. In this case, recombinant DNA technology is required to obtain transgenic rice (T0) resistant to stemborer. Two activities were conducted in this research (1998/99). They were (1) rice transformation using microprojectil bombardment and (2) evaluation of putative transgenic (T0) plants. Materials used in the first experiment were Bengawan Solo, Rojolele, Asemandi, and Taipei-309 (control), while gene constructs used were pSBB (35S, cry IA(b)), pUBB (ubiquitin, cry IA(b), pUBC (ubiquitin, cry IA(c)), and constructs containing marker gene Gus and hph (pRQ6), or Gus and Bar (pBar) under control of 35S promoter. Co-transformation system used molarity of 4 : 1 which was a comparison between construct containing gene of interest (cry IA gene) and the contstruct containing molecular marker based on their molarity. In the second experiment, plantlets derived from calli perviously in R hygromycine or Basta /biolaphos selection media were evaluated using Gus assay. 118 green plantlets (T0) were regenerated from Taipei-309 and 73 green plantlets from Asemandi. Gus gene expression of T0 plants derived-calli bombarded with pRQ6/pUBB indicated that 66% of Taipei-309 and 25.6% of Asemandi were positivecontaining pRQ6. The experiment is still on going to evaluated all the T0 plants and perform bioassay using the stemborer.},
    keywords = {rice transgenic plant, cry gene, rice stemborer},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_1-9_idahanarida.pdf}
    }
  2. Herman, M., S. J. Pardal, E. Listanto, T. I. R. Utami, and D. Damayanti. 2001. Evaluasi Tanaman Kedelai Generasi R1 Hasil Transformasi dengan Gen Proteinase Inhibitor II. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 10-18.
    [BibTeX] [Abstract] [PDF: Evaluasi Tanaman Kedelai Generasi R1 Hasil Transformasi dengan Gen Proteinase Inhibitor II ]
    Research was conducted at Molecular Biology and Genetic Engineering Laboratory and Biosafety Containment of Research Institute for Food Crop Biotechnology Bogor in 2000. Researchs were divided into two activities, i.e (1) bioassay of R1 transgenic soybean plants for pod borer (Etiella zinckenella Treit.) and (2) molecular analysis of molecular analysis of pinII gene on R1 transgenic soybean plants. The objectives were to detect the pinII gene and its expression on R1 transformed soybean plants. One event plant was obtained from the Agrobacterium-mediated transformation coded AT1 (cv. Tidar), while five event plants were obtained from the particle bombardment transformation coded WP1 and WP2 (cv. Wilis) and TP1, TP2, TP3 (cv. Tidar). Those plants were all fertile except TP3. And then those seeds were cultivated for bioassay and molecular analysis of R1 plants. Bioassay of R1 transformed soybean was conducted using neonate larvae of E. zinckenella Treit. Some plants were resistant to this pest. It proved by the low pod/seed damages. The healthy seeds were then collected for further analysis. On the molecular analysis, total genome DNA of R1 transformed soybean plants were extracted from the young leaf samples. These DNA samples were then used for the pinII gene detection using Polymerase Chain Reaction (PCR) technique with specific primers for the pinII gene. PCR result indicated that some samples were positive, i.e. AT1-7, AT1-11, AT1-21, AT1-22, AT1-25, and WP2. Seed from the positive event were collected for further analysis. Key words: Evaluation, R1 soybean plants, transformation, proteinase inhi-bitor II gene, molecular analysis, bioassay.
    @article{Herman01p10,
    title = {{Evaluasi Tanaman Kedelai Generasi R1 Hasil Transformasi dengan Gen Proteinase Inhibitor II}},
    author = {M. Herman and S. J. Pardal and E. Listanto and T. I. R. Utami and D. Damayanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {10-18},
    year = {2001},
    abstract = {Research was conducted at Molecular Biology and Genetic Engineering Laboratory and Biosafety Containment of Research Institute for Food Crop Biotechnology Bogor in 2000. Researchs were divided into two activities, i.e (1) bioassay of R1 transgenic soybean plants for pod borer (Etiella zinckenella Treit.) and (2) molecular analysis of molecular analysis of pinII gene on R1 transgenic soybean plants. The objectives were to detect the pinII gene and its expression on R1 transformed soybean plants. One event plant was obtained from the Agrobacterium-mediated transformation coded AT1 (cv. Tidar), while five event plants were obtained from the particle bombardment transformation coded WP1 and WP2 (cv. Wilis) and TP1, TP2, TP3 (cv. Tidar). Those plants were all fertile except TP3. And then those seeds were cultivated for bioassay and molecular analysis of R1 plants. Bioassay of R1 transformed soybean was conducted using neonate larvae of E. zinckenella Treit. Some plants were resistant to this pest. It proved by the low pod/seed damages. The healthy seeds were then collected for further analysis. On the molecular analysis, total genome DNA of R1 transformed soybean plants were extracted from the young leaf samples. These DNA samples were then used for the pinII gene detection using Polymerase Chain Reaction (PCR) technique with specific primers for the pinII gene. PCR result indicated that some samples were positive, i.e. AT1-7, AT1-11, AT1-21, AT1-22, AT1-25, and WP2. Seed from the positive event were collected for further analysis. Key words: Evaluation, R1 soybean plants, transformation, proteinase inhi-bitor II gene, molecular analysis, bioassay.},
    keywords = {evaluation, R1 soybeanplants, transformation, proteinase inhibitor gene, molecular analysis, bioassay.},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_10-18_herman.pdf}
    }
  3. Ambarwati, A. D., D. W. Utami, D. Damayanti, A. Sisharmini, T. J. Santoso, M. Herman, and Minantyorini. 2001. Transformasi, Studi Molekuler, dan Bioasai Tanaman Ubi Jalar untuk Ketahanan terhadap Hama atau Penyakit. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 19-27.
    [BibTeX] [Abstract] [PDF: Transformasi, Studi Molekuler, dan Bioasai Tanaman Ubi Jalar untuk Ketahanan terhadap Hama atau Penyakit ]
    The production of transgenic sweet potato resistant to pest or virus disease was done by inserting proteinase inhibitor (pinII) gene or coat protein (CP) of Sweet Potato Feathery Mottle Virus (SPFMV) gene into sweet potato genome through transformation technique. Knowledge of transformation, molecular, and bio-assay is therefore important in contributing the success to produce transgenic plant. Experiment was conducted in 2000, including some activities: transforma-tion with pinII and CP-SPFMV gene via particle bombardment and Agrobac-terium tumefaciens, study on basic molecular, and bioassay. Explants from petiole and leaf pieces of sweet potato cv. Jewel were used as target tissues. DNA extraction used the modification of CTAB method and bioassay was conducted on storage roots of putative transgenic plants and non transgenic plants (control). Transformation via particle bombardment with co-transformation of pTwa (pinII, Bar) and pRQ6 (Gus, hpt) have not produced the transformant yet surviving in hygromycin selection (25 mg/l). Transformation via A. tumefaciens produced 18 and six putative transgenic plants transformed with pinII and CP-SPFMV gene, respectively. The quantity of the DNA concentration was about 0.7-1.8 µg/µl. Optimation of PCR was o o o obtained at 95 C for 30 sec, 50 C for 60 sec, and 72 C for 45 sec. Bioassay on 32 storage roots based on the number of punctures, showed the score damaged roots was 4.4-4.5 and the percentage of Cylas mortality on putative transgenic plants (30.8%) was higher than on untransformed plants (15%).
    @article{Ambarwati01p19,
    title = {{Transformasi, Studi Molekuler, dan Bioasai Tanaman Ubi Jalar untuk Ketahanan terhadap Hama atau Penyakit}},
    author = {A. D. Ambarwati and D. W. Utami and D. Damayanti and A. Sisharmini and T. J. Santoso and M. Herman and Minantyorini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {19-27},
    year = {2001},
    abstract = {The production of transgenic sweet potato resistant to pest or virus disease was done by inserting proteinase inhibitor (pinII) gene or coat protein (CP) of Sweet Potato Feathery Mottle Virus (SPFMV) gene into sweet potato genome through transformation technique. Knowledge of transformation, molecular, and bio-assay is therefore important in contributing the success to produce transgenic plant. Experiment was conducted in 2000, including some activities: transforma-tion with pinII and CP-SPFMV gene via particle bombardment and Agrobac-terium tumefaciens, study on basic molecular, and bioassay. Explants from petiole and leaf pieces of sweet potato cv. Jewel were used as target tissues. DNA extraction used the modification of CTAB method and bioassay was conducted on storage roots of putative transgenic plants and non transgenic plants (control). Transformation via particle bombardment with co-transformation of pTwa (pinII, Bar) and pRQ6 (Gus, hpt) have not produced the transformant yet surviving in hygromycin selection (25 mg/l). Transformation via A. tumefaciens produced 18 and six putative transgenic plants transformed with pinII and CP-SPFMV gene, respectively. The quantity of the DNA concentration was about 0.7-1.8 µg/µl. Optimation of PCR was o o o obtained at 95 C for 30 sec, 50 C for 60 sec, and 72 C for 45 sec. Bioassay on 32 storage roots based on the number of punctures, showed the score damaged roots was 4.4-4.5 and the percentage of Cylas mortality on putative transgenic plants (30.8%) was higher than on untransformed plants (15%).},
    keywords = {transformation, molecular, bioassay, pinII, CP-SPFMV, swet potato},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_19-27_dinar.pdf}
    }
  4. Pardal, S. J., T. I. R. Utami, and M. Herman. 2001. Organogenesis dan Embriogenesis Somatik Kedelai secara In Vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 28-36.
    [BibTeX] [Abstract] [PDF: Organogenesis dan Embriogenesis Somatik Kedelai secara In Vitro ]
    Experiments were conducted at Molecular Biology and Genetic Engineering Laboratory of Research Institute for Food Crop Biotechnology, Bogor in 1999 to 2000. The objective of the research was to improve plant regeneration system of soybean resulted from genetic transformation experiments using the proteinase inhibitor II gene construct through particle bombardment method. This experi-ments were part of the soybean transformation for pod borer resistance project. Two kind of the plant regeneration system of soybean were evaluated in this experiments, i.e. through organogenesis and somatic embryogenesis. On the organogenesis system, two kind of media formulation were evaluated. Mature cotyledon as explants were cultured on media MBTD (MS + B5 vitamin + thydia-zuron + 2.4-D) and MBTN (MS + B5 vitamin + thydiazuron + NAA). Result indi-cated that media MBTN was better than MBTD on shoot multiplication. Those shoots were then transferred into rooting media and acclimated on soil successfully. The plants were kept in greenhouse until maturity. While on somatic embryogenesis system, young cotyledon as explants were cultured on four type of media formulations, i.e. MS + B5 vitamin + 2.4-D (1, 1.5, and 2 mg/l) and MS + B5 vitamin + 2 mg/l 2.4-D + 50 mg/l L-asparagine + 50 mg/l Lglutamine. Result indicated that media with 1 or 1.5 mg/l 2.4-D was very good for somatic embryos induction, but it wasn’t give high number of plantlet compare to media with 2 mg/l 2.4-D. Key words: Organogenesis, somatic embryogenesis, soybean, in vitro
    @article{Pardal01p28,
    title = {{Organogenesis dan Embriogenesis Somatik Kedelai secara In Vitro}},
    author = {S. J. Pardal and T. I. R. Utami and M. Herman},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {28-36},
    year = {2001},
    abstract = {Experiments were conducted at Molecular Biology and Genetic Engineering Laboratory of Research Institute for Food Crop Biotechnology, Bogor in 1999 to 2000. The objective of the research was to improve plant regeneration system of soybean resulted from genetic transformation experiments using the proteinase inhibitor II gene construct through particle bombardment method. This experi-ments were part of the soybean transformation for pod borer resistance project. Two kind of the plant regeneration system of soybean were evaluated in this experiments, i.e. through organogenesis and somatic embryogenesis. On the organogenesis system, two kind of media formulation were evaluated. Mature cotyledon as explants were cultured on media MBTD (MS + B5 vitamin + thydia-zuron + 2.4-D) and MBTN (MS + B5 vitamin + thydiazuron + NAA). Result indi-cated that media MBTN was better than MBTD on shoot multiplication. Those shoots were then transferred into rooting media and acclimated on soil successfully. The plants were kept in greenhouse until maturity. While on somatic embryogenesis system, young cotyledon as explants were cultured on four type of media formulations, i.e. MS + B5 vitamin + 2.4-D (1, 1.5, and 2 mg/l) and MS + B5 vitamin + 2 mg/l 2.4-D + 50 mg/l L-asparagine + 50 mg/l Lglutamine. Result indicated that media with 1 or 1.5 mg/l 2.4-D was very good for somatic embryos induction, but it wasn't give high number of plantlet compare to media with 2 mg/l 2.4-D. Key words: Organogenesis, somatic embryogenesis, soybean, in vitro},
    keywords = {organogenesis, somatic embryogenesis, soybean, in vitro},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_28-36_saptowo.pdf}
    }
  5. (Sutrisno, I. H. Somantri, B. Soegiarto, E. Listanto, D. Damayanti, S. G. Budiarti, A. Sisharmini, T. J. Santosa, and I. Altosaar. 2001. Regenerasi Embrio Muda Jagung yang Diintroduksi Plasmid pUBC dan pBarGus. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 37-44.
    [BibTeX] [Abstract] [PDF: Regenerasi Embrio Muda Jagung yang Diintroduksi Plasmid pUBC dan pBarGus ]
    An experiment to regenerate immature embryo bombarded with plasmids of pUBC and pBarGus has been carried out at Research Institute for Food Crop Biotechnology, Bogor in the fiscal year of 1998/99 with the short term aim to develop maize that R survived under the selection of Basta and with the long term objective to develop transgenic Bt-maize resistant to Asian corn borer. A pUBC plasmid contained cry IAc gene and pBarGus plasmid contained Gus and Bar genes were introduced into calli derived from immature embryo of maize with the aid of particle bombardment apparatus. 128 calli derived from immature embryo of Antasena variety and 264 Bisma were bombarded with pBarGus and pUBC plasmids. The two plasmids were bombarded as co-transformation using particle bombardment with two times shooting at a distance of 7 cm with the pressure of 1050 psi. Out of 128 Antasena and 264 Bisma immature embryo bombarded, 15 calli of Antasena and 51 calli of Bisma R survived under the selec-tion of Basta (5 mg/liter). Data shows that 19-20% of immature embryo have been inserted by pBarGus. Twenty four and 25 plantlets could be produced from 15 and 51 calli of Antasena and Bisma, respectively. Each callus could pro-duced 0-1 plantlets. Out of 24-25 plantlets, 9-10 plants were successfully accli-matized to produced kernels. Number of seed on each kernel was vary from 2-581seeds. A total number of seeds produced from 10 plants of Antasena were 1653 seeds and nine plants of Bisma were 205 seeds. Three hundred and two immature embryos of Bisma were also bombarded with pUBC alone (without pBarGus) which
    @article{I01p37,
    title = {{Regenerasi Embrio Muda Jagung yang Diintroduksi Plasmid pUBC dan pBarGus}},
    author = {(Sutrisno and I. H. Somantri and B. Soegiarto and E. Listanto and D. Damayanti and S. G. Budiarti and A. Sisharmini and T. J. Santosa and I. Altosaar},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {37-44},
    year = {2001},
    abstract = {An experiment to regenerate immature embryo bombarded with plasmids of pUBC and pBarGus has been carried out at Research Institute for Food Crop Biotechnology, Bogor in the fiscal year of 1998/99 with the short term aim to develop maize that R survived under the selection of Basta and with the long term objective to develop transgenic Bt-maize resistant to Asian corn borer. A pUBC plasmid contained cry IAc gene and pBarGus plasmid contained Gus and Bar genes were introduced into calli derived from immature embryo of maize with the aid of particle bombardment apparatus. 128 calli derived from immature embryo of Antasena variety and 264 Bisma were bombarded with pBarGus and pUBC plasmids. The two plasmids were bombarded as co-transformation using particle bombardment with two times shooting at a distance of 7 cm with the pressure of 1050 psi. Out of 128 Antasena and 264 Bisma immature embryo bombarded, 15 calli of Antasena and 51 calli of Bisma R survived under the selec-tion of Basta (5 mg/liter). Data shows that 19-20% of immature embryo have been inserted by pBarGus. Twenty four and 25 plantlets could be produced from 15 and 51 calli of Antasena and Bisma, respectively. Each callus could pro-duced 0-1 plantlets. Out of 24-25 plantlets, 9-10 plants were successfully accli-matized to produced kernels. Number of seed on each kernel was vary from 2-581seeds. A total number of seeds produced from 10 plants of Antasena were 1653 seeds and nine plants of Bisma were 205 seeds. Three hundred and two immature embryos of Bisma were also bombarded with pUBC alone (without pBarGus) which},
    keywords = {maize, transformation, pUBC, regeneration},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_37-44_sutrisno.pdf}
    }
  6. Purwanti, H.. 2001. Isolasi DNA Daun Jagung Menggunakan Metode Delaporta dan CIMMYT yang Dimodifikasi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 46-52.
    [BibTeX] [Abstract] [PDF: Isolasi DNA Daun Jagung Menggunakan Metode Delaporta dan CIMMYT yang Dimodifikasi ]
    DNA extraction from maize leaves were done using two modified methods of Delaporta from Delaporta University and CIMMYT in the Biology Molecular Laboratory of Research Institute for Food Crop Biotechnology, Bogor and the Genetic Laboratory of the Institute of Plant Breeding, UPLB, Philippines. The study was aimed to obtained a proper method to prepare pure DNA isolates that will be used in another research activity. DNA from the maize leaves were successfully isolated using the modified method of Delaporta, which was usually used to extracted DNA from rice leaf in Delaporta University. Quality of the isolated DNA, however, was not good, since most of the DNAs were degraded. DNA isolation using the modified method of CIMMYT resulted in a better quality of DNAs. PCR amplification or enzyme restriction of the DNA samples will be done following an RFLP analysis. Key words: DNA extraction, maize leaves, Delaporta method, CIMMYT method
    @article{Purwanti01p46,
    title = {{Isolasi DNA Daun Jagung Menggunakan Metode Delaporta dan CIMMYT yang Dimodifikasi}},
    author = {H. Purwanti},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {46-52},
    year = {2001},
    abstract = {DNA extraction from maize leaves were done using two modified methods of Delaporta from Delaporta University and CIMMYT in the Biology Molecular Laboratory of Research Institute for Food Crop Biotechnology, Bogor and the Genetic Laboratory of the Institute of Plant Breeding, UPLB, Philippines. The study was aimed to obtained a proper method to prepare pure DNA isolates that will be used in another research activity. DNA from the maize leaves were successfully isolated using the modified method of Delaporta, which was usually used to extracted DNA from rice leaf in Delaporta University. Quality of the isolated DNA, however, was not good, since most of the DNAs were degraded. DNA isolation using the modified method of CIMMYT resulted in a better quality of DNAs. PCR amplification or enzyme restriction of the DNA samples will be done following an RFLP analysis. Key words: DNA extraction, maize leaves, Delaporta method, CIMMYT method},
    keywords = {dna extraction, maize leaves, delaporta method, cimmyt method},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_46-52_haeni.pdf}
    }
  7. Suwarno, E. Lubis, Alidawati, I. H. Somantri, Minantyorini, and M. Bustamam. 2001. Perbaikan Varietas Padi melalui Seleksi dengan Markah Molekuler dan Kultur Anter. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 53-62.
    [BibTeX] [Abstract] [PDF: Perbaikan Varietas Padi melalui Seleksi dengan Markah Molekuler dan Kultur Anter ]
    Marker aided selection has been applied for bacterial leaf blight (BLB) resistance of lowland rice and blast resistance of upland rice (Oryza sativa L.). Whereas, anther culture was applied for varietal improvement of tidal swamp rice and development of ideal type of lowland rice. The goals of the research are (1) lowland rice varieties similar to IR64 but resistant to BLB, (2) improved upland rice variety with two blast resistance genes, (3) improved variety of tidal swamp rice adapted to acid sulphate and peat soils, and (4) improved variety of lowland rice with ideal plant type. The improvement of resistance to BLB was conducted by incorporating of resistance genes xa5, Xa7, and Xa21 into IR64 with backcross method. The improvement of blast resistance on upland rice was conducted through double cross including two genetic sources for blast resistance genes Pi1 and Pi2 followed by pedigree selection. Varietal improvement for tidal swamp rice was carried out through anther culture applied to plants derived from crosses among selected genetic sources with Al and Fe toxicity tolerance. The development of lowland rice variety with ideal plant type was conducted through anther culture of selected F2 plants derived from crosses among genetic sources with one or more ideal characteristics. Two promising lines similar to IR64 but resistant to BLB have been selected from BC5 derived lines. They are BIO-1 (BIO8-BC5-MR-3-5-2-PN-1) containing xa5 gene and BIO-2 (BIO9-BC5-MR-4-5-KN-5) containing Xa7 gene. Backcrossing to incorporate Xa21 gene into IR64 is in progress at the phase of BC5. Under the improvement of upland rice variety for resistance to blast, some breeding limens containing two resistance genes Pi1 and Pi2, resistant to blast races 15 and 26, and performed good agronomic characteristics have been selected. Some anther-culture-derived breeding lines of tidal swamp rice with tolerance to Fe and Al toxicity, resistance to brown plant hopper (BPH), and good phenotypic acceptability were obtained. Under the development of ideal plant type of lowland rice variety, some breeding lines carrying ideal characteristics were selected.
    @article{Bustamam01p53,
    title = {{Perbaikan Varietas Padi melalui Seleksi dengan Markah Molekuler dan Kultur Anter}},
    author = {Suwarno and E. Lubis and Alidawati and I.H. Somantri and Minantyorini and M. Bustamam},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {53-62},
    year = {2001},
    abstract = {Marker aided selection has been applied for bacterial leaf blight (BLB) resistance of lowland rice and blast resistance of upland rice (Oryza sativa L.). Whereas, anther culture was applied for varietal improvement of tidal swamp rice and development of ideal type of lowland rice. The goals of the research are (1) lowland rice varieties similar to IR64 but resistant to BLB, (2) improved upland rice variety with two blast resistance genes, (3) improved variety of tidal swamp rice adapted to acid sulphate and peat soils, and (4) improved variety of lowland rice with ideal plant type. The improvement of resistance to BLB was conducted by incorporating of resistance genes xa5, Xa7, and Xa21 into IR64 with backcross method. The improvement of blast resistance on upland rice was conducted through double cross including two genetic sources for blast resistance genes Pi1 and Pi2 followed by pedigree selection. Varietal improvement for tidal swamp rice was carried out through anther culture applied to plants derived from crosses among selected genetic sources with Al and Fe toxicity tolerance. The development of lowland rice variety with ideal plant type was conducted through anther culture of selected F2 plants derived from crosses among genetic sources with one or more ideal characteristics. Two promising lines similar to IR64 but resistant to BLB have been selected from BC5 derived lines. They are BIO-1 (BIO8-BC5-MR-3-5-2-PN-1) containing xa5 gene and BIO-2 (BIO9-BC5-MR-4-5-KN-5) containing Xa7 gene. Backcrossing to incorporate Xa21 gene into IR64 is in progress at the phase of BC5. Under the improvement of upland rice variety for resistance to blast, some breeding limens containing two resistance genes Pi1 and Pi2, resistant to blast races 15 and 26, and performed good agronomic characteristics have been selected. Some anther-culture-derived breeding lines of tidal swamp rice with tolerance to Fe and Al toxicity, resistance to brown plant hopper (BPH), and good phenotypic acceptability were obtained. Under the development of ideal plant type of lowland rice variety, some breeding lines carrying ideal characteristics were selected.},
    keywords = {rice, molecular marker, anther culture},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_53-62_suwarno.pdf}
    }
  8. Sutoro, Hadiatmi, S. B. Gajatri, H. Purwanti, and Nurhayati. 2001. Evaluasi dan Identifikasi Markah Molekuler untuk Sifat Tahan Penyakit Bulai dan Heterosis pada Tanaman Jagung. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 69-78.
    [BibTeX] [Abstract] [PDF: Evaluasi dan Identifikasi Markah Molekuler untuk Sifat Tahan Penyakit Bulai dan Heterosis pada Tanaman Jagung ]
    {Corn production could be improved by using downy mildew resistance hybrids. The downy mildew disease of corn is believed to be caused by Peronosclero-spora maydis (Rac). In the plant breeding activity, selection process to obtain interest lines need much time, labor and cost. Increasing of corn yield could be achived by using corn hybrid. The effect of heterotic is one of the important characteristics to obtain hybrid. Heterotic effect resulted in more vigourous than they parents. This fungi has been identified as the causal organism for the serious damage. The problem in producing hybrid is long process to evaluate or select the combining ability between two parents. To gain more efficient in the selection process, moleculer marker could be used as marker aided selection. Research actvities that have been done are inbreed development of downy mildew resistance of corn, heterotic evaluation of corn hybrids, survey of molecular marker of downy mildew and heterotic effecion corn. Result of hybrid evaluation showed that 4 recombinant inbreed T3 x T8, T8 x T9, T1 x T3, and T3 x T10 show good performance with heterotic effect 99.4-116.7% and grain yield 7.3-7.9 t/ha (T1 = SW2-30-2-1-1-#-2-1-2-#
    @article{danNurhayati01p69,
    title = {{Evaluasi dan Identifikasi Markah Molekuler untuk Sifat Tahan Penyakit Bulai dan Heterosis pada Tanaman Jagung}},
    author = {Sutoro and Hadiatmi and S.B. Gajatri and H. Purwanti and Nurhayati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {69-78},
    year = {2001},
    abstract = {Corn production could be improved by using downy mildew resistance hybrids. The downy mildew disease of corn is believed to be caused by Peronosclero-spora maydis (Rac). In the plant breeding activity, selection process to obtain interest lines need much time, labor and cost. Increasing of corn yield could be achived by using corn hybrid. The effect of heterotic is one of the important characteristics to obtain hybrid. Heterotic effect resulted in more vigourous than they parents. This fungi has been identified as the causal organism for the serious damage. The problem in producing hybrid is long process to evaluate or select the combining ability between two parents. To gain more efficient in the selection process, moleculer marker could be used as marker aided selection. Research actvities that have been done are inbreed development of downy mildew resistance of corn, heterotic evaluation of corn hybrids, survey of molecular marker of downy mildew and heterotic effecion corn. Result of hybrid evaluation showed that 4 recombinant inbreed T3 x T8, T8 x T9, T1 x T3, and T3 x T10 show good performance with heterotic effect 99.4-116.7% and grain yield 7.3-7.9 t/ha (T1 = SW2-30-2-1-1-#-2-1-2-#, T3 = J1-46-2-2-9-f, T8 = GM19, T9 = Arc 1-178-1-4-1-3-1-1-1-#, T10 = Hy1). There are interaction between location and hybrid performance. The amount of primer used is 27 primers and resulted in 5 primers showed polymorphism among resistance and suceptible to downy mildew inbreed lines. The primer showed polimorphism are Phi 061, Phi 022, Phi 021, Bngl 589, Nc 132. PCR optimization using SSR marker have been done with 7 primer, Bngl128, Phi 115, Bngl 198, Bngl 657, Bngl 127, Bngl 589, Bngl 371 to evaluate genetic distance between inbreed line. Genetic distance between two inbreed line have been calculated. According to the 7 primers that have been used, it seems there is no relationship between genetic distance and heterotic effect, but this study will be continued by using more primers.},
    keywords = {Zea mays L., downy mildew, heterotic, molecular marker},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_69-78_sutoro.pdf}
    }
  9. Hutami, Mariska I. S., M. Kosmiatin, S. Rahayu, and W. H. Adil. 2001. Regenerasi Massa Sel Embrionik Tanaman Kedelai setelah Diseleksi dengan Al dan pH Rendah. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 79-88.
    [BibTeX] [Abstract] [PDF: Regenerasi Massa Sel Embrionik Tanaman Kedelai setelah Diseleksi dengan Al dan pH Rendah ]
    Soybean variety adapted to acid soil is still limited. One of efforts of producing new genotypes tolerant to acid soil is in vitro selection. Cell mass produced from explants (cotyledone or young zygotics) were selected by treating them with AlCl3.6H2O (0–500 ppm) in pH 4. Varieties used in this study were Wilis, Sindoro and Slamet. Culture medium used was Murashige and Skoog modified by reducing some macro nutrient and Fe was not chelated. To increase genetic variability the explants were irradiated (0 – 400 rad) and reirradiated with 5 or 10 krad. Results showed that Wilis irradiated with 400 rad and selected with Al in low pH produced the largest number of somatic seed (69) followed by Sindoro (55). The variety Slamet irradiated with 400 rad and selected with Al in low pH produced the least somatic seed (13). High irradiation intensity resulted in the cell mass killed after selection. Maturation and germination processes were not uniformly developed.
    @article{Adil01p79,
    title = {{Regenerasi Massa Sel Embrionik Tanaman Kedelai setelah Diseleksi dengan Al dan pH Rendah}},
    author = {Hutami and S., I. Mariska and M. Kosmiatin and S. Rahayu and W.H. Adil},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {79-88},
    year = {2001},
    abstract = {Soybean variety adapted to acid soil is still limited. One of efforts of producing new genotypes tolerant to acid soil is in vitro selection. Cell mass produced from explants (cotyledone or young zygotics) were selected by treating them with AlCl3.6H2O (0–500 ppm) in pH 4. Varieties used in this study were Wilis, Sindoro and Slamet. Culture medium used was Murashige and Skoog modified by reducing some macro nutrient and Fe was not chelated. To increase genetic variability the explants were irradiated (0 – 400 rad) and reirradiated with 5 or 10 krad. Results showed that Wilis irradiated with 400 rad and selected with Al in low pH produced the largest number of somatic seed (69) followed by Sindoro (55). The variety Slamet irradiated with 400 rad and selected with Al in low pH produced the least somatic seed (13). High irradiation intensity resulted in the cell mass killed after selection. Maturation and germination processes were not uniformly developed.},
    keywords = {Glycine max, seleksi in vitro, acid soil, alumunium, cell mass},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_79-88_srihutami.pdf}
    }
  10. Sunarlim, Novianti, Mia Kosmiatin, Ika Mariska, Hadiatmi, Ika Roostika Tambunan, and Suci Rahayu. 2001. Penyimpanan Tanaman Ubi-ubian dengan Metode Pertumbuhan Minimal dan Kriopreservasi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 89-100.
    [BibTeX] [Abstract] [PDF: Penyimpanan Tanaman Ubi-ubian dengan Metode Pertumbuhan Minimal dan Kriopreservasi ]
    The purpose of the experiments were to conserve the tuber crops germplasm on in vitro culture in the laboratory in order to observe the plants easier and the space to store them is relatively smaller. Sweet potatoes (Ipomoea batatas) and cassava (Manihot utilisima) also were conserved with cryopreservation. The method for cryopreservation was vitrification with cryoprotectants of PVS1, PVS2, UBI, M (MS + sucrose + manitol), dan V (VKM + sukrose + manitol). For cassava regeneration trials, BA, AgNO3, and amino acids (glutamin and arginin) were applied to the base media of MS and DKW. Yam (Dioscorea alata) was tried to be conserved with minimal growth. Multiplication media tried was MS + kinetin (0, 0,5, 1, 2, and 4 mg/l) or MS with combination between kinetin (0, 1, and 2 mg/l) and IAA (0, 0,5, and 1 mg/l) or MS + BA (0, 0,5, 1, and 2 mg/l). For conservation media, growth retardant of paclobutrazol (0, 1, 3, and 5 mg/l), ancymidol (0, 1, 2, and 3 mg/l) and osmotic regulator of manitol (0, 20, 40, 60, and 80 g/l) were applied to the basic media of MS. Results of the experiments showed that the explants were still green at the pra culture media of V with the cryoprotectant of manitol and sucrose, but this explants can not be regenerated after they have been frozen. Basic medium of DKW was better than MS for regeneration of cassava. Media for multiplication of yam was MS + kinetin 2 mg/l or MS + BA 1 mg/l. Media for conservation of yam was not found yet because the plants was only about 4 months stored. They were needed to be stored over 1 year to found the best media for conservation. Increasing growth retardant decreased the plant height and leaf size, but increased the number of leaves.
    @article{SuciRahayu01p89,
    title = {{Penyimpanan Tanaman Ubi-ubian dengan Metode Pertumbuhan Minimal dan Kriopreservasi}},
    author = {Novianti Sunarlim and Mia Kosmiatin and Ika Mariska and Hadiatmi and
    Ika Roostika Tambunan and Suci Rahayu},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {89-100},
    year = {2001},
    abstract = {The purpose of the experiments were to conserve the tuber crops germplasm on in vitro culture in the laboratory in order to observe the plants easier and the space to store them is relatively smaller. Sweet potatoes (Ipomoea batatas) and cassava (Manihot utilisima) also were conserved with cryopreservation. The method for cryopreservation was vitrification with cryoprotectants of PVS1, PVS2, UBI, M (MS + sucrose + manitol), dan V (VKM + sukrose + manitol). For cassava regeneration trials, BA, AgNO3, and amino acids (glutamin and arginin) were applied to the base media of MS and DKW. Yam (Dioscorea alata) was tried to be conserved with minimal growth. Multiplication media tried was MS + kinetin (0, 0,5, 1, 2, and 4 mg/l) or MS with combination between kinetin (0, 1, and 2 mg/l) and IAA (0, 0,5, and 1 mg/l) or MS + BA (0, 0,5, 1, and 2 mg/l). For conservation media, growth retardant of paclobutrazol (0, 1, 3, and 5 mg/l), ancymidol (0, 1, 2, and 3 mg/l) and osmotic regulator of manitol (0, 20, 40, 60, and 80 g/l) were applied to the basic media of MS. Results of the experiments showed that the explants were still green at the pra culture media of V with the cryoprotectant of manitol and sucrose, but this explants can not be regenerated after they have been frozen. Basic medium of DKW was better than MS for regeneration of cassava. Media for multiplication of yam was MS + kinetin 2 mg/l or MS + BA 1 mg/l. Media for conservation of yam was not found yet because the plants was only about 4 months stored. They were needed to be stored over 1 year to found the best media for conservation. Increasing growth retardant decreased the plant height and leaf size, but increased the number of leaves.},
    keywords = { Ipomoea batatas, Manihot utilisima, Dioscorea alata, in vitro culture, minimal growth, cryopreservation Ipomoea batatas, Manihot utilisima, Dioscorea alata, in vitro culture, minimal growth, cryopreservation},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_89-100_novianti.pdf}
    }
  11. Mariska, I., D. Sukmadjaja, M. Tombe, E. G. Lestari, and M. Kosmiatin. 2001. Peningkatan Ketahanan Tanaman Abaka terhadap Penyakit Layu melalui Kultur In vitro. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 101-108.
    [BibTeX] [Abstract] [PDF: Peningkatan Ketahanan Tanaman Abaka terhadap Penyakit Layu melalui Kultur In vitro ]
    Abacca (Musa textilis) is a potential crop to be developed as it may be used for different purposes. The crop is being developed in a large scale, but in many cases it was found that the crop is frequently infected by Fusarium oxysporum. To produce new genotypes resistant to the disease the cell mass has been selected by using fusaric acid (0-75 ppm) or the filtrate of F. oxysporum as selecting agent. Result showed that regeneration ability of calli was reduced by increasing the concentration by fusaric acid or filtrate. Six weeks after culturing the control (calli without selecting agent) produced 10 shoots, calli treated with filtrate at 10, 30, and 50%, produced 18, 3, and 2 shoots respectively. The same response was found on calli treated with FA, where increased concentration resulted in decreased callus regeneration. Eight weeks after culturing calli treated with FA 75 ppm produced only 1 shoot, and that treated with FA 45% produced 3 shoots. In the recovery process (MS + BA + thidiazuron), shoot from calli treated with FA 60 and 75 ppm killed, while those from control were able to ploriferate.
    @article{Mariska01p101,
    title = {{Peningkatan Ketahanan Tanaman Abaka terhadap Penyakit Layu melalui Kultur In vitro}},
    author = {I. Mariska and D. Sukmadjaja and M. Tombe and E. G. Lestari and M. Kosmiatin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {101-108},
    year = {2001},
    abstract = { Abacca (Musa textilis) is a potential crop to be developed as it may be used for different purposes. The crop is being developed in a large scale, but in many cases it was found that the crop is frequently infected by Fusarium oxysporum. To produce new genotypes resistant to the disease the cell mass has been selected by using fusaric acid (0-75 ppm) or the filtrate of F. oxysporum as selecting agent. Result showed that regeneration ability of calli was reduced by increasing the concentration by fusaric acid or filtrate. Six weeks after culturing the control (calli without selecting agent) produced 10 shoots, calli treated with filtrate at 10, 30, and 50%, produced 18, 3, and 2 shoots respectively. The same response was found on calli treated with FA, where increased concentration resulted in decreased callus regeneration. Eight weeks after culturing calli treated with FA 75 ppm produced only 1 shoot, and that treated with FA 45% produced 3 shoots. In the recovery process (MS + BA + thidiazuron), shoot from calli treated with FA 60 and 75 ppm killed, while those from control were able to ploriferate.},
    keywords = {Musa textilis, seleksi in vitro, Fusarium oxysporum, fusaric acid, filtrat, calli},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_101-108_ikamariska.pdf}
    }
  12. Lestari, E. G., D. Sukmadjaja, I. Mariska, Hobir, M. Tombe, M. Kosmiatin, Y. Rusyadi, and S. Rahayu. 2001. Perbanyakan In Vitro dan Pengujian Lanjutan pada Nomor-nomor Harapan Panili dan Lada yang Tahan Penyakit. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 109-119.
    [BibTeX] [Abstract] [PDF: Perbanyakan In Vitro dan Pengujian Lanjutan pada Nomor-nomor Harapan Panili dan Lada yang Tahan Penyakit ]
    In vitro propagation and further testing of disease resistant clones of pepper and vanilla. Vanilla and pepper are potential export comodity to be developed, but one of the main problem is disease attacked by Fusarium oxysporum in vanilla and Phytopthora capsici in pepper. To disolve the problems experiments were conducted: 1) somaclonal variation by radiation in many stages of develop somatic embryo and seed of vanilla, 2) in vitro selection of pepper with callus selection by medium containing filtrat of P. capsici.. The experiments were conducted since 1996/1997. In the fiscal year of 2000 vanilla clones were tested in Sukamulya and will be planted in Bali (both of the locations polluted by stem rot vanilla disease). Rooting experiment from new numbers of pepper regenerated from selected callus. The Rooting grow in enriched media with IBA. The result of the experiment showed that 23 from 45 number of vanilla that planted in Sukamulya is resistant after tested and will be planted in Bali. Three number from population in Sukamulya were fruit and CBBb number (70) had fruit longer size than that of common. From pepper the experiment showed that using IBA growth regulator produced root better than that of NAA. The rooted clones (4 number) aclimatized in green house.
    @article{Lestari01p109,
    title = {{Perbanyakan In Vitro dan Pengujian Lanjutan pada Nomor-nomor Harapan Panili dan Lada yang Tahan Penyakit}},
    author = {E. G. Lestari and D. Sukmadjaja and I. Mariska and Hobir and M. Tombe and M. Kosmiatin and Y. Rusyadi and S. Rahayu},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {109-119},
    year = {2001},
    abstract = {In vitro propagation and further testing of disease resistant clones of pepper and vanilla. Vanilla and pepper are potential export comodity to be developed, but one of the main problem is disease attacked by Fusarium oxysporum in vanilla and Phytopthora capsici in pepper. To disolve the problems experiments were conducted: 1) somaclonal variation by radiation in many stages of develop somatic embryo and seed of vanilla, 2) in vitro selection of pepper with callus selection by medium containing filtrat of P. capsici.. The experiments were conducted since 1996/1997. In the fiscal year of 2000 vanilla clones were tested in Sukamulya and will be planted in Bali (both of the locations polluted by stem rot vanilla disease). Rooting experiment from new numbers of pepper regenerated from selected callus. The Rooting grow in enriched media with IBA. The result of the experiment showed that 23 from 45 number of vanilla that planted in Sukamulya is resistant after tested and will be planted in Bali. Three number from population in Sukamulya were fruit and CBBb number (70) had fruit longer size than that of common. From pepper the experiment showed that using IBA growth regulator produced root better than that of NAA. The rooted clones (4 number) aclimatized in green house.},
    keywords = {Vanilla, Vanilla planifolia, pepper, Piper nigrum, in vitro selection, somaclonal variation, radiation, Fusarium oxysporum, Phytopthora capsici},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_109-119_endang.pdf}
    }
  13. Suardi, D., E. Lubis, and S. Moeljopawiro. 2001. Daya Tembus Akar Galur Persilangan BC2F2 Varietas Padi Unggul. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 128-136.
    [BibTeX] [Abstract] [PDF: Daya Tembus Akar Galur Persilangan BC2F2 Varietas Padi Unggul ]
    Kepadatan dan kekerasan tanah merupakan salah satu kendala bagi pertumbuhan akar tanaman dan hasil padi. Penelitian daya tembus akar padi di rumah kaca Balitbio Bogor MT 2000 menggunakan lapisan lilin pada ketebalan 4 dan 3 mm sebagai dasar pot. Galur persilangan padi BC2F2 Cisadane x Cabacu sebanyak 200 nomor, IR64 x Cabacu 400 nomor, dan IR64 x IRAT112 400 nomor diuji daya tembus akarnya pada MT 1998, MT 1999, dan MT 2000 menggunakan rancangan acak kelompok. Beberapa galur persilangan menunjukkan daya tembus akar yang relatif tinggi. Jumlah akar, panjang, dan diameter akar serta indeks daya tembus akar cukup tinggi. Galur-galur tersebut perlu diuji lebih lanjut untuk ketahanannya terhadap kekeringan dan potensi hasil. Perakaran galur persilangan Cisadane x Cabacu relatif kurang mampu menembus lapisan lilin ketebalan 4 mm. Jumlah galur yang akarnya menembus lapisan lilin rendah. Sedangkan galur-galur persilangan IR64 x Cabacu memperlihatkan perakarannya mampu menembus lapisan lilin yang paling tinggi. Jumlah akar dan panjang akar yang menembus lilin paling banyak dan panjang dibandingkan dengan galur persilangan IR64 x IRAT112.
    @article{Suardi01p128,
    title = {{Daya Tembus Akar Galur Persilangan BC2F2 Varietas Padi Unggul}},
    author = {D. Suardi and E. Lubis and S. Moeljopawiro},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {128-136},
    year = {2001},
    abstract = { Kepadatan dan kekerasan tanah merupakan salah satu kendala bagi pertumbuhan akar tanaman dan hasil padi. Penelitian daya tembus akar padi di rumah kaca Balitbio Bogor MT 2000 menggunakan lapisan lilin pada ketebalan 4 dan 3 mm sebagai dasar pot. Galur persilangan padi BC2F2 Cisadane x Cabacu sebanyak 200 nomor, IR64 x Cabacu 400 nomor, dan IR64 x IRAT112 400 nomor diuji daya tembus akarnya pada MT 1998, MT 1999, dan MT 2000 menggunakan rancangan acak kelompok. Beberapa galur persilangan menunjukkan daya tembus akar yang relatif tinggi. Jumlah akar, panjang, dan diameter akar serta indeks daya tembus akar cukup tinggi. Galur-galur tersebut perlu diuji lebih lanjut untuk ketahanannya terhadap kekeringan dan potensi hasil. Perakaran galur persilangan Cisadane x Cabacu relatif kurang mampu menembus lapisan lilin ketebalan 4 mm. Jumlah galur yang akarnya menembus lapisan lilin rendah. Sedangkan galur-galur persilangan IR64 x Cabacu memperlihatkan perakarannya mampu menembus lapisan lilin yang paling tinggi. Jumlah akar dan panjang akar yang menembus lilin paling banyak dan panjang dibandingkan dengan galur persilangan IR64 x IRAT112.},
    keywords = {Daya tembus akar, galur persilangan BC2F2, padi},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_128-136_didisuardi.pdf}
    }
  14. Budiarti, S. G., I. H. Somantri, T. S. Silitonga, N. Zuraida, and Minantyorini. 2001. Rejuvenasi dan Karakterisasi Plasma Nutfah Padi Jagung, Kedelai, dan Ubi Kayu. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 137-147.
    [BibTeX] [Abstract] [PDF: Rejuvenasi dan Karakterisasi Plasma Nutfah Padi Jagung, Kedelai, dan Ubi Kayu ]
    Experiments were conducted to rejuvenate, characterize, and conserve food crops germplasm. The experiments were conducted at Muara, Cikeumeuh, and Pusakanegara Research Instalation, in the dry season and wet season of 2000. A number of 980 accessions of rice, 500 accessions of maize, 600 accessions of soybean, and 550 accessions of cassava were rejuvenated. The rice, maize, soybean, and cassava were planted as pedigrees, without replication with plant spacing 25 cm x 25 cm, (4 x 12 plants) in Pusakanegara (830 accessions), and in a screen house of Muara 1 m x 2 m (150 accessions), maize 75 cm x 20 cm (3 rows, 25 plants/row), soybean 50 cm x 15 cm (2 rows, 40 plants/row), cassava 1 m x 0.60 m (8 plants/clone/variety). The results showed that the rice germplasm varied in, number productive tillers, length leaf, filled grain, and 1000 grain-weight. Five hundreds and twenty seven of the rice accessions had days of maturity between 111-130 days, with productive tillers 6-34/hill, plant height 80-179 cm, filled grain 50-407, leaf length 35-77 cm, and 1000 grain weight 10.1-34.3 g. Among the 50 maize accession variation in plant height was between 165-274 cm, days to silk 41-67 days, days to maturity 74-110 days, ear-length 9.4-22 cm, and 300 seed weight between 48-127 g. There were four soybean accessions that had high yields, i.e. GM 326 Si, GM 851 Si, GM 3741, and GM 4836. Five soybeans accesssions had large seeds were GM 113 Si, GM 338, GM 872, GM 118, and GM 3984 Si. Among the soybeans, variation of plant height was between 22.5-67.6 cm, days of flowering 31-54 days, days of maturity 70-107 days, number of branches 1-5, and 100 seed-weight 6-14.9 g. Among the cassava accessions, variation in plant height ranged for 140-306 cm which Gading was the shortest and Valenca was the highest. Number of leaf lobes of cassava ranged from between 5 -9 lobes, with lobe length between 8.0-21 cm. Si Beru, Gumul, GM-3, CMC 33-38-4, and No. 726 have tuber weight >3.0 kg/plant.
    @article{Budiarti01p137,
    title = {{Rejuvenasi dan Karakterisasi Plasma Nutfah Padi Jagung, Kedelai, dan Ubi Kayu}},
    author = {S. G. Budiarti and I. H. Somantri and T. S. Silitonga and N. Zuraida and Minantyorini},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {137-147},
    year = {2001},
    abstract = { Experiments were conducted to rejuvenate, characterize, and conserve food crops germplasm. The experiments were conducted at Muara, Cikeumeuh, and Pusakanegara Research Instalation, in the dry season and wet season of 2000. A number of 980 accessions of rice, 500 accessions of maize, 600 accessions of soybean, and 550 accessions of cassava were rejuvenated. The rice, maize, soybean, and cassava were planted as pedigrees, without replication with plant spacing 25 cm x 25 cm, (4 x 12 plants) in Pusakanegara (830 accessions), and in a screen house of Muara 1 m x 2 m (150 accessions), maize 75 cm x 20 cm (3 rows, 25 plants/row), soybean 50 cm x 15 cm (2 rows, 40 plants/row), cassava 1 m x 0.60 m (8 plants/clone/variety). The results showed that the rice germplasm varied in, number productive tillers, length leaf, filled grain, and 1000 grain-weight. Five hundreds and twenty seven of the rice accessions had days of maturity between 111-130 days, with productive tillers 6-34/hill, plant height 80-179 cm, filled grain 50-407, leaf length 35-77 cm, and 1000 grain weight 10.1-34.3 g. Among the 50 maize accession variation in plant height was between 165-274 cm, days to silk 41-67 days, days to maturity 74-110 days, ear-length 9.4-22 cm, and 300 seed weight between 48-127 g. There were four soybean accessions that had high yields, i.e. GM 326 Si, GM 851 Si, GM 3741, and GM 4836. Five soybeans accesssions had large seeds were GM 113 Si, GM 338, GM 872, GM 118, and GM 3984 Si. Among the soybeans, variation of plant height was between 22.5-67.6 cm, days of flowering 31-54 days, days of maturity 70-107 days, number of branches 1-5, and 100 seed-weight 6-14.9 g. Among the cassava accessions, variation in plant height ranged for 140-306 cm which Gading was the shortest and Valenca was the highest. Number of leaf lobes of cassava ranged from between 5 -9 lobes, with lobe length between 8.0-21 cm. Si Beru, Gumul, GM-3, CMC 33-38-4, and No. 726 have tuber weight >3.0 kg/plant.},
    keywords = {Rejuvenation, characterization, germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_137-147_srigajatri.pdf}
    }
  15. Budiarti, S. G., T. S. Silitonga, S. A. Rais, L. Hakim, and Hadiatmi. 2001. Evaluasi Pengaruh Cekaman Abiotik pada Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 148-162.
    [BibTeX] [Abstract] [PDF: Evaluasi Pengaruh Cekaman Abiotik pada Plasma Nutfah Tanaman Pangan ]
    This research was aimed to find out accessions of food crops germplasm resis-tant or tolerant to drought, Al toxicity, and shading. The material evaluated con-sisted of 50250 germplasm. The research activities were conducted at Cikeu-meuh, Tamanbogo, and Jakenan in the dry and wet season of 2000. Evaluation for drought resistance was done at Jakenan, using 250 rice accessions, 100 maize accessions, 50 sorghum accessions, and 100 groundnut accessions. Tolerant and susceptible varieties of the respected crops were used as check. Evaluation to Al toxicity that was done at Tamanbogo used 250 rice accessions, 150 maize accessions, 50 sorghum accessions, and 100 groundnut accessions. RCBD with two replications were used. The experiment with 50% plant shading was done at Cikeumeuh using of 50 groundnut and mungbean accessions was arranged in a split-plot design, shaded and non-shaded plants were the plots. The results showed that 16 rice accessions, six maize accessions, and eight sorghum accessions were tolerant to drought, and 18 groundnut accessions were moderatelly tolerant. Evaluation for Al toxicity found that 32 rice acces-sions, 11 maize accessions, seven sorghum accessions, and 11 groundnut accessions tolerant. While six groundnut accessions and 14 mungbean acces-sions were tolerant to shading.
    @article{Budiarti01p148,
    title = {{Evaluasi Pengaruh Cekaman Abiotik pada Plasma Nutfah Tanaman Pangan}},
    author = {S. G. Budiarti and T. S. Silitonga and S. A. Rais and L. Hakim and Hadiatmi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {148-162},
    year = {2001},
    abstract = {This research was aimed to find out accessions of food crops germplasm resis-tant or tolerant to drought, Al toxicity, and shading. The material evaluated con-sisted of 50250 germplasm. The research activities were conducted at Cikeu-meuh, Tamanbogo, and Jakenan in the dry and wet season of 2000. Evaluation for drought resistance was done at Jakenan, using 250 rice accessions, 100 maize accessions, 50 sorghum accessions, and 100 groundnut accessions. Tolerant and susceptible varieties of the respected crops were used as check. Evaluation to Al toxicity that was done at Tamanbogo used 250 rice accessions, 150 maize accessions, 50 sorghum accessions, and 100 groundnut accessions. RCBD with two replications were used. The experiment with 50% plant shading was done at Cikeumeuh using of 50 groundnut and mungbean accessions was arranged in a split-plot design, shaded and non-shaded plants were the plots. The results showed that 16 rice accessions, six maize accessions, and eight sorghum accessions were tolerant to drought, and 18 groundnut accessions were moderatelly tolerant. Evaluation for Al toxicity found that 32 rice acces-sions, 11 maize accessions, seven sorghum accessions, and 11 groundnut accessions tolerant. While six groundnut accessions and 14 mungbean acces-sions were tolerant to shading.},
    keywords = {Evaluation, abiotic, food crops germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_148-162_srigajatri.pdf}
    }
  16. Rais, S. A., T. S. Silitonga, S. G. Budiarti, N. Zuraida, and M. Sudjadi. 2001. Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Cekaman Beberapa Faktor Biotik (Hama dan Penyakit). Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 163-174.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Cekaman Beberapa Faktor Biotik (Hama dan Penyakit) ]
    Evaluation on biotic germplasm of food crops. S.A. Rais, T.S. Silitonga, S.G. Budiarti, Nani Z., dan M. Sudjadi. Th. 2000. The research were conducted at green house, and experimental field i.e. IP. Cikeumeuh, IP. Citayam, and Sukabumi during dry and rainy season of 2000. The aim of this research to find out some accession of germplasm of food crops resitance or tolerance to disease of leaf blast or bacterial leaf blight; rust and leaf spot, downy mildew and sweet potato weevil. The scorring method of experiment for HDB and blas by the IRTP and IRRI method, for rust and leaf spot on ground nut by ICRISAT method; for downy mildew by CIMMYT method, and for the sweet potato weevil by CIP method. The number 250 of rice accessions; 250 of groundnut accessions; 70 of sweet potatoes accessions and 200 of corn accessions, were planted. The experiment was arranged in a randomized block design with two replication. The spacing 25 x 25 cm for rice; 40 x 15 cm for groundnut, and 50 x 20 cm for corn. Ferilizer and weeding as recommended each comodity. The result showed that 8 accessions germplasm of rice resistance to HDB group IV (score 2); 2 accessions score 3 and 36 accessions score 4, check susceptible IR64 score 6. For HDB diseases group VIII, we found Pandan Wangi variety IRegst 16330) score 1 ; 9 acessions score 2; 3 accessions score 3 and 8 accessions score 4; check variety IR64 score 7-8. The groundnut germplasm we found 11 accessions tolerant to leafspot disease score 2-4, and 6 accessions resistant-tolerant to rust score 1-3 : The number 60 accessions of corn resistant-high resistance to downy mildew score 0-20% and 11 accessio s of sweet potatoes resistant to weevil score 0.8-1.3.
    @article{Rais01p163,
    title = {{Evaluasi Ketahanan Plasma Nutfah Tanaman Pangan terhadap Cekaman Beberapa Faktor Biotik (Hama dan Penyakit)}},
    author = {S. A. Rais and T. S. Silitonga and S. G. Budiarti and N. Zuraida and M. Sudjadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {163-174},
    year = {2001},
    abstract = {Evaluation on biotic germplasm of food crops. S.A. Rais, T.S. Silitonga, S.G. Budiarti, Nani Z., dan M. Sudjadi. Th. 2000. The research were conducted at green house, and experimental field i.e. IP. Cikeumeuh, IP. Citayam, and Sukabumi during dry and rainy season of 2000. The aim of this research to find out some accession of germplasm of food crops resitance or tolerance to disease of leaf blast or bacterial leaf blight; rust and leaf spot, downy mildew and sweet potato weevil. The scorring method of experiment for HDB and blas by the IRTP and IRRI method, for rust and leaf spot on ground nut by ICRISAT method; for downy mildew by CIMMYT method, and for the sweet potato weevil by CIP method. The number 250 of rice accessions; 250 of groundnut accessions; 70 of sweet potatoes accessions and 200 of corn accessions, were planted. The experiment was arranged in a randomized block design with two replication. The spacing 25 x 25 cm for rice; 40 x 15 cm for groundnut, and 50 x 20 cm for corn. Ferilizer and weeding as recommended each comodity. The result showed that 8 accessions germplasm of rice resistance to HDB group IV (score 2); 2 accessions score 3 and 36 accessions score 4, check susceptible IR64 score 6. For HDB diseases group VIII, we found Pandan Wangi variety IRegst 16330) score 1 ; 9 acessions score 2; 3 accessions score 3 and 8 accessions score 4; check variety IR64 score 7-8. The groundnut germplasm we found 11 accessions tolerant to leafspot disease score 2-4, and 6 accessions resistant-tolerant to rust score 1-3 : The number 60 accessions of corn resistant-high resistance to downy mildew score 0-20% and 11 accessio s of sweet potatoes resistant to weevil score 0.8-1.3.},
    keywords = {Resistance, biotic, germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_163-174_sriasturirais.pdf}
    }
  17. Zuraida, N., T. S. Silitonga, S. A. Rais, S. G. Budiarti, Hadiatmi, and A. Hidayat. 2001. Evaluasi Mutu Gizi Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 175-180.
    [BibTeX] [Abstract] [PDF: Evaluasi Mutu Gizi Plasma Nutfah Tanaman Pangan ]
    Germplasm is the component of biodiversity had a great role and function in plant improvement. Genetic diversity of germplasm is very important to varietal improvement. The good characters can be obtained from plant genetic variability of germplasm as high nutrition qualities content. Analysis of nutrition qualities had done in MTP laboratory, Balitbio, 2000 to amilosa content in rice and maize, fat and protein in soybean and peanut, HCN content in cassava and tanin content in sorghum. The results showed that variation of fat content is 16,02-36,41% and protein content is 16,93-29,65% in peanut. Soybean had fat content variation between 11,74-17,56% and 23,35-38,81% for protein content. Amilose content in maize had variation between 11.45-27.74%. Sorghum had variation of tanin content between 0,10-1,26%. Amilose content in rice had variation between 9,80-28,45%. HCN content in cassava root had variation between 5,15-99,40 ppm and cassava leaf had variation between 11,88-445,90 ppm.
    @article{Zuraida01p175,
    title = {{Evaluasi Mutu Gizi Plasma Nutfah Tanaman Pangan}},
    author = {N. Zuraida and T. S. Silitonga and S. A. Rais and S. G. Budiarti and Hadiatmi and A. Hidayat},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {175-180},
    year = {2001},
    abstract = { Germplasm is the component of biodiversity had a great role and function in plant improvement. Genetic diversity of germplasm is very important to varietal improvement. The good characters can be obtained from plant genetic variability of germplasm as high nutrition qualities content. Analysis of nutrition qualities had done in MTP laboratory, Balitbio, 2000 to amilosa content in rice and maize, fat and protein in soybean and peanut, HCN content in cassava and tanin content in sorghum. The results showed that variation of fat content is 16,02-36,41% and protein content is 16,93-29,65% in peanut. Soybean had fat content variation between 11,74-17,56% and 23,35-38,81% for protein content. Amilose content in maize had variation between 11.45-27.74%. Sorghum had variation of tanin content between 0,10-1,26%. Amilose content in rice had variation between 9,80-28,45%. HCN content in cassava root had variation between 5,15-99,40 ppm and cassava leaf had variation between 11,88-445,90 ppm.},
    keywords = { Nutrition, diversity, food crops germplasm},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_175-180_nanizuraida.pdf}
    }
  18. Koswanudin, D., S. G. Budiarti, and S. A. Rais. 2001. Evaluasi Ketahanan Plasma Nutfah Jagung terhadap Lalat Bibit Atherigona exigua Stein. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 181-188.
    [BibTeX] [Abstract] [PDF: Evaluasi Ketahanan Plasma Nutfah Jagung terhadap Lalat Bibit Atherigona exigua Stein ]
    An experiment was conducted at Cikeumeuh, Bogor, from December 1999 to February 2000. The experiment was set up using a randomised block design with two replicates. One hundred local maize varieties were evaluated for their resistance to seedling fly (Atherigona exigua). Arjuna variety was used as a resistant check while Sadewa was used as a susceptible check. Plants were grown in 1 m x 5 m plots at a 20 cm x 25 cm spacing. Fertilizers were applied accordingby. Four variables, namely plant growth rate, number of insect eggs, insect population, and plant damage were monitored. The maize germplasm were then grouped based on intensity of the plant damage by the insect A. exi-gua as resistant (0-25%), moderately resistant (25-50%), moderately suscep-tible (51-75%), and susceptible (>76%). Results of the experiment showed that nine varieties were susceptible, 10 moderately susceptible, 71 moderately resistant, and 10 resistant to seedling fly (A. exigua).
    @article{Koswanudin01p181,
    title = {{Evaluasi Ketahanan Plasma Nutfah Jagung terhadap Lalat Bibit Atherigona exigua Stein}},
    author = {D. Koswanudin and S. G. Budiarti and S. A. Rais},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {181-188},
    year = {2001},
    abstract = { An experiment was conducted at Cikeumeuh, Bogor, from December 1999 to February 2000. The experiment was set up using a randomised block design with two replicates. One hundred local maize varieties were evaluated for their resistance to seedling fly (Atherigona exigua). Arjuna variety was used as a resistant check while Sadewa was used as a susceptible check. Plants were grown in 1 m x 5 m plots at a 20 cm x 25 cm spacing. Fertilizers were applied accordingby. Four variables, namely plant growth rate, number of insect eggs, insect population, and plant damage were monitored. The maize germplasm were then grouped based on intensity of the plant damage by the insect A. exi-gua as resistant (0-25%), moderately resistant (25-50%), moderately suscep-tible (51-75%), and susceptible (>76%). Results of the experiment showed that nine varieties were susceptible, 10 moderately susceptible, 71 moderately resistant, and 10 resistant to seedling fly (A. exigua).},
    keywords = {Evaluation, maize germplasm, Atherigona exigua},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_181-188_dodin.pdf}
    }
  19. Sutoro, Hadiatmi, S. G. Budiarti, D. Suardi, and Y. Indarwati. 2001. Evaluasi Plasma Nutfah Jagung (Zea mays L.) Terhadap Kekeringan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 189-196.
    [BibTeX] [Abstract] [PDF: Evaluasi Plasma Nutfah Jagung (Zea mays L.) Terhadap Kekeringan ]
    Corn is one of the food crops that have priority in agriculture development in Indonesia. The corn production is still low caused by most of the corn planted by farmers grow in dry marginal land which is low productivity. Corn production could be improved by using drought tolerance crops. Corn breeding program as an effort to obtain high yield variety or hybrid have been done. Corn germplasm collected from introduction or exploration could be used as breeding material for drought tolerance. Therefore evaluation of germplasm is necessary to be done. Evaluation of corn germplasm have been carried out during dry season 1999 in dry region Jakenan, Pati. Ninety accessions of corn germplasm were tested to drought tolerance. Result of evaluation show that accessions which are tolerant to drought, same with Wisanggeni as check and have short maturity are Tuxpeno Seq C6, Tey Drt Tol Synt, Genjah Kertas, Campolaga, and Lokal Madura (No. registration 3652, 3654, 3659).
    @article{Indarwati01p189,
    title = {{Evaluasi Plasma Nutfah Jagung (Zea mays L.) Terhadap Kekeringan}},
    author = {Sutoro and Hadiatmi and S.G. Budiarti and D. Suardi and Y. Indarwati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {189-196},
    year = {2001},
    abstract = { Corn is one of the food crops that have priority in agriculture development in Indonesia. The corn production is still low caused by most of the corn planted by farmers grow in dry marginal land which is low productivity. Corn production could be improved by using drought tolerance crops. Corn breeding program as an effort to obtain high yield variety or hybrid have been done. Corn germplasm collected from introduction or exploration could be used as breeding material for drought tolerance. Therefore evaluation of germplasm is necessary to be done. Evaluation of corn germplasm have been carried out during dry season 1999 in dry region Jakenan, Pati. Ninety accessions of corn germplasm were tested to drought tolerance. Result of evaluation show that accessions which are tolerant to drought, same with Wisanggeni as check and have short maturity are Tuxpeno Seq C6, Tey Drt Tol Synt, Genjah Kertas, Campolaga, and Lokal Madura (No. registration 3652, 3654, 3659).},
    keywords = {Evaluation, Zea mays, drought tolerance},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_189-196_sutoro.pdf}
    }
  20. Sutoro, H. Kurniawan, and M. Setyowati. 2001. Pengembangan Database Plasma Nutfah Tanaman Pangan. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 197-203.
    [BibTeX] [Abstract] [PDF: Pengembangan Database Plasma Nutfah Tanaman Pangan ]
    Many of characterization and evaluation data on food crops germplasm collection have not been documented yet, therefore it is very difficult to search of the accessions which have certain characters. System database of food crops (rice, corn, sorghum, soybean, peanut, mungbean, cassava and sweet potato) have been developed by using dBase IV, MS-EXCELL and MS-ACCESS software. Amount of accession which have been recorded in the com-puter for rice, corn, sorghum, soybean, peanut, mungbean, sweet potato, and cassava are 3111 accession, 477 accession, 174 accession, 475 accession, 165 accession, 1039 accession, 763 accession, and 110 accession. respec- tively. Based on the database achieved, catalog of food crops germplasm collection in RIFCB have been documented. Many characteristics of germplasm have not evaluated yet, therefore, characterization and evaluation should be done intensively in the future.
    @article{Setyowati01p197,
    title = {{Pengembangan Database Plasma Nutfah Tanaman Pangan}},
    author = {Sutoro and H. Kurniawan and M. Setyowati},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {197-203},
    year = {2001},
    abstract = { Many of characterization and evaluation data on food crops germplasm collection have not been documented yet, therefore it is very difficult to search of the accessions which have certain characters. System database of food crops (rice, corn, sorghum, soybean, peanut, mungbean, cassava and sweet potato) have been developed by using dBase IV, MS-EXCELL and MS-ACCESS software. Amount of accession which have been recorded in the com-puter for rice, corn, sorghum, soybean, peanut, mungbean, sweet potato, and cassava are 3111 accession, 477 accession, 174 accession, 475 accession, 165 accession, 1039 accession, 763 accession, and 110 accession. respec- tively. Based on the database achieved, catalog of food crops germplasm collection in RIFCB have been documented. Many characteristics of germplasm have not evaluated yet, therefore, characterization and evaluation should be done intensively in the future.},
    keywords = {Database, germplasm, food crop},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_197-203_sutoro.pdf}
    }
  21. Suyono, M. Iman, Sutrisno, D. Suwenda, and Isak. 2001. Karakterisasi Wereng Batang Coklat Populasi Lapang dengan Varietas Diferensial. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 204-209.
    [BibTeX] [Abstract] [PDF: Karakterisasi Wereng Batang Coklat Populasi Lapang dengan Varietas Diferensial ]
    Brown planthopper (BPH) is one of the potential pests which it often caused damage to paddy rice in Indonesia. Planting popular resistant varieties widely and continuously could stimulate the development of new biotypes. This was due to the ability of BPH to adapt to resistant rice varieties within few seasons. We need to characterize the BPH biotype using differential varieties to know if there was a shift of BPH biotypes in the field. The BPH used in this study was field populations. The differential rice varieties used were (1) TN 1, (2) Pelita, (3) ASD7, (4) Mudgo, (5) Rathu Heenati, (6) Babawee, (7) ARC 10550, (8) Swarnalata, (9) T27, and (10) PTB33. Results of the study showed that the BPH of IR64 population from Madiun, Blitar, Trenggalek, Ponorogo, and Cisadane population from Kebumen are similar in the virulence. To improve the rice resistant varieties against its BPH, the rice varieties of PTB33 and Rathu Heenati can used as donor of the resistant gene.
    @article{danIsak01p204,
    title = {{Karakterisasi Wereng Batang Coklat Populasi Lapang dengan Varietas Diferensial}},
    author = {Suyono and M. Iman and Sutrisno and D. Suwenda and Isak},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {204-209},
    year = {2001},
    abstract = { Brown planthopper (BPH) is one of the potential pests which it often caused damage to paddy rice in Indonesia. Planting popular resistant varieties widely and continuously could stimulate the development of new biotypes. This was due to the ability of BPH to adapt to resistant rice varieties within few seasons. We need to characterize the BPH biotype using differential varieties to know if there was a shift of BPH biotypes in the field. The BPH used in this study was field populations. The differential rice varieties used were (1) TN 1, (2) Pelita, (3) ASD7, (4) Mudgo, (5) Rathu Heenati, (6) Babawee, (7) ARC 10550, (8) Swarnalata, (9) T27, and (10) PTB33. Results of the study showed that the BPH of IR64 population from Madiun, Blitar, Trenggalek, Ponorogo, and Cisadane population from Kebumen are similar in the virulence. To improve the rice resistant varieties against its BPH, the rice varieties of PTB33 and Rathu Heenati can used as donor of the resistant gene.},
    keywords = {Biotype characterization, brown plant hopper, differential varieties},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_204-209_suyono.pdf}
    }
  22. Chaerani, Jumanto Harjosudarmo, Ace M. Suhendar, and Dodin Koswanudin. 2001. Produksi Massal dan Formulasi Nematoda Patogen Serangga (NPS) Steinernema dan Heterorhabditis untuk Pengendalian Penggerek Batang Padi. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 217-228.
    [BibTeX] [Abstract] [PDF: Produksi Massal dan Formulasi Nematoda Patogen Serangga (NPS) Steinernema dan Heterorhabditis untuk Pengendalian Penggerek Batang Padi ]
    Entomopathogenic nematodes (EPN) belonging to the genera Steinernema and Heterorhabditis are effective biocontrol agents for rice stem borers and other insect pests. However, the wide use of EPN is still hampered by inefficient mass production and formulation techniques. The objectives of this study were to search suitable liquid media for mass production of EPN and to obtain formulation technique that able to retain EPN viability and effectiveness after long term storage. Media consisting of 1% yeast extract, 2.5% chicken eggs, 2.5% soyflour, and 1% soyoil produced the highest level of Heterorhabditis indicus PLR2 up to 93,934 infective juveniles/ml media under static culture condition in petri dishes. The effectiveness of H. indicus PLR2 produced from all tested media were low (0- 13.3%) against Tenebrio molitor larvae compared to the effectiveness of nematode produced in vivo (86.7%) due to inefficient harvest technique. Six out of seven tested media yielded a similar level number of Steinernema T96 (50,617-60,917 infective juveniles of /ml media). However, the highest effectiveness up to 89% against T. molitor larva was obtained from Steinernema T96 produced in media consisting of 1% yeast extract, 2.5% chicken egg, 2.5% soyflour, and 1% soyoil. Alginate and sponge formulations were able to maintain H. indicus PLR2 viability up to 61.1% and 52.1%, respectively, after three month storage at 10oC. The effectiveness of H. indicus PLR2 formulated in alginate to T. molitor was 78.3%, which was not significantly different to the effectiveness of fresh- and non-formulated-nematodes (97.5%), whereas those formulated in sponge had lower effectiveness (61.7%). The highest viability rate of Steinernema T96 up to 40.0% was obtained from sponge formulation but its effectiveness to T. molitor decreased to 33.4% compared to the effectiveness of fresh- and non-formulated- nematodes (54.2%). Further improvements on liquid mass production technique are necessary, i.e. searching optimum level of bacterial and nematode inocula, studying appropriate level of aeration rate in fermentor; and improving harvest method to prevent loss of nematode activity and infectivity.
    @article{danDodinKoswanudin01p217,
    title = {{Produksi Massal dan Formulasi Nematoda Patogen Serangga (NPS) Steinernema dan Heterorhabditis untuk Pengendalian Penggerek Batang Padi}},
    author = {Chaerani and Jumanto Harjosudarmo and M. Ace Suhendar and Dodin Koswanudin},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {217-228},
    year = {2001},
    abstract = { Entomopathogenic nematodes (EPN) belonging to the genera Steinernema and Heterorhabditis are effective biocontrol agents for rice stem borers and other insect pests. However, the wide use of EPN is still hampered by inefficient mass production and formulation techniques. The objectives of this study were to search suitable liquid media for mass production of EPN and to obtain formulation technique that able to retain EPN viability and effectiveness after long term storage. Media consisting of 1% yeast extract, 2.5% chicken eggs, 2.5% soyflour, and 1% soyoil produced the highest level of Heterorhabditis indicus PLR2 up to 93,934 infective juveniles/ml media under static culture condition in petri dishes. The effectiveness of H. indicus PLR2 produced from all tested media were low (0- 13.3%) against Tenebrio molitor larvae compared to the effectiveness of nematode produced in vivo (86.7%) due to inefficient harvest technique. Six out of seven tested media yielded a similar level number of Steinernema T96 (50,617-60,917 infective juveniles of /ml media). However, the highest effectiveness up to 89% against T. molitor larva was obtained from Steinernema T96 produced in media consisting of 1% yeast extract, 2.5% chicken egg, 2.5% soyflour, and 1% soyoil. Alginate and sponge formulations were able to maintain H. indicus PLR2 viability up to 61.1% and 52.1%, respectively, after three month storage at 10oC. The effectiveness of H. indicus PLR2 formulated in alginate to T. molitor was 78.3%, which was not significantly different to the effectiveness of fresh- and non-formulated-nematodes (97.5%), whereas those formulated in sponge had lower effectiveness (61.7%). The highest viability rate of Steinernema T96 up to 40.0% was obtained from sponge formulation but its effectiveness to T. molitor decreased to 33.4% compared to the effectiveness of fresh- and non-formulated- nematodes (54.2%). Further improvements on liquid mass production technique are necessary, i.e. searching optimum level of bacterial and nematode inocula, studying appropriate level of aeration rate in fermentor; and improving harvest method to prevent loss of nematode activity and infectivity. },
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_217-228_chaerani.pdf}
    }
  23. Priyatno, Tri Puji, Chaerani, Y. Suryadi, and M. Sudjadi. 2001. Teknik Produksi dan Formulasi Bakteri Kitinolitik untuk Pengendalian Penyakit Karat Kedelai. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 229-235.
    [BibTeX] [Abstract] [PDF: Teknik Produksi dan Formulasi Bakteri Kitinolitik untuk Pengendalian Penyakit Karat Kedelai ]
    Techniques for propagation and formulation of three selected chitinolytic bacteria (isolates 6a, 6m, and 7) were studied to develop biofungicides for the control of soybean rust pathogen (Phakopsora pachyrhizi). Among four media tested, PDYB containing 0.75% yeast extract was the most suitable medium for the cultivation of the bacteria in terms of cost production. Maximum cell densities of the bacteria peaked at 24 hr after incubation. Optimum incubation temperature for isolates 6m and 7 was 32oC whereas for isolate 6a was lower, 24oC. Cell densities of the three isolates reached the highest at 125 rpm agitation speed. Liquid formulation of the chitinolytic bacteria, either applied singly or in combination in screenhouse was more effective than the powder formulation. Liquid formulation mixture isolates of 6m and 7 gave synergistic effect on disease suppression (52.7%) compared to that when each isolate was applied singly.
    @article{Sudjadi01p229,
    title = {{Teknik Produksi dan Formulasi Bakteri Kitinolitik untuk Pengendalian Penyakit Karat Kedelai}},
    author = {Tri Puji Priyatno and Chaerani and Y. Suryadi and M. Sudjadi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {229-235},
    year = {2001},
    abstract = { Techniques for propagation and formulation of three selected chitinolytic bacteria (isolates 6a, 6m, and 7) were studied to develop biofungicides for the control of soybean rust pathogen (Phakopsora pachyrhizi). Among four media tested, PDYB containing 0.75% yeast extract was the most suitable medium for the cultivation of the bacteria in terms of cost production. Maximum cell densities of the bacteria peaked at 24 hr after incubation. Optimum incubation temperature for isolates 6m and 7 was 32oC whereas for isolate 6a was lower, 24oC. Cell densities of the three isolates reached the highest at 125 rpm agitation speed. Liquid formulation of the chitinolytic bacteria, either applied singly or in combination in screenhouse was more effective than the powder formulation. Liquid formulation mixture isolates of 6m and 7 gave synergistic effect on disease suppression (52.7%) compared to that when each isolate was applied singly.},
    keywords = {Chitinolytic bacteria, biofungicide, Phakopsora pachyrhizi},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_229-235_tripuji.pdf}
    }
  24. Widowati, Sri, Lalu Sukarno, Pudji Raharto, and Ahmad Thontowi. 2001. Studi Pengaruh Penambahan Mineral terhadap Aktivitas Protease dari Bacillus circulans 9b3. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 236-244.
    [BibTeX] [Abstract] [PDF: Studi Pengaruh Penambahan Mineral terhadap Aktivitas Protease dari Bacillus circulans 9b3 ]
    One powerlessness of the enzym storage is low stabilities and activities. Several methods are usually used to enhance stabilization and activity, such as minerals or metal ion addition. Other method to increase production and enzyme activity is molecular technique. Effect mineral addition toward enzyme activity is various depending on the kind and mineral concentration. The aims of this research are to study the minerals effect to protease activity, and to isolate of chromosome and plasmid from B. circulans 9b3. Some divalen minerals added are MgSO4, MnSO4, ZnSO4, CaCl2, and FeSO4 in several concentrations i.e. 0.5; 1.0; 1.5; 2.0; and 2.5 mM). Effect of mineral addition to protease activity is calculated as percent to protease control (100%). Pitcher method was used for chromosome isolation, whereas alkalin lysis was used for plasmid isolation. Results showed that Mn and Ca ions have increase of protease activity in all concentration level. The highest activity was reached at 1.5 mM of mineral Ca and Mn addition, i.e. 5 and 31%, respectively. At all concentration level, Mg have decreased the enzyme activity, while other ions addition have uncertaimed effect. B. circulans 9b3 bacteria has approximately 23 kb chromosomal DNA, and contain two plasmids. This research will be continued to digest the genomic DNA with restriction enzimes.
    @article{danAhmadThontowi01p236,
    title = {{Studi Pengaruh Penambahan Mineral terhadap Aktivitas Protease dari Bacillus circulans 9b3}},
    author = {Sri Widowati and Lalu Sukarno and Pudji Raharto and Ahmad Thontowi},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {236-244},
    year = {2001},
    abstract = { One powerlessness of the enzym storage is low stabilities and activities. Several methods are usually used to enhance stabilization and activity, such as minerals or metal ion addition. Other method to increase production and enzyme activity is molecular technique. Effect mineral addition toward enzyme activity is various depending on the kind and mineral concentration. The aims of this research are to study the minerals effect to protease activity, and to isolate of chromosome and plasmid from B. circulans 9b3. Some divalen minerals added are MgSO4, MnSO4, ZnSO4, CaCl2, and FeSO4 in several concentrations i.e. 0.5; 1.0; 1.5; 2.0; and 2.5 mM). Effect of mineral addition to protease activity is calculated as percent to protease control (100%). Pitcher method was used for chromosome isolation, whereas alkalin lysis was used for plasmid isolation. Results showed that Mn and Ca ions have increase of protease activity in all concentration level. The highest activity was reached at 1.5 mM of mineral Ca and Mn addition, i.e. 5 and 31%, respectively. At all concentration level, Mg have decreased the enzyme activity, while other ions addition have uncertaimed effect. B. circulans 9b3 bacteria has approximately 23 kb chromosomal DNA, and contain two plasmids. This research will be continued to digest the genomic DNA with restriction enzimes.},
    keywords = {Mineral, protease, genome, Bacillus ciculans 9b3},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_236-244_widowati_studi.pdf}
    }
  25. Widowati, Sri, D. Andriani, E. I. Riyanti, P. Raharto, and L. Sukarno. 2001. Karakterisasi Fitase dari Bacillus coagulans. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 245-255.
    [BibTeX] [Abstract] [PDF: Karakterisasi Fitase dari Bacillus coagulans ]
    Phytase (mio-inositol hexacysphospate, E.C. 3.1.3.8) is phosphomono esterase which has capability to hydrolyze of phytic acid to be ontophosphate anorganic and phosphate esters from lower mio-inositol. Phytic acid is a phosphate ester which could bond the important minerals (Ca2+, Fe2+, Mg2+) and protein, there-fore reduce of its digestibility. Application of phytase to reduce phytic acid and to increase of digestibility in food products should note the characteristic of enzyme, so it could work an its optimum activity. Phytase used from Bacillus coagulan E.1.4.4 after it pulified by precipitas using amomilim shephate 70% and dialysis. The fraction result from dialysis is characterised. Result show that specific activity phytase 0.8754 U/mg prot with purification 1.83 time. The enzyme has optimum activity at 40oC and pH 6.0. Enzyme activity will reduce when substrate concentration >0.7 mM. Km value from its lineweaver-Burk curve is 0.562 mM, Vmax 0.73 ?mol PO4-3/minute/ml. The minerals Ca2+, Mg2+, Mn2+ have roles as activator for phytase from B. coagulans E1.4.4, while Fe2+ an inhibitor.
    @article{Sukarno01p245,
    title = {{Karakterisasi Fitase dari Bacillus coagulans}},
    author = {Sri Widowati and D. Andriani and E.I. Riyanti and P. Raharto and L. Sukarno},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {245-255},
    year = {2001},
    abstract = { Phytase (mio-inositol hexacysphospate, E.C. 3.1.3.8) is phosphomono esterase which has capability to hydrolyze of phytic acid to be ontophosphate anorganic and phosphate esters from lower mio-inositol. Phytic acid is a phosphate ester which could bond the important minerals (Ca2+, Fe2+, Mg2+) and protein, there-fore reduce of its digestibility. Application of phytase to reduce phytic acid and to increase of digestibility in food products should note the characteristic of enzyme, so it could work an its optimum activity. Phytase used from Bacillus coagulan E.1.4.4 after it pulified by precipitas using amomilim shephate 70% and dialysis. The fraction result from dialysis is characterised. Result show that specific activity phytase 0.8754 U/mg prot with purification 1.83 time. The enzyme has optimum activity at 40oC and pH 6.0. Enzyme activity will reduce when substrate concentration >0.7 mM. Km value from its lineweaver-Burk curve is 0.562 mM, Vmax 0.73 ?mol PO4-3/minute/ml. The minerals Ca2+, Mg2+, Mn2+ have roles as activator for phytase from B. coagulans E1.4.4, while Fe2+ an inhibitor.},
    keywords = {Phytase, Bacillus coagulans, phytate, enzyme characteristics},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2001_245-255_widowati.pdf}
    }
  26. Sisharmini, A., A. D. Ambarwati, T. J. Santoso, D. W. Utami, and M. Herman. 2001. Teknik Isolasi DNA dan Analisis PCR Gen pinII pada Genom Ubi Jalar. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 125-132.
    [BibTeX] [Abstract] [PDF: Teknik Isolasi DNA dan Analisis PCR Gen pinII pada Genom Ubi Jalar ]
    The early confirmation of a target gene in the transformed plants could be very useful because plants that do not contains the target gene can be identified and be discarded early, so that only plants have an interest gene can be used for next observation. One of the techniques to determine the presence and integration of transferred target gene is PCR technique. The technique allows the in vitro synthesis and amplification of spesific fragment of target DNA using oligonucleotide primers so that the target gene can be detected in the genome of transgenic sweet potato. However this technique needed good quality of the DNA extracted. In the year of 2001, the experiment was conducted to identified the presence of pinII gene in the genome of putative transgenic sweet potato. The DNA was isolated from the leaves of putative transgenic plant using two DNA extraction methods i.e. modified CTAB method from Porebski et al. and Varadarajan and Prakash and modified CTAB method from Tanaka and Nakatani. The result showed that modified CTAB method from Tanaka and Nakatani gave the higher purity of DNA compared to the modified CTAB method from Porebski et al. and Varadarajan and Prakash. Based on PCR results, the pinII gene was not detected on the thirteen putative transgenic sweet potatoes.
    @article{Sisharmini01p125,
    title = {{Teknik Isolasi DNA dan Analisis PCR Gen pinII pada Genom Ubi Jalar}},
    author = {A. Sisharmini and A. D. Ambarwati and T. J. Santoso and D. W. Utami and M. Herman},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {125-132},
    year = {2001},
    abstract = {The early confirmation of a target gene in the transformed plants could be very useful because plants that do not contains the target gene can be identified and be discarded early, so that only plants have an interest gene can be used for next observation. One of the techniques to determine the presence and integration of transferred target gene is PCR technique. The technique allows the in vitro synthesis and amplification of spesific fragment of target DNA using oligonucleotide primers so that the target gene can be detected in the genome of transgenic sweet potato. However this technique needed good quality of the DNA extracted. In the year of 2001, the experiment was conducted to identified the presence of pinII gene in the genome of putative transgenic sweet potato. The DNA was isolated from the leaves of putative transgenic plant using two DNA extraction methods i.e. modified CTAB method from Porebski et al. and Varadarajan and Prakash and modified CTAB method from Tanaka and Nakatani. The result showed that modified CTAB method from Tanaka and Nakatani gave the higher purity of DNA compared to the modified CTAB method from Porebski et al. and Varadarajan and Prakash. Based on PCR results, the pinII gene was not detected on the thirteen putative transgenic sweet potatoes.},
    keywords = {sweet potato, pinii gene, pcr identification},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_125-132_atmitri.pdf}
    }
  27. Hadiatmi, Sri G. Budiarti, and Sutoro. 2001. Evaluasi Heterosis Tanaman Jagung. Nature 62 185-194.
    [BibTeX] [Abstract] [PDF: Evaluasi Heterosis Tanaman Jagung ]
    The effect of heterosis is one of the important characteristics to obtain hybrid. The experiment was conducted to measure the heterosis value and effect of combining ability in F1 generation, and also to gain the nature of gene action on each characters. Fiveteen diallel crosses (F1) were made among six parent lines in the dry season of 2001 in Bogor. The material consist of six parent lines, 15 diallel crosses (F1), and two check varieties of Bisma and C were tested in the wet -7 season of 2001 in Bogor, using a randomized complete block design with three replications. Heterosis value for yield of hybrids ranged from 62,4-223,8% more than they mid parent values. The hybrid of J1-19-1-3-1-f/Arc1-178-1-4-1-3-1-1-1- x b was superior hybrid which produced the highest heterosis (223,8%) and grain yield (9,13 t.ha-1). All of hybrid flowered earlier than the midparent value. Line J1-46-2-9f had good general combining ability (gca)/for yield and ear length. Line SW2-30-2-11#2-1-2-# had good gca for flowering date and ear height. Line Arc1-178-1-4-1-3-11-1- x b had good gca for diameter of ear and number of grain rows. Five hybrids showed the heighest heterosis value for yield, produced high grain yield, and had good specific combining ability.
    @article{Hadiatmi01p185,
    title = {{Evaluasi Heterosis Tanaman Jagung}},
    author = {Hadiatmi and Sri G. Budiarti and Sutoro},
    journal = {Nature},
    pages = {185-194},
    volume = {62},
    year = {2001},
    abstract = {The effect of heterosis is one of the important characteristics to obtain hybrid. The experiment was conducted to measure the heterosis value and effect of combining ability in F1 generation, and also to gain the nature of gene action on each characters. Fiveteen diallel crosses (F1) were made among six parent lines in the dry season of 2001 in Bogor. The material consist of six parent lines, 15 diallel crosses (F1), and two check varieties of Bisma and C were tested in the wet -7 season of 2001 in Bogor, using a randomized complete block design with three replications. Heterosis value for yield of hybrids ranged from 62,4-223,8% more than they mid parent values. The hybrid of J1-19-1-3-1-f/Arc1-178-1-4-1-3-1-1-1- x b was superior hybrid which produced the highest heterosis (223,8%) and grain yield (9,13 t.ha-1). All of hybrid flowered earlier than the midparent value. Line J1-46-2-9f had good general combining ability (gca)/for yield and ear length. Line SW2-30-2-11#2-1-2-# had good gca for flowering date and ear height. Line Arc1-178-1-4-1-3-11-1- x b had good gca for diameter of ear and number of grain rows. Five hybrids showed the heighest heterosis value for yield, produced high grain yield, and had good specific combining ability.},
    keywords = {heterosis, combining ability, corn},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2002_185-194_hadiatmi.pdf}
    }
  28. Sisharmini, A., A. D. Ambarwati, T. J. Santoso, and M. Herman. 2001. Analisis Molekuler Integrasi Gen PinII pada Ubi Jalar. Prosiding seminar hasil penelitian rintisan dan bioteknologi tanaman 1 193-201.
    [BibTeX] [Abstract] [PDF: Analisis Molekuler Integrasi Gen PinII pada Ubi Jalar ]
    The early confirmation of integration of a target gene in the transformed plants could be very was conducted to identified the useful because plants that do not contains the target gene can be indentified, so that only plants have a interest gene can be used for next observation. One of techniques to determine the presence and integration of transferred target gene is a PCR technique. Target gene can be detected in the genome of transgenic sweetpotato using PCR technique needed good quality and quantity of the DNA extracted. In the year of 2002, was resulted 26 putative transgenic sweetpotato that transformed with pinII gene and 9 putative transgenic sweetpotato with CP-SPFMV gene, which molecular analysis to confirm integration of pinII gene and CP-SPFMV gene into sweet potato genome. DNA extracted from leaves putative transgenic plants using CTAB method from Tanaka and Nakatani et al. (2001). DNA of putative transgenic plants have purity 1.4-2.26 with quantity 0.009-2.5 µg/µl. PCR analysis from 35 putative trangenic plants showed that no plants containing pinII and CP-SPFMV gene.
    @article{Sisharmini01p193,
    title = {{Analisis Molekuler Integrasi Gen PinII pada Ubi Jalar}},
    author = {A. Sisharmini and A. D. Ambarwati and T. J. Santoso and M. Herman},
    journal = {Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman},
    pages = {193-201},
    volume = {1},
    year = {2001},
    abstract = {The early confirmation of integration of a target gene in the transformed plants could be very was conducted to identified the useful because plants that do not contains the target gene can be indentified, so that only plants have a interest gene can be used for next observation. One of techniques to determine the presence and integration of transferred target gene is a PCR technique. Target gene can be detected in the genome of transgenic sweetpotato using PCR technique needed good quality and quantity of the DNA extracted. In the year of 2002, was resulted 26 putative transgenic sweetpotato that transformed with pinII gene and 9 putative transgenic sweetpotato with CP-SPFMV gene, which molecular analysis to confirm integration of pinII gene and CP-SPFMV gene into sweet potato genome. DNA extracted from leaves putative transgenic plants using CTAB method from Tanaka and Nakatani et al. (2001). DNA of putative transgenic plants have purity 1.4-2.26 with quantity 0.009-2.5 µg/µl. PCR analysis from 35 putative trangenic plants showed that no plants containing pinII and CP-SPFMV gene.},
    keywords = {sweetpotato, extraction of dna, pinii gene, cp-spfmv gene, pcr analysis},
    file = {http://biogen.litbang.pertanian.go.id/terbitan/pdf/prosiding2003_193-201_atmitri_analisis.pdf}
    }